EC Number   |
Protein Variants |
Reference |
|---|
   2.4.1.152 | A128N |
mutant obtained by focused directed evolution mutant, 3.4fold higher catalytic activity than wild-type |
-, 756241 |
| 2.4.1.152 | A128N |
the mutant displays 3.4fold higher catalytic activity with D-lactose than the wild type enzyme |
735811 |
| 2.4.1.152 | A128N/H129E |
the mutant displays 6.5fold higher catalytic activity with D-lactose than the wild type enzyme |
735811 |
| 2.4.1.152 | A128N/H129E/S46F |
14.5fold improvement in specific activity |
-, 756241 |
| 2.4.1.152 | A128N/H129E/Y132I |
9.6fold improvement in specific activity |
-, 756241 |
| 2.4.1.152 | A128N/H129E/Y132I/S46F |
15.5fold improvement in specific activity |
-, 756241 |
| 2.4.1.152 | A349D |
mutant enzyme shows higher activity with a range of acceptor substrates, higher affinity for Fucalpha(1,2)Galbeta(1,4)GlcNAc, 8fold higher overall catalytic efficiency than that of wild-type enzyme. The single amino acid site Asp336 of FucT III and Ala349 of FucT V constitutes the only difference in the sequence of FucT III and V over the final 210 COOH-terminal amino acid residues, impacts the acceptor substrate profiles of FucT III and FuvT V |
637645 |
| 2.4.1.152 | C104S |
mutant enzyme is inactive, mutant enzyme produces a series of lower molecular weight bands when characterized by Wester blot and does not bind GDP |
637641 |
| 2.4.1.152 | C351S |
mutant enzyme is inactive |
637641 |
| 2.4.1.152 | C354S |
mutant enzyme is inactive |
637641 |
