Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.24.B11 extracted from

  • Kuno, K.; Okada, Y.; Kawashima, H.; Nakamura, H.; Miyasaka, M.; Ohno, H.; Matsushima, K.
    ADAMTS-1 cleaves a cartilage proteoglycan, aggrecan (2000), FEBS Lett., 478, 241-245.
    View publication on PubMed
Search for more literature for 3.4.24.B11

Cloned(Commentary)

Cloned (Comment) Organism
transient expression in HEK293 cells of FLAG- and His-tagged wild-type and deletion mutants, the majority of the recombinant protein is expressed in an active form without the proprotein domain Mus musculus

Protein Variants

Protein Variants Comment Organism
E386Q oligonucleotide primer mutagenesis, mutation within the zinc binding motif, inactive Mus musculus
additional information construction of soluble C-terminal deletion mutants Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
 Mus musculus
additional information no inhibition by aprotinin and leupeptin Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular matrix Mus musculus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information metalloproteinase Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
aggrecan + H2O Mus musculus substrate is a cartilage proteoglycan ?
-
 ?
proteins + H2O Mus musculus
-
 peptides
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type and mutants Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
cartilage
-
 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
aggrecan + H2O C-terminal spacer region of the enzyme is required for activity, substrate is a cartilage proteoglycan, cleavage site is the peptide bond Glu1871-Leu1872 within the substrates chondroitin sulfate attachment domain Mus musculus ?
-
 ?
aggrecan + H2O substrate is a cartilage proteoglycan Mus musculus ?
-
 ?
proteins + H2O
-
 Mus musculus peptides
-
 ?

Synonyms

Synonyms Comment Organism
a disintegrin and metalloproteinase with thrombospondin motifs 1
-
 Mus musculus
ADAMTS-1
-
 Mus musculus
M12.222 Merops-ID Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
 assay at Mus musculus