EC Number   |
|---|
   2.4.1.152 | - |
| 2.4.1.152 | affinity column chromatography |
| 2.4.1.152 | alpha-(1,3/1,4)-fucosyltransferase(1428) with a His6 tag at the C terminus is purified |
| 2.4.1.152 | native enzyme by preparation of microsome and Golgi membrane fractions, ultracentrifugation, and affinity chrotography |
| 2.4.1.152 | Ni-NTA column chromatography |
| 2.4.1.152 | partial |
| 2.4.1.152 | recombinant FucT-VII catalytic domain-protein A fusion protein from Sf21 insect cells by IgG affinity chromatography |
| 2.4.1.152 | soluble enzyme |
| 2.4.1.152 | using ammonium sulfate precipitation, hydrophobic chromatography in phenyl-Sepharose, ion-exchange chromatography on sulfopropyl-Sepharose, affinity chromatography on GDP-heanolamine-Sepharose, and finally high pressure liquid chromatography gel filtration |
| 2.4.1.152 | using method A: column chromatography on DEAE-Sephadex, SP-Sephadex, ASTD-Sepharose and GTP-Agarose, method B: column chromatography on DEAE-Sephacel, SP-Sephadex and Synsorb-Galbeta1-4GlcNAc |
