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Information on EC 3.4.24.B11 - ADAMTS1 endopeptidase
for references in articles please use BRENDA:EC3.4.24.B11
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preliminary BRENDA-supplied EC number
EC Tree 3 Hydrolases
3.4 Acting on peptide bonds (peptidases)
3.4.24 Metalloendopeptidases
3.4.24.B11 ADAMTS1 endopeptidase
The expected taxonomic range for this enzyme is: Tetrapoda
- 3.4.24.B11
- endothelial
-
antiangiogenic
- vessel
- metastasis
-
angiogenic
- endostatin
- vein
-
umbilical
- neovascularization
- huvecs
-
microvessels
- collagen
-
bevacizumab
-
nude
-
anti-angiogenesis
- thrombospondin-1
-
chorioallantoic
- colorectal
-
disintegrin
- capillary
-
brain-specific
-
microvascular
- plasminogen
-
vasculature
- metalloproteinases
- matrigel
-
progression-free
- angiostatin
-
vegf-induced
- fumagillin
- bfgf
- adamts4
-
avastin
-
angiogenesis-related
-
epithelium-derived
-
proangiogenic
- xviii
-
angiogenesis-dependent
- medicine
-
anti-vascular
- sorafenib
- sunitinib
- versican
- adam17
-
kringle
-
capillary-like
-
vegf-stimulated
- aggrecanase
- vegfr-2
- pazopanib
-
anti-vegf
showSynonyms
angiogenesis inhibitor, adamts1, adam metallopeptidase, meth-1, meth1, xadamts1, adam-ts1, xenopus ortholog of adamts1, adam-metallopeptidase with thrombospondin type 1 motifs-1, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
a disintegrin and metalloproteinase with thrombospondin motifs 1
a disintegrin-like and metalloproteinase domain with thrombospondin type I motifs-1
-
ADAM-TS 1
ADAM-TS1
ADAMTS-1
ADAMTS1
disintegrin-like and metalloproteinase with thrombospondin type 1 motifs
-
M12.222
METH-1
a disintegrin and metalloproteinase with thrombospondin motifs 1
-
-
a disintegrin and metalloproteinase with thrombospondin motifs 1
-
-
ADAMTS-1
-
a matrix metalloproteinase with disintegrin and thrombospondin motifs
ADAMTS-1
-
disintegrin-like and metalloproteinase with thrombospondin type I motifs-1
ADAMTS-1
a disintegrin and metalloproteinase with thrombospondin motifs-1
ADAMTS-1
-
a disintegrin and metalloproteinase with thrombospondin motifs
ADAMTS1
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
proteolytic degradation of proteins
strict specificity for cleavage between glutamic acid and uncharged aliphatic amino acids in the core proteins of large aggregating proteoglycans
proteolytic degradation of proteins
enzyme has multiple and non-redundant functions in organogenesis, enzyme inhibits endothelial cell proliferation
proteolytic degradation of proteins
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of peptide bond
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CAS REGISTRY NUMBER
COMMENTARY
241475-68-3
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
aggrecan + H2O
11 aggrecan fragment
Substrates: several cleavage sites are determined
Products: product determination, overview
Products: product determination, overview
?
aggrecan + H2O
2 aggrecan fragment
-
Substrates: cleavage site is Glu373-Ala374
Products: -
Products: -
?
alpha2-macroglobulin + H2O
?
-
Substrates: cleavage of the bait region of alpha2-macroglobulin, complex formation with alpha2-macroglobulin is dependent on the proteolytic activity
Products: -
Products: -
?
biglycan + H2O
?
Substrates: biglycan is a small leucine-rich proteoglycan
Products: -
Products: -
?
fluorescein-5(6)-carbonyl-Ala-Glu-Leu-Asn-Gly-Arg-Pro-Ile-Ser-Ile-Ala-Lys + H2O
?
Substrates: -
Products: -
Products: -
?
peptide fragments of aggrecan + H2O
?
-
Substrates: peptide sequences contain the cleavage site of aggrecan at E373-A374
Products: -
Products: -
?
thrombospondin 1 + H2O
?
-
Substrates: cleavage site...TEENRELVSE/LKRPPL...
Products: -
Products: -
?
thrombospondin-1 + H2O
36000 Da fragment + 110000 Da fragment
-
Substrates: -
Products: -
Products: -
?
thrombospondin-2 + H2O
42000 Da fragment + 30000 Da fragment
-
Substrates: -
Products: -
Products: -
?
versican + H2O
DPEAAE + ?
-
Substrates: ADAMTS-1 cleaves versican at the Glu441-Ala442 bond to generate a 70000 Da fragment termed DPEAAE
Products: -
Products: -
?
versican isoform V0 + H2O
2 peptides
-
Substrates: a second cleavage site might be Glu405-Gln406, cleavage site is Glu1428-Ala1429 in the V0 isoform, activity with recombinant and native human substrate protein
Products: product identification
Products: product identification
?
versican isoform V1 + H2O
2 peptides
-
Substrates: a second cleavage site might be Glu405-Gln406, cleavage site is Glu441-Ala442 in the V1 isoform, activity with recombinant and native human substrate protein
Products: product identification
Products: product identification
?
aggrecan + H2O
?
Substrates: in 50 mM Tris-HCl, pH 7.6, 150 mM NaCl and 5mM CaCl2 solution with 100 ng recombinant ADAMTS-1 at 37°C
Products: -
Products: -
?
aggrecan + H2O
?
-
Substrates: C-terminal spacer region of the enzyme is required for activity, substrate is a cartilage proteoglycan, cleavage site is the peptide bond Glu1871-Leu1872 within the substrates chondroitin sulfate attachment domain
Products: -
Products: -
?
aggrecan + H2O
?
-
Substrates: substrate is a cartilage proteoglycan
Products: -
Products: -
?
brevican + H2O
?
-
Substrates: assay at 37°C, 3 h, 25 nM recombinant ADAMTS
Products: -
Products: -
?
proteins + H2O
peptides
Substrates: enzyme suppresses FGF2-induced vascularization in a cornea pocket assay and inhibited VEGF-induced angiogenesis in a chorioallantoic membrane assay
Products: -
Products: -
?
proteins + H2O
peptides
-
Substrates: rather restricted substrate specificity
Products: -
Products: -
?
proteins + H2O
peptides
Substrates: strict specificity for cleavage between glutamic acid and uncharged aliphatic amino acids in the core proteins of large aggregating proteoglycans
Products: -
Products: -
?
proteins + H2O
peptides
-
Substrates: enzyme is involved in the inflammatory process
Products: -
Products: -
?
syndecan-4 + H2O
?
Substrates: ADAMTS1 cleaves the ectodomain of syndecan-4. The shedding of syndecan-4 by ADAMTS1 disrupts cell adhesion and promotes cell migration
Products: -
Products: -
?
syndecan-4 + H2O
?
Substrates: ADAMTS1 cleaves the ectodomain of syndecan-4
Products: -
Products: -
?
versican + H2O
?
-
Substrates: chondroitin sulphate proteoglycan-2
Products: -
Products: -
?
versican + H2O
?
Substrates: ADAMTS1 versicanase activity is approximately 1000fold lower than ADAMTS5 and 50fold lower than ADAMTS4
Products: -
Products: -
?
versican + H2O
?
Substrates: versican proteolysis in cardiac jelly at the canonical Glu441-Ala442 site is cooperatively mediated by ADAMTS1 and ADAMTS5 and required for proper ventricular cardiomyogenesis
Products: -
Products: -
?
additional information
?
-
Substrates: enzyme inhibits fibroblast growth factor 2-induced vascularization in the cornea pocket assay and inhibits vascular endothelial growth factor-induced angiogenesis in the chorioallantoic membrane assay
Products: -
Products: -
?
additional information
?
-
-
Substrates: enzyme inhibits fibroblast growth factor 2-induced vascularization in the cornea pocket assay and inhibits vascular endothelial growth factor-induced angiogenesis in the chorioallantoic membrane assay
Products: -
Products: -
?
additional information
?
-
-
Substrates: no activity with pro-collagen type I and III, collagen types I, II, IV, V, VIII and XVIII, fibronectin, laminin, vibronectin, and thrombospondin 1
Products: -
Products: -
?
additional information
?
-
-
Substrates: vascular endothelial growth factor 165 and vascular endothelial growth factor 165 receptor tyrosine kinase are no substrates for proteolytic cleavage, but the enzyme blocks endothelial vascularization by inhibiting the necessary phosphorylation of the receptor
Products: -
Products: -
?
additional information
?
-
-
Substrates: vascular endothelial growth factor 165 and vascular endothelial growth factor 165 receptor tyrosine kinase are no substrates for proteolytic cleavage, but the enzyme blocks endothelial vascularization by inhibiting the necessary phosphorylation of the receptor
Products: -
Products: -
?
additional information
?
-
-
Substrates: enzyme has anti-aniogenic properties, processed form p65 shows altered affinity to heparin and endothelial cells, reduced activity upon suppression of endothelial cell proliferation
Products: -
Products: -
?
additional information
?
-
Substrates: enzyme inhibits endothelial cell proliferation, but not fibroblast or smooth muscle growth
Products: -
Products: -
?
additional information
?
-
-
Substrates: enzyme inhibits endothelial cell proliferation, but not fibroblast or smooth muscle growth
Products: -
Products: -
?
additional information
?
-
Substrates: enzyme is genetically regulated by progesteron in the ovulation process
Products: -
Products: -
?
additional information
?
-
-
Substrates: interaction with heparin via the first TSP1 domain at the C-terminal part, enzyme shows an antiangiogenic effect, the enzyme is able to shed proteins from the surface of the endothelium
Products: -
Products: -
?
additional information
?
-
-
Substrates: vascular endothelial growth factor 165 and vascular endothelial growth factor 165 receptor tyrosine kinase are no substrates for proteolytic cleavage, but the enzyme blocks endothelial vascularization by inhibiting the necessary phosphorylation of the receptor, interaction with heparin via the first TSP1 domain at the C-terminal part, enzyme shows an antiangiogenic effect, the enzyme is able to shed proteins from the surface of the endothelium
Products: -
Products: -
?
additional information
?
-
-
Substrates: enzyme plays a role in cell-cell and cell-matrix interactions, in cell adhesion and growth factor signaling
Products: -
Products: -
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
syndecan-4 + H2O
?
Substrates: ADAMTS1 cleaves the ectodomain of syndecan-4. The shedding of syndecan-4 by ADAMTS1 disrupts cell adhesion and promotes cell migration
Products: -
Products: -
?
versican + H2O
DPEAAE + ?
-
Substrates: ADAMTS-1 cleaves versican at the Glu441-Ala442 bond to generate a 70000 Da fragment termed DPEAAE
Products: -
Products: -
?
aggrecan + H2O
?
-
Substrates: substrate is a cartilage proteoglycan
Products: -
Products: -
?
proteins + H2O
peptides
-
Substrates: enzyme is involved in the inflammatory process
Products: -
Products: -
?
versican + H2O
?
Substrates: versican proteolysis in cardiac jelly at the canonical Glu441-Ala442 site is cooperatively mediated by ADAMTS1 and ADAMTS5 and required for proper ventricular cardiomyogenesis
Products: -
Products: -
?
additional information
?
-
-
Substrates: enzyme has anti-aniogenic properties, processed form p65 shows altered affinity to heparin and endothelial cells, reduced activity upon suppression of endothelial cell proliferation
Products: -
Products: -
?
additional information
?
-
Substrates: enzyme inhibits endothelial cell proliferation, but not fibroblast or smooth muscle growth
Products: -
Products: -
?
additional information
?
-
-
Substrates: enzyme inhibits endothelial cell proliferation, but not fibroblast or smooth muscle growth
Products: -
Products: -
?
additional information
?
-
Substrates: enzyme is genetically regulated by progesteron in the ovulation process
Products: -
Products: -
?
additional information
?
-
-
Substrates: interaction with heparin via the first TSP1 domain at the C-terminal part, enzyme shows an antiangiogenic effect, the enzyme is able to shed proteins from the surface of the endothelium
Products: -
Products: -
?
additional information
?
-
-
Substrates: vascular endothelial growth factor 165 and vascular endothelial growth factor 165 receptor tyrosine kinase are no substrates for proteolytic cleavage, but the enzyme blocks endothelial vascularization by inhibiting the necessary phosphorylation of the receptor, interaction with heparin via the first TSP1 domain at the C-terminal part, enzyme shows an antiangiogenic effect, the enzyme is able to shed proteins from the surface of the endothelium
Products: -
Products: -
?
additional information
?
-
-
Substrates: enzyme plays a role in cell-cell and cell-matrix interactions, in cell adhesion and growth factor signaling
Products: -
Products: -
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Zn2+
additional information
Zn2+
-
metalloprotease, contains zinc binding motif HEXXHXXXXXHD in the metalloprotease domain
Zn2+
-
enzyme contains a Zn2+-binding peptidase consensus sequence HEXXH involved in catalysis
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
catechin gallate esters
from green tea Camellia sinensis, strong inhibition, independent of Zn2+, reversible
-
heparin
-
heparin inhibits cleavage of thrombonspondin-2, but not thrombonspondin-1
additional information
-
no inhibition by minocycline, and matrix metalloprotease inhibitors TIMP1 and TIMP4
-
additional information
-
no inhibition by leupeptin, aprotinin, phenylmethylsulfonyl floride, and pepstatin
-
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
fibulin-1
4fold enhancement of activity with 0.001 mg at 1:1, 1:2, and 1:3 molar ratio in 50 mM Tris, pH 7.4, 10 mM CaCl2, 80 mM NaCl overnight at 37 °C
-
additional information
higher enzyme expression in degenerated intervertebral discs
-
additional information
-
higher expression level in metastatic foci compared with corresponding primary tumor
-
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0135
fluorescein-5(6)-carbonyl-Ala-Glu-Leu-Asn-Gly-Arg-Pro-Ile-Ser-Ile-Ala-Lys
37°C, pH 7.45, wild-type enzyme
-
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.054
fluorescein-5(6)-carbonyl-Ala-Glu-Leu-Asn-Gly-Arg-Pro-Ile-Ser-Ile-Ala-Lys
37°C, pH 7.45, wild-type enzyme
-
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
42
fluorescein-5(6)-carbonyl-Ala-Glu-Leu-Asn-Gly-Arg-Pro-Ile-Ser-Ile-Ala-Lys
37°C, pH 7.45, wild-type enzyme
-
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7.6
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
37
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
brenda
enzyme belongs to the subfamily of adamalysins which is part of the superfamily of zinc-dependent proteases
-
-
brenda
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
ADAMTS1 expression is reduced in primary breast cancers compared to normal tissue
brenda
-
embryonic presumptive ectoderm, Spemann organizer region, trunk organizer region and posterior ectoderm
brenda
-
basal lamina of epithelium of intestine, nasal cavity, choroids plexus, skin and intracellular storage vesicles
brenda
ADAMTS1 is highly expressed in non-small cell lung cancer tissues
brenda
additional information
-
ADAMTS-1 is localized to developing mother vessel endothelial cells
brenda
down regulation in endothelial cells in cirrhotic rats
brenda
-
ADAMTS1 is transiently induced by hypoxia in endothelial cells, its transcription is mediated by hypoxia-inducible factor-1 binding
brenda
macrophages at sites of muscle injury induce activation of satellite cells via expression of Adamts1
brenda
additional information
-
intravenous administration of lipopolysaccharides resulted in inflammation which induced the enzyme expression exclusively in heart and kidney
brenda
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
ADAMTS-1 is present primarily in the nucleus and with a relatively homogeneous distribution in MCF-10A, MCF-7, and MDA-MB-231 cells.Substrate aggrecan is also found in the nuclei of MCF-10A cells, but not entirely colocalized with ADAMTS-1
brenda
-
the mature processed enzyme form is soluble, the unprocessed enzyme proform is associated with the extracellular matrix via the first TSP-1 repeat that can bind to heparin
-
brenda
Highest Expressing Human Cell Lines
Cell Line LinksPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
metabolism
physiological function
additional information
spacer and cysteine-rich domains as major determinants of ADAMTS1 versicanase activity. These C-terminal domains are involved in the proteolysis of aggrecan as well as biglycan, a small leucine-rich proteoglycan
malfunction
silencing of Adamts1 blocks the adverse effects of ethanol on collagen and elastin levels
malfunction
combined inactivation of ADAMTS1 and ADAMTS5 prior to 12.5 days of gestation leads to dramatic accumulation of versican-rich cardiac jelly and inhibits formation of compact and trabecular myocardium
metabolism
the enzyme is controlled by the FoxO1-sestrin 3-AMP-activated protein kinase signaling cascade and regulates ethanol-induced changes in collagen I and elastin levels
metabolism
a potential mechanism whereby ADAMTS1 promotes breast cancer metastasis is described
physiological function
-
roles in angiogenesis and tumor biology, important regulatory factor of angiogenesis and tumor growth in prostate tumors
physiological function
-
ADAMTS1 has an impact on bone mineral density and is a potent regulator of bone remodeling. Adamts1 induces parathyroid hormone levels in females and reduces osteocalcin levels in males
physiological function
the enzyme inhibits cell proliferation, migration, and tube formation. The enzyme also inhibits vascular endothelial growth factor receptor 3 signaling under vascular endothelial growth factor-C stimulation
physiological function
in Adamts1-deficient mice, alterations of organs like kidney, heart and aorta are found. In addition, the lack of ADAMTS1 differently affects lymphocyte and myeloid populations in the spleen and bone marrow. The substrate versican is altered the absence of the protease. the absence of ADAMTS1 creates a pro-inflammatory landscape, correlating with tumour blockade
physiological function
expression of ADAMTS1 increases in middle-aged and old wild-type mice compared with young, whereas in SPARC (i.e. secreted protein acidic and rich in cysteine) mice only old animals show increased ADAMTS1 expression. Levels of ADAMTS1 substrate versican decrease with age only in wild-type. SPARC treatment increases secretion of collagen I and ADAMTS1 (both the 110-kDa latent and 87-kDa active forms) into the conditioned media as well as the cellular expression of transforming growth factor-beta1-induced protein (Tgfbi) and phosphorylated Smad2
physiological function
transgenic mice overexpressing ADAMTS-1 are similar to ADAMTS-1 wild-type mice pertaining collagen, elastin content and aortic diameter
physiological function
macrophages at sites of muscle injury induce activation of satellite cells via expression of Adamts1. Overexpression of Adamts1 in macrophages in vivo is sufficient to increase satellite cell activation and improve muscle regeneration in young mice. NOTCH1 is a target of ADAMTS1 metalloproteinase activity, which reduces Notch signaling, leading to increased satellite cell activation
physiological function
-
HT-1080 cells overexpressing ADAMTS-1 exhibit a significant decrease in cell proliferation and migration velocity, both in presence of hepatocyte growth factor. ADAMTS-1 overexpression disturbs c-Met activation upon hepatocyte growth factor stimulation. Downstream ERK1/2 and FAK signaling pathways are also influenced. ADAMTS-1 decreases the size of the fibrosarcospheres, both under normal conditions and in the presence of hepatocyte growth factor. In presence of hepatocyte growth factor, ADAMTS-1 overexpression in HT-1080 disrupts microtumors formation in vivo
physiological function
ADAMTS1 functioned as an oncogene in NSCLC cells by promoting TGF-beta expression, indicating that ADAMTS1 has important regulatory roles in the progression of non-small cell lung cancer
physiological function
ADAMTS1 is required at early stages of human uveal melanoma development by inducing stemness and endothelial-like features on tumor cells
physiological function
ADAMTS1 promotes malignant invasion of glioma. ADAMTS1 may affect the invasive phenotype of glioma stem cells by regulating Notch1-SOX2 signaling pathway, thereby promoting the invasive growth of glioma
physiological function
ADAMTS1 is involved in fertilization, cancer, cardiovascular development, and thoracic aneurysms
physiological function
ADAMTS1 is a matrix-degrading metalloproteases. Versican proteolysis in cardiac jelly at the canonical Glu441-Ala442 site is cooperatively mediated by ADAMTS1 and ADAMTS5 and required for proper ventricular cardiomyogenesis
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). ATS1_MOUSE
968
0
105801
Swiss-Prot
Secretory Pathway (Reliability: 1)
Q8NE26_HUMAN
967
0
105388
TrEMBL
other Location (Reliability: 4)
ATS1_HUMAN
967
0
105358
Swiss-Prot
other Location (Reliability: 1)
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
110000
115000
67000
x * 67000, recombinant processed form 2, SDS-PAGE, x * 85000, recombinant processed form 1, SDS-PAGE, x * 110000, recombinant unprocessed enzyme, SDS-PAGE
85000
x * 67000, recombinant processed form 2, SDS-PAGE, x * 85000, recombinant processed form 1, SDS-PAGE, x * 110000, recombinant unprocessed enzyme, SDS-PAGE
87000
90000
110000
x * 67000, recombinant processed form 2, SDS-PAGE, x * 85000, recombinant processed form 1, SDS-PAGE, x * 110000, recombinant unprocessed enzyme, SDS-PAGE
87000
-
SDS-PAGE, variant of ADAMTS1 in the supernatant of both fibroblasts and keratinocytes
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
?
x * 67000, recombinant processed form 2, SDS-PAGE, x * 85000, recombinant processed form 1, SDS-PAGE, x * 110000, recombinant unprocessed enzyme, SDS-PAGE
additional information
enzyme is composed of a ADAM-like protease domain, a disintegrin-like, a thrombospondin type 1 repeat and a cysteine-rich domain, but no transmembrane domain
additional information
-
latent form 110000 Da, active form 87000 Da, Western blot analysis
additional information
-
enzyme is a multidomain protease possessing a prodomain, a cysteine-rich domain, an EGF-like domain, a transmembrane domain, and a cytoplasmic tail
additional information
-
enzyme is composed of a ADAM-like protease domain, a disintegrin-like, a thrombospondin type 1 repeat and a cysteine-rich domain, but no transmembrane domain
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POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
proteolytic modification
proteolytic modification
cleavage at the consensus subtilisin site RKKR resulting in 2 different processed forms of MW 85 kDa and 67 kDa
proteolytic modification
-
cleavage of prodomain by furin at furin-cleavage site RKKR
proteolytic modification
recombinant enzyme is also processed in insect cells
proteolytic modification
-
secreted pro-METH-1 is processed in 2 consecutive steps to release a p87 and a p65 active form, cleavage by furin results in only the p87 fragment, the p87 form lacks the N-terminal prodomain, and p65 results from an additional processing event in the C-terminus requiring p87 which can be catalyzed by matrix metalloproteases 2,8, and 15
proteolytic modification
-
enzyme is synthesized as an inactive proform, proteolytical processing of a conserved cysteine residue in its prodomain
proteolytic modification
-
cleavage of the proform by furin at the furin cleavage site
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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
in complex with marimastat, sitting drop vapour diffusion method with 0.2-0.6 M sodium acetate, 0.05 M cadmium sulfate, 0.1 M HEPES (pH 7.0), and 12-22% glycerol
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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
E385A
E386Q
additional information
E385A
-
point mutation in the zinc binding domain results in an inactive mutant enzyme form
E385A
-
point mutation in the zinc binding domain, catalytically inactive mutant
E386Q
-
oligonucleotide primer mutagenesis, mutation within the zinc binding motif, loss of zinc-binding capacity, loss of activity and capacity to complex with alpha2-macroglobulin
E386Q
-
oligonucleotide primer mutagenesis, mutation within the zinc binding motif, inactive
additional information
-
induction of tumor growth in nude mice by injection of carcinoma cells, cell line T47D expressing the recombinant His-Myc-tagged wild-type or mutant E385A enzyme, number and distribution of vessels in the tumors are reduced compared to control tumors, high degree of edema and necrosis, the truncated construct comprising the amino acid residues 549-951 had no effect on tumor growth, but the isolated first TSP1 repeat does
additional information
-
introduction of a KpnI site at the 5'-end, expression in carcinoma cell line T47D
additional information
knock out mutant mice exhibit significant growth retardation, impaired female fertility and adipose tissue malformation, as well as kidney structure changes
additional information
-
construction of soluble C-terminal deletion mutants, that are still able to form complexes with alpha2-macroglobulin
additional information
-
knock-out mutant shows a phenotype with reduced growth and body weight, as well as pathological changes in the kidney structure, obstructive fibrosis, disrupted capillary networks in the adrenal gland, and hypoplastic or atrophic papillae
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Hi-trap heparin-sepharose column chromatography and Nickel-chelating chromatography
Ni-NTA Superflow column chromatography, Ni-NTA resin column chromatography, and Superdex 75 gel filtration
Ni2+-probond and anti-v5 antibody-conjugated affinity column chromatography
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
adamts-1 gene maps to chromosome 16, region C3-C5, transient expression in COS-7 cells of FLAG-tagged wild-type and deletion mutants and mutant E386Q
-
DNA and amino acid sequence determination and analysis, in itro translation, expression as His-tagged protein in Escherichia coli strain BL21
expressed in High-FiveTM insect cells with pIB/V5-His insect cell expression vector
expression of full-length wild-type enzyme in human embryonic kidney cell line 293T, expression of C-terminal region of the enzyme, corresponding to amino acid residues 549-951, in breast tumor derived cell line T47D
-
expression of His-Myc-tagged wild-type enzyme and tagged mutant E385A, as well as truncated construct comprising the amino acid residues 549-951, in the carcinoma cell line T47D
-
expression of His-tagged enzyme in insect cells via baculovirus infection
transient expression in HEK293 cells of FLAG- and His-tagged wild-type and deletion mutants, the majority of the recombinant protein is expressed in an active form without the proprotein domain
-
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
ADAMTS-1 mRNA expression is strongly, indirectly and progressively upregulated over the period of 30 min to 16 h by vascular endothelial growth factor A
-
ADAMTS1 expression is reduced in primary breast cancers compared to normal tissue
ADAMTS1 is up-regulated in non-small cell lung cancer tumor tissues. ADAMTS1 mRNA and protein levels in non-small cell lung cancer tissues is significantly higher than those in adjacent normal tissues
elevated ADAMTS1 expression in endometrial adenocarcinoma, PGF2alpha-FP signalling upregulates ADAMTS1 expression via a calmodulin-NFAT-dependent pathway
-
incubation of myocytes with ethanol increases Adamt1 mRNA without changing the overall level of Adamts1 protein
luteinizing hormone/human chorionic gonadotropin (LH/hCG)-induced gene, follicle-size dependent
mRNA expression is increased by forskolin and overexpression of the catalytic subunit of protein kinase A,. Furthermore, treatment with a soluble epidermal growth factor induces mRNA expression in granulosa cells. The enzyme expression is increased at 6 to 24 hours after human chorionic gonadotropin in granulosa cells
transiently expressed in the infarcted heart, when stimulated with tissue necrosis factor, the expression level of mRNA rapidly increases
-
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
diagnostics
ADAMTS1 is potential prognostic biomarker promotes malignant invasion of glioma
medicine
medicine
in men not on pravastatin, those homozygous for the 227Pro allele of ADAMTS1 have a nearly 2-fold increased risk of coronary heart disease events compared with noncarriers. In this high-risk group, treatment with pravastatin is highly efficacious, reducing the odds of fatal coronary disease or nonfatal myocardial infarction by approximately 75%, as compared with 25% in noncarriers or heterozygotes
medicine
ADAMTS1, ADAMTS5 and follicle stimulating hormone receptors are expressed 2.56, 2.10, and 2.66fold less in Sertoli cells of NOA patients, than those of obstructive azoospermia. After recombinant follicle stimulating hormone is added onto Sertoli cell cultures of patients with nonobstructive azoospermia, their expression does not increase significantly and does not reach to levels of control group. Expression of ADAMTS1 and ADAMTS5 are insignificantly 1.03 and 1.1fold higher in obstructive azoospermia group, respectively. In the nonobstructive azoospermia group, their expressions are 1.70 and 1.96fold lower, respectively, when compared with the fertile control
medicine
syngeneic B16F1 melanoma model in wild type and ADAMTS1 knockout mice. Genetic deletion of ADAMTS1 in the host microenvironment results in a drastic decrease of tumor growth and metastasis. Reduced tumors in ADAMTS1 KO mice display a paradoxical increase in vascular density and vascular-related genes with an impaired vasculature, along with a minor infiltration of macrophages. ADAMTS1is involved in vascular sprouting, and melanoma xenografts show a relevant induction of its expression in stroma compartments
medicine
strong, diffuse staining is observed throughout myocardial tissue for ADAMTS1 both in individuals who died of myocardial infarction and a group who died of trauma. Among patients who died of myocardial infarction, no expression for ADAMTS1 is observed around fibrotic areas but slight staining in coagulative and necrotic zones
medicine
expression of a majority of the investigated ADAMTS members ADAMTS-1, 4,5,6,8,9,10,13,17 on mRNA level is decreased in aneurysm compared to control aorta. ADAMTS-1 is one of the members that is reduced most. ADAMTS-1 is present predominantly in areas of smooth muscle cells and macrophages in aneurysmal aorta and higher expressed in abdominal aortic aneurysm compared to control aortas
medicine
mean serum ADAMTS-1 level is increased in adolescents and younger-aged females with polycystic ovary syndrome (PCOS) compared to ovulatory controls. An elevated ADAMTS-1 level is positive predictive of the diagnosis of PCOS with the best cut-off value of 2.5 ng/ml. A positive predictive role of ADAMTS-1 on the development of cardiovascular disease risk and insulin resistance is found among all patients. Serum ADAMTS-1 and aggrecan levels are significantly and positively correlated with each other
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Boeckmann, B.; Bairoch, A.; Apweiler, R.; Blatter, M.C.; Estreicher, A.; Gasteiger, E.; Martin M.J.; Michoud, K.; O'Donovan, C.; Phan, I.; Pilbout, S.; Schneider, M.
The SWISS-PROT protein knowledgebase and its supplement TrEMBL
Nucleic Acids Res.
31
365-370
2003
Mus musculus (P97857), Rattus norvegicus (Q9WUQ1)
brenda
Sandy, J.D.; Westling, J.; Kenagy, R.D.; Iruela-Arispe, M.L.; Verscharen, C.; Rodriguez-Mazaneque, J.C.; Zimmermann, D.R.; Lemire, J.M.; Fischer, J.W.; Wight, T.N.; Clowes, A.W.
Versican V1 proteolysis in human aorta in vivo occurs at the Glu441-Ala442 bond, a site that is cleaved by recombinant ADAMTS-1 and ADAMTS-4
J. Biol. Chem.
276
13372-13378
2001
Homo sapiens
brenda
Iruela-Arispe, M.L.; Carpizo, D.; Luque, A.
ADAMTS1: a matrix metalloprotease with angioinhibitory properties
Ann. N. Y. Acad. Sci.
995
183-190
2003
Homo sapiens
brenda
Rodriguez-Manzaneque, J.C.; Westling, J.; Thai, S.N.; Luque, A.; Knauper, V.; Murphy, G.; Sandy, J.D.; Iruela-Arispe, M.L.
ADAMTS1 cleaves aggrecan at multiple sites and is differentially inhibited by metalloproteinase inhibitors
Biochem. Biophys. Res. Commun.
293
501-508
2002
Homo sapiens
brenda
Vankemmelbeke, M.N.; Jones, G.C.; Fowles, C.; Ilic, M.Z.; Handley, C.J.; Day, A.J.; Knight, C.G.; Mort, J.S.; Buttle, D.J.
Selective inhibition of ADAMTS-1, -4 and -5 by catechin gallate esters
Eur. J. Biochem.
270
2394-2403
2003
Homo sapiens (Q9UHI8)
brenda
Kuno, K.; Okada, Y.; Kawashima, H.; Nakamura, H.; Miyasaka, M.; Ohno, H.; Matsushima, K.
ADAMTS-1 cleaves a cartilage proteoglycan, aggrecan
FEBS Lett.
478
241-245
2000
Mus musculus
brenda
Tang, B.L.
ADAMTS: a novel family of extracellular matrix proteases
Int. J. Biochem. Cell Biol.
33
33-44
2001
Homo sapiens (Q9UHI8), Mus musculus
brenda
Kuno, K.; Terashima, Y.; Matsushima, K.
ADAMTS-1 is an active metalloproteinase associated with the extracellular matrix
J. Biol. Chem.
274
18821-18826
1999
Mus musculus
brenda
Vazquez, F.; Hastings, G.; Ortega, M.A.; Lane, T.F.; Oikemus, S.; Lombardo, M.; Iruela-Arispe, M.L.
METH-1, a human ortholog of ADAMTS-1, and METH-2 are members of a new family of proteins with angio-inhibitory activity
J. Biol. Chem.
274
23349-23357
1999
Homo sapiens (Q9UHI8), Homo sapiens
brenda
Rodriguez-Manzaneque, J.C.; Milchanowski, A.B.; Dufour, E.K.; Leduc, R.; Iruela-Arispe, M.L.
Characterization of METH-1/ADAMTS1 processing reveals two distinct active forms
J. Biol. Chem.
275
33471-33479
2000
Homo sapiens
brenda
Luque, A.; Carpizo, D.R.; Iruela-Arispe, M.L.
ADAMTS1/METH1 inhibits endothelial cell proliferation by direct binding and sequestration of VEGF165
J. Biol. Chem.
278
23656-23665
2003
Homo sapiens
brenda
Kaushal, G.P.; Shah, S.V.
The new kids on the block: ADAMTSs, potentially multifunctional metalloproteinases of the ADAM family
J. Clin. Invest.
105
1335-1337
2000
Mus musculus
brenda
Guenther, W.; Skaftnesmo, K.O.; Arnold, H.; Bjerkvig, R.; Terzis, A.J.
Distribution patterns of the anti-angiogenic protein ADAMTS-1 during rat development
Acta Histochem.
107
121-131
2005
Rattus norvegicus
brenda
Kuno, K.; Bannai, K.; Hakozaki, M.; Matsushima, K.; Hirose, K.
The carboxyl-terminal half region of ADAMTS-1 suppresses both tumorigenicity and experimental tumor metastatic potential
Biochem. Biophys. Res. Commun.
319
1327-1333
2004
Mus musculus
brenda
Mittaz, L.; Russell, D.L.; Wilson, T.; Brasted, M.; Tkalcevic, J.; Salamonsen, L.A.; Hertzog, P.J.; Pritchard, M.A.
Adamts-1 is essential for the development and function of the urogenital system
Biol. Reprod.
70
1096-1105
2004
Mus musculus
brenda
Cross, A.K.; Haddock, G.; Stock, C.J.; Allan, S.; Surr, J.; Bunning, R.A.; Buttle, D.J.; Woodroofe, M.N.
ADAMTS-1 and -4 are up-regulated following transient middle cerebral artery occlusion in the rat and their expression is modulated by TNF in cultured astrocytes
Brain Res.
1088
19-30
2006
Rattus norvegicus
brenda
Suga, A.; Hikasa, H.; Taira, M.
Xenopus ADAMTS1 negatively modulates FGF signaling independent of its metalloprotease activity
Dev. Biol.
295
26-39
2006
Xenopus laevis
brenda
Krampert, M.; Kuenzle, S.; Thai, S.N.; Lee, N.; Iruela-Arispe, M.L.; Werner, S.
ADAMTS1 proteinase is up-regulated in wounded skin and regulates migration of fibroblasts and endothelial cells
J. Biol. Chem.
280
23844-23852
2005
Mus musculus
brenda
Lee, N.V.; Rodriguez-Manzaneque, J.C.; Thai, S.N.; Twal, W.O.; Luque, A.; Lyons, K.M.; Argraves, W.S.; Iruela-Arispe, M.L.
Fibulin-1 acts as a cofactor for the matrix metalloprotease ADAMTS-1
J. Biol. Chem.
280
34796-34804
2005
Homo sapiens (Q9UHI8)
brenda
Torres-Collado, A.X.; Kisiel, W.; Iruela-Arispe, M.L.; Rodriguez-Manzaneque, J.C.
ADAMTS1 interacts with, cleaves, and modifies the extracellular location of the matrix inhibitor tissue factor pathway inhibitor-2
J. Biol. Chem.
281
17827-17837
2006
Homo sapiens
brenda
Shozu, M.; Minami, N.; Yokoyama, H.; Inoue, M.; Kurihara, H.; Matsushima, K.; Kuno, K.
ADAMTS-1 is involved in normal follicular development, ovulatory process and organization of the medullary vascular network in the ovary
J. Mol. Endocrinol.
35
343-355
2005
Mus musculus
brenda
Lind, T.; Birch, M.A.; McKie, N.
Purification of an insect derived recombinant human ADAMTS-1 reveals novel gelatin (type I collagen) degrading activities
Mol. Cell. Biochem.
281
95-102
2006
Homo sapiens (Q9UHI8)
brenda
Liu, Y.J.; Xu, Y.; Yu, Q.
Full-length ADAMTS-1 and the ADAMTS-1 fragments display pro- and antimetastatic activity, respectively
Oncogene
25
2452-2467
2006
Mus musculus (P97857)
brenda
Cross, N.A.; Chandrasekharan, S.; Jokonya, N.; Fowles, A.; Hamdy, F.C.; Buttle, D.J.; Eaton, C.L.
The expression and regulation of ADAMTS-1, -4, -5, -9, and -15, and TIMP-3 by TGFbeta1 in prostate cells: relevance to the accumulation of versican
Prostate
63
269-275
2005
Homo sapiens
brenda
Basile, D.P.; Fredrich, K.; Chelladurai, B.; Leonard, E.C.; Parrish, A.R.
Renal ischemia reperfusion inhibits VEGF expression and induces ADAMTS-1, a novel VEGF inhibitor
Am. J. Physiol. Renal Physiol.
294
F928-F936
2008
Rattus norvegicus
brenda
Rehn, A.P.; Birch, M.A.; Karlstroem, E.; Wendel, M.; Lind, T.
ADAMTS-1 increases the three-dimensional growth of osteoblasts through type I collagen processing
Bone
41
231-238
2007
Homo sapiens
brenda
Lind, G.E.; Kleivi, K.; Meling, G.I.; Teixeira, M.R.; Thiis-Evensen, E.; Rognum, T.O.; Lothe, R.A.
ADAMTS1, CRABP1, and NR3C1 identified as epigenetically deregulated genes in colorectal tumorigenesis
Cell. Oncol.
28
259-272
2006
Homo sapiens
brenda
Brown, H.M.; Dunning, K.R.; Robker, R.L.; Pritchard, M.; Russell, D.L.
Requirement for ADAMTS-1 in extracellular matrix remodeling during ovarian folliculogenesis and lymphangiogenesis
Dev. Biol.
300
699-709
2006
Mus musculus
brenda
Stankunas, K.; Hang, C.T.; Tsun, Z.Y.; Chen, H.; Lee, N.V.; Wu, J.I.; Shang, C.; Bayle, J.H.; Shou, W.; Iruela-Arispe, M.L.; Chang, C.P.
Endocardial Brg1 represses ADAMTS1 to maintain the microenvironment for myocardial morphogenesis
Dev. Cell
14
298-311
2008
Mus musculus
brenda
Lee, N.V.; Sato, M.; Annis, D.S.; Loo, J.A.; Wu, L.; Mosher, D.F.; Iruela-Arispe, M.L.
ADAMTS1 mediates the release of antiangiogenic polypeptides from TSP1 and 2
EMBO J.
25
5270-5283
2006
Homo sapiens
brenda
Kalinski, T.; Krueger, S.; Sel, S.; Werner, K.; Roepke, M.; Roessner, A.
ADAMTS1 is regulated by interleukin-1beta, not by hypoxia, in chondrosarcoma
Hum. Pathol.
38
86-94
2007
Homo sapiens
brenda
Gerhardt, S.; Hassall, G.; Hawtin, P.; McCall, E.; Flavell, L.; Minshull, C.; Hargreaves, D.; Ting, A.; Pauptit, R.A.; Parker, A.E.; Abbott, W.M.
Crystal structures of human ADAMTS-1 reveal a conserved catalytic domain and a disintegrin-like domain with a fold homologous to cysteine-rich domains
J. Mol. Biol.
373
891-902
2007
Homo sapiens (Q9UHI8)
brenda
Canals, F.; Colome, N.; Ferrer, C.; Plaza-Calonge, M.d.e.l.C.; Rodriguez-Manzaneque, J.C.
Identification of substrates of the extracellular protease ADAMTS1 by DIGE proteomic analysis
Proteomics
6 Suppl 1
S28-S35
2006
Homo sapiens
brenda
Sabatine, M.S.; Ploughman, L.; Simonsen, K.L.; Iakoubova, O.A.; Kirchgessner, T.G.; Ranade, K.; Tsuchihashi, Z.; Zerba, K.E.; Long, D.U.; Tong, C.H.; Packard, C.J.; Pfeffer, M.A.; Devlin, J.J.; Shepherd, J.; Campos, H.; Sacks, F.M.; Braunwald, E.
Association between ADAMTS1 matrix metalloproteinase gene variation, coronary heart disease, and benefit of statin therapy
Arterioscler. Thromb. Vasc. Biol.
28
562-567
2008
Homo sapiens (Q9UHI8), Homo sapiens
brenda
Rodriguez-Manzaneque, J.C.; Carpizo, D.; Plaza-Calonge, M.d.e.l..C.; Torres-Collado, A.X.; Thai, S.N.; Simons, M.; Horowitz, A.; Iruela-Arispe, M.L.
Cleavage of syndecan-4 by ADAMTS1 provokes defects in adhesion
Int. J. Biochem. Cell Biol.
41
800-810
2009
Homo sapiens (Q9UHI8)
brenda
Hatipoglu, O.F.; Hirohata, S.; Cilek, M.Z.; Ogawa, H.; Miyoshi, T.; Obika, M.; Demircan, K.; Shinohata, R.; Kusachi, S.; Ninomiya, Y.
ADAMTS1 is a unique hypoxic early response gene expressed by endothelial cells
J. Biol. Chem.
284
16325-16333
2009
Homo sapiens
brenda
Diamantis, I.; Luethi, M.; Hoesli, M.; Reichen, J.
Cloning of the rat ADAMTS-1 gene and its down regulation in endothelial cells in cirrhotic rats
Liver
20
165-172
2000
Rattus norvegicus (Q9WUQ1)
brenda
Pockert, A.J.; Richardson, S.M.; Le Maitre, C.L.; Lyon, M.; Deakin, J.A.; Buttle, D.J.; Freemont, A.J.; Hoyland, J.A.
Modified expression of the ADAMTS enzymes and tissue inhibitor of metalloproteinases 3 during human intervertebral disc degeneration
Arthritis Rheum.
60
482-491
2009
Homo sapiens (Q9UHI8)
brenda
Hamel, M.G.; Ajmo, J.M.; Leonardo, C.C.; Zuo, F.; Sandy, J.D.; Gottschall, P.E.
Multimodal signaling by the ADAMTSs (a disintegrin and metalloproteinase with thrombospondin motifs) promotes neurite extension
Exp. Neurol.
210
428-440
2008
Homo sapiens
brenda
Takizawa, M.; Yatabe, T.; Okada, A.; Chijiiwa, M.; Mochizuki, S.; Ghosh, P.; Okada, Y.
Calcium pentosan polysulfate directly inhibits enzymatic activity of ADAMTS4 (aggrecanase-1) in osteoarthritic chondrocytes
FEBS Lett.
582
2945-2949
2008
Homo sapiens
brenda
Demircan, K.; Gunduz, E.; Gunduz, M.; Beder, L.B.; Hirohata, S.; Nagatsuka, H.; Cengiz, B.; Cilek, M.Z.; Yamanaka, N.; Shimizu, K.; Ninomiya, Y.
Increased mRNA expression of ADAMTS metalloproteinases in metastatic foci of head and neck cancer
Head Neck
10
793-801
2009
Homo sapiens
brenda
Hatipoglu, O.F.; Hirohata, S.; Yaykasli, K.O.; Cilek, M.Z.; Demircan, K.; Shinohata, R.; Yonezawa, T.; Oohashi, T.; Kusachi, S.; Ninomiya, Y.
The 3-untranslated region of ADAMTS1 regulates its mRNA stability
Acta Med. Okayama
63
79-85
2009
Homo sapiens
brenda
Brown, H.M.; Dunning, K.R.; Robker, R.L.; Boerboom, D.; Pritchard, M.A.; Lane, M.; Russell, D.L.
ADAMTS1 cleavage of versican mediates essential structural remodeling of the ovarian follicle and cumulus-oocyte matrix during ovulation in mice
Biol. Reprod.
83
549-557
2010
Mus musculus
brenda
Curry, T.E.
ADAMTS1 and versican: partners in ovulation and fertilization
Biol. Reprod.
83
505-506
2010
Mus musculus
brenda
Gustavsson, H.; Tesan, T.; Jennbacken, K.; Kuno, K.; Damber, J.E.; Welen, K.
ADAMTS1 alters blood vessel morphology and TSP1 levels in LNCaP and LNCaP-19 prostate tumors
BMC Cancer
10
288
2010
Homo sapiens
brenda
Keightley, M.C.; Sales, K.J.; Jabbour, H.N.
PGF2alpha-F-prostanoid receptor signalling via ADAMTS1 modulates epithelial cell invasion and endothelial cell function in endometrial cancer
BMC Cancer
10
488
2010
Homo sapiens
brenda
Casal, C.; Torres-Collado, A.X.; Plaza-Calonge, M.d.e.l..C.; Martino-Echarri, E.; Ramon Y Cajal, S.; Rojo, F.; Griffioen, A.W.; Rodriguez-Manzaneque, J.C.
ADAMTS1 contributes to the acquisition of an endothelial-like phenotype in plastic tumor cells
Cancer Res.
70
4676-4686
2010
Homo sapiens
brenda
Lee, Y.J.; Koch, M.; Karl, D.; Torres-Collado, A.X.; Fernando, N.T.; Rothrock, C.; Kuruppu, D.; Ryeom, S.; Iruela-Arispe, M.L.; Yoon, S.S.
Variable inhibition of thrombospondin 1 against liver and lung metastases through differential activation of metalloproteinase ADAMTS1
Cancer Res.
70
948-956
2010
Mus musculus
brenda
Guo, C.; Wang, Y.; Liang, H.; Zhang, J.
ADAMTS-1 contributes to the antifibrotic effect of captopril by accelerating the degradation of type I collagen in chronic viral myocarditis
Eur. J. Pharmacol.
629
104-110
2010
Mus musculus
brenda
Lu, X.; Wang, Q.; Hu, G.; Van Poznak, C.; Fleisher, M.; Reiss, M.; Massague, J.; Kang, Y.
ADAMTS1 and MMP1 proteolytically engage EGF-like ligands in an osteolytic signaling cascade for bone metastasis
Genes Dev.
23
1882-1894
2009
Homo sapiens
brenda
Esselens, C.; Malapeira, J.; Colome, N.; Casal, C.; Rodriguez-Manzaneque, J.C.; Canals, F.; Arribas, J.
The cleavage of semaphorin 3C induced by ADAMTS1 promotes cell migration
J. Biol. Chem.
285
2463-2473
2010
Homo sapiens (Q9UHI8)
brenda
Hohberg, M.; Knoechel, J.; Hoffmann, C.J.; Chlench, S.; Wunderlich, W.; Alter, A.; Maroski, J.; Vorderwuelbecke, B.J.; Silva-Azevedo, L.D.; Knudsen, R.; Lehmann, R.; Fiedorowicz, K.; Bongrazio, M.; Nitsche, B.; Hoepfner, M.; Styp-Rekowska, B.; Pries, A.R.; Zakrzewicz, A.
Expression of ADAMTS1 in endothelial cells is induced by shear stress and suppressed in sprouting capillaries
J. Cell. Physiol.
226
350-361
2011
Homo sapiens
brenda
Yung, Y.; Maman, E.; Konopnicki, S.; Cohen, B.; Brengauz, M.; Lojkin, I.; Dal Canto, M.; Fadini, R.; Dor, J.; Hourvitz, A.
ADAMTS-1: a new human ovulatory gene and a cumulus marker for fertilization capacity
Mol. Cell. Endocrinol.
328
104-108
2010
Homo sapiens (Q9UHI8), Homo sapiens (Q8NE26), Homo sapiens
brenda
Hu, L.; Jonsson, K.B.; Andersen, H.; Edenro, A.; Bohlooly-Y, M.; Melhus, H.; Lind, T.
Over-expression of Adamts1 in mice alters bone mineral density
J. Bone Miner. Metab.
30
304-311
2011
Mus musculus
brenda
Fu, Y.; Nagy, J.A.; Brown, L.F.; Shih, S.C.; Johnson, P.Y.; Chan, C.K.; Dvorak, H.F.; Wight, T.N.
Proteolytic cleavage of versican and involvement of ADAMTS-1 in VEGF-A/VPF-induced pathological angiogenesis
J. Histochem. Cytochem.
59
463-473
2011
Mus musculus
brenda
Sayasith, K.; Lussier, J.; Sirois, J.
Molecular characterization and transcriptional regulation of a disintegrin and metalloproteinase with thrombospondin motif 1 (ADAMTS1) in bovine preovulatory follicles
Endocrinology
154
2857-2869
2013
Bos taurus (A7MB07), Bos taurus
brenda
Inagaki, J.; Takahashi, K.; Ogawa, H.; Asano, K.; Faruk Hatipoglu, O.; Cilek, M.Z.; Obika, M.; Ohtsuki, T.; Hofmann, M.; Kusachi, S.; Ninomiya, Y.; Hirohata, S.
ADAMTS1 inhibits lymphangiogenesis by attenuating phosphorylation of the lymphatic endothelial cell-specific VEGF receptor
Exp. Cell Res.
323
263-275
2014
Homo sapiens (Q9UHI8), Homo sapiens
brenda
Hong-Brown, L.Q.; Brown, C.R.; Navaratnarajah, M.; Lang, C.H.
Adamts1 mediates ethanol-induced alterations in collagen and elastin via a FoxO1-sestrin3-AMPK signaling cascade in myocytes
J. Cell. Biochem.
116
91-101
2015
Mus musculus (P97857)
brenda
Toba, H.; de Castro Bras, L.E.; Baicu, C.F.; Zile, M.R.; Lindsey, M.L.; Bradshaw, A.D.
Increased ADAMTS1 mediates SPARC-dependent collagen deposition in the aging myocardium
Am. J. Physiol. Endocrinol. Metab.
310
E1027-E1035
2016
Mus musculus (P97857)
brenda
Tola, E.N.; Koroglu, N.D.; Yalcin, S.E.; Oral, H.B.
The role of serum ADAMTS-1 and aggrecan on polycystic ovary syndrome in adolescents and younger-aged females
Arch. Gynecol. Obstet.
297
487-493
2018
Homo sapiens (Q9UHI8)
brenda
Noriega-Guerra, H.; Cruz, M.C.; Ribeiro, P.R.L.; Strnadel, J.; Wang, H.; Klemke, R.L.; Jaeger, R.G.; Freitas, V.M.
ADAMTS-1 disrupts HGF/c-MET signaling and HGF-stimulated cellular processes in fibrosarcoma
Exp. Cell Res.
363
271-282
2018
Homo sapiens
brenda
Pehlivan, S.; Gurses, M.S.; Ural, M.N.; Akyol, S.; Eren, F.; Turkmen Inanir, N.; Gulec, M.A.; Eren, B.; Fedakar, R.; Demircan, K.; Akyol, O.
The Role of ADAMTS1 and versican in human myocardial infarction a postmortem study
Lab. Med.
47
205-212
2016
Homo sapiens (Q9UHI8), Homo sapiens
brenda
Du, H.; Shih, C.; Wosczyna, M.; Mueller, A.; Cho, J.; Aggarwal, A.; Rando, T.; Feldman, B.
Macrophage-released ADAMTS1 promotes muscle stem cell activation
Nat. Commun.
8
669
2017
Mus musculus (P97857)
brenda
Fernandez-Rodriguez, R.; Rodriguez-Baena, F.J.; Martino-Echarri, E.; Peris-Torres, C.; Del Carmen Plaza-Calonge, M.; Rodriguez-Manzaneque, J.C.
Stroma-derived but not tumor ADAMTS1 is a main driver of tumor growth and metastasis
Oncotarget
7
34507-34519
2016
Mus musculus (P97857)
brenda
Silva, S.V.; Lima, M.A.; Cella, N.; Jaeger, R.G.; Freitas, V.M.
ADAMTS-1 is found in the nuclei of normal and tumoral breast cells
PLoS ONE
11
e0165061
2016
Homo sapiens (Q9UHI8)
brenda
Vorkapic, E.; Folkesson, M.; Magnell, K.; Bohlooly-Y, M.; Laenne, T.; Wagsaeter, D.
ADAMTS-1 in abdominal aortic aneurysm
PLoS ONE
12
e0178729
2017
Homo sapiens (Q9UHI8), Mus musculus (P97857)
brenda
Rodriguez-Baena, F.J.; Redondo-Garcia, S.; Peris-Torres, C.; Martino-Echarri, E.; Fernandez-Rodriguez, R.; Plaza-Calonge, M.D.C.; Anderson, P.; Rodriguez-Manzaneque, J.C.
ADAMTS1 protease is required for a balanced immune cell repertoire and tumour inflammatory response
Sci. Rep.
8
13103
2018
Mus musculus (P97857)
brenda
Aydos, O.S.; Yukselten, Y.; Ozkavukcu, S.; Sunguroglu, A.; Aydos, K.
ADAMTS1 and ADAMTS5 metalloproteases produced by Sertoli cells a potential diagnostic marker in azoospermia
Syst. Biol. Reprod. Med.
65
29-38
2019
Homo sapiens (Q9UHI8), Homo sapiens
brenda
Hu, X.; Jiang, C.; Hu, N.; Hong, S.
ADAMTS1 induces epithelial-mesenchymal transition pathway in non-small cell lung cancer by regulating TGF-beta
Aging
15
2097-2114
2023
Mus musculus (P97857)
brenda
Peris-Torres, C.; Plaza-Calonge, M.D.C.; Lopez-Dominguez, R.; Dominguez-Garcia, S.; Barrientos-Duran, A.; Carmona-Saez, P.; Rodriguez-Manzaneque, J.C.
Extracellular protease ADAMTS1 is required at early stages of human uveal melanoma development by inducing stemness and endothelial-like features on tumor cells
Cancers (Basel)
12
801
2020
Homo sapiens (Q9UHI8)
brenda
Tan, I.A.; Frewin, K.; Ricciardelli, C.; Russell, D.L.
ADAMTS1 promotes adhesion to extracellular matrix proteins and predicts prognosis in early stage breast cancer patients
Cell. Physiol. Biochem.
52
1553-1568
2019
Mus musculus (P97857)
brenda
Wang, S.; Zhang, J.; Wang, K.; Zhao, Y.; Liu, D.
ADAMTS1 as potential prognostic biomarker promotes malignant invasion of glioma
Int. J. Clin. Oncol.
28
52-68
2023
Homo sapiens (Q9UHI8)
brenda
Minns, A.F.; Qi, Y.; Yamamoto, K.; Lee, K.; Ahnstroem, J.; Santamaria, S.
The C-terminal domains of ADAMTS1 contain exosites involved in its proteoglycanase activity
J. Biol. Chem.
299
103048
2023
Homo sapiens (Q9UHI8)
brenda
Mead, T.J.; Bhutada, S.; Foulcer, S.J.; Peruzzi, N.; Nelson, C.M.; Seifert, D.E.; Larkin, J.; Tran-Lundmark, K.; Filmus, J.; Apte, S.S.
Combined genetic-pharmacologic inactivation of tightly linked ADAMTS proteases in temporally specific windows uncovers distinct roles for versican proteolysis and glypican-6 in cardiac development
Matrix Biol.
131
1-16
2024
Mus musculus (P97857)
brenda
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