Information on EC 2.4.1.152 - 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase

Glycosphingolipid biosynthesis - globo and isoglobo series, Glycosphingolipid biosynthesis - lacto and neolacto series, Mannose type O-glycan biosynthesis

KEGG

MetaCyc

ABH and Lewis epitopes biosynthesis from type 2 precursor disaccharide, biosynthesis of Lewis epitopes (H. pylori), H. pylori 26695 O-antigen biosynthesis

GDP-beta-L-fucose:beta-D-galactosyl-(1->4)-N-acetyl-D-glucosaminyl-R 3-alpha-L-fucosyltransferase (configuration-inverting)

Normally acts on a glycoconjugate where R (see reaction) is a glycoprotein or glycolipid. This enzyme fucosylates on O-3 of an N-acetylglucosamine that carries a galactosyl group on O-4, unlike EC 2.4.1.65, 3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase, which fucosylates on O-4 of an N-acetylglucosamine that carries a galactosyl group on O-3.

111310-38-4

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39279-34-0

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6-deoxy-6-N-(2-naphthalene-2-yl-acetamide)-beta-L-galactopyranos-1-yl-guanosine 5'-diphosphate + sialyl-alpha-2,3-LacNAc-O-(CH2)3-N-dansyl

GDP + Galbeta(1-4)-[6-deoxy-6-N-(2-naphthalene-2-yl-acetamide)-L-Galbeta(1-3)]GlcNAc-O-(CH2)3-N-dansyl

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Substrates: via intermediate 6-azid0-1,2,3,4-tetra-O-benzoyl-6-deoxy-beta-L-galactopyranose, 6-deoxy-6-N-(2-naphalene-2-yl-acetamide)-beta-L-galactopyranos-1-yl-guanosine 5'-diphosphate disodium salt is the most sensitive and selective donor substrate for FUT-VI among all of the GDP-Fuc analogues, including the parent GDP-Fuc, known to date
Products: -

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6-deoxy-6-N-(2-naphthalene-2-yl-acetamide)-beta-L-galactopyranos-1-yl-guanosine 5'-diphosphate disodium salt + dansylated sialyl-alpha2,3-LacNAc

sLex tetrasaccharide

-

Substrates: -
Products: -

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GDP-6,7-dideoxy-beta-L-galacto-hept-6-enopyranose + 2-azidoethyl 2-(acetylamino)-2-deoxy-4-O-beta-D-galactopyranosyl-beta-D-glucopyranoside

GDP + 2-azidoethyl 6,7-dideoxy-beta-L-galacto-hept-6-enopyranosyl-(1-3)-[beta-D-galactopyranosyl-(1-4)]-2-(acetylamino)-2-deoxy-beta-D-glucopyranoside

Substrates: -
Products: -

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GDP-6-azido-6-deoxy-beta-L-galactopyranose + 2-azidoethyl 2-(acetylamino)-2-deoxy-4-O-beta-D-galactopyranosyl-beta-D-glucopyranoside

GDP + 2-azidoethyl -6-azido-6-deoxy-beta-L-galactopyranosyl-(1-3)-[beta-D-galactopyranosyl-(1-4)]-2-(acetylamino)-2-deoxy-beta-D-glucopyranoside

Substrates: -
Products: -

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GDP-6-deoxy-6-fluoro-beta-L-galactopyranose + 2-azidoethyl 2-(acetylamino)-2-deoxy-4-O-beta-D-galactopyranosyl-beta-D-glucopyranoside

GDP + 2-azidoethyl -6-deoxy-6-fluoro-beta-L-galactopyranosyl-(1-3)-[beta-D-galactopyranosyl-(1-4)]-2-(acetylamino)-2-deoxy-beta-D-glucopyranoside

Substrates: -
Products: -

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GDP-6-O-methyl-beta-L-galactopyranose + 2-azidoethyl 2-(acetylamino)-2-deoxy-4-O-beta-D-galactopyranosyl-beta-D-glucopyranoside

GDP + 2-azidoethyl 6-O-methyl-beta-L-galactopyranosyl-(1-3)-[beta-D-galactopyranosyl-(1-4)]-2-(acetylamino)-2-deoxy-beta-D-glucopyranoside

Substrates: -
Products: -

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GDP-alpha-D-(5-cyano)arabinopyranose + 2-azidoethyl 2-(acetylamino)-2-deoxy-4-O-beta-D-galactopyranosyl-beta-D-glucopyranoside

GDP + 2-azidoethyl alpha-D-(5-cyano)arabinopyranosyl-(1-3)-[beta-D-galactopyranosyl-(1-4)]-2-(acetylamino)-2-deoxy-beta-D-glucopyranoside

Substrates: -
Products: -

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GDP-alpha-D-arabinopyranose + 2-azidoethyl 2-(acetylamino)-2-deoxy-4-O-beta-D-galactopyranosyl-beta-D-glucopyranoside

GDP + 2-azidoethyl alpha-D-arabinopyranosyl-(1-3)-[beta-D-galactopyranosyl-(1-4)]-2-(acetylamino)-2-deoxy-beta-D-glucopyranoside

Substrates: -
Products: -

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GDP-alpha-L-fucose + asialofetuin

GDP + ?

GDP-alpha-L-fucose + fetuin

GDP + ?

GDP-alpha-L-fucose + glycoprotein gp130

GDP + ?

GDP-beta-fucose + alpha2,3-sialyl N-acetyllactosamine

GDP + alpha1,3-fucosyl-alpha2,3-sialyl-N-acetyllactosamine

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Substrates: FucT-VII and FucT-IV
Products: -

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GDP-beta-fucose + alpha2,3-sialyl N-acetyllactosaminyl-R

GDP + alpha1,3-fucosyl-alpha2,3-sialyl-N-acetyllactosaminyl-R

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Substrates: FucT-VII and FucT-IV
Products: -

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GDP-beta-fucose + lacto-N-neotetraose

GDP + Galbeta1-4(Fucalpha1-3)GlcNAc

Substrates: -
Products: -

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GDP-beta-fucose + lactose

GDP + alpha1,3-fucosyllactose

Substrates: N-acetyllactosamine is preferred over lactose
Products: -

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GDP-beta-fucose + N-acetyllactosamine

GDP + alpha1,3-fucosyl-N-acetyllactosamine

GDP-beta-L-fucose + 3-sulfo-Galbeta1,4GlcNAc

?

GDP-beta-L-fucose + 4-deoxy-Galbeta1,4GlcNAc

?

GDP-beta-L-fucose + alpha(2,3) bisialylated biantennary glycan

?

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Substrates: -
Products: -

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GDP-beta-L-fucose + asialo-erythropoietin

?

Q5Q0U2

Substrates: wild-type FUT9 efficiently fucosylates di-, tri- and tetraantennary N-glycans from asialoEPO
Products: -

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GDP-beta-L-fucose + beta-D-galactosyl-(1->4)-N-acetyl-D-glucosaminyl-R

GDP + beta-D-galactosyl-(1->4)-[alpha-L-fucosyl-(1->3)]-N-acetyl-beta-D-glucosaminyl-R

-

Substrates: -
Products: -

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GDP-beta-L-fucose + D-lactose

GDP + 3 L-Fuc-alpha-(1->3)-[D-Gal-beta(1->4)]-D-Glc

Substrates: the enzyme has very low relative substrate specificity toward D-lactose
Products: -

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GDP-beta-L-fucose + fetuin

?

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Substrates: -
Products: -

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GDP-beta-L-fucose + fetuin-biotin

?

-

Substrates: -
Products: -

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GDP-beta-L-fucose + Fucalpha1,2Galbeta1,4GlcNAc

?

GDP-beta-L-fucose + Fucalpha1-2Galbeta1-4Glc

GDP + Fucalpha1-2Galbeta1-4Glcalpha1-3Fuc

-

Substrates: -
Products: -

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GDP-beta-L-fucose + Galbeta1,4GlcNAc

?

GDP-beta-L-fucose + Galbeta1-3GlcNAcbeta1-3Galbeta1-4Glc

GDP + Galbeta1-3GlcNAcbeta1-3Galbeta1-4Glcalpha1-3Fuc

GDP-beta-L-fucose + Galbeta1-4Glc

GDP + Galbeta1-4Glcalpha1-3Fuc

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Substrates: -
Products: -

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GDP-beta-L-fucose + Galbeta1-4GlcNAcalpha-4-nitrophenol

GDP + Galbeta1-4(Fucalpha1-3)GlcNAcalpha-4-nitrophenol

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686972

Substrates: preferred substrate of CEFT-4 and CEFT-3
Products: -

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GDP-beta-L-fucose + Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glc

GDP + Galbeta1-4(Fucalpha1-3)GlcNAcbeta1-3Galbeta1-4Glc

GDP-beta-L-fucose + GalNAcbeta1-4GlcNAcbeta1-3Galbeta1-4Glc

GDP + GalNAcbeta1-4(Fucalpha1-3)GlcNAcbeta1-3Galbeta1-4Glc

Parabacteroides goldsteinii

GDP-beta-L-fucose + lacto-N-neotetraose

GDP + lacto-N-fucopentaose III

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778120

Substrates: -
Products: -

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GDP-beta-L-fucose + lactose

GDP + alpha1,3-fucosyllactose

Substrates: -
Products: -

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GDP-beta-L-fucose + N-acetyl-D-lactosamine

GDP + L-Fuc-alpha-(1->3)-[D-Gal-beta(1->4)]-D-GlcNac

GDP-beta-L-fucose + N-acetyllactosamine

GDP + ?

-

Substrates: -
Products: -

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GDP-beta-L-fucose + N-acetyllactosamine

GDP + L-Fuc-alpha-(1->3)-[D-Gal-beta(1->4)]-D-GlcNac

GDP-beta-L-fucose + Neu5Ac alpha(2,3)-Galbeta(1,4)-6-O-sulfo-GlcNAc beta-O-C3H6-biotin

?

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Substrates: i.e. 6-sulfo-3'SLN, best substrate
Products: -

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GDP-beta-L-fucose + Neu5Ac alpha(2,3)-Galbeta(1,4)-GlcNAc beta-(1,3)-Galbeta-(1,4)-Glcbeta-O-C3H6-biotin

?

-

Substrates: i.e. 3'SLNL
Products: -

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GDP-beta-L-fucose + Neu5Ac alpha(2,3)-Galbeta(1,4)-GlcNAc beta-O-BSA-biotin

?

-

Substrates: i.e. 3'SLN-BSA
Products: -

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GDP-beta-L-fucose + Neu5Ac alpha(2,3)-Galbeta(1,4)-GlcNAc beta-O-C3H6-biotin

?

-

Substrates: i.e. 3'SLN
Products: -

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GDP-beta-L-fucose + Neu5Acalpha-(2,3)-Galbeta-(1,4)-GlcNAcbeta-R

GDP + Neu5Acalpha-(2,3)-Galbeta-(1,4)-[Fucalpha-(1,3)]-GlcNAcbeta-R

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Substrates: the last step in sLex epitope formation is catalyzed byalpha(1,3)-fucosyltransferase VII, i.e. FucTVII, which transfers a fucosyl moiety from GDP-fucose to the core oligosaccharide structures, overview
Products: i.e. antigen sLex

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GDP-beta-L-fucose + NeuAcalpha(2,3)Galbeta(1,4)Glc

?

-

Substrates: -
Products: -

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GDP-beta-L-fucose + NeuAcalpha2,3Galbeta1,4GlcNAc

?

-

Substrates: 32% of the activity with Galbeta1,4GlcNAc
Products: -

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GDP-beta-L-fucose + NeuNAcalpha(2->3)Galbeta(1->4)GlcNAc

GDP + NeuNAcalpha(2->3)Galbeta(1->4)[L-Fucalpha(1->3)]GlcNAc

A1XCQ0

Substrates: best substrate
Products: -

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GDP-beta-L-fucose + octyl Galbeta(1->4)GlcNAc

GDP + octyl Galbeta(1->4)[L-Fucalpha(1->3)]GlcNAc

A1XCQ0

Substrates: -
Products: -

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GDP-beta-L-fucose + unsialylated biantennary glycan

?

-

Substrates: -
Products: -

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GDP-beta-L-galactopyranose + 2-azidoethyl 2-(acetylamino)-2-deoxy-4-O-beta-D-galactopyranosyl-beta-D-glucopyranoside

GDP + 2-azidoethyl beta-L-galactopyranosyl-(1->3)-[beta-D-galactopyranosyl-(1->4)]-2-(acetylamino)-2-deoxy-beta-D-glucopyranoside

Substrates: -
Products: -

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GDP-fucose + (-3GalNAcbeta1-4GlcNAcbeta1-)n

GDP + (-3GalNAcbeta1-4(Fucalpha1-3)GlcNAcbeta1-)n

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Substrates: -
Products: N-glycans containing poly-LDN structures are efficiently fucosylated by human FucT9 producing poly-LDNF structures, max. 4-6 repeating LDNF units

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GDP-fucose + (Galbeta1-4Galbeta1-4GlcNAcbeta1-2Manalpha1-6[Galbeta1-4Galbeta1-4(Fucalpha1-3)GlcNAcbeta1-2Manalpha1-3])Manbeta1-4GlcNAc

GDP + (Galbeta1-4Galbeta1-4(Fucalpha1-3)GlcNAcbeta1-2Manalpha1-6[Galbeta1-4Galbeta1-4(Fucalpha1-3)GlcNAcbeta1-2Manalpha1-3])Manbeta1-4GlcNAc

Danio rerio

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687445

Substrates: oligosaccharides are determined by a combination of exoglycosidase digestions and two-dimensional HPLC sugar mapping, most of these oligosaccharides are free form
Products: -

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GDP-fucose + (Galbeta1-4Galbeta1-4GlcNAcbeta1-2Manalpha1-6[Galbeta1-4Galbeta1-4(Fucalpha1-3)GlcNAcbeta1-2Manalpha1-3])Manbeta1-4GlcNAcbeta1-4GlcNAc

GDP + (Galbeta1-4Galbeta1-4(Fucalpha1-3)GlcNAcbeta1-2Manalpha1-6[GalbetaGalbeta1-4(Fucalpha1-3)GlcNAcbeta1-2Manalpha1-3])Manbeta1-4GlcNAcbeta1-4GlcNAc

Danio rerio

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687445

Substrates: -
Products: -

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GDP-fucose + asialo human erythropoietin

GDP + fucosylated asialo human erythropoietin

-

Substrates: -
Products: -

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GDP-fucose + bovine asialofetuin

GDP + fucosylated bovine asialofetuin

-

Substrates: -
Products: -

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GDP-fucose + Fucalpha1-2Galbeta1-4GlcNAc

GDP + Fucalpha1-2Galbeta1-4(Fucalpha1-3)GlcNAc

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349722, 349723

Substrates: -
Products: -

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GDP-fucose + Fucalpha1-2Galbeta1-4GlcNAc-O-(CH2)3NHCO(CH2)5-NH-biotin

GDP + Fucalpha1-2Galbeta1-4(Fucalpha1-3)GlcNAc-O-(CH2)3NHCO(CH2)5-biotin

-

Substrates: -
Products: -

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GDP-fucose + Fucalpha1-2Galbeta1-4GlcNAcbeta1-R

GDP + Fucalpha1-2Galbeta1-4(Fucalpha1-3)GlcNAcbeta1-R

687115

Substrates: -
Products: Lewis Y antigen, increased expression in cancer cells

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GDP-fucose + Galbeta(1,3)GlcNAcOMe

GDP + Galbeta(1,4)(Fucalpha(1,3))GlcNAcOMe

-

Substrates: -
Products: -

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GDP-fucose + Galbeta1-4Glc

GDP + Galbeta1-4 (Fucalpha1-3)Glc

-

Substrates: -
Products: -

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GDP-fucose + Galbeta1-4Glc-NAc-O-(CH2)3NHCO(CH2)5-NH-biotin

GDP + Galbeta1-4(Fucalpha1-3)GlcNAc-O-(CH2)3NHCO(CH2)5-NH-biotin

-

Substrates: -
Products: -

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GDP-fucose + Galbeta1-4GlcNAc

GDP + Galbeta1-4 (Fucalpha1-3)GlcNAc

-

Substrates: -
Products: -

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GDP-fucose + Galbeta1-4GlcNAc-O-(CH2)3NHCO(CH2)5-NH-biotin

GDP + Galbeta1-4(Fucalpha1-3)GlcNAc-O-(CH2)3NHCO(CH2)5-NH-biotin

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Substrates: sFUT9 efficiently fucosylates type II acceptors but not the corresponding sialylated acceptors, and only very poorly the type I (Galbeta3GlcNAc-R) related acceptors
Products: -

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GDP-fucose + Galbeta1-4GlcNAc-R

GDP + Galbeta1-4(Fucalpha1-3)GlcNAc-R

GDP-fucose + Galbeta1-4GlcNAcbeta1-2Manalpha1-6(Galbeta1-4GlcNAcbeta1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-Asn

GDP + Galbeta1-4(Fucalpha1-3)GlcNAcbeta1-2Manalpha1-6(Galbeta1-4(Fucalpha1-3)GlcNAcbeta1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-Asn

Substrates: -
Products: -

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GDP-fucose + Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glc-PA

GDP + Galbeta1-4(Fucalpha1-3)GlcNAcbeta1-3Galbeta1-4Glc-PA

Substrates: Lacto-N-neo-tetraose is a Lewis-type FucT substrate
Products: -

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GDP-fucose + GalNAcbeta1-4GlcNAcbeta-R

GDP + GalNAcbeta1-4(Fucalpha1-3)GlcNAcbeta1-R

-

Substrates: -
Products: -

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GDP-fucose + GalNAcbeta1-4GlcNAcbeta1-2Manalpha1-6(GalNAcbeta1-4GlcNAcbeta1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-Asn

GDP + GalNAcbeta1-4(Fucalpha1-3)GlcNAcbeta1-2Manalpha1-6(GalNAcbeta1-4(Fucalpha1-3)GlcNAcbeta1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-Asn

Substrates: -
Products: -

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GDP-fucose + GalNAcbeta1-4GlcNAcbeta1-3Galbeta1-4Glc

GDP + GalNAcbeta1-4[Fucalpha1-3]GlcNAcbeta1-3Galbeta1-4Glc

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349727

Substrates: incubation with extracts from transfected COS7 cells
Products: product obtained at a level of about 50% of that obtained with the acceptor GalNAcbeta1-4GlcNAcbeta1-R

?

GDP-fucose + GalNAcbeta1-4GlcNAcbeta1-R

GDP + GalNAcbeta1-4[Fucalpha1-3]GlcNAcbeta1-R

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349727

Substrates: -
Products: -

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GDP-fucose + human erythropoietin

GDP + fucosylated human erythropoietin

-

Substrates: -
Products: -

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GDP-fucose + LacNAc

GDP + ?

-

Substrates: -
Products: -

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GDP-fucose + LacNAcbeta-O-(CH2)5CO2CH3

GDP + ?

-

Substrates: -
Products: -

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GDP-fucose + lacto-N-neotetraose

GDP + ?

-

Substrates: -
Products: -

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GDP-fucose + lacto-N-neotetraose

GDP + lacto-N-fucopentaose III

GDP-fucose + methyl beta-D-2-O-methylgalactosyl-(1-4)-2-deoxy-2-acetylamino-6-deoxy-6-formylamino-beta-D-glucopyranose

GDP + methyl beta-D-2-O-methylgalactosyl-(1-4)-[alpha-D-fucosyl-(1-3)]-2-deoxy-2-acetylamino-6-deoxy-6-formylamino-beta-D-glucopyranose

-

Substrates: -
Products: -

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GDP-fucose + methyl beta-D-2-O-methylgalactosyl-(1-4)-6-O-methyl-N-acetyl-beta-D-glucosamine

GDP + methyl beta-D-2-O-methylgalactosyl-(1-4)-[alpha-D-fucosyl-(1-3)]-6-O-methyl-N-acetyl-beta-D-glucosamine

-

Substrates: -
Products: -

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GDP-fucose + methyl beta-D-2-O-methylgalactosyl-(1-4)-N-acetyl-beta-D-glucosamine

GDP + methyl beta-D-2-O-methylgalactosyl-(1-4)-[alpha-D-fucosyl-(1-3)]-N-acetyl-beta-D-glucosamine

-

Substrates: -
Products: -

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GDP-fucose + methyl beta-D-galactosyl-(1-4)-2-deoxy-2-acetylamino-6-deoxy-6-acetylamino-beta-D-glucopyranose

GDP + methyl beta-D-galactosyl-(1-4)-[alpha-D-fucosyl-(1-3)]-2-deoxy-2-acetylamino-6-deoxy-6-acetylamino-beta-D-glucopyranose

-

Substrates: -
Products: -

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GDP-fucose + methyl beta-D-galactosyl-(1-4)-2-deoxy-2-acetylamino-6-deoxy-6-amino-beta-D-glucopyranose

GDP + methyl beta-D-galactosyl-(1-4)-[alpha-D-fucosyl-(1-3)]-2-deoxy-2-acetylamino-6-deoxy-6-amino-beta-D-glucopyranose

-

Substrates: -
Products: -

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GDP-fucose + methyl beta-D-galactosyl-(1-4)-2-deoxy-2-acetylamino-6-deoxy-6-formylamino-beta-D-glucopyranose

GDP + methyl beta-D-galactosyl-(1-4)-[alpha-D-fucosyl-(1-3)]-2-deoxy-2-acetylamino-6-deoxy-6-formylamino-beta-D-glucopyranose

-

Substrates: -
Products: -

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GDP-fucose + methyl beta-D-galactosyl-(1-4)-2-deoxy-2-acetylamino-6-deoxy-6-methylsulfonylamino-beta-D-glucopyranose

GDP + methyl beta-D-galactosyl-(1-4)-[alpha-D-fucosyl-(1-3)]-2-deoxy-2-acetylamino-6-deoxy-6-methylsulfonylamino-beta-D-glucopyranose

-

Substrates: -
Products: -

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GDP-fucose + methyl beta-D-galactosyl-(1-4)-2-deoxy-2-acetylamino-beta-D-glucopyranosiduronic acid methyl amide

GDP + methyl beta-D-galactosyl-(1-4)-[alpha-D-fucosyl-(1-3)]-2-deoxy-2-acetylamino-beta-D-glucopyranosiduronic acid methyl amide

-

Substrates: -
Products: -

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GDP-fucose + methyl beta-D-galactosyl-(1-4)-2-deoxy-2-acetylamino-beta-D-glucopyranosiduronic acid methyl ester

GDP + methyl beta-D-galactosyl-(1-4)-[alpha-D-fucosyl-(1-3)]-2-deoxy-2-acetylamino-beta-D-glucopyranosiduronic acid methyl ester

-

Substrates: -
Products: -

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GDP-fucose + methyl beta-D-galactosyl-(1-4)-6-O-methyl-N-acetyl-beta-D-glucosamine

GDP + methyl beta-D-galactosyl-(1-4)-[alpha-D-fucosyl-(1-3)]-6-O-methyl-N-acetyl-beta-D-glucosamine

-

Substrates: -
Products: -

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GDP-fucose + methyl beta-D-galactosyl-(1-4)-6-O-methylsulfonyl-N-acetyl-beta-D-glucosamine

GDP + methyl beta-D-galactosyl-(1-4)-[alpha-D-fucosyl-(1-3)]-6-O-methylsulfonyl-N-acetyl-beta-D-glucosamine

-

Substrates: -
Products: -

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GDP-fucose + NeuAcalpha2-3Galbeta1-4GlcNAc

GDP + NeuAcalpha2-3Galbeta1-4(Fucalpha1-3)GlcNAc

GDP-fucose + sialyl-alpha-2,3-N-acetyllactosamine

GDP + NeuNAcalpha2-3Galbeta1-4[Fucalpha1-3]GlcNAc

-

Substrates: -
Products: -

?

GDP-L-fucose + 2'-fucosyllactose

GDP + ?

Substrates: 42% of the activity with Galbeta(1,4)GlcNAc
Products: -

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GDP-L-fucose + 2-azidoethyl 2-(acetylamino)-2-deoxy-4-O-beta-D-galactopyranosyl-beta-D-glucopyranoside

GDP + 2-azidoethyl 6-deoxy-beta-L-galactopyranosyl-(1-3)-[beta-D-galactopyranosyl-(1-4)]-2-(acetylamino)-2-deoxy-beta-D-glucopyranoside

Substrates: -
Products: -

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GDP-L-fucose + 3'-sialyl N-acetyl lactosamine

GDP + ?

-

Substrates: -
Products: -

?

GDP-L-fucose + alpha(2,3)-sialyllactosamine

GDP + ?

-

Substrates: 115% of the activity with Galbeta(1,4)GlcNAc
Products: -

?

GDP-L-fucose + alpha(2,3)sialyllactosamine

GDP + ?

Substrates: -
Products: -

?

GDP-L-fucose + alpha1 acid glycoprotein

GDP + ?

-

Substrates: with the major terminal structure on N-linked chains: NeuAc(2,3/6)Galbeta(1,4)GlcNAcbeta-R
Products: -

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GDP-L-fucose + asialo-alpha1-acid glycoprotein

GDP + ?

-

Substrates: with the major terminal structure on N-linked chains: Galbeta(1,4)GlcNAcbeta-R
Products: -

?

GDP-L-fucose + asialo-fetuin

GDP + ?

-

Substrates: with the major terminal structure on N-linked chains: Galbeta(1,4)GlcNAcbeta-R
Products: -

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GDP-L-fucose + asialotransferrin

GDP + ?

-

Substrates: -
Products: -

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GDP-L-fucose + desialylated alpha1-acid glycoprotein

GDP + ?

-

489362

Substrates: -
Products: -

?

GDP-L-fucose + fetuin

GDP + ?

GDP-L-fucose + fetuin

GDP + alpha1,3-L-fucosyl-fetuin

-

660698

Substrates: FucT-VI
Products: -

?

GDP-L-fucose + Fucalpha(1,2)Galbeta(1,3)GlcNAc-R

GDP + ?

GDP-L-fucose + Fucalpha(1,2)Galbeta(1,4)Glc

GDP + Fucalpha(1,2)Galbeta(1,4)(Fucalpha(1,3))Glc

GDP-L-fucose + Fucalpha(1,2)Galbeta(1,4)GlcNAc

GDP + Fucalpha(1,2)Galbeta(1,4)(Fucalpha(1,3))GlcNAc

GDP-L-fucose + Fucalpha(1,2)Galbeta(1,4)GlcNAcbeta(CH2)8COOMe

GDP + Fucalpha(1,2)Galbeta(1,4)(Fucalpha(1,3))GlcNAcbeta(CH2)8COOMe

-

639528

Substrates: -
Products: -

?

GDP-L-fucose + Fucalpha(1,2)Galbeta(1,4)GlcNAcbeta-bovine-serum-albumin

GDP + Fucalpha(1,2)Galbeta(1,4)(Fucalpha(1,3))GlcNAcbeta-bovine-serum-albumin

-

639526

Substrates: -
Products: -

?

GDP-L-fucose + Galalpha(1,3)Galbeta(1,4)GlcNAc-R

GDP + ?

GDP-L-fucose + Galbeta(1,3)GlcNAc

GDP + ?

Substrates: i.e. lacto-N-biose I, 10% of the activity with Galbeta(1,4)GlcNAc
Products: -

?

GDP-L-fucose + Galbeta(1,4)-Glc

GDP + Galbeta(1,4)(Fucalpha(1,3))Glc

-

Substrates: 3% of the activity with Galbeta(1,4)GlcNAc
Products: -

?

GDP-L-fucose + Galbeta(1,4)GlcNAc

GDP + Galbeta(1,4)(Fucalpha(1,3))GlcNAc

GDP-L-fucose + Galbeta(1,4)GlcNAc-O(CH2)8CO2CH3

GDP + Galbeta(1,4)(Fucalpha(1,3))GlcNAc-O(CH2)8CO2CH3

GDP-L-fucose + Galbeta(1,4)GlcNAc-R

GDP + Galbeta(1,4)(Fucalpha(1,3))GlcNAc-R

GDP-L-fucose + Galbeta(1,4)GlcNAcbeta(1,2)Manalpha(1,6)Manbeta(1,4)GlcNAc

GDP + Galbeta(1,4)(Fucalpha(1,3))GlcNAcbeta(1,2)Manalpha(1,6)Manbeta(1,4)GlcNAc

-

Substrates: -
Products: -

?

GDP-L-fucose + Galbeta(1,4)GlcNAcbeta(1,3)Galbeta(1,4)Glc

GDP + Galbeta(1,4)(Fucalpha(1,3))GlcNAcbeta(1,3)Galbeta(1,4)Glc

-

Substrates: -
Products: -

?

GDP-L-fucose + Galbeta(1,4)GlcNAcbeta(1,3)Galbeta(1,4)Glc

GDP + Galbeta(1,4)(Fucalpha(1,3))GlcNAcbeta(1,3)Galbeta(1,4)Glc + Galbeta(1,4)GlcNAcbeta(1,3)Galbeta(1,4)(Fucalpha(1,3))Glc

-

639517

Substrates: -
Products: -

?

GDP-L-fucose + lacto-N-biose I

GDP + ?

Substrates: -
Products: -

?

GDP-L-fucose + lacto-N-fucopentaose I

GDP + ?

-

Substrates: -
Products: -

?

GDP-L-fucose + lacto-N-fucopentaose II

GDP + ?

-

Substrates: -
Products: -

?

GDP-L-fucose + lacto-N-tetraose

GDP + ?

-

Substrates: -
Products: -

?

GDP-L-fucose + lactose

GDP + ?

GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine

GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-3)]-N-acetyl-beta-D-glucosaminyl]asparagine

-

349722, 349723

Substrates: -
Products: -

?

GDP-L-fucose + NeuAcalpha(2,3)Galbeta(1,4)GlcNAc

GDP + NeuAcalpha(2,3)Galbeta(1,4)(Fucalpha(1,3))GlcNAc

GDP-L-fucose + NeuAcalpha(2,3)Galbeta(1,4)GlcNAcbeta(1,2)Manalpha(1,6)Manbeta(1,4)GlcNAc

GDP + NeuAcalpha(2,3)Galbeta(1,4)(Fucalpha(1,3))GlcNAcbeta(1,2)Manalpha(1,6)Manbeta(1,4)GlcNAc

-

Substrates: -
Products: -

?

GDP-L-fucose + sialyl-alpha-2,3-LacNAc-O-(CH2)3-N-dansyl

GDP + Galbeta(1-4)-[Fucalpha(1-3)]GlcNAc-O-(CH2)3-N-dansyl

-

Substrates: -
Products: -

?

GDP-L-fucose + sialyl-alpha-2,3-N-acetyllactosamine

GDP + NeuNAcalpha2-3Galbeta1-4[Fucalpha1-3]GlcNAc

Q712G6

Substrates: -
Products: -

?

additional information

?

-

66 entries

GDP-beta-fucose + alpha2,3-sialyl N-acetyllactosaminyl-R

GDP + alpha1,3-fucosyl-alpha2,3-sialyl-N-acetyllactosaminyl-R

-

Substrates: FucT-VII and FucT-IV
Products: -

?

GDP-beta-L-fucose + asialo-erythropoietin

?

Q5Q0U2

Substrates: wild-type FUT9 efficiently fucosylates di-, tri- and tetraantennary N-glycans from asialoEPO
Products: -

?

GDP-beta-L-fucose + beta-D-galactosyl-(1->4)-N-acetyl-D-glucosaminyl-R

GDP + beta-D-galactosyl-(1->4)-[alpha-L-fucosyl-(1->3)]-N-acetyl-beta-D-glucosaminyl-R

-

Substrates: -
Products: -

?

GDP-beta-L-fucose + N-acetyllactosamine

GDP + L-Fuc-alpha-(1->3)-[D-Gal-beta(1->4)]-D-GlcNac

GDP-beta-L-fucose + Neu5Acalpha-(2,3)-Galbeta-(1,4)-GlcNAcbeta-R

GDP + Neu5Acalpha-(2,3)-Galbeta-(1,4)-[Fucalpha-(1,3)]-GlcNAcbeta-R

-

Substrates: the last step in sLex epitope formation is catalyzed byalpha(1,3)-fucosyltransferase VII, i.e. FucTVII, which transfers a fucosyl moiety from GDP-fucose to the core oligosaccharide structures, overview
Products: i.e. antigen sLex

?

additional information

?

-

31 entries

Ca2+

Co2+

-

alternative divalent metal cofactor

Mg2+

Mn2+

MnCl2

-

glycosyltransferase assay with 20 mM MnCl2

673753, 659454

Zn2+

additional information

1-ethynylnaphthalene

-

exhibits highly potent inhibitory effects against the FUT-VI mediated sialyl Lewis X synthesis

6-F-GDP-fucose

-

complete inhibition

Cu2+

deoxyfuconojirimycin

-

modest inhibitor, causes 21% inhibition, synergism: combined with GDP at their Ki levels results in 80% inhibition, combined with GMP causes 50.4% inhibition

diethyldicarbonate

dithionitrobenzene

-

GDP

GDP-(1-[2-[(naphthalen-1-ylmethyl)-carbamoyl]-ethyl]-1H-[1,2,3]triazol-4-ylmethyl)

-

GDP-alpha-D-glucose

-

GDP-alpha-D-mannose

-

GDP-choline

-

0.5 mM, 90% inhibition

GDP-fucose

GDP-hexanolamine

-

GDP-[1-[(1,2-diphenyl-ethylcarbamoyl)-methyl]-1H-[1,2,3]triazol-4-ylmethyl]

-

GDP-[1-[3-(1,2-diphenyl-ethylcarbamoyl)-butyl]-1H-[1,2,3]triazol-4-ylmethyl]

-

GDP-[1-[3-(1,2-diphenyl-ethylcarbamoyl)-propyl]-1H-[1,2,3]triazol-4-ylmethyl]

-

GDP-[1-[4-(9,10-dioxo-9,10-dihydro-anthracen-2-ylcarbamoyl)-butyl]-1H-[1,2,3]triazol-4-ylmethyl]

-

GMP

GTP

-

potent inhibitor

Inosine diphosphate

-

potent inhibitor

L-fucal

-

modest inhibitor, causes 26% inhibition, combined with GDP causes 40% inhibition

N-bromosuccinimide

-

moderate inhibition

639524

N-ethylmaleimide

NaCl

-

40% inhibition at 300 mM

NEM

Neu5(OHAc)alpha(2-3)Galbeta(1-4)GlcNAc-beta-(3-amino)propyl

-

Neu5Acalpha(2-3)Galbeta(1-4)6-deoxyGlcNAc-beta-methyl

-

Neu5Acalpha(2-3)Galbeta(1-4)6-sulfoGlcNAc-beta-methyl

-

Neu5Acalpha(2-3)Galbeta(1-4)GlcNAc-beta(1-3)LacN-(2-acetamido)ethyl

-

Neu5Acalpha(2-3)Galbeta(1-4)GlcNAc-beta(1-6)GalNAc-(2-acetamido)ethyl

-

Neu5Acalpha(2-3)Galbeta(1-4)GlcNAc-beta-(3-amino)propyl

-

Neu5Acalpha(2-3)Galbeta(1-4)GlcNAc-beta-biphenyl-4-carboxylic acid propyl amide

-

Neu5Acalpha(2-3)Galbeta(1-4)GlcNAc-beta-methyl

-

Ni2+

-

inhibits CEFT-4

686972

tunicamycin

Q712G6

inhibits N-glycosylation of the enzyme in recombinant COS-7 cells which leads to inactivation of rFuc-TVII

additional information

10 entries

-

beta-mercaptoethanol

-

additional information

-

FucT-IX-MBP fusion protein is soluble but catalytically inactive. From the culture supernatant of Sf9-insect cells, which constitutively produce recombinant FucT-IX, is obtained soluble protein with an enzymatic activity of approximately 250 mU/l after three days of cultivation, the level of activity differs depending on the type of signal sequence and the position of the tag. The melittin signal sequence and N-terminal tags result in the highest enzymatic activity

703540

-

0.65

3'-sialyl N-acetyl lactosamine

-

pH 7.2, 37°C

0.00094

6-deoxy-6-N-(2-naphthalene-2-yl-acetamide)-beta-L-galactopyranos-1-yl-guanosine 5'-diphosphate disodium salt

-

pH 7.5, 25°C

1.4

6-deoxy-alpha-L-galactopyranosyl-(1->2)-beta-D-galactopyranosyl-(1->3)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranose

-

pH 7.6, 15 mM MnCl2, 0.095 mM GDP-fucose

0.38

alpha(2,3) bisialylated biantennary glycan

-

pH 7.2, 37°C

-

7.8

alpha2,3-sialyl-N-acetyllactosamine

-

pH 6.5, 30°C, recombinant FucT-VII

6.7

Ca2+

-

1.2

Co2+

-

72 - 543

D-lactose

6.7 - 20

Fucalpha(1,2)Galbeta(1,4)Glc

0.7 - 3.9

Fucalpha(1,2)Galbeta(1,4)GlcNAc

1

Fucalpha1-2Galbeta1-4Glc

-

17

Gal-beta-(1->3)-[Fuc-alpha-(1->4)-])GlcNAc-beta-(1->3)-Gal-beta-(1->4)-Glc

-

pH 7.6, 15 mM MnCl2, 0.095 mM GDP-fucose

0.35

Galbeta(1,3)GlcNAcOMe

-

25

Galbeta(1,4)Glc

-

1.4 - 23

Galbeta(1,4)GlcNAc

1.5

Galbeta(1,4)GlcNAcbeta(1,3)Galbeta(1,4)Glc

-

pH 7.3, 37°C

0.37 - 1.7

Galbeta1,4GlcNAc-O(CH2)8CO2CH3

8.1

Galbeta1-4Glc

-

0.4

Galbeta1-4GlcNAc

-

0.021 - 6.448

GDP-beta-fucose

0.00012 - 1.12

GDP-beta-L-fucose

18 entries

0.0016 - 0.06

GDP-fucose

0.0055 - 0.062

GDP-L-fucose

8.8

LacNAc

-

0.17

LacNAcbeta-O-(CH2)5CO2CH3

-

0.3 - 0.76

lacto-N-neotetraose

5

lacto-N-tetraose

-

pH 7.6, 15 mM MnCl2, 0.095 mM GDP-fucose

3.729 - 83

lactose

0.075

methyl beta-D-2-O-methylgalactosyl-(1-4)-2-deoxy-2-acetylamino-6-deoxy-6-formylamino-beta-D-glucopyranose

-

0.12

methyl beta-D-2-O-methylgalactosyl-(1-4)-6-O-methyl-N-acetyl-beta-D-glucosamine

-

0.39

methyl beta-D-2-O-methylgalactosyl-(1-4)-N-acetyl-beta-D-glucosamine

-

0.19

methyl beta-D-galactosyl-(1-4)-2-deoxy-2-acetylamino-6-deoxy-6-acetylamino-beta-D-glucopyranose

-

0.4

methyl beta-D-galactosyl-(1-4)-2-deoxy-2-acetylamino-6-deoxy-6-amino-beta-D-glucopyranose

-

0.14

methyl beta-D-galactosyl-(1-4)-2-deoxy-2-acetylamino-6-deoxy-6-formylamino-beta-D-glucopyranose

-

0.115

methyl beta-D-galactosyl-(1-4)-2-deoxy-2-acetylamino-6-deoxy-6-methylsulfonylamino-beta-D-glucopyranose

-

1.54

methyl beta-D-galactosyl-(1-4)-2-deoxy-2-acetylamino-beta-D-glucopyranosiduronic acid methyl amide

-

0.29

methyl beta-D-galactosyl-(1-4)-6-O-methyl-N-acetyl-beta-D-glucosamine

-

0.25

methyl beta-D-galactosyl-(1-4)-6-O-methylsulfonyl-N-acetyl-beta-D-glucosamine

-

0.45

methyl beta-D-galactosyl-(1->4)-2-deoxy-2-acetylamino-beta-D-glucopyranosiduronic acid methyl ester

-

8.6

Mg2+

-

6.1

Mn2+

-

2.6

N-acetyl lactosamine

-

pH 7.2, 37°C

0.53

N-acetyl-beta-lactosamine

-

0.0026 - 0.61

N-acetyllactosamine

9 entries

0.05

Neu5Acalpha(2,3)-Galbeta(1,4)-6-O-sulfo-GlcNAc beta-O-C3H6-biotin

-

pH 7.4, 22°C

3.9

NeuAcalpha(2,3)Galbeta(1,4)Glc

-

0.2 - 0.9

NeuAcalpha(2,3)Galbeta(1,4)GlcNAc

0.96

NeuAcalpha2-3Galbeta1-4GlcNAc

-

1.7

sialyl-alpha-2,3-N-acetyllactosamine

-

fusion protein Hsp150delta-FucTe

0.74

unsialylated biantennary glycan

-

pH 7.2, 37°C

-

additional information

-

639521

-

2.25 - 5.97

D-lactose

1.7 - 3.6

Fucalpha(1,2)Galbeta(1,4)GlcNAc

1.1 - 2.3

Galbeta1,4GlcNAc-O(CH2)8CO2CH3

0.043 - 0.617

GDP-beta-fucose

0.063 - 2.3

GDP-beta-L-fucose

8 entries

0.02

lactose

pH 7.5, 37°C

0.042 - 0.367

N-acetyllactosamine

0.004 - 0.083

D-lactose

0.0079 - 0.096

GDP-beta-fucose

0.191 - 5.041

GDP-beta-L-fucose

0.0054

lactose

pH 7.5, 37°C

0.87

N-acetyllactosamine

pH 7.5, 37°C

0.0002

6-F-GDP-fucose

-

pH 7.4, 22°C

45

deoxyfuconojirimycin

-

0.0045 - 0.029

GDP

0.000901

GDP-(1-[2-[(naphthalen-1-ylmethyl)-carbamoyl]-ethyl]-1H-[1,2,3]triazol-4-ylmethyl)

-

0.38

GDP-alpha-D-glucose

-

0.29

GDP-alpha-D-mannose

-

0.0062 - 0.031

GDP-fucose

0.000437

GDP-[1-[(1,2-diphenyl-ethylcarbamoyl)-methyl]-1H-[1,2,3]triazol-4-ylmethyl]

-

0.000376

GDP-[1-[3-(1,2-diphenyl-ethylcarbamoyl)-butyl]-1H-[1,2,3]triazol-4-ylmethyl]

-

0.001069

GDP-[1-[3-(1,2-diphenyl-ethylcarbamoyl)-propyl]-1H-[1,2,3]triazol-4-ylmethyl]

-

0.000511

GDP-[1-[4-(9,10-dioxo-9,10-dihydro-anthracen-2-ylcarbamoyl)-butyl]-1H-[1,2,3]triazol-4-ylmethyl]

-

0.7

GMP

-

0.069

Inosine diphosphate

-

71

L-fucal

-

0.0054

1-ethynylnaphthalene

Homo sapiens

-

0.00064

6-F-GDP-fucose

Homo sapiens

-

pH 7.4, 22°C

0.092

diethyldicarbonate

Homo sapiens

-

IC50: 0.092 mM. Preincubation at 23°C gives maximal inhibition, 75%, after 180 s. Preincubation at 13°C gives 55% inhibition after 3 min. Preincubation at 4°C results in only 35% inhibition

639524

0.035

Neu5(OHAc)alpha(2-3)Galbeta(1-4)GlcNAc-beta-(3-amino)propyl

Homo sapiens

-

pH 7.4, 22°C

0.0279

Neu5Acalpha(2-3)Galbeta(1-4)6-deoxyGlcNAc-beta-methyl

Homo sapiens

-

pH 7.4, 22°C

0.0104

Neu5Acalpha(2-3)Galbeta(1-4)6-sulfoGlcNAc-beta-methyl

Homo sapiens

-

pH 7.4, 22°C

0.0838

Neu5Acalpha(2-3)Galbeta(1-4)GlcNAc-beta(1-3)LacN-(2-acetamido)ethyl

Homo sapiens

-

pH 7.4, 22°C

0.0347

Neu5Acalpha(2-3)Galbeta(1-4)GlcNAc-beta(1-6)GalNAc-(2-acetamido)ethyl

Homo sapiens

-

pH 7.4, 22°C

0.227

Neu5Acalpha(2-3)Galbeta(1-4)GlcNAc-beta-(3-amino)propyl

Homo sapiens

-

pH 7.4, 22°C

0.0143

Neu5Acalpha(2-3)Galbeta(1-4)GlcNAc-beta-biphenyl-4-carboxylic acid propyl amide

Homo sapiens

-

pH 7.4, 22°C

0.0516

Neu5Acalpha(2-3)Galbeta(1-4)GlcNAc-beta-methyl

Homo sapiens

-

pH 7.4, 22°C

0.000002

-

0.000017

substrate sialyl-N-acetyllactosamine

0.002289

-

0.067

-

mutant enzyme N101Q, at pH 4.5 and 37°C

0.154

-

mutant enzyme N62Q, at pH 4.5 and 37°C

0.248

-

wild type enzyme, at pH 4.5 and 37°C

1.4

-

additional information

5 - 7

5.5 - 6.5

-

recombinant FucT-VII

6

-

soluble enzyme

489362

6.2

-

membrane-bound enzyme

6.25

-

6.5

7

7 - 8

-

reaction with N-acetyllactosamine

7.2 - 8

-

7.4

-

assay at

7.5

7.6 - 8

-

8.5

maximal activity at pH 6.5 or 8.5

5 - 9.5

-

6 - 8.8

-

pH 6.0: about 40% of maximal activity, pH 8.8: about 50% of maximal activity, reaction with N-acetyllactosamine

6.5 - 9

pH range for FucTC is very broad

15

maximal activity

22

24

-

glycosyltransferase assay

25

-

assay at

30

Bacteroides fragilis NCTC 9343

-

assay at

37

assay at

4 - 50

temperature range for FucTC is very broad, enzyme shows 90% of maximal activity at 4°C and 40% of maximal activity at 50°C

-

SwissProt

honey-bee

SwissProt

Helicobacter pylori ATCC 43504

-

-

-

-

-

SwissProt

-

757573

J0U361

UniProt

Helicobacter pylori ATCC 700392

-

757573

O25142, O25366

UniProt

Helicobacter pylori NCTC11639

-

Helicobacter pylori UA948

-

-

-

-

-

-

-

-

-

-

-

-

-

-

SwissProt

Parabacteroides goldsteinii

-

-

-

Q2KQ71, Q70AG8, Q70AG9, Q70AH0, Q70AH1, Q70AH2, Q70AH3, Q70AH4

SwissProt

Tetraodon nigroviridis

-

UniProt

-

SwissProt

-

SwissProt

low metastatic cells

-

transcript of FUT6 mRNA detected

-

-

high metastatic cells

-

-

colorectal carcinoma cell

-

723313

commercial preparation

culture condition:CD4+ cell

-

FucT-VI

-

-

-

-

at the segmentation period, at 12, 18 and 48 h after fertilization

-

endothelial cell line

hepatocellular carcinoma cell

-

FUT7 mRNA minorly expressed in mouse brain endothelial cell line bEND.3

the enzyme is expressed in endothelial cells lining the high endothelial venules of perpheral lymph nodes, mesenteric lymph nodes and Pevers patches

P51993, P22083, Q9Y231

low expression

-

-

-

-

high endothelial venule

-

-

predominantly expressed

-

-

-

-

-

rFuc-TVII mRNA expression strongly detected in rat lymph node

lymphoid cell line

-

FUT7 mRNA remarkably highly expressed in mouse lymphoid tumor cell line TIB-63

667231

macrophage cell line

-

FUT7 mRNA significantly expressed in mouse macrophage cell line TIB-69

-

-

-

-

-

639519, 639520, 639521, 639531

-

-

CHP 134

-

-

-

-

-

-

-

-

-

-

-

-

predominantly expressed

-

additional information

cell wall

-

hsp150delta-FucTe fusion protein remains mostly non-covalently attached to the cell wall

-

-

-

-

-

-

-

granules of endothelial cells (Weibel-Parade bodies)

-

TI-VAMP, compartment of NT2N neurons

-

688548

Highest Expressing Human Cell Lines

Filter by:

Cell Line Links

Gene Links

drug target

evolution

Q9Y231

comparisons with other GT10 fucosyltransferases and GT-B fold glycosyltransferases provide evidence for modular evolution of donor- and acceptor-binding sites and specificity for Lewis antigen synthesis among mammalian GT10 fucosyltransferases

malfunction

metabolism

the enzyme is specifically responsible for Sialyl-Lewis X synthesis

physiological function

37800

-

after PNGaseF treatment

38000

Bacteroides fragilis

-

x * 38000, SDS-PAGE

735824

39000

39200

-

x * 39200, estimated from SDS-PAGE

723313

40680

-

x * 40680, SDS-PAGE

489362

41000

41300

-

SDS-PAGE, sFUT9

41892

Pan troglodytes

P56434, P56433

1 * 41892, calculation from nucleotide sequence

43008

43034

1 * 43034, calculation from nucleotide sequence

637649

44000

45000

-

gel filtration

47000

50556

1 * 50556, calculation from nucleotide sequence

51200

54000

x * 54000, glycosylated FUT9, SDS-PAGE, x * 47000, deglycosylated FUT9, SDS-PAGE

?

19 entries

monomer

9 entries

additional information

-

the enzyme possesses a cytoplasmic domain, a transmembrane domain, and a large Golgi lumenal catalytic domain

glycoprotein

8 entries

crystal structures of human FUT9, an alpha1,3-fucosyltransferase that generates Lewisx and Lewisy antigens, in complex with GDP, acceptor glycans, and as a FUT9-donor analog-acceptor Michaelis complex

K128D

R115W/E116D

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double mutation slightly increases H-type 1 activity

637646

A128N

the mutant displays 3.4fold higher catalytic activity with D-lactose than the wild type enzyme

A128N/H129E

the mutant displays 6.5fold higher catalytic activity with D-lactose than the wild type enzyme

A128N/H129E/S46F

14.5fold improvement in specific activity

A128N/H129E/Y132I

9.6fold improvement in specific activity

A128N/H129E/Y132I/S46F

15.5fold improvement in specific activity

E338A/C(DELTA)74

6% decrease in alpha1,3-fucosyllactose yield

E338D

a strain expressing FucTa-E338D achieves 2.57 g/l alpha1,3-fucosyllactose, a 41.4% higher yield than that of the strain carrying the wild-type enzyme FucTa

F49K/Y131D/Y197N/E338D/R369A

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best-performing mutant

G41E

150% increase in alpha1,3-fucosyllactose accumulation. The mutant exhibits an elevation in the production of byproducts

G41E/C(DELTA)74

52% decrease in alpha1,3-fucosyllactose yield

Y218K

the variant exhibits the highest alpha1,3-fucosyllactose yield, reaching 7.55 g/l in the shake flask growth experiment, which is 3.48-fold higher than that achieved by the wild-type enzyme. Subsequent fermentation optimization in a 5 l bioreactor resultsin a remarkable alpha1,3-fucosyllactose production of 36.98 g/l, highlighting the great prospects of the designed enzyme and the strains for industrial applications

Y218K/C(DELTA)74

67% decrease in alpha1,3-fucosyllactose yield

A349D

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mutant enzyme shows higher activity with a range of acceptor substrates, higher affinity for Fucalpha(1,2)Galbeta(1,4)GlcNAc, 8fold higher overall catalytic efficiency than that of wild-type enzyme. The single amino acid site Asp336 of FucT III and Ala349 of FucT V constitutes the only difference in the sequence of FucT III and V over the final 210 COOH-terminal amino acid residues, impacts the acceptor substrate profiles of FucT III and FuvT V

637645

C104S

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mutant enzyme is inactive, mutant enzyme produces a series of lower molecular weight bands when characterized by Wester blot and does not bind GDP

C351S

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mutant enzyme is inactive

C354S

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mutant enzyme is inactive

C64S

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FucT V mutant is secreted exclusively as monomer

680959

C94S

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mutant enzyme is inactive

N101Q

N101Q/N153Q

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the mutations lead to an almost complete loss of enzymatic activity

N153Q

N191Q/N153Q

Parabacteroides goldsteinii

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the mutant almost completely loses enzymatic activity

N62Q

N62Q/N101Q

N62Q/N153Q

N17D

-

the mutant is reactive toward lactose

778120

additional information

32 entries

FucTVII is very stable against freeze/thaw cycles

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lyophilized alpha-(1,3/1,4)-fucosyltransferase(1-428) loses 23% of its activity after 3 month

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lyophilized alpha-(1,3/1,4)-fucosyltransferase(1441) loses 38% activity after 3 months

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most stable at -80°C with or without glycerol supplementation

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affinity column chromatography

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alpha-(1,3/1,4)-fucosyltransferase(1428) with a His6 tag at the C terminus is purified

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native enzyme by preparation of microsome and Golgi membrane fractions, ultracentrifugation, and affinity chrotography

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695068

Ni-NTA column chromatography

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partial

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recombinant FucT-VII catalytic domain-protein A fusion protein from Sf21 insect cells by IgG affinity chromatography

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soluble enzyme

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using ammonium sulfate precipitation, hydrophobic chromatography in phenyl-Sepharose, ion-exchange chromatography on sulfopropyl-Sepharose, affinity chromatography on GDP-heanolamine-Sepharose, and finally high pressure liquid chromatography gel filtration

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using method A: column chromatography on DEAE-Sephadex, SP-Sephadex, ASTD-Sepharose and GTP-Agarose, method B: column chromatography on DEAE-Sephacel, SP-Sephadex and Synsorb-Galbeta1-4GlcNAc

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applying a baculovirus system in Spodoptera frugiperda Sf9 cells

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CEFT DNA and amino acid sequence determination and analysis, expression analysis, expression in COS-7 cells

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686972

cloning of rat Fuc-TVII gene which is expressed as two splice variants, but only the long one shows enzymatic activity, expression of the long splice variant in COS-7 cells

Q712G6

COS-7 cells transiently transfected with Fuc-TVII expression vectors

Q11131

639534

expressed in complex with green fluorescent protein in HEK cells

-

expressed in COS-1 cells

A1XCQ0

expressed in Escherichia coli BL21(DE3) cells

Bacteroides fragilis

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735824

expressed in Escherichia coli BW25113 (DE3) cells

expressed in Sf9 insect cells

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expression in Bacillus subtilis. A xylose-inducible bacteriophage T7 transcription system is transplanted into Bacillus subtilis 6051a to drive the heterologous expression of enzymes

expression in CHO cells

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expression in CHO cells, to confirm the hypothesis that FucT-VII and keratin sulfate sulfotransferase (KSST) may compete for the same acceptor molecules FucT-VII and Core2GlcNAcT-1 are expressed in CHO cells stably expressing KSST, sialyl Lewis X expression is significantly reduced in these cells

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673887

expression in COS cells

expression in Escherichia coli

expression in Escherichia coli. De novo biosynthesis of difucosyllactose by artificial pathway construction and alpha1,3/4-gucosyltransferase rational design in Escherichia coli. Engineering of Escherichia coli to endogenously overexpress genes manB, manC, gmd, and wcaG and heterologously overexpress a pair of fucosyltransferases to produce difucosyllactose from lactose. Introduction of alpha-1,2-fucosyltransferase from Helicobacter pylori (FucT2) along with alpha-1,3/4-fucosyltransferase (HP3/4FT)

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expression in hepatocellular carcinoma H7721 cells, alpha1,3FucT-VII acts as a potential antiapoptotic factor in H7721 cells after induction of apoptosis by UV irradiation, increasing phosphorylated JNK, quantitative real-time PCR analysis of expression of enzyme product SLex, overview

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692533

expression in Pichia pastoris

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expression in Pichia pastoris GS115 cells

expression in Saccharomyces cerevisiae

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expression in Sf9 cells

expression of FucTVII catalytic domain in HEK-293 cells

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FucT-VII-/-GFP+ bone marrow transplanted into lethally irradiated low-density lipoprotein receptor low density lipoprotein receptor mice or FucT-VII+/+GFP+ bone marrow into FucT-VII-/-, low density lipoprotein receptor double-mutant mice

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701615

FucT-VII: DNA and amino acid sequence determination and analysis, expression of the catalytic domain of FucT-VII fused to protein A from Staphylococcus aureus in Spodoptera frugiperda Sf21 insect cells via the baculovirus infection system

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full-length and each truncated FucT (DELTAFucT) are expressed in Escherichia coli HMS174DE3

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full-length form of human FUT9 and a truncated form containing sFUT9 the catalytic domain of the enzyme are overexpressed in Spodoptera frugiperda (Sf9) insect cells using the signal sequence of human interleukin 2 for efficient secretion, high activity of the trunctated, soluble form sFUT9 in the supernatant of Sf9 cells, sFUT9 fucosylates sialylated and non-sialylated glycoproteins

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fusion construct consisting of maltose-binding protein and soluble FucT-IX overexpressed in Escherichia coli. Constitutive expression of FucT-IX in Sf9 insect cells, different recombinant baculoviruses containing the N-terminal signal sequences from gp67and beta-trace proteins followed by a histidine tag fused to the soluble FucT-IX-encoding sequence, respectively, and infection of Sf9 cells

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703540

FUT9, DNA and amino acid sequence determination and analysis, transient overexpression of His-tagged or V5-tagged wild-type and mutant FUT9 proteins in HeLa cells

Q5Q0U2

gene fragment encoding the catalytic domain comprising residues 38359 of FUT9a fused N-terminally with the sequence encoding the first 53 aa of the Arabidopsis thaliana XylT gene. Hybrid gene placed under control of the Cassava Vein Mosaic virus promoter, and the resulting expression cassette combined with the one comprising the hybrid GalT under control of the CaMV 35S promoter. Transferred to a plant transformation vector conferring hygromycin resistance, which is designated xFxG

Tetraodon nigroviridis

Q6EV15

703055

into the vector pET-24b+ for expression in Escherichia coli cells

overexpression in A-431 cells cells, two stable FUT-4 cell lines A431-FUT4-1 and A431-FUT4-2

687115

poly-LDN backbone is efficiently fucosylated by recombinant human alpha1,3-fucosyltransferase IX (FucT 9) when it is stably co-expressed with the Cebeta4GalNAcT in cell line L8-GalNAcT-FucT

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putative cDNA amplified by PCR from a cDNAlambdaZAP library, cloned into the mammalian expression vector pCR3.1 and sequenced, transfection of COS7 kidney cells from the African green monkey with cDNA encoding the enzyme results in about 20fold level of activity over control cells, using lacto-N-neotetraose as acceptor

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349727

regions 5' of the FUT VI transcription start site, -2,067 to +1 nt and -2,067 to +213 nt isolated, PCR products 5'-phosphorylated using the T4 polynucleotide kinase and then ligated into pGL4.11 vector digested with EcoRV and treated with alkaline phosphatase from Escherichia coli. Plasmids transiently transfected into HepG2 and HuH-7 cells

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704786

the Apis mellifera genome encodes three alpha1,3-FucT homologues FucTA, FucTB and FucTC, expressed in yeast and insect cells only FucTA is found to be a core alpha1,3-FucT (EC 2.1.214), FucTC discloses to be the first Lewis-type alpha1,3-FucT (EC 2.4.1.152) to be described in insects, FucTB does not show any activity

transfection of COS-1 and CHO-T cells

P51993

639541

truncated constructs lacking the transmembrane region and the cytosolic N-terminus, are expressed in baculovirus-infected Trichoplusia ni insect cells and in two non-lytic expression systems, stably transfect human HEK 293 and Trichoplusia ni cells. Since secretion of some glycosyltransferases is controlled by formation of dimeric molecules via disulfide bonds, one of the fucosyltransferase V constructs contains the N-terminal cysteine residue 64 for dimerization, whereas this residue is replaced in the other construct by serine

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680959

breast cancer cell lines display increased expression of isoform FUT4

735901

decrease in FUT9 on hyperoxia exposure

FUT4 expression is increased in metastatic melanoma

777839

FUT4 mRNA is significantly upregulated during the early and midsecretory stages of the menstrual cycle compared with the other menstrual phases, with maximal expression during the mid-secretory phase. In proliferative explants, progesterone significantly increases FUT4 transcription and translation after 24 h in culture. Estrogen does not have any significant effects

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706697

FUT4 staining is weak in proliferative and menstrual endometrium. Inductive effect of progesterone on FUT4 transcription is lost after 48 h of treatment

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706697

higher mRNA and protein expression of the enzyme are observed in colorectal tumors compared with adjacent normal ones

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723313

in hepatocellular carcinoma tissues, the expression levels and activity of alpha1,3/4-fucosyltransferase FUT6 are significantly up-regulated

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721501

protein kinase R function is essential for FUT3, FUT5, and FUT6 induction and sLex expression in Herpes simplex virus type 1-infected cells. IMD-0354, an inhibitor of the NF-kappaB-activating factor IKK-2, induces FUT transcription via a IKK-2-independent mechanism, irrespective of whether the cells are virus-infected or not

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703902

protein kinase R inhibitors 2-aminopurine and C16 inhibit FUT3, FUT5, and FUT6 expression. MG-132 inhibits the IMD-0354-dependent transcription of FUT5 in a dose-dependent manner

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703902

the mRNA and protein expression levels of FUT7 are high in the MHCC-97 cell line compared with levels in normal liver cells

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756981

treatment with inflammatory factors interleukin-1beta pronouncedly upregulates alpha1,3-fucosyltransferase VII gene (FUT7) mRNA and protein expression level in EA.hy926 endothelial cells