EC Number |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
---|
2.4.1.152 | 0.004 |
- |
D-lactose |
wild type enzyme, at pH 7.6 and 37°C |
735811 |
2.4.1.152 | 0.0054 |
- |
lactose |
pH 7.5, 37°C |
756354 |
2.4.1.152 | 0.0079 |
- |
GDP-beta-fucose |
cosubstrate lactose, pH 7.5, 37°C |
756354 |
2.4.1.152 | 0.012 |
- |
D-lactose |
mutant enzyme A128N, at pH 7.6 and 37°C |
735811 |
2.4.1.152 | 0.033 |
- |
D-lactose |
mutant enzyme A128N/H129E, at pH 7.6 and 37°C |
735811 |
2.4.1.152 | 0.041 |
- |
D-lactose |
mutant enzyme A128N/H129E/Y132I, at pH 7.6 and 37°C |
735811 |
2.4.1.152 | 0.069 |
- |
D-lactose |
mutant enzyme A128N/H129E/S46F, at pH 7.6 and 37°C |
735811 |
2.4.1.152 | 0.083 |
- |
D-lactose |
mutant enzyme A128N/H129E/Y132I/S46F, at pH 7.6 and 37°C |
735811 |
2.4.1.152 | 0.096 |
- |
GDP-beta-fucose |
cosubstrate N-acetyllactosamine, pH 7.5, 37°C |
756354 |
2.4.1.152 | 0.191 |
- |
GDP-beta-L-fucose |
wild type enzyme, at pH 7.6 and 37°C |
735811 |