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Literature summary for 2.4.1.152 extracted from

  • Stacke, C.; Ziegelmueller, P.; Hahn, U.
    Comparison of expression systems for human fucosyltransferase IX (2010), Eur. J. Cell Biol., 89, 35-38.
    View publication on PubMed
Search for more literature for 2.4.1.152

Activating Compound

Activating Compound Comment Organism Structure
additional information FucT-IX-MBP fusion protein is soluble but catalytically inactive. From the culture supernatant of Sf9-insect cells, which constitutively produce recombinant FucT-IX, is obtained soluble protein with an enzymatic activity of approximately 250 mU/l after three days of cultivation, the level of activity differs depending on the type of signal sequence and the position of the tag. The melittin signal sequence and N-terminal tags result in the highest enzymatic activity Homo sapiens

Application

Application Comment Organism
biotechnology a baculoviral expression system of FucT-IX appears to be a promising strategy for overproduction as compared to overproduction in Escherichia coli or mammalian cells Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
fusion construct consisting of maltose-binding protein and soluble FucT-IX overexpressed in Escherichia coli. Constitutive expression of FucT-IX in Sf9 insect cells, different recombinant baculoviruses containing the N-terminal signal sequences from gp67and beta-trace proteins followed by a histidine tag fused to the soluble FucT-IX-encoding sequence, respectively, and infection of Sf9 cells Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
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-
-

Synonyms

Synonyms Comment Organism
alpha1,3 fucosyltransferase
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 Homo sapiens
fucosyltransferaseIX
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 Homo sapiens
FucT-IX
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 Homo sapiens

General Information

General Information Comment Organism
physiological function largest amount of recombinant FucT-IX is obtained in Sf9-insect cells by use of a baculovirus inducible protein production system Homo sapiens