Activating Compound | Comment | Organism | Structure |
---|---|---|---|
bovine serum albumin | 1 mg/ml stimulates activity | Catharanthus roseus |
General Stability | Organism |
---|---|
unstable when highly diluted, 1 M sucrose or 1 mg/ml bovine serum albumin stabilizes activity | Catharanthus roseus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no substrate inhibition | Catharanthus roseus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
tryptamine | pH 6.8, 30°C | Catharanthus roseus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
tryptamine + secologanin | Catharanthus roseus | - |
3-alpha(S)-strictosidine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Catharanthus roseus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | all isoforms, mannose bound to enzyme | Catharanthus roseus |
Purification (Comment) | Organism |
---|---|
six isoforms purified | Catharanthus roseus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Catharanthus roseus | - |
leaf | - |
Catharanthus roseus | - |
seed | - |
Catharanthus roseus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
31.62 | - |
purified enzyme, pH 6.8, 30°C | Catharanthus roseus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
tryptamine + secologanin | - |
Catharanthus roseus | 3-alpha(S)-strictosidine | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Catharanthus roseus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7.5 | 50% activity | Catharanthus roseus |