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Literature summary for 3.4.24.B11 extracted from
- METH-1, a human ortholog of ADAMTS-1, and METH-2 are members of a new family of proteins with angio-inhibitory activity (1999), J. Biol. Chem., 274, 23349-23357.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, in itro translation, expression as His-tagged protein in Escherichia coli strain BL21 | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | secretion | Homo sapiens | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 67000 | - |
x * 67000, recombinant processed form 2, SDS-PAGE, x * 85000, recombinant processed form 1, SDS-PAGE, x * 110000, recombinant unprocessed enzyme, SDS-PAGE | Homo sapiens |
| 85000 | - |
x * 67000, recombinant processed form 2, SDS-PAGE, x * 85000, recombinant processed form 1, SDS-PAGE, x * 110000, recombinant unprocessed enzyme, SDS-PAGE | Homo sapiens |
| 110000 | - |
x * 67000, recombinant processed form 2, SDS-PAGE, x * 85000, recombinant processed form 1, SDS-PAGE, x * 110000, recombinant unprocessed enzyme, SDS-PAGE | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Homo sapiens | enzyme inhibits endothelial cell proliferation, but not fibroblast or smooth muscle growth | ? | - |
? |  |
| proteins + H2O | Homo sapiens | - |
peptides | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q9UHI8 | single copy gene | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | cleavage at the consensus subtilisin site RKKR resulting in 2 different processed forms of MW 85 kDa and 67 kDa | Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21 | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adrenal gland | - |
Homo sapiens | - |
| heart | - |
Homo sapiens | - |
| kidney | embryonic | Homo sapiens | - |
| additional information | no activity in brain, liver, pancreas | Homo sapiens | - |
| placenta | - |
Homo sapiens | - |
| skeletal muscle | - |
Homo sapiens | - |
| stomach | - |
Homo sapiens | - |
| thyroid gland | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | enzyme inhibits fibroblast growth factor 2-induced vascularization in the cornea pocket assay and inhibits vascular endothelial growth factor-induced angiogenesis in the chorioallantoic membrane assay | Homo sapiens | ? | - |
? | |
| additional information | enzyme inhibits endothelial cell proliferation, but not fibroblast or smooth muscle growth | Homo sapiens | ? | - |
? | |
| proteins + H2O | - |
Homo sapiens | peptides | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 67000, recombinant processed form 2, SDS-PAGE, x * 85000, recombinant processed form 1, SDS-PAGE, x * 110000, recombinant unprocessed enzyme, SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| M12.222 | Merops-ID | Homo sapiens |
| METH-1 | - |
Homo sapiens |
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