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Literature summary for 4.3.3.2 extracted from
- Improved expression of His(6)-tagged strictosidine synthase cDNA for chemo-enzymatic alkaloid diversification (2010), Chem. Biodivers., 7, 860-870.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli, coexpression with chaerone pG-Tf2 | Rauvolfia serpentina |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
values between 36000 and 41000, dependent on expression system | Rauvolfia serpentina |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rauvolfia serpentina | P68175 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by Ni-NTA affinity chromatography | Rauvolfia serpentina |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
activities between 14.29 nKat/l and 67.15 nKat/l, depending on expression system, increased activity in presence of chaperone pG-Tf2 | Rauvolfia serpentina |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 7-azatryptamine + secologanin | assay at pH 7.0, 28°C, reaction stopped by adding methanol | Rauvolfia serpentina | 3-alpha(S)-12-azastrictosidine | - |
? |  |
| tryptamine + secologanin | assay at pH 7.0, 28°C, reaction stopped by adding methanol | Rauvolfia serpentina | 3-alpha(S)-strictosidine + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| STR1 | - |
Rauvolfia serpentina |
| stricotsidine synthase | - |
Rauvolfia serpentina |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 28 | - |
assay at | Rauvolfia serpentina |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Rauvolfia serpentina |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | involved in biosynthesis of monoterpenoid indole alkaloids | Rauvolfia serpentina |
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Release 2023.1 (January 2023)
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