Information on EC 3.4.21.4 - trypsin

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
3.4.21.4
-
RECOMMENDED NAME
GeneOntology No.
trypsin
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
preferential cleavage: Arg-/-, Lys-/-
show the reaction diagram
catalytic mechanism involving both His57 and Asp102, overview
-
preferential cleavage: Arg-/-, Lys-/-
show the reaction diagram
mechanism
-
preferential cleavage: Arg-/-, Lys-/-
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
carboxylic acid amide hydrolysis
-
-
hydrolysis of peptide bond
-
-
endopeptidase
-
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
acrosin
-
serine protease with trypsin-like activity specific of the spermatozoa
alpha-trypsin
-
-
-
-
alpha-trypsin
-
-
alpha-trypsin
-
-
anionic salmon trypsin
salmon
-
-
anionic trypsin
-
-
Anionic trypsinogen
-
-
-
-
AST
salmon
-
-
Atlantic cod trypsin I
-
-
beta-trypsin
-
-
-
-
BPT
-
-
BPT
-
-
Brain trypsinogen
-
-
-
-
cationic trypsin
-
-
Cationic trypsinogen
-
-
-
-
cocoonase
-
-
-
-
CST
Q8AV11
-
EC 3.4.4.4
-
-
formerly
-
fibrinolytic enzyme
-
-
group III trypsin
-
-
Mesotrypsinogen
-
-
-
-
midgut-specific serine protease 1
O76519
-
parenzyme
-
-
-
-
parenzymol
-
-
-
-
PPT
-
-
pseudotrypsin
-
-
-
-
SET
-
-
-
-
sperm receptor hydrolase
-
-
-
-
ST-1
-
isozyme
ST-2
-
isozyme
ST-3
-
isozyme
TIIA
-
isozyme
TIII
-
isozyme
tripcellim
-
-
-
-
TRP
-
-
TRY-3
-
-
TryI
-
isozyme
TryII
-
isozyme
TryIII
-
isozyme
trypsin 3A1
P29786
-
trypsin 4
-
-
trypsin A
-
-
trypsin B
-
-
trypsin I
-
-
trypsin I
-
-
trypsin IV
-
-
trypsin type III
-
-
trypsin Y
-
-
trypsin-1
-
isozyme
trypsin-2
-
isozyme
trypsin-3
-
-
trypsin-4
-
brain isozyme
trypsin-like enzyme
-
-
trypsin-like serine proteinase
-
-
tryptar
-
-
-
-
tryptase
-
-
-
-
trypure
-
-
-
-
TrypZean
-
bovine-sequence trypsin solution produced from corn, commercial recombinant enzyme preparation
type I trypsin
-
-
type IX pancreatic trypsin
-
-
midgut-specific serine protease 2
O76520
-
additional information
B3IYE4
trypsin is a member of the large family of serine proteases
CAS REGISTRY NUMBER
COMMENTARY
9002-07-7
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
alpha-trypsin and beta-trypsin
-
-
Manually annotated by BRENDA team
calf
-
-
Manually annotated by BRENDA team
isoform beta-trypsin
-
-
Manually annotated by BRENDA team
Cambarus affinis
-
-
-
Manually annotated by BRENDA team
a anionic and a cationic trypsin
-
-
Manually annotated by BRENDA team
Sugarcane rootstalk borer weevil
UniProt
Manually annotated by BRENDA team
sugar cane stalk borer
-
-
Manually annotated by BRENDA team
sharpsnout sea bream
-
-
Manually annotated by BRENDA team
isoform EFE-b
-
-
Manually annotated by BRENDA team
walleye pollock
-
-
Manually annotated by BRENDA team
recombinant enzyme
-
-
Manually annotated by BRENDA team
HzTrypsin-C
SwissProt
Manually annotated by BRENDA team
HzTrypsin-S
SwissProt
Manually annotated by BRENDA team
isoform trypsin-2
-
-
Manually annotated by BRENDA team
isozymes trypsin-1, -2, -C and -3
-
-
Manually annotated by BRENDA team
pancreatic isoforms cationic trypsin, anionic trypsin, and mesotrypsin
-
-
Manually annotated by BRENDA team
putative trypsin
UniProt
Manually annotated by BRENDA team
fragment; formerly Penaeus vannamei
UniProt
Manually annotated by BRENDA team
isoforms A,B,C
-
-
Manually annotated by BRENDA team
tobacco hornworm moth
UniProt
Manually annotated by BRENDA team
chum salmon
-
-
Manually annotated by BRENDA team
isoform TR-P
-
-
Manually annotated by BRENDA team
diamondback moth
-
-
Manually annotated by BRENDA team
red palm weevil
-
-
Manually annotated by BRENDA team
trypsin-like protease; synonym Saccharopolyspora erythraea
UniProt
Manually annotated by BRENDA team
salmon
-
-
-
Manually annotated by BRENDA team
Atlantic bonito
-
-
Manually annotated by BRENDA team
trypsin isozymes A, B and C
-
-
Manually annotated by BRENDA team
isoform TR-S
-
-
Manually annotated by BRENDA team
insecticide-resistant and -susceptible strains, serine proteinases with trypsin-like activity
-
-
Manually annotated by BRENDA team
precursor
UniProt
Manually annotated by BRENDA team
commercial preparation
-
-
Manually annotated by BRENDA team
commercial preparation, modified by supplier using reductive methylation and N-tosyl-Phe chloromethylketone treatment
-
-
Manually annotated by BRENDA team
type IX, commercial preparation
-
-
Manually annotated by BRENDA team
yellowfin tuna
-
-
Manually annotated by BRENDA team
Triops sp.
-
-
-
Manually annotated by BRENDA team
Mexican bean weevil
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
malfunction
-
trypsin from pancreatic acinar cells destroys islets. Thus pancreatic slet transplantation which has emerged as an effective therapy and potential cure for type 1 diabetes mellitus, requires inhibition of trypsin activity by ulinastatin or pefabloc, optimization of islet preservation using the two-layer method, TLM, overview
physiological function
-
active trypsin participates in the digestion of food by degradation of dietary proteins and by activation of other digestive enzymes in the small intestine
physiological function
-
trypsin is a digestive enzyme
physiological function
B3IYE4
trypsin is a major digestive enzyme
physiological function
D0G7G2
trypsin is a major digestive enzyme
physiological function
C4TP29
trypsin is a major digestive enzyme
malfunction
-
infection of Lutzomyia longipalpis with Leishmania mexicana reduces trypsin-like activity in the sand fly midgut. Inhibition of trypsin expression by RNAi enhances the survival of Leishmania mexicana in Lutzomyia longipalpis, overview
additional information
-
enhanced serine proteinase activity may be playing a role in mitigating physiological costs associated with the maintenance of insecticide resistance mechanisms in some maize weevil strains
additional information
-
trypsin 1 is the major blood meal induced trypsin activity in the sand fly midgut
additional information
-
analysis of trypsin immunogenicity in male CBA mice compared to other serine proteases, overview
additional information
B3IYE4
completely conserved major structural features common to trypsin such as the catalytic triad, formed by His57, Asp102, and Ser195, the obligatory Asp189 and twelve Cys residues, but with deletion of Tyr151 and substitution of Pro152 for Gly in the autolysis loop
additional information
D0G7G2
the enzyme shows completely conserved structural features for catalytic function of trypsins, but possesses the four Met residues, Met135, Met145, Met175, and Met242, in their molecules and the deletion of Tyr151 and substitution of Pro152 for Gly in their autolysis loops
additional information
C4TP29
the enzyme shows completely conserved structural features for catalytic function of trypsins, but possesses the four Met residues, Met135, Met145, Met175, and Met242, in their molecules and the deletion of Tyr151 and substitution of Pro152 for Gly in their autolysis loops
additional information
-
detailed geometry of the active site and the protonation state of catalytic residues, quantum chemical interpretation, overview
additional information
-
a fibronectin type III-like peptide from aqueous extract of human placenta, used as a licensed drug for wound healing, tightly complexes the enzyme and regulates its activity, near-irreversible binding, modeling, overview
additional information
-
thrombin and trypsin directly activate vagal C-fibres in C57BL6 mouse lung via protease-activated receptor-1, i.e. PAR1, not via PAR3, PAR2, and PAR4, overview
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(benzyloxycarbonyl-L-alanyl-L-arginine)2-rhodamine-110 + 2 H2O
2 benzyloxycarbonyl-L-alanyl-L-arginine + rhodamine-110
show the reaction diagram
-
specific trypsin substrate
-
-
?
4-methylumbelliferyl-4-guanidinobenzoate + H2O
4-methylumbelliferol + 4-guanidinobenzoate
show the reaction diagram
-
-
-
-
?
Abz-ERFK-EDDnp + H2O
Abz-ER + FK-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-FRFK-EDDnp + H2O
Abz-FR + FK-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-GRFK-EDDnp + H2O
Abz-GR + FK-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-HRFK-EDDnp + H2O
Abz-HR + FK-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-KRFK-EDDnp + H2O
Abz-KR + FK-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-LRFK-EDDnp + H2O
Abz-LR + FK-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-NRFK-EDDnp + H2O
Abz-NR + FK-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-QRFK-EDDnp + H2O
Abz-QR + FK-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-RDFK-EDDnp + H2O
Abz-R + DFK-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-REFK-EDDnp + H2O
Abz-R + EFK-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-RGFK-EDDnp + H2O
Abz-R + GFK-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-RHFK-EDDnp + H2O
Abz-R + HFK-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-RNFK-EDDnp + H2O
Abz-R + NFK-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-RPFK-EDDnp + H2O
Abz-R + PFK-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-RQFK-EDDnp + H2O
Abz-R + QFK-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-RRFK-EDDnp + H2O
Abz-R + RFK-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-RSFK-EDDnp + H2O
Abz-R + SFK-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-RTFK-EDDnp + H2O
Abz-R + TFK-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-RVFK-EDDnp + H2O
Abz-R + VFK-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-RYFK-EDDnp + H2O
Abz-R + YFK-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-VRFK-EDDnp + H2O
Abz-VR + FK-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-WRFK-EDDnp + H2O
Abz-WR + FK-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-YRFK-EDDnp + H2O
Abz-YR + FK-EDDnp
show the reaction diagram
-
-
-
-
?
acetyl-Ala-Val-(L-alpha-aminobutyryl)-Pro-Arg(NO2)-4-nitroanilide + H2O
?
show the reaction diagram
-
-
-
?
acetyl-Ala-Val-(L-alpha-aminobutyryl)-Pro-Lys-4-nitroanilide + H2O
?
show the reaction diagram
-
-
-
?
acetyl-Ala-Val-(L-alpha-aminobutyryl)-Pro-N-omega-hydroxy-L-arginine-4-nitroanilide + H2O
?
show the reaction diagram
-
-
-
?
acetyl-Ala-Val-(L-alpha-aminobutyryl)-Pro-Phe(p-CN)-4-nitroanilide + H2O
?
show the reaction diagram
-
-
-
?
acetyl-Ala-Val-(L-alpha-aminobutyryl)-Pro-Phe(p-NH2)-4-nitroanilide + H2O
?
show the reaction diagram
-
-
-
?
acetyl-L-Phe-L-Ala-L-Thr-(4-guanidino)-L-Phe-NH-(3-carbamoyl-4-nitrophenol) + H2O
acetyl-L-Phe-L-Ala-L-Thr-(4-guanidino)-L-Phe + 5-amino-2-nitrobenzamide
show the reaction diagram
-
-
-
-
?
AGPYAHSS + H2O
?
show the reaction diagram
-
trypsin N143H/E151H hydrolyzes the peptide exclusively in presence of Ni2+ or Zn2+ with high levels of catalytic efficiency
-
-
?
alpha-amylase + H2O
?
show the reaction diagram
P00775
-
-
-
?
alpha-casein + H2O
?
show the reaction diagram
-
-
-
-
?
alpha-N-p-tosyl-L-arginine methyl ester + H2O
alpha-N-p-tosyl-L-arginine + methanol
show the reaction diagram
-
-
-
-
?
ANVIPFKVHFRAAFC + H2O
ANVIPFK + VHFR + AAFC
show the reaction diagram
-
-
-
-
?
apomyoglobin + H2O
?
show the reaction diagram
-
-
-
-
?
Arg-4-nitroanilide + H2O
Arg + 4-nitroaniline
show the reaction diagram
-, O18434, Q9NB82
-
-
?
benzoyl-Arg 4-nitroanilide + H2O
benzoyl-Arg + 4-nitroanilide
show the reaction diagram
-
-
-
?
benzoyl-Arg ethyl ester + H2O
benzoyl-Arg + ethanol
show the reaction diagram
-
-
-
?
benzoyl-Arg ethyl ester + leucinamide
benzoyl-Arg leucinamide + ethanol
show the reaction diagram
-
-
-
?
benzoyl-Arg-4-nitroanilide + H2O
benzoyl-Arg + 4-nitroaniline
show the reaction diagram
-, O18434, Q9NB82
-
-
?
benzoyl-Arg-p-nitroanilide + H2O
benzoyl-Arg + p-nitroaniline
show the reaction diagram
-
-
-
-
?
benzoyl-DL-Arg-4-nitroanilide + H2O
benzoyl-DL-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
benzoyl-DL-Arg-7-amido-4-methylcoumarin + H2O
benzoyl-DL-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
benzoyl-DL-Arg-p-nitroanilide + H2O
benzoyl-DL-Arg + p-nitroaniline
show the reaction diagram
-
-
-
-
?
benzoyl-DL-arginine-p-nitroanilide + H2O
benzoyl-DL-arginine + p-nitroaniline
show the reaction diagram
-
-
-
-
?
benzoyl-L-Arg amide + H2O
?
show the reaction diagram
-
beta-trypsin is about 40% more active than alpha trypsin
-
-
?
benzoyl-L-Arg-4-nitroanilide + H2O
benzoyl-L-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
benzoyl-L-arginine-p-nitroanilide + H2O
benzoyl-L-arginine + p-nitroaniline
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Arg-4-nitroanilide + H2O
benzyloxycarbonyl-Arg + 4-nitrolaniline
show the reaction diagram
-, O18434, Q9NB82
-
-
?
benzyloxycarbonyl-Arg-Arg-4-nitroanilide + H2O
?
show the reaction diagram
-, O18434, Q9NB82
-
-
?
benzyloxycarbonyl-Arg-thiobenzyl ester + H2O
benzyloxycarbonyl-Arg + phenylmethanethiol
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Gly-Pro-Arg-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Gly-Pro-Arg-p-nitroanilide + H2O
benzyloxycarbonyl-Gly-Pro-Arg + p-nitroaniline
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-L-glutaminyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide + H2O
benzyloxycarbonyl-L-glutaminyl-L-arginyl-L-arginine + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-L-glutamoyl-L-lysyl-L-lysine-4-methylcoumaryl-7-amide + H2O
benzyloxycarbonyl-L-glutamoyl-L-lysyl-L-lysine + 7-amino-4-methylcoumarin
show the reaction diagram
-
low activity
-
-
?
benzyloxycarbonyl-L-leucyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide + H2O
benzyloxycarbonyl-L-leucyl-L-arginyl-L-arginine + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-L-leucyl-L-lysyl-L-arginine-4-methylcoumaryl-7-amide + H2O
benzyloxycarbonyl-L-leucyl-L-lysyl-L-arginine + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-L-phenylalaninyl-L-seryl-L-arginine-4-methylcoumaryl-7-amide + H2O
benzyloxycarbonyl-L-phenylalaninyl-L-seryl-L-arginine + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-L-valyl-L-leucyl-L-lysine-4-methylcoumaryl-7-amide + H2O
benzyloxycarbonyl-L-valyl-L-leucyl-L-lysine + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-L-valyl-L-prolyl-L-arginine-4-methylcoumaryl-7-amide + H2O
benzyloxycarbonyl-L-valyl-L-prolyl-L-arginine + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
Benzyloxycarbonyl-Lys methyl ester + H2O
Benzyloxycarbonyl-Lys + methanol
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Lys p-nitrophenyl ester + H2O
benzyloxycarbonyl-Lys + p-nitrophenol
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Lys-thiobenzyl ester + H2O
benzyloxycarbonyl-Lys + phenylmethanethiol
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Lys-thiobenzyl ester + H2O
benzyloxycarbonyl-Lys + phenylmethanethiol
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Phe-Arg-4-nitroanilide + H2O
?
show the reaction diagram
-, O18434, Q9NB82
-
-
?
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
-
-
-
?
benzyloxycarbonylglycyl-L-prolyl-L-arginine-4-methylcoumaryl-7-amide + H2O
benzyloxycarbonylglycyl-L-prolyl-L-arginine + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
benzyloxycarbopnyl-L-Lys benzoyl ester + H2O
benzyloxycarbopnyl-L-Lys + benzoate
show the reaction diagram
-
-
-
-
?
beta-crystallin + H2O
?
show the reaction diagram
-
-
-
-
?
beta-lactoglobulin + H2O
?
show the reaction diagram
-
-
-
-
?
bovine heart cytochrome c + H2O
?
show the reaction diagram
-
-
-
-
?
Bovine serum albumin + H2O
?
show the reaction diagram
-
-
-
-
?
Bovine serum albumin + H2O
?
show the reaction diagram
-
-
-
-
?
Bovine serum albumin + H2O
?
show the reaction diagram
P00775
-
-
-
?
bovine serum albumin + H2O
LVVSTQTALA + ?
show the reaction diagram
-
-
-
-
?
bovine skin DQ-collagen type I + H2O
?
show the reaction diagram
-
-
-
-
?
calmodulin + H2O
?
show the reaction diagram
-
-
-
-
?
carbobenzoxy-L-Arg-7-amido-4-methylcoumarin + H2O
carbobenzoxy-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
carbonic anhydrase + H2O
?
show the reaction diagram
P00775
-
-
-
?
casein + H2O
?
show the reaction diagram
-
-
-
-
?
casein + H2O
?
show the reaction diagram
-
-
-
-
?
casein + H2O
?
show the reaction diagram
-
-
-
-
?
casein + H2O
?
show the reaction diagram
-
-
-
-
?
casein + H2O
?
show the reaction diagram
-
-
-
-
?
casein + H2O
?
show the reaction diagram
-
beta-casein, mutant enzymes K188E and K188Y cleave nearly 30 new peptide bonds compared to wild-type enzyme
-
-
?
Cbz-L-Arg-7-amido-4-methylcoumarin + H2O
Cbz-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
Cbz-L-Arg-7-amino-4-methylcoumarin + H2O
Cbz-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
Collagen + H2O
?
show the reaction diagram
-
trypsin has little activity against collagen
-
-
?
Cry1Ab protoxin + H2O
Cry1Ab toxin
show the reaction diagram
-
-
-
-
?
Cytochrome c + H2O
?
show the reaction diagram
-
-
-
-
?
Cytochrome c + H2O
?
show the reaction diagram
P00775
-
-
-
?
D-Phe-Pip-Arg 4-nitroanilide + H2O
D-Phe-Pip-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
D-Phe-piperidinyl-Arg-4-nitroanilide + H2O
?
show the reaction diagram
-
-
-
?
D-Val-Leu-Arg-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
-
-
-
?
D-Val-Leu-Lys-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
-
-
-
?
D-Val-Leu-Lys-thiobenzyl ester + H2O
?
show the reaction diagram
-
-
-
-
?
DrmSP8-36 + H2O
?
show the reaction diagram
-
trypsin is the major endopeptidase regulating N-terminal truncated sex peptide DrmSP8-36 in hemolymph, the trypsin cleavage site R7-K8 is essential for release of the C-terminal fragment SP8-36 from sperm tail, the protein is cleaved more rapidly and completely in mated female hemolymph
-
-
?
enhanced green fluorescent protein-T1 + H2O
?
show the reaction diagram
-
enhanced green fluorescent protein-T1 is specifically cleaved into two major fragments by trypsin at the grafted cleavage site, approximately 20 and 8 kDa
-
-
?
enhanced green fluorescent protein-T1nb + H2O
?
show the reaction diagram
-
-
-
-
?
enhanced green fluorescent protein-T2 + H2O
?
show the reaction diagram
-
enhanced green fluorescent protein-T2 is specifically cleaved into two major fragments by trypsin at the grafted cleavage site, approximately 18 and 10 kDa
-
-
?
epithelial sodium channel + H2O
?
show the reaction diagram
-
trypsin activates epithelial sodium channels in native renal tubules
-
-
?
factor Xa + H2O
?
show the reaction diagram
-
-
-
-
?
Fibronectin + H2O
?
show the reaction diagram
-
-
-
-
?
FITC-casein + H2O
?
show the reaction diagram
-
fluorescence-labeled casein
-
-
?
gamma-crystallin + H2O
?
show the reaction diagram
-
-
-
-
?
GDDSTPSILPAPRGYPGQV + H2O
GDDSTPSILPAPR + GYPGQV
show the reaction diagram
-
-
-
-
?
Gelatin + H2O
?
show the reaction diagram
-
-
-
-
?
Glu-Gly-Arg-4-nitroanilide + H2O
?
show the reaction diagram
-
-
-
?
glucose oxidase + H2O
?
show the reaction diagram
P00775
-
-
-
?
Gly-Pro-Arg-p-nitroanilide + H2O
Gly-Pro-Arg + p-nitroaniline
show the reaction diagram
-
-
-
-
?
glycogen phosphorylase b + H2O
?
show the reaction diagram
P00775
-
-
-
?
GPNSKGRSLIGRLDTPYGGC + H2O
GPNSKGR + SLIGRLDTPYGGC
show the reaction diagram
-
-
-
-
?
Hemoglobin + H2O
?
show the reaction diagram
-
-
-
-
?
Hemoglobin + H2O
?
show the reaction diagram
P00775
-
-
-
?
high pathogenic hemagglutinin + H2O
?
show the reaction diagram
-
-
-
-
?
human cartilage collagen type II + H2O
?
show the reaction diagram
-
-, use of enzyme and its regulators as markers of prognosis and disease activity in rheumatoid arthritis
-
-
?
human placenta DQ-collagen type IV + H2O
?
show the reaction diagram
-
-
-
-
?
insulin + H2O
?
show the reaction diagram
-
oxidized A chain, and B chain
-
-
?
insulin + H2O
?
show the reaction diagram
-
oxidized and S-aminomethylated B-chain of trypsin. The enzyme hydrolyzes peptide bonds in which the carbonyl group is contributed by Arg, Lys and S-2-aminoethyl-cysteine
-
-
?
insulin + H2O
?
show the reaction diagram
-
S-sulfonated B-chain of bovine insulin, only the linkages Arg22-Gly23 and Lys29-Ala30 are hydrolyzed
-
-
?
inter-alpha-trypsin inhibitor heavy chain H4 + H2O
?
show the reaction diagram
-
inter-alpha-trypsin inhibitor heavy chain H4 is also a marker of acute ischemic stroke
-
-
?
intestinal alkaline sphingomyelinase bound to mucosa + H2O
free intestinal alkaline sphingomyelinase
show the reaction diagram
-
cleavage at R440, first tryptic site upstream of signal anchor. Physiological role of enzyme in sphingomyelin digestion, cleavage at R440, first tryptic site upstream of signal anchor
-
-
?
L-Phe-L-Pro-L-Arg-7-amido-4-methylcoumarin + H2O
L-Phe-L-Pro-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
L-Phe-L-Val-L-Ile-N-(3-carbamoyl-4-nitrophenyl)-L-Arg-amide + H2O
L-Phe-L-Val-L-Ile-L-Arg + 5-amino-2-nitrobenzamide
show the reaction diagram
-
-
-
-
?
L-Phe-L-Val-L-Ile-N-(3-carbamoyl-4-nitrophenyl)-L-Lys-amide + H2O
L-Phe-L-Val-L-Ile-L-Lys + 5-amino-2-nitrobenzamide
show the reaction diagram
-
-
-
-
?
L-Phe-L-Val-L-Pro-N-(3-carbamoyl-4-nitrophenyl)-L-Arg-amide + H2O
L-Phe-L-Val-L-Pro-L-Arg + 5-amino-2-nitrobenzamide
show the reaction diagram
-
-
-
-
?
L-Phe-L-Val-L-Pro-N-(3-carbamoyl-4-nitrophenyl)-L-Lys-amide + H2O
L-Phe-L-Val-L-Pro-L-Lys + 5-amino-2-nitrobenzamide
show the reaction diagram
-
-
-
-
?
L-Tat (47-58) + H2O
?
show the reaction diagram
-
trypsin significantly inactivates L-Tat (47-58)
-
-
?
low pathogenic hemagglutinin + H2O
?
show the reaction diagram
-
-
-
-
?
lubricin + H2O
?
show the reaction diagram
-
-
-
-
?
Lysozyme + H2O
?
show the reaction diagram
P00775
-
-
-
?
MeOSuc-Ala-Ala-Ala-Arg 4-nitroanilide + H2O
MeOSuc-Ala-Ala-Ala-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
MeOSuc-Ala-Ala-Arg 4-nitroanilide + H2O
MeOSuc-Ala-Ala-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
Myoglobin + H2O
?
show the reaction diagram
P00775
-
-
-
?
N-acetyl-GTNRSSKGRSLIGKVDGTSHVTGKGVT-amide + H2O
SLIGKVDGTSHVTGKGVT-amide + N-acetyl-GTNRSSKGR
show the reaction diagram
-
-
-
-
?
N-alpha-benzoyl-DL-Arg-4-nitroanilide + H2O
N-alpha-benzoyl-DL-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
N-alpha-benzoyl-DL-Arg-4-nitroanilide + H2O
N-alpha-benzoyl-DL-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
N-alpha-benzoyl-DL-Arg-4-nitroanilide + H2O
N-alpha-benzoyl-DL-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
N-alpha-benzoyl-DL-Arg-4-nitroanilide + H2O
N-alpha-benzoyl-DL-Arg + 4-nitroaniline
show the reaction diagram
-
trypsin-specific substrate
-
-
?
N-alpha-benzoyl-DL-arginine-4-nitroanilide + H2O
N-alpha-benzoyl-DL-arginine + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
N-alpha-benzoyl-DL-arginine-4-nitroanilide + H2O
N-alpha-benzoyl-DL-arginine + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
N-alpha-benzoyl-DL-arginine-p-nitroanilide + H2O
N-alpha-benzoyl-DL-arginine + p-nitroaniline
show the reaction diagram
-
-
-
-
?
N-alpha-benzoyl-DL-arginine-p-nitroanilide + H2O
N-alpha-benzoyl-DL-arginine + p-nitroaniline
show the reaction diagram
-
-
-
-
?
N-alpha-benzoyl-L-Arg ethyl ester + H2O
N-alpha-benzoyl-L-Arg + ethanol
show the reaction diagram
-
-
-
-
?
N-alpha-benzoyl-L-Arg ethyl ester + H2O
N-alpha-benzoyl-L-Arg + ethanol
show the reaction diagram
-
-
-
-
?
N-alpha-benzoyl-L-Arg methyl ester + H2O
N-alpha-benzoyl-L-Arg + methanol
show the reaction diagram
-
-
-
-
?
N-alpha-benzoyl-L-Arg-2-naphthylamide + H2O
N-alpha-benzoyl-L-Arg + 2-naphthylamine
show the reaction diagram
-
-
-
-
?
N-alpha-benzoyl-L-Arg-4-nitroanilide + H2O
N-alpha-benzoyl-L-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
N-alpha-benzoyl-L-Arg-4-nitroanilide + H2O
N-alpha-benzoyl-L-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
N-alpha-benzoyl-L-Arg-4-nitroanilide + H2O
N-alpha-benzoyl-L-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
N-alpha-benzoyl-L-Arg-4-nitroanilide + H2O
N-alpha-benzoyl-L-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
N-alpha-benzoyl-L-Arg-4-nitroanilide + H2O
N-alpha-benzoyl-L-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
N-alpha-benzoyl-L-Arg-4-nitroanilide + H2O
N-alpha-benzoyl-L-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
N-alpha-benzoyl-L-Arg-4-nitroanilide + H2O
N-alpha-benzoyl-L-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
N-alpha-benzoyl-L-Arg-4-nitroanilide + H2O
N-alpha-benzoyl-L-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
N-alpha-benzoyl-L-Arg-4-nitroanilide + H2O
N-alpha-benzoyl-L-Arg + 4-nitroaniline
show the reaction diagram
Q54137, -
-
-
-
?
N-alpha-benzoyl-L-Arg-ethyl ester + H2O
N-alpha-benzoyl-L-Arg + ethanol
show the reaction diagram
-
-
-
-
?
N-alpha-benzoyl-L-Arg-ethyl ester + H2O
N-alpha-benzoyl-L-Arg + ethanol
show the reaction diagram
-
-
-
-
?
N-alpha-benzoyl-L-arginine 4-nitroanilide + H2O
N-alpha-benzoyl-L-arginine + 4-nitroanilide
show the reaction diagram
-
-
-
?
N-alpha-benzoyl-L-arginine ethyl ester + H2O
?
show the reaction diagram
-
-
-
-
?
N-alpha-benzoyl-L-arginine ethyl ester + H2O
?
show the reaction diagram
-
-
-
-
?
N-alpha-benzoyl-L-arginine ethyl ester + H2O
?
show the reaction diagram
-
-
-
-
?
N-alpha-benzoyl-L-arginine ethyl ester + H2O
N-alpha-benzoyl-L-arginine + ethanol
show the reaction diagram
-
-
-
-
?
N-alpha-benzoyl-L-arginine ethyl ester + H2O
N-alpha-benzoyl-L-arginine + ethanol
show the reaction diagram
-
-
-
-
?
N-alpha-benzoyl-L-arginine-p-nitroanilide + H2O
N-alpha-benzoyl-L-arginine + p-nitroaniline
show the reaction diagram
-
-
-
-
?
N-alpha-benzoyl-L-Lys-p-nitroanilide + H2O
N-alpha-benzoyl-L-Lys + p-nitroaniline
show the reaction diagram
-
-
-
-
?
N-alpha-benzoyl-L-Lys-p-nitroanilide + H2O
N-alpha-benzoyl-L-Lys + p-nitroaniline
show the reaction diagram
Q54137, -
Streptomyces erythraeus trypsin has more than 1 order of magnitude greater amidase activity than mammalian trypsin
-
-
?
N-alpha-benzoyl-L-Phe-L-Val-L-Arg-4-nitroanilide + H2O
N-alpha-benzoyl-L-Phe-L-Val-L-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
N-alpha-benzoyl-L-Pro-L-Phe-L-Arg-4-nitroanilide + H2O
N-alpha-benzoyl-L-Pro-L-Phe-L-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
N-alpha-p-tosyl-L-Arg methyl ester + H2O
N-alpha-p-tosyl-L-Arg + methanol
show the reaction diagram
-
-
-
-
?
N-alpha-p-tosyl-L-arginine methyl ester + H2O
N-alpha-p-tosyl-L-arginine + ?
show the reaction diagram
-
-
-
-
?
N-alpha-p-tosyl-L-arginine methyl ester + H2O
N-alpha-p-tosyl-L-arginine + methanol
show the reaction diagram
-
-
-
-
?
N-alpha-p-tosyl-L-arginine methyl ester + H2O
N-alpha-p-tosyl-L-arginine + ethanol
show the reaction diagram
-
-
-
-
?
N-benzoyl-DL-Arg-4-nitroanilide + H2O
N-benzoyl-DL-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
N-benzoyl-DL-Arg-4-nitroanilide + H2O
N-benzoyl-L-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
N-benzoyl-DL-arginine 4-nitroanilide + H2O
N-benzoyl-DL-arginine + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
N-benzoyl-DL-arginine 4-nitroanilide + H2O
N-benzoyl-DL-arginine + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
N-benzoyl-DL-arginine 4-nitroanilide + H2O
N-benzoyl-DL-arginine + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
N-benzoyl-DL-arginine 4-nitroanilide + H2O
N-benzoyl-DL-arginine + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
N-benzoyl-DL-arginine 4-nitroanilide + H2O
N-benzoyl-DL-arginine + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
N-benzoyl-DL-arginine 4-nitroanilide + H2O
N-benzoyl-DL-arginine + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
N-benzoyl-DL-arginine 4-nitroanilide + H2O
N-benzoyl-DL-arginine + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
N-benzoyl-DL-arginine 4-nitroanilide + H2O
N-benzoyl-DL-arginine + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
N-benzoyl-DL-arginine 4-nitroanilide + H2O
N-benzoyl-DL-arginine + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
N-benzoyl-DL-arginine 4-nitroanilide + H2O
N-benzoyl-DL-arginine + 4-nitroaniline
show the reaction diagram
-
a synthetic trypsin substrate
-
-
?
N-benzoyl-DL-arginine 4-nitroanilide + H2O
N-benzoyl-DL-arginine + 4-nitroaniline
show the reaction diagram
-
a synthetic trypsin substrate
-
-
?
N-benzoyl-DL-arginine 4-nitroanilide + H2O
N-benzoyl-DL-arginine + 4-nitroaniline
show the reaction diagram
-
a synthetic trypsin substrate
-
-
?
N-benzoyl-DL-arginine 4-nitroanilide + H2O
N-benzoyl-DL-arginine + 4-nitroaniline
show the reaction diagram
-
a synthetic trypsin substrate
-
-
?
N-benzoyl-DL-arginine 4-nitroanilide + H2O
N-benzoyl-DL-arginine + 4-nitroaniline
show the reaction diagram
-
a synthetic trypsin substrate
-
-
?
N-benzoyl-DL-arginine-4-nitroanilide + H2O
N-benzoyl-DL-arginine + 4-nitroaniline
show the reaction diagram
P00775
-
-
-
?
N-benzoyl-DL-arginyl-4-nitroanilide + H2O
?
show the reaction diagram
-
-
-
?
N-Benzoyl-L-Arg ethyl ester + H2O
?
show the reaction diagram
-
-
-
-
?
N-Benzoyl-L-Arg ethyl ester + H2O
?
show the reaction diagram
-
-
-
-
?
N-Benzoyl-L-Arg ethyl ester + H2O
?
show the reaction diagram
-
-
-
-
?
N-Benzoyl-L-Arg ethyl ester + H2O
?
show the reaction diagram
-
-
-
-
?
N-benzoyl-L-Arg-4-nitroanilide + H2O
N-benzoyl-L-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
N-benzoyl-L-arginine 4-nitroanilide + H2O
N-benzoyl-L-arginine + 4-nitroaniline
show the reaction diagram
-
the trypsin-like proteinases from the resistant strains exhibited higher affinity for the substrate than the enzymes from the susceptible strains
-
-
?
N-benzoyl-L-arginine ethyl ester + H2O
N-benzoyl-L-arginine + ethanol
show the reaction diagram
-
-
-
-
?
N-benzoyl-L-arginine-p-nitroanilide + H2O
N-benzoyl-L-arginine + p-nitroaniline
show the reaction diagram
-
-
-
-
?
N-benzoyl-L-isoleucyl-L-glutamyl-glycyl-L-arginine methyl ester + H2O
N-benzoyl-L-isoleucyl-L-glutamyl-glycyl-L-arginine + methanol
show the reaction diagram
-
-
-
-
?
N-benzoyl-L-isoleucyl-L-glutamyl-glycyl-L-arginine-4-nitroanilide + H2O
N-benzoyl-L-isoleucyl-L-glutamyl-glycyl-L-arginine + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
N-benzyloxycarbonyl-Gly-L-Pro-L-Arg-4-nitroanilide + H2O
N-benzyloxycarbonyl-Gly-L-Pro-L-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
N-benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide + H2O
N-benzyloxycarbonyl-Gly-Pro-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
N-benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
N-benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
N-benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
N-benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
N-benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
N-benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
N-carbobenzyloxy-Gly-Pro-Arg-p-nitroanilide + H2O
N-carbobenzyloxy-Gly-Pro-Arg + p-nitroaniline
show the reaction diagram
-
-
-
-
?
N-carbobenzyloxy-Gly-Pro-Arg-p-nitroanilide + H2O
N-carbobenzyloxy-Gly-Pro-Arg + p-nitroaniline
show the reaction diagram
-
-
-
-
?
Nafamostat + H2O
?
show the reaction diagram
-
extremely poor substrate
-
?
Nalpha-(tert-butoxycarbonyl)-L-alanyl-L-alanine-4-nitroanilide + H2O
Nalpha-(tert-butoxycarbonyl)-L-alanyl-L-alanine + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
Nalpha-4-tosyl-L-Arg methyl ester + H2O
?
show the reaction diagram
-
-
-
-
?
Nalpha-4-tosyl-L-arginine methyl ester + H2O
Nalpha-4-tosyl-L-arginine + methanol
show the reaction diagram
-
-
-
-
?
Nalpha-4-tosyl-L-arginine methyl ester + H2O
Nalpha-4-tosyl-L-arginine + methanol
show the reaction diagram
-
-
-
-
?
Nalpha-4-tosyl-L-arginine methyl ester + H2O
Nalpha-4-tosyl-L-arginine + methanol
show the reaction diagram
-
-
-
-
?
Nalpha-benzoyl-DL-Arg 2-naphthylamide + H2O
?
show the reaction diagram
-
-
-
-
?
Nalpha-benzoyl-DL-Arg-p-nitroanilide + H2O
?
show the reaction diagram
-
-
-
-
?
Nalpha-benzoyl-DL-Arg-p-nitroanilide + H2O
?
show the reaction diagram
-
-
-
-
?
Nalpha-benzoyl-DL-Arg-p-nitroanilide + H2O
?
show the reaction diagram
-
-
-
-
?
Nalpha-benzoyl-DL-Arg-p-nitroanilide + H2O
?
show the reaction diagram
-
-
-
-
?
Nalpha-benzoyl-DL-Arg-p-nitroanilide + H2O
?
show the reaction diagram
-
Nalpha-benzoyl-L-Arg-p-nitroanilide
-
-
?
Nalpha-benzoyl-DL-Arg-p-nitroanilide + H2O
?
show the reaction diagram
-
Nalpha-benzoyl-L-Arg-p-nitroanilide
-
-
?
Nalpha-benzoyl-DL-arginine 4-nitroanilide + H2O
Nalpha-benzoyl-DL-arginine + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
Nalpha-benzoyl-DL-arginine 4-nitroanilide + H2O
Nalpha-benzoyl-DL-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
Nalpha-benzoyl-DL-arginine-4-nitroanilide + H2O
Nalpha-benzoyl-DL-arginine + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
Nalpha-benzoyl-L-Arg-4-nitroanilide + H2O
Nalpha-benzoyl-L-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
Nalpha-benzoyl-L-arginine ethyl ester + H2O
Nalpha-benzoyl-L-arginine + ethanol
show the reaction diagram
-
-
-
-
?
Nalpha-benzoyl-L-arginine ethyl ester + H2O
Nalpha-benzoyl-L-arginine + ethanol
show the reaction diagram
-
-
-
-
?
Nalpha-Boc-D-Ala-Gly-(4-carbamidamido) phenyl ester + L-Ala-4-nitroanilide
Nalpha-Boc-D-Ala-L-Ala-4-nitroanilide + 1-(4-hydroxyphenyl)guanidine
show the reaction diagram
-
-
-
-
?
Nalpha-Boc-D-Ala-Gly-(4-carbamido)phenyl ester + D-Ala-4-nitroanilide
Nalpha-Boc-D-Ala-D-Ala-4-nitroanilide + 4-hydroxybenzimidamide
show the reaction diagram
-
-
-
-
?
Nalpha-Boc-D-Ala-Gly-(4-carbamido)phenyl ester + L-Ala-4-nitroanilide
Nalpha-Boc-D-Ala-L-Ala-4-nitroanilide + 4-hydroxybenzimidamide
show the reaction diagram
-
-
-
-
?
Nalpha-Boc-D-Leu-Gly-(4-carbamidamido) phenyl ester + L-Ala-4-nitroanilide
Nalpha-Boc-D-Leu-L-Ala-4-nitroanilide + 1-(4-hydroxyphenyl)guanidine
show the reaction diagram
-
-
-
-
?
Nalpha-Boc-D-Leu-Gly-(4-carbamido)phenyl ester + L-Ala-4-nitroanilide
Nalpha-Boc-D-Leu-L-Ala-4-nitroanilide + 4-hydroxybenzimidamide
show the reaction diagram
-
-
-
-
?
Nalpha-Boc-D-Phe-Gly-(4-carbamidamido) phenyl ester + L-Ala-4-nitroanilide
Nalpha-Boc-D-Phe-L-Ala-4-nitroanilide + 1-(4-hydroxyphenyl)guanidine
show the reaction diagram
-
-
-
-
?
Nalpha-Boc-D-Phe-Gly-(4-carbamido)phenyl ester + L-Ala-4-nitroanilide
Nalpha-Boc-D-Phe-L-Ala-4-nitroanilide + 4-hydroxybenzimidamide
show the reaction diagram
-
-
-
-
?
Nalpha-Boc-Gly-Gly-(4-carbamidamido) phenyl ester + L-Ala-4-nitroanilide
Nalpha-Boc-Gly-L-Ala-4-nitroanilide + 1-(4-hydroxyphenyl)guanidine
show the reaction diagram
-
-
-
-
?
Nalpha-Boc-Gly-Gly-(4-carbamido)phenyl ester + L-Ala-4-nitroanilide
Nalpha-Boc-Gly-L-Ala-4-nitroanilide + 4-hydroxybenzimidamide
show the reaction diagram
-
-
-
-
?
Nalpha-Boc-L-Ala-Gly-(4-carbamidamido) phenyl ester + L-Ala-4-nitroanilide
Nalpha-Boc-L-Ala-L-Ala-4-nitroanilide + 1-(4-hydroxyphenyl)guanidine
show the reaction diagram
-
-
-
-
?
Nalpha-Boc-L-Ala-Gly-(4-carbamido)phenyl ester + D-Ala-4-nitroanilide
Nalpha-Boc-L-Ala-D-Ala-4-nitroanilide + 4-hydroxybenzimidamide
show the reaction diagram
-
-
-
-
?
Nalpha-Boc-L-Ala-Gly-(4-carbamido)phenyl ester + L-Ala-4-nitroanilide
Nalpha-Boc-L-Ala-L-Ala-4-nitroanilide + 4-hydroxybenzimidamide
show the reaction diagram
-
-
-
-
?
Nalpha-Boc-L-Leu-Gly-(4-carbamidamido) phenyl ester + L-Ala-4-nitroanilide
Nalpha-Boc-L-Leu-L-Ala-4-nitroanilide + 1-(4-hydroxyphenyl)guanidine
show the reaction diagram
-
-
-
-
?
Nalpha-Boc-L-Leu-Gly-(4-carbamido)phenyl ester + L-Ala-4-nitroanilide
Nalpha-Boc-L-Leu-L-Ala-4-nitroanilide + 4-hydroxybenzimidamide
show the reaction diagram
-
-
-
-
?
Nalpha-Boc-L-Phe-Gly-(4-carbamidamido) phenyl ester + L-Ala-4-nitroanilide
Nalpha-Boc-L-Phe-L-Ala-4-nitroanilide + 1-(4-hydroxyphenyl)guanidine
show the reaction diagram
-
-
-
-
?
Nalpha-Boc-L-Phe-Gly-(4-carbamido)phenyl ester + L-Ala-4-nitroanilide
Nalpha-Boc-L-Phe-L-Ala-4-nitroanilide + 4-hydroxybenzimidamide
show the reaction diagram
-
-
-
-
?
Nalpha-p-tosyl-L-Arg methyl ester + H2O
?
show the reaction diagram
-
-
-
?
Nalpha-p-tosyl-L-Arg methyl ester + H2O
?
show the reaction diagram
-
-
-
-
?
Nalpha-p-tosyl-L-arginine methyl ester + H2O
?
show the reaction diagram
-
-
-
-
?
NATLDPRSFLLRNPNDKYE + H2O
NATLDPR + SFLLRNPNDKYE
show the reaction diagram
-
-
-
-
?
Nbeta-Boc-beta-Ala-Gly-(4-carbamidamido) phenyl ester + beta-Ala-4-nitroanilide
Nbeta-Boc-beta-Ala-beta-Ala-4-nitroanilide + 1-(4-hydroxyphenyl)guanidine
show the reaction diagram
-
-
-
-
?
Nbeta-Boc-beta-Ala-Gly-(4-carbamidamido) phenyl ester + L-Ala-4-nitroanilide
Nbeta-Boc-beta-Ala-L-Ala-4-nitroanilide + 1-(4-hydroxyphenyl)guanidine
show the reaction diagram
-
-
-
-
?
Nbeta-Boc-beta-Ala-Gly-(4-carbamido)phenyl ester + L-Ala-4-nitroanilide
Nbeta-Boc-beta-Ala-L-Ala-4-nitroanilide + 4-hydroxybenzimidamide
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AAGRGAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AAGR + GAGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AAGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-ADGRGAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-ADGR + GAGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-ADGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AEGRGAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AEGR + GAGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AEGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AFGRGAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AFGR + GAGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AFGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AGARGAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGAR + GAGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AGARGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AGDRGAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGDR + GAGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AGDRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AGERGAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGER + GAGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AGERGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AGFRGAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGFR + GAGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AGFRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AGGKGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AGGRAAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GAGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AGGRDAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GDGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AGGREAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GEGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AGGRFAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GFGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AGGRGAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GGGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AGGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AGGRGDGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AGGRGEGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AGGRGFGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AGGRGGGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AGGRGHGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AGGRGIGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AGGRGKGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AGGRGLGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AGGRGMGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AGGRGNGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AGGRGQGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AGGRGSGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AGGRGTGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AGGRGVGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AGGRGYGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AGGRHAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GHGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AGGRIAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GIGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AGGRKAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GKGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AGGRLAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GLGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AGGRMAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GMGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AGGRNAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GNGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AGGRQAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GQGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AGGRSAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GSGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AGGRTAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GTGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AGGRVAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GVGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AGGRYAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GYGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AGHRGAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGHR + GAGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AGHRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AGIRGAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGIR + GAGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AGIRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AGKRGAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGKR + GAGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AGKRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AGLRGAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGLR + GAGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AGLRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AGMRGAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGMR + GAGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AGMRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AGNRGAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGNR + GAGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AGNRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AGQRGAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGQR + GAGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AGQRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AGSRGAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGSR + GAGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AGSRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AGTRGAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGTR + GAGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AGTRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AGVRGAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGVR + GAGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AGVRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AGYRGAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGYR + GAGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AGYRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AHGRGAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AHGR + GAGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AHGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AIGRGAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AIGR + GAGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AIGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AKGRGAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AKGR + GAGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AKGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-ALGRGAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-ALGR + GAGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-ALGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AMGRGAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AMGR + GAGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AMGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-ANGRGAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-ANGR + GAGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-ANGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AQGRGAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AQGR + GAGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AQGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-ASGRGAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-ASGR + GAGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-ASGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-ATGRGAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-ATGR + GAGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-ATGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AVGRGAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AVGR + GAGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AVGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AYGRGAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AYGR + GAGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AYGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
ovalbumin + H2O
?
show the reaction diagram
P00775
-
-
-
?
p-amidinophenyl acetate + H2O
p-amidinophenol + acetate
show the reaction diagram
-
-
-
-
?
p-nitrophenyl acetate + H2O
p-nitrophenol + acetate
show the reaction diagram
-
-
-
-
?
p-nitrophenyl p'-guanidinobenzoate + H2O
?
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-p'-guanidino benzoate + H2O
p-nitrophenol + p-guanidinobenzoate
show the reaction diagram
-
-
-
?
p-nitrophenyl-p'-guanidino benzoate + H2O
p-nitrophenol + p-guanidinobenzoate
show the reaction diagram
-
beta-trypsin is more reactive than alpha-trypsin
-
-
?
p-tosyl-L-Arg methyl ester + H2O
?
show the reaction diagram
-
-
-
-
?
p-tosyl-L-Arg methyl ester + H2O
?
show the reaction diagram
-
-
-
-
?
p-tosyl-L-Arg methyl ester + H2O
?
show the reaction diagram
Cambarus affinis
-
-
-
-
?
Pefachrome + H2O
?
show the reaction diagram
-
-
-
?
Pefachrome tPA + H2O
?
show the reaction diagram
-
-
-
?
peroxidase + H2O
?
show the reaction diagram
P00775
-
-
-
?
pre-Pro-Arg-insulin + H2O
mono/di-Arg insulin + ?
show the reaction diagram
-
-
-
-
-
Pro-Arg-insulin + H2O
mono/di-Arg insulin + ?
show the reaction diagram
-
-
-
-
-
pro-human defensin-5 + H2O
human defensin-5 + pro-human defensin-5 propeptide
show the reaction diagram
-
pro-human defensin-5 in the intestinal lumen is processed by trypsin in a complex in which chymotrypsinogen is also cleaved for activation
-
-
?
pro-insulin + H2O
human insulin-threonine-ester
show the reaction diagram
-
-
-
-
-
pro-matrix metalloproteinase-2 + H2O
matrix metalloproteinase-2 + pro-peptide
show the reaction diagram
-
activation by trypsin depends on various factors such as the level of exogenously added Ca2+ and Brij-35, temperature, and trypsin concentration. Activation occurs as sequential processing, initially generating an active 62 kDa species followed by successive truncation of the C-terminal domain leading to active species of 56 kDa, 52 kDa and 50kDa. Comparison with activation of pro-matrix metalloproteinase-2 by membrane-type 1 metalloproteinase or 4-aminophenylmercuric acetate
-
-
?
protease-activated receptor-1 + H2O
?
show the reaction diagram
-
activation
-
-
?
protease-activated receptor-2 + H2O
?
show the reaction diagram
-
activation
-
-
?
protease-activated receptor-3 + H2O
?
show the reaction diagram
-
activation
-
-
?
protease-activated receptor-4 + H2O
?
show the reaction diagram
-
activation
-
-
?
proteinase-activated receptor 1 + H2O
?
show the reaction diagram
-
-
-
-
?
proteinase-activated receptor PAR-1 + H2O
activated proteinase-activated receptor PAR-1
show the reaction diagram
-
no activation by mesotrypsin in epithelial cells, but mesotrypsin activates PAR-1 in astrocytoma cells. Half-maximal response for both cationic and anionic trypsin at about 10-20 nM
-
-
?
proteinase-activated receptor PAR-2 + H2O
activated proteinase-activated receptor PAR-2
show the reaction diagram
-
no activation by mesotrypsin in epithelial cells. Half-maximal response for both cationic and anionic trypsin at about 5 nM
-
-
?
proteinase-activated receptor PAR-3 + H2O
activated proteinase-activated receptor PAR-3
show the reaction diagram
-
no activation by mesotrypsin in epithelial cells. Half-maximal response for both cationic and anionic trypsin at about 10-20 nM
-
-
?
S-2222 + H2O
?
show the reaction diagram
-
-
-
-
?
succinyl-Ala-Ala-Pro-Arg-7-amido-4-methylcoumarin + H2O
succinyl-Ala-Ala-Pro-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
succinyl-Ala-Ala-Pro-Arg-p-nitroanilide + H2O
?
show the reaction diagram
-
-
-
-
?
succinyl-Ala-Ala-Pro-Leu-7-amido-4-methylcoumarin + H2O
succinyl-Ala-Ala-Pro-Leu + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
succinyl-Ala-Ala-Pro-Lys-7-amido-4-methylcoumarin + H2O
succinyl-Ala-Ala-Pro-Lys + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
succinyl-Ala-Ala-Pro-Lys-p-nitroanilide + H2O
?
show the reaction diagram
-
-
-
-
?
succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin + H2O
succinyl-Ala-Ala-Pro-Phe + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
succinyl-Ala-Ala-Pro-Trp-7-amido-4-methylcoumarin + H2O
succinyl-Ala-Ala-Pro-Trp + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
succinyl-Ala-Ala-Pro-Tyr-7-amido-4-methylcoumarin + H2O
succinyl-Ala-Ala-Pro-Tyr + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
succinyl-L-Ala-L-Ala-L-Pro-L-Leu-7-amido-4-methylcoumarin + H2O
succinyl-L-Ala-L-Ala-L-Pro-L-Lys + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
superoxide dismutase 1 + H2O
?
show the reaction diagram
-
-
-
-
?
tert-butoxycarbonyl-L-Gln-L-Ala-L-Arg-7-amido-4-methylcoumarin + H2O
tert-butoxycarbonyl-L-Gln-L-Ala-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
tert-butyloxycarbonyl-Gln-Ala-Arg-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
-
-
-
?
tert-butyloxycarbonyl-Gln-Arg-Arg-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
-
-
-
?
tert-butyloxycarbonyl-Gln-Gly-Arg-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
-
-
-
?
tert-butyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
-
-
-
?
Tos-L-Arg-OMe + H2O
?
show the reaction diagram
-
-
-
-
?
tosyl-Gly-Pro-Arg-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
-
-
-
?
tosyl-Gly-Pro-Arg-4-nitroanilide + H2O
?
show the reaction diagram
Bos taurus, salmon
-
-
-
?
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
P00775, -
-
-
?
tosyl-Gly-Pro-Lys-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
-
-
-
?
tosyl-Gly-Pro-Lys-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
P00775, -
-
-
?
tosyl-GPK-p-nitroanilide + H2O
tosyl-GPK + p-nitroaniline
show the reaction diagram
-
-
-
-
?
tosyl-GPR-p-nitroanilide + H2O
tosyl-GPR + p-nitroaniline
show the reaction diagram
-
-
-
-
?
ubiquitin + H2O
?
show the reaction diagram
-
-
-
-
?
VP4 + H2O
fragments VP8* and VP5*
show the reaction diagram
-
outer layer protein of rotavirus capsid
-
-
?
Z-Ala-Arg-Arg-7-amido-4-methylcoumarin + H2O
Z-Ala-Arg-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
low amidolytic activity
-
-
?
Z-Arg-7-amido-4-methylcoumarin + H2O
Z-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
Z-Arg-7-amido-4-methylcoumarin + H2O
Z-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
low amidolytic activity
-
-
?
Z-Arg-Arg-7-amido-4-methylcoumarin + H2O
Z-Arg-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
low amidolytic activity
-
-
?
Z-Gly-Gly-Arg-7-amido-4-methylcoumarin + H2O
Z-Gly-Gly-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
low amidolytic activity
-
-
?
Z-Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O
Z-Gly-Pro-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
Z-Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O
Z-Gly-Pro-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
high amidolytic activity
-
-
?
Z-Gly-Pro-Arg-p-nitroanilide + H2O
Z-Gly-Pro-Arg + p-nitroaniline
show the reaction diagram
-
-
-
-
?
Z-Phe-Arg-4-methylcoumaryl-7-amide + H2O
Z-Phe-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
low activity
-
-
?
Z-Phe-Arg-7-amido-4-methylcoumarin + H2O
Z-Phe-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
high amidolytic activity
-
-
?
MeOSuc-Ala-Arg 4-nitroanilide + H2O
MeOSuc-Ala-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
the active centre is the catalytic triad: His57, Ser195, Asp102
-
-
-
additional information
?
-
-
Asp189 is responsible for the specificity of the enzyme
-
-
-
additional information
?
-
-, O18434, Q9NB82
very low activity with dietary protein and azocasein
-
?
additional information
?
-
-
capable of agglutinating both bloodstream-form and procyclic trypanosomes as well as rabbit red blood cells
-
-
-
additional information
?
-
-
trypsin treatment alters a number of characteristics of KATP channel pharmacology, this may be due to action at possibly more than one site but includes the functional cleavage of the sulfonylurea receptor from the KATP channel
-
-
-
additional information
?
-
-
enzyme pancreatic isoforms cationic trypsin and anionic trypsin show activity on human proteinase-activated receptors PAR-1, PAR-2, PAR-3 comparable to that of bovine pancreatic trypsin. Mesotrypsin cannot activate or disable proteinase-activated receptors in human epithelial cells, but activates PAR-1 in human astrocytoma cells
-
-
-
additional information
?
-
-
no substrate: type II collagen
-
-
-
additional information
?
-
-
strong interaction of Phe in position P4 with enzyme
-
-
-
additional information
?
-
-
does not hydrolyze o-aminobenzoyl-AHGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) and o-aminobenzoyl-ATGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
additional information
?
-
-
does not hydrolyze o-aminobenzoyl-ASGRGAGQ-(2,4-dinitrophenyl-ethylene diamine), o-aminobenzoyl-ADGRGAGQ-(2,4-dinitrophenyl-ethylene diamine), o-aminobenzoyl-AYGRGAGQ-(2,4-dinitrophenyl-ethylene diamine), o-aminobenzoyl-ANGRGAGQ-(2,4-dinitrophenyl-ethylene diamine), o-aminobenzoyl-AHGRGAGQ-(2,4-dinitrophenyl-ethylene diamine), and o-aminobenzoyl-ATGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
additional information
?
-
-
does not react with proteinase-activated receptor 2
-
-
-
additional information
?
-
-
exhibits a relatively high specificity for Lys/Arg-Ser/Thr peptide bonds
-
-
-
additional information
?
-
-
active 1918 pandemic flu viral neuraminidase is resistant to trypsin digestion
-
-
-
additional information
?
-
-
does not cleave enhanced green fluorescent protein-T1m and wild type enhanced green fluorescent protein
-
-
-
additional information
?
-
-
salmon trypsin induces interleukin-8 production in human airway epithelial cells, purified salmon trypsin activates nuclear factor-kappa B-mediated gene expression in HeLa cells overexpressing human protease-activated receptor-2, the production of interleukin-8 by salmon trypsin is mediated by protease-activated receptor-2
-
-
-
additional information
?
-
-
the sequence of trypsin is IVGGY
-
-
-
additional information
?
-
-
trypsin is involved in the progesterone-induced acrosome reaction in canine spermatozoa
-
-
-
additional information
?
-
-
trypsin treatment increases zone of polarizing activity signaling of non-zone of polarizing activity tissue
-
-
-
additional information
?
-
-
does not hydrolyse D-Tat
-
-
-
additional information
?
-
-
cleavage of the potentially inhibitory peptides by isozyme trypsin-3, substrate specificity, overview
-
-
-
additional information
?
-
-
identification of autolytic cleavage sites within the trypsin I molecule, overview
-
-
-
additional information
?
-
-
the enzyme from catayzes also the reverse reaction and synthesizes Nalpha-(tert-butoxycarbonyl)-L-alanyl-L-alanine-4-nitroanilide from Nalpha-(tert-butoxycarbonyl)-L-alanine-4-guanidinophenyl ester as acyl donor and L-alanine-p-nitroanilide (L-Ala-pNA) as acyl acceptor, overview
-
-
-
additional information
?
-
-
the purified trypsin from Colossoma macropomum shows substrate specificity for arginine at P1, efficiently hydrolizing substrates with leucine and lysine at P2 and serine and arginine at P10
-
-
-
additional information
?
-
B3IYE4
trypsin specifically cleaves the peptide bond on the carboxyl side of Lys and Arg residues. It is characterized by a common catalytic mechanism involving the catalytic triad of three essential amino acid residues His57, Asp102, and Ser195 and a substrate determinant residue Asp189
-
-
-
additional information
?
-
-
trypsin-catalyzed peptide synthesis with inverse substrates as acyl donor components, overview
-
-
-
additional information
?
-
-
Arg-4-methylcoumaryl-7-amide and Suc-Leu-Leu-Val-Lys-4-methylcoumaryl-7-amide are poor substrates, substrate specificities of trypsin A and B, overview
-
-
-
additional information
?
-
-
three-dimensional structure and topological specificities, substrate recognition in which residue 71 plays a crucial role, overview. Identification of amino acid residue(s) related to collagen binding of wild-type and mutant enzymes
-
-
-
additional information
?
-
-
three-dimensional structure and topological specificities, substrate recognition, overview. The neighboring residues Leu71 and Gln72 in the N-terminal beta-barrel domain of the enzyme synergistically play an important role in substrate recognition. Identification of amino acid residue(s) related to collagen binding for wild-type and mutant enzymes
-
-
-
additional information
?
-
-
trypsin shows protease and esterase activities
-
-
-
additional information
?
-
-
trypsin-catalyzed peptide synthesis using 4-amidino- and 4-guanidinophenyl esters of Nalpha-tert-butyloxycarbonyl-amino acid as acyl donor components with two types of acyl acceptors, overview
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
DrmSP8-36 + H2O
?
show the reaction diagram
-
trypsin is the major endopeptidase regulating N-terminal truncated sex peptide DrmSP8-36 in hemolymph, the trypsin cleavage site R7-K8 is essential for release of the C-terminal fragment SP8-36 from sperm tail, the protein is cleaved more rapidly and completely in mated female hemolymph
-
-
?
enhanced green fluorescent protein-T1 + H2O
?
show the reaction diagram
-
enhanced green fluorescent protein-T1 is specifically cleaved into two major fragments by trypsin at the grafted cleavage site, approximately 20 and 8 kDa
-
-
?
enhanced green fluorescent protein-T2 + H2O
?
show the reaction diagram
-
enhanced green fluorescent protein-T2 is specifically cleaved into two major fragments by trypsin at the grafted cleavage site, approximately 18 and 10 kDa
-
-
?
Gelatin + H2O
?
show the reaction diagram
-
-
-
-
?
human cartilage collagen type II + H2O
?
show the reaction diagram
-
use of enzyme and its regulators as markers of prognosis and disease activity in rheumatoid arthritis
-
-
?
protease-activated receptor-1 + H2O
?
show the reaction diagram
-
activation
-
-
?
protease-activated receptor-2 + H2O
?
show the reaction diagram
-
activation
-
-
?
protease-activated receptor-3 + H2O
?
show the reaction diagram
-
activation
-
-
?
protease-activated receptor-4 + H2O
?
show the reaction diagram
-
activation
-
-
?
intestinal alkaline sphingomyelinase bound to mucosa + H2O
free intestinal alkaline sphingomyelinase
show the reaction diagram
-
cleavage at R440, first tryptic site upstream of signal anchor. Physiological role of enzyme in sphingomyelin digestion
-
-
?
additional information
?
-
-
capable of agglutinating both bloodstream-form and procyclic trypanosomes as well as rabbit red blood cells
-
-
-
additional information
?
-
-
trypsin treatment alters a number of characteristics of KATP channel pharmacology, this may be due to action at possibly more than one site but includes the functional cleavage of the sulfonylurea receptor from the KATP channel
-
-
-
additional information
?
-
-
enzyme pancreatic isoforms cationic trypsin and anionic trypsin show activity on human proteinase-activated receptors PAR-1, PAR-2, PAR-3 comparable to that of bovine pancreatic trypsin. Mesotrypsin cannot activate or disable proteinase-activated receptors in human epithelial cells, but activates PAR-1 in human astrocytoma cells
-
-
-
additional information
?
-
-
active 1918 pandemic flu viral neuraminidase is resistant to trypsin digestion
-
-
-
additional information
?
-
-
does not cleave enhanced green fluorescent protein-T1m and wild type enhanced green fluorescent protein
-
-
-
additional information
?
-
-
salmon trypsin induces interleukin-8 production in human airway epithelial cells, purified salmon trypsin activates nuclear factor-kappa B-mediated gene expression in HeLa cells overexpressing human protease-activated receptor-2, the production of interleukin-8 by salmon trypsin is mediated by protease-activated receptor-2
-
-
-
additional information
?
-
-
the sequence of trypsin is IVGGY
-
-
-
additional information
?
-
-
trypsin is involved in the progesterone-induced acrosome reaction in canine spermatozoa
-
-
-
additional information
?
-
-
trypsin treatment increases zone of polarizing activity signaling of non-zone of polarizing activity tissue
-
-
-
additional information
?
-
B3IYE4
trypsin specifically cleaves the peptide bond on the carboxyl side of Lys and Arg residues. It is characterized by a common catalytic mechanism involving the catalytic triad of three essential amino acid residues His57, Asp102, and Ser195 and a substrate determinant residue Asp189
-
-
-
additional information
?
-
-
trypsin-catalyzed peptide synthesis with inverse substrates as acyl donor components, overview
-
-
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Ca2+
-
highest activity in presence of 30-50 mM, isoforms A, B, C
Ca2+
-
stabilization of enzyme
Ca2+
-
required for thermal stability
Ca2+
-
activates at concentrations above 0.4 mM
Ca2+
-
stabilized in the presence of 2 mM Ca2+
Ca2+
-
10 mM Ca2+ have a stabilizing effect
Ca2+
-
2 mM, stabilizing effect
Ca2+
-
-
Ca2+
-
activates, best at 20 mM
Ca2+
-
stabilizes the enzyme
Ca2+
-
stabilizes the purified enzyme
Ca2+
B3IYE4
required, amino acid residues Glu70, Asn72, Glu77, and Glu80 are conserved in the calcium-binding loop
Ca2+
D0G7G2
required
Ca2+
C4TP29
required
Na+
-
Na+ activation is interwoven with substrate selectivity in the trypsin scaffold
Ni2+
-
trypsin N143H/E151H hydrolyzes the peptide AGPYAHSS exclusively in presence of Ni2+ or Zn2+ with high levels of catalytic efficiency
Zn2+
-
trypsin N143H/E151H hydrolyzes the peptide AGPYAHSS exclusively in presence of Ni2+ or Zn2+ with high levels of catalytic efficiency
Ca2+
-
activates, plays an important role in trypsin stabilization
additional information
-
no activation by Ca2+
additional information
-
not dependent on Ca2+ for activation or stability
additional information
-
Ca2+, Mg2+, K+, and Na+ do not affect the enzyme's proteolytic activity
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
(2R,4R)-4-phenyl-1-[Nalpha-(7-methoxy-2-naphthalenesulfonyl)-L-arginyl]-2-piperidinecarboxylic acid
-
competitive, small molecular weight inhibitor without tryptase inhibitor activity
(4-(6-chloro-naphthalene-2-sulfonyl)-piperazin-1-yl)-(3,4,5-6-tetrahydro[2H-1,4']bipyridinyl-4yl)-methanone
-
-
1,10-phenanthroline
-
-
1-(L-trans-epoxysuccinyl-leucylamino)-4-guanidinobutane
-
6.09% inhibition at 0.1 mM
1-(L-trans-epoxysuccinyl-leucylamino)-4-guanidinobutane
-
16.9% inhibition at 0.1 mM
1-[N2-[(7-methoxynaphthalen-2-yl)sulfonyl]-L-arginyl]-4-phenylpiperidine-2-carboxylic acid
-
IC50: 0.0003 mM
2,7-bis(4-amidinobenzylidene)-cycloheptan-1-one
-
-
2,7-bis(4-amidinobenzylidene)cycloheptan-1-one
-
unspecific strong inhibition
2-mercaptoethanol
-
0.1 mM, isoenzyme aT-I loses 90% of its activity, isoenzyme aT-II loses 93% of its activity
3,4-Dichloroisocoumarin
-
-
4-(2-aminoethyl)-benzensulfonylfluoride
-
isozyme TryIII activity is completely (more than 95%) inhibited by 4-(2-aminoethyl)-benzensulfonylfluoride
4-(amidinophenyl)methanesulfonyl fluoride
-
-
6-amidino-2-naphthol
-
mixed-type inhibition
acetonitrile
-
partial inhibition at 20% acetonitrile, a 5% aqueous acetonitrile solution less likely reduces the activity of the porous polymer monolith-immobilized trypsin
acyclic SFTI
-
i.e. oSFTI, modified sunflower trypsin inhibitor 1, SFTI-1, generated by Fmoc-based automated synthesis
acyclic sun flower trypsin inhibitor-1
-
-
-
Adenanthera pavonina trypsin inhibitor
-
i.e. ApTI, isolated and purified from seeds, retards larval and pupal developmental and growth of larvae of Anticarsia gemmatalis, overview
-
Adenanthera pavonina trypsin inhibitor
-
i.e. ApTI, isolated and purified from seeds, retards larval and pupal developmental and growth of Corcyra cephalonica, overview
-
Adenanthera pavonina trypsin inhibitor
-
i.e. ApTI, isolated and purified from seeds, retards larval and pupal developmental and growth of Diatraea saccharalis, overview
-
Adenanthera pavonina trypsin inhibitor
-
i.e. ApTI, isolated and purified from seeds, retards larval and pupal developmental and growth of Anagasta kuehniella, a severe pest in flour mills, at 1% with a significant reduction in larval survival and weight, effects of this inhibitor on food consumption, absorption and utilization, as well as the effects of the inhibitor on the midgut proteolytic activity of larvae fed on an artificial diet, overview
-
Adenanthera pavonina trypsin inhibitor
-
i.e. ApTI, isolated and purified from seeds, has only slight retarding effect on larval and pupal developmental and growth of Sitotroga cerealella, overview
-
Adenanthera pavonina trypsin inhibitor
-
i.e. ApTI, isolated and purified from seeds, retards larval and pupal developmental and growth of Spodoptera frugiperda, overview
-
alpha-1-antitrypsin
-
in gestational diabetes mellitus reduction of serum trypsin inhibitory capacity may be due to non-enzymatic glycosylation of alpha-1-antitrypsin or oxidation of methionine in the active site of alpha-1-antitrypsin
-
Alpha1-antitrypsin
-
-
-
alpha1-antitrypsin Pittsburgh
-
effective inhibitor, variant of alpha1-antitrypsin containing an Arg residue in place of the P1 Met358 in the reactive-site peptide bond
-
antipain
-
IC50: 0.04 mM
antipain
-
0.01 mM, isoenzyme aT-I loses 96% of its activity, isoenzyme aT-II loses 99% of its activity
Apios Americana trypsin inhibitor
-
AATI, strong inhibitor, complete inhibition with Apios Americana trypsin inhibitor bound to trypsin in a 1:2 molar ratio
-
Aprotinin
-
-
Aprotinin
-
very efficient inhibition of trypsin-1 and trypsin-2, but not of trypsin-4
Aprotinin
-
-
Aprotinin
-
immobilized enzyme
atropine
-
strongly diminishes trypsin activity in stressed animals
AVNIPFKVHFRCKAAFC
-
a small trypsin inhibitor from the skin secretion of the frog Odorrana grahami, only inhibits the hydrolysis activity of trypsin on synthetic chromogenic substrate
Ba2+
-
27% inhibition at 5 mM
Bauhinia variegata var. variegata trypsin inhibitor
-
i.e. BvvTI, a Kunitz-type inhibitor from seeds of the Camels foot tree, Bauhinia variegata var. variegata. The inhibitor is also capable of significant inhibition of the proliferation of nasopharyngeal cancer CNE-1 cells in a selective way and of inhibiting anti-HIV-1 reverse transcriptase activity, overview
-
Benzamidine
-
unspecific, weak inhibition
Benzamidine
-
1 mM, isoenzyme aT-I loses 90% of its activity, isoenzyme aT-II loses 93% of its activity
Benzamidine
-
complete inhibition
Benzamidine
-
5 mM, 22% residual activity
Benzamidine
-
competitive inhibitor
Benzamidine
-
99% inhibition at 1 mM
Benzamidine
-
55% inhibition at 5 mM
Benzamidine
-
54% inhibition at 5 mM
Benzamidine
-
72.0% inhibition of trypsin A, 95.4% of trypsin B, at 1 mM. 88.8% inhibition of trypsin A, 97.6% of trypsin B at 0.1 mM,
Benzamidine
-
-
Benzamidine
-
-
benzylamine
Bos taurus, salmon
-
-
benzyloxycarbonyl-amino(4-guanidinophenyl)methyl-bis(4-ethylphenyl)phosphonate
-
50% inhibition at 0.000017 mM, comparison with inhibitory effect on urokinase
benzyloxycarbonyl-amino(4-guanidinophenyl)methyl-bis(4-isopropylphenyl)phosphonate
-
50% inhibition at 0.000061 mM, comparison with inhibitory effect on urokinase
benzyloxycarbonyl-amino(4-guanidinophenyl)methyl-bis(4-methylthiophenyl)phosphonate
-
50% inhibition at 0.0000087 mM, comparison with inhibitory effect on urokinase
benzyloxycarbonyl-amino(4-guanidinophenyl)methyl-bis(4-t-butylphenyl)phosphonate
-
50% inhibition at 0.000088 mM, comparison with inhibitory effect on urokinase
bis(m-amidinosalicylidene-L-alaninato)iron(III)
-
-
bis(p-amidinosalicylidene-L-alaninato)iron(III)
-
-
-
black gram trypsin inhibitor 1
-
highly effective trypsin inhibitor, 87.5% of trypsin inhibitory activity of black gram trypsin inhibitor 1 is retained after treatment with 10 mM dithiothreitol for 2 hours, and the activity dwindles to 12.5% after treatment with 100 mM dithiothreitol for 2 hours
-
black gram trypsin inhibitor 2
-
highly effective trypsin inhibitor, the trypsin inhibitory activity of black gram trypsin inhibitor 2 is unaffected after exposure to 100 mM dithiothreitol for 2 hours
-
black gram trypsin inhibitor 3
-
highly effective trypsin inhibitor, in the presence of 10 mM dithiothreitol trypsin inhibitory of black gram trypsin inhibitor 3 drops to 57.1% after 1 h and becomes undetectable after 2 hours, while treatment of 100 mM dithiothreitol for 1 h has the same effect
-
Bovine pancreatic trypsin inhibitor
-
BPTI, a natural non-specific serine protease inhibitor which possesses the ability to inhibit trypsin, the inhibitory activity of recombinant BPTI against trypsin is the same as natural BPTI, the trade name is aprotinin
-
Bovine pancreatic trypsin inhibitor
-
BPTI
-
Bovine pancreatic trypsin inhibitor
-
also known as aprotinin. At the lowest aprotinin concentration of 0.000625 mg, the residual trypsin obtained in neonate larvae of shoot borer, internode borer and top borer is 33.6, 24.8 and 9.3%, respectively. The residual trypsin for third instar of shoot borer, internode borer and top borer at the lowest aprotinin concentration of 0.000125 mg is 55.0, 65.0 and 20.7%, respectively. The amount of aprotinin needed to bring about 50% inhibition of gut proteinases of third instar larvae is 0.00025 mg for shoot borer and 0.0025 mg for internode borer whereas 0.000125 mg is sufficient to bring about nearly 80% inhibition in top borer.
-
Bovine pancreatic trypsin inhibitor
-
-
-
Bovine pancreatic trypsin inhibitor
-
i.e. BPTI or aprotinin, influence of temperature on the relationship between structure and dynamics of the inhibitor protein, calculations and X-ray crystal structure of BPTI at 1.5 A resolution, global diffusion and internal motions, overview
-
bovine secretory inhibitors
-
A and B
-
Bowman-Birk inhibitor
-
double-headed native trypsin/chymotrypsin inhibitor in soybeans, which inhibits trypsin and chymotrypsin in a non-competitive manner, 78% reduced activity in the presence of 0.0005 mM Bowman-Birk inhibitor
Bowman-Birk inhibitor
-
-
BPTI-20st
-
simplified variant of bovine pancreatic trypsin inhibitor containing 20 alanines, retains a wild type level of trypsin inhibitory activity
-
BPTI-[5,55]st
-
single-disulfide-bonded variant of bovine pancreatic trypsin inhibitor, retains a wild type level of trypsin inhibitory activity
-
Captopril
-
feeding angiotensin converting enzyme inhibitor captopril down-regulates trypsin activity in the larval gut, injecting third instar larvae with a combination of Aedes aegypti trypsin-modulating oostatic factor and captopril downregulates trypsin biosynthesis in the larval gut
chymostatin
-
-
chymostatin
-
-
Clitocybe nebularis trypsin inhibitor
-
CnSPI, several inhibitor variants, separation by trypsin affinity chromatography. Possible regulatory role for CnSPIs in the endogenous proteolytic system of Clitocybe nebularis. Isolation from frozen fruiting bodies or basidiocarps
-
cMCoTI-II
-
-
-
cnispin
-
a Clitocybe nebularis trypsin inhibitor variant, recombinantly expressed in Escherichia coli, highly specific towards trypsin, fast-acting, and tight-binding. Molecular cloning of the gene and cDNA encoding cnispin, overview
-
Co2+
-
48% inhibition at 5 mM
Cu2+
-
14% inhibition at 5 mM
Cu2+
-
68% inhibition at 5 mM
cyclic SFTI
-
i.e. cSFTI, modified sunflower trypsin inhibitor 1, SFTI-1, generated by Fmoc-based automated synthesis
DMTI-II
-
trypsin inhibitor from seeds of Dimorphandra mollis. The inhibitory activity is stable over a wide range and in the presence of DTT
-
domesticated soybean inhibitor
-
SBTI
-
DOTA-SFTI
-
modified sunflower trypsin inhibitor 1, SFTI-1, labeled, generated by Fmoc-based automated synthesis
E-64
-
18.3% inhibition of trypsin A, 11.1% of trypsin B, at 0.001 mM. 46.9% inhibition of trypsin A, 33.8% of trypsin B at 0.01 mM
E64
-
weak inhibition
Ecballium elaterium trypsin inhibitor II
-
EETI, a a model member of the knottin family, used for construction of libraries of EETI loop-substituted variants with diversity in both amino acid sequence and loop length, wild-type EETI or loop-substituted clones expressed from yeast, structure-function analysis, modelling, overview
-
EDTA
-
50 mM, 88% residual activity
EDTA
-
6.85% inhibition at 2 mM
EDTA
-
weak inhibition, 18% inhibition at 10 mM
EDTA
-
12% inhibition at 5 mM
EDTA
-
10% inhibition at 2 mM
EDTA
-
a metalloenzyme inhibitor, 8% inhibition at 5 mM
EDTA
-
14.2% inhibition at 2 mM
enalapril
-
feeding angiotensin converting enzyme inhibitor enalapril down-regulates trypsin activity in the larval gut
ethylenediaminetetraacetic acid
-
partial
Gly-L-Arg-(2-amino butanoyl)-L-Thr-Nphe-L-Ser-L-Ile-L-Pro-L-Pro-L-Ile-(2-amino butanoyl)-L-Phe-L-Pro-L-Asp
-
-
Gly-L-Arg-L-Cys-L-Thr-L-Lys-L-Ser-L-Ile-L-Pro-L-Pro-L-Ile-L-Cys-L-Phe-L-Pro-L-Asp
-
sun flower trypsin inhibitor SFTI-1
Glycine max trypsin inhibitor
-
from raw soymilk, inhibition kinetics, overview
-
Hg2+
-
72% inhibition at 5 mM
Hg2+
-
57% inhibition at 5 mM
Hg2+
-
strong inhibition
I-ovalbumin
-
high affinity interaction of enzyme with I-ovalbumin, the product of a heating transition of ovalbumin which acts as a potent reversible serine proteinase inhibitor. Interaction is characterized by high kinetic association constants and low kinetic dissociation konstants
-
iodoacetic acid
-
16.5% inhibition at 1 mM
Kunitz domain 1 of tissue factor pathway inhibitor-2
-
-
-
Kunitz type trypsin inhibitor from Glycine max
-
isolated and purified from Korean large black soybeans cultivar, overview. Inhibits bovine trypsin, but also shows Anti-HIV reverse transcriptase activity, cytokine-inducing activity and antiproliferative activity on tumor cell lines
-
L-1-chloro-3-tosylamido-4-phenyl-2-butanone
-
-
L-1-chloro-3-[4-tosylamido]-4-phenyl-2-butanone
-
-
L-Lys-L-Cys-L-Thr-Nlys-L-Ser-L-Ile-L-Pro-L-Pro-L-Ile-L-Cys-L-Phe-L-Pro-L-Asp
-
-
leech-derived tryptase inhibitor
-
i.e. LDTI, purely competitive inhibitor
-
LEKTI
-
the LEKI gene encodes a 15-domain serine proteinase inhibitor, disulfide bonds are important for LEKTI function, noncompetitive mechanism, expression and purification of human LEKTI gene in Sf9
Leupeptin
-
IC50: 0.0002 mM
Leupeptin
-
0.1 mM, isoenzyme aT-I loses 97% of its activity, isoenzyme aT-II completely loses its activity
Leupeptin
-
-
Leupeptin
-
-
Leupeptin
-
-
lily bulb trypsin inhibitor
-
-
-
Lima bean trypsin inhibitor
-
-
-
LKGCWTKSIPPKPCFGK
-
-
Locusta migratoria protease inhibitor 1
-
strong inhibition
-
Locusta migratoria protease inhibitor 3
-
strong inhibition, the inhibitor interacts with trypsin through the reactive site P3-P4' and the P10-P6 residues
-
low-molecular-mass trypsin inhibitor inhibitor type-2
-
from Sinapis alba, inhibitor consists of a peptide mixture, displaying Ile or Arg at position 43, Trp or kynurenine at position 44, and C-terminal ragged ends
-
m-amidinosalicylidene-L-alaninato(aqua)copper(II)
-
-
m-amidinosalicylidene-L-alaninato(aqua)copper(II) hydrochloride
-
-
-
m-guanidinosalicylidene-L-alaninato(aqua)copper(II)
-
-
Mg2+
-
24% inhibition at 5 mM
Mn2+
-
34.5% inhibition at 5 mM
mung bean trypsin inhibitor
-
synthesis and inhibitory activity of mutant inhibitor variants expressed in Escherichia coli, overview
-
mustard trypsin inhibitor 2
-
-
-
mustard trypsin inhibitor 2
-
80% of trypsin activity can be inhibited by 42 nM mustard trypsin inhibitor 2
-
mustard trypsin inhibitor II
O18434, Q9NB82
inhibition of HzTrypsin-C, no inhibition of HzTrypsin-S
-
N-4-tosyl-L-lysine chloromethyl ketone
-
82.9% inhibition at 5 mM
-
N-alpha-tosyl-L-Lys-chloromethyl ketone
-
complete inhibition at 1 mM
N-alpha-tosyl-L-lysine chlormethylketone
-
less than 50% inhibition of isozyme TryIII by the trypsin-specific inhibitor N-alpha-tosyl-L-lysine chlormethylketone
N-alpha-tosyl-L-lysine chloromethyl ketone
-
-
N-alpha-tosyl-L-lysine-chloromethylketone
-
complete inhibition at 5 mM
N-p-tosyl-L-lysine chloromethyl ketone
-
complete inhibition at 5 mM
N-p-tosyl-L-lysine chloromethyl ketone
-
89% inhibition at 0.1 mM
N-p-tosyl-L-lysine chloromethyl ketone
-
88% inhibition at 5 mM
N-p-tosyl-lysine chloroketone
-
-
N-p-tosyl-lysine chloroketone
-
-
N-p-tosyl-lysine chloroketone
-
-
N-tosyl-L-lysine-chloromethyl ketone
-
complete inhibition
N-tosyl-L-lysine-chloromethyl ketone
-
82% inhibition at 5 mM
N-tosyl-L-phenyl-alanine-chloromethyl ketone
-
11% inhibition at 1mg/ml
N-tosyl-L-phenylalanine-chloromethyl ketone
-
5.34% inhibition at 5 mM
Na-p-tosyl-L-lysine chloromethyl ketone hydrochloride
-
inhibits trypsin specifically and irreversibly, 40% reduced activity in the presence of 1 mM Na-p-tosyl-L-lysine chloromethyl ketone hydrochloride
NaCl
-
activity decreases with increasing NaCl concentrations (0-30%)
NaCl
-
exhibits a progressive decrease in activity with increasing NaCl concentration (0-30%)
NaCl
-
60& inhibition at 35% NaCl
NaCl
-
60% inhibition at 30% NaCl
NaCl
-
enzyme activity continuously decreases with increasing NaCl concentration of 0-30%
NaCl
-
enzyme activity continuously decreases to 50% of maximal activity with increasing NaCl concentration of 0-30%
NaCl
-
relative activities of trypsin isozymes A, B, and C at 15% NaCl are approximately 39%, 38% and 40% of maximal activity, respectively
NaCl
-
69% inhibition at 0.6 M
Nafamostat
-
potent inhibitor
nafamostat mesilate
-
IC50: 0.00003 mM
Nalpha-(2-naphthylsulfonyl)-L-3-amidino-phenylalanine-4'-acetyl-piperazide
-
-
Nalpha-(2-naphthylsulfonyl)-L-3-amidino-phenylalanine-4'-methylpiperidide
-
-
Nalpha-(2-naphthylsulfonyl)-L-3-amidino-phenylalanine-D-pipecolic acid
-
-
Nalpha-(2-naphthylsulfonyl)-L-3-amidino-phenylalanine-D-pipecolic acid methyl ester
-
-
Nalpha-(2-naphthylsulfonyl)-L-3-amidino-phenylalanine-isopecotinic acid
-
-
Nalpha-(2-naphthylsulfonyl)-L-3-amidino-phenylalanine-isopecotinic acid methylester
-
-
Nalpha-(2-naphthylsulfonyl)-L-3-amidino-phenylalanine-piperazide
-
-
Nalpha-p-tosyl-L-lysine chloromethyl ketone
-
complete inhibition
Nalpha-tosyl-L-Lys-chloromethyl ketone
-
91% inhibition at 1 mM
Nalpha-tosyl-L-Lys-chloromethylketone
-
-
Nalpha-tosyl-L-Lys-chloromethylketone
-
79% inhibition at 5 mM
Nalpha-tosyl-L-Lys-chloromethylketone
-
89% inhibition at 5 mM
Nalpha-tosylglycyl-3-amidino-DL-phenylalanine-methyl ester
-
-
napsagatran ethylester
-
-
nexin-1
-
nexin-1 inhibits isozymes trypsin-1 ad trypsin-2, nexin-1 inhibits trypsin-4 and forms stable complexes only with this trypsin isoenzyme
-
oMCoTI-II
-
-
-
ovomucoid
-
-
-
ovomucoid
-
-
-
ovomucoid
-
chicken ovomucoid
-
p-amidinosalicylidene-L-alaninato(aqua)copper(II)
-
-
p-nitrophenyl-p'-guanidino-benzoate
-
irreversible trypsin inhibitor
p-tosyl-L-Lys-chloromethane
-
-
p-tosyl-L-Lys-chloromethane
-
reaction towards B chain of insulin is inhibited, the activity towards the A-chain is not significantly affected
pancreatic secretory trypsin inhibitor
-
also known as serine protease inhibitor Kazal type 1
-
pancreatic secretory trypsin inhibitor
-
a Kazal-type specific trypsin inhibitor with narrow specificity for the inhibition of trypsin, with very little or no inhibitory capacity against other serine proteinases, even those exhibiting trypsin-like specificity
-
Pancreatic trypsin inhibitor
-
i.e. trasylol, kallikrein inactivator
-
Pancreatic trypsin inhibitor
-
bovine pancreatic trypsin inhibitor
-
PdKI-3.1
-
isolation of two highly pH- and thermo-stable Kunitz-type inhibitors of trypsin from seeds of the tree Pithecellobium dumosum, overview
-
PdKI-3.2
-
isolation of two highly pH- and thermo-stable Kunitz-type inhibitors of trypsin from seeds of the tree Pithecellobium dumosum, overview
-
pefabloc
-
-
Pefabloc SC
-
completely inhibits isoenzymes trypsin-1, trypsin-2, and trypsin-4
Pefabloc SC
-
complete inhibition of trypsin A and B at 1 mM
pepstatin A
-
26% inhibition at 0.01 mM
Phaseolus vulgaris trypsin inhibitor
-
two different variants of 132 kDa and 118 kDA from navy beans and red kidney beans, respectively, isolated by heat treatment and ammonium sulfate fractionation
-
phenylbutylamine
Bos taurus, salmon
-
-
Phenylethylamine
Bos taurus, salmon
-
-
phenylmethyl sulfonyl fluoride
-
-
phenylmethyl sulfonyl fluoride
-
-
phenylmethylsulfonyl fluoride
-
-
phenylmethylsulfonyl fluoride
-
-
phenylmethylsulfonyl fluoride
-
partial
phenylmethylsulfonyl fluoride
-
5 mM, 43% residual activity
phenylmethylsulfonyl fluoride
-
a serine protease inhibitor, over 90% inhibition of trypsin isozymes A, B and C at 5 mM
Phenylmethylsulfonylfluoride
-
-
Phenylmethylsulfonylfluoride
-
89.5% inhibition at 1 mM
Phenylpropylamine
Bos taurus, salmon
-
-
Plathymenia foliolosa trypsin inhibitor
-
PFTI
-
PMSF
-
0.1 mM, isoenzyme aT-I loses 89% of its activity, isoenzyme aT-II loses 80% of its activity
PMSF
-
73% inhibition at 1 mM
PMSF
-
complete inhibition
PMSF
-
62% inhibition at 1 mM, complete inhibition at 5 mM
PMSF
-
61% inhibition at 1 mM, complete inhibition at 5 mM
PMSF
-
80% inhibition at 1 mM, complete at 5 mM
PMSF
-
89% inhibition at 5 mM
PMSF
-
80.7% inhibition of trypsin A, 57.4% of trypsin B at 0.1 mM. 96.4% inhibition of trypsin A, 93.2% of trypsin B at 1 mM
polylysine
-
immobilized enzyme
porcine secretory inhibitors
-
-
-
potato inhibitor II
O18434, Q9NB82
strong inhibition of HzTrypsin-C, no inhibition of HzTrypsin-S
-
Protease Inhibitor Cocktail
-
-
-
Putranjiva roxburghii trypsin inhibitor
-
highly potent inhibitor of bovine trypsin, trypsin inhibitory activity of Putranjiva roxburghii trypsin inhibitor is completely retained up to 70C, above 70C, there is a slight decrease in the inhibitory activity retaining almost 85% inhibitory activity up to 80C, the inhibitory activity of Putranjiva roxburghii trypsin inhibitor falls sharply above 80C with a loss of almost 80% inhibitory activity at 90C, only a slight decrease of 5% in inhibitory activity is observed when Putranjiva roxburghii trypsin inhibitor is incubated for 2 h at 100 mM dithiothreitol
-
Sagittaria sagittifolia arrowhead protease inhibitor A
-
API-A
-
small glossy black soybean trypsin inhibitor
-
inhibitory activity is stable in the pH range 3-13 and in the temperature range 0-60C, is inhibited by dithiothreitol (5-25 mM) in a dose-dependent manner
-
soybean Bowman-Birk inhibitor
O18434, Q9NB82
inhibition of HzTrypsin-C,no inhibition of HzTrypsin-S
-
soybean Kunitz trypsin inhibitor
O18434, Q9NB82
strong inhibition of HzTrypsin-C, no inhibition of HzTrypsin-S
-
soybean Kunitz trypsin inhibitor
-
-
-
soybean Kunitz trypsin inhibitor
-
80% of trypsin activity can be inhibited by 42 nM soybean Kunitz trypsin inhibitor
-
Soybean trypsin inhibitor
-
IC50: 0.000009 mM
-
Soybean trypsin inhibitor
-
0.1 mg/ml, isoenzyme aT-I loses 95% of its activity, isoenzyme aT-II loses 97% of its activity
-
Soybean trypsin inhibitor
-
-
-
Soybean trypsin inhibitor
-
complete inhibition
-
Soybean trypsin inhibitor
-
0.025% w/v, 0.3% residual activity
-
Soybean trypsin inhibitor
-
strong
-
Soybean trypsin inhibitor
-
effective inhibitor
-
Soybean trypsin inhibitor
-
91% inhibition at 1mg/ml
-
Soybean trypsin inhibitor
-
84.2% inhibition at 1 mg/ml
-
Soybean trypsin inhibitor
-
isozyme TryIII activity is completely (more than 95%) inhibited by soybean trypsin inhibitor
-
Soybean trypsin inhibitor
-
96% inhibition at 1 mM
-
Soybean trypsin inhibitor
-
-
-
Soybean trypsin inhibitor
Q54137
-
-
Soybean trypsin inhibitor
-
-
-
Soybean trypsin inhibitor
-
-
-
Soybean trypsin inhibitor
-
-
-
Soybean trypsin inhibitor
-
-
-
Soybean trypsin inhibitor
-
complete inhibition
-
Soybean trypsin inhibitor
-
complete inhibition at 1 mg/ml
-
Soybean trypsin inhibitor
-
SBT, complete inhibition at 1 mg/ml
-
Soybean trypsin inhibitor
-
84% inhibition at 1 mg/ml
-
Soybean trypsin inhibitor
-
80% inhibition at 1 mg/ml
-
Soybean trypsin inhibitor
-
complete inhbition at 1 mg/ml
-
Soybean trypsin inhibitor
-
complete inhibition at 1 mg/ml
-
Soybean trypsin inhibitor
-
complete inhibition at 1 mg/ml
-
Soybean trypsin inhibitor
-
91.5% inhibition at 1 mg/ml
-
Soybean trypsin inhibitor
-
different Kunitz trypsin inhibitors, KTIs, from different soybean lines, 15 soybean experimental lines and varieties, overview
-
Soybean trypsin inhibitor
-
a Kunitz trypsin inhibitor from soybean seeds, identification of mutants, several polymorphic types that are controlled by multiple alleles, overview. Development of a single nucleotide amplified polymorphism marker for the classification of the predominant KTi types, Tia and Tib, and evaluation of KTi activities by differing KTi type total 451 soybean mutant lines, overview. Trypsin inhibitor type and trypsin inhibitor activity in TIU/mg, overview
-
Soybean trypsin inhibitor
-
a serine protease inhibitor, complete inhibition of trypsin isozymes A, B and C at 1 mg/ml
-
Soybean trypsin inhibitor
-
80.3% inhibition at 1 mg/ml
-
Soybean trypsin inhibitor
-
-
-
Spinacia oleracea trypsin inhibitor
-
high affinity between trypsin and Spinacia oleracea trypsin inhibitor
-
sun flower trypsin inhibitor-1
-
-
sunflower trypsin inhibitor 1
-
SFTI-1, binding and effects on human prostate cancer cells, overview
sunflower trypsin inhibitor-1
-
SFTI-1
sunflower trypsin inhibitor-1
-
SFTI-1, a natural 14-residue cyclic peptide, can be used for enzyme separation from other serine proteases, synthesis of SFTI-1-based supports and affinity chromatography, overview. Inhibition of trypsin by some acyclic SFTI-1 analogues, e.g. [Lys5]-SFTI-1, [Arg5]-SFTI-1, and [Phe5]-SFTI-1, no inhibition by [Leu5]-SFTI-1, overview
SVIGCWTKSIPPRPCFVK-amide
-
HV-BBI is a Bowman-Birk type protease inhibitor from the skin secretion of the Chinese Bamboo odorous frog Huia versabilis, SVIGCWTKSIPPRPCFVK-amide is a synthetic replicate of HV-BBI with the wild-type K (Lys8) residue in the presumed P1 position and is a potent, competitive, and reversible inhibitor of trypsin
SVIGCWTRSIPPRPCFVK-amide
-
HV-BBI is a Bowman-Birk type protease inhibitor from the skin secretion of the Chinese Bamboo odorous frog Huia versabilis, SVIGCWTRSIPPRPCFVK-amide is a synthetic replicate of HV-BBI with the mutant R (Arg8) residue in the presumed P1 position and is a competitive, and reversible inhibitor of trypsin with reduced potency compared to the wild type peptide SVIGCWTKSIPPRPCFVK-amide
T5E/N18R/T20G/P21S/T22D/K31M mutant Schistocerca gregaria protease (trypsin) inhibitor 1
-
the mutant inhibitor is improved compared to the wild-type inhibitor protein
-
Tamarindus indica trypsin inhibitor
-
a Kunitz-type inhibitor
-
thionine
-
from cowpea, inhibits trypsin, no inhibition of chymotrypsin
tissue inhibitor of matrix metalloproteinases-2
-
TIMP-2, prevents the C-terminal truncation of activated metalloproteinase-2, without affecting the generation of the initial 62 kDa activated species
-
tosyl-L-lysine-chloromethyl ketone
-
-
tosyl-L-Phe chloromethyl ketone
-
-
TPI-1
-
-
-
TPI-2
-
-
-
trans-epoxysuccinyl-L-leucylamido-(4-guanidino) butane
-
-
trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane
-
-
trypsin inhibitor I-S Type from soybean
-
reversible trypsin inhibitor
-
trypsin modulating oostatic factor
-
the hexapeptide NPTNLH is secreted at the last stage of vitellogenesis and inhibits the biosynthesis of trypsin in insect midgut cells
-
tumor-associated trypsin inhibitor
-
specific inhibition of degradation of gelatin and collagen type II
-
tumor-associated trypsin inhibitor
-
very efficient inhibition of trypsin-1 and trypsin-2, but not of trypsin-4
-
tumour-associated trypsin inhibitor
-
i.e. TATI, endogenous inhjibitor expressed in the cytoplasm of colon mucosa and colorectal cancer cells, shows prognostic significance of tumour-specific TATI expression in colorectal cancer, inhibitor expression is associated with a significantly decreased overall survival of colon cancer patients, and with increased liver metastasis, overview
-
ulinastatin
-
-
urinary trypsin inhibitor
-
urinary trypsin inhibitor reduces nuclear factor-kappa B activation and downregulates the expression of its related mediators, followed by the inhibition of neutrophil aggregation and infiltration in hepatic ischemiareperfusion injury
-
urinary trypsin inhibitor
-
-
-
urinary trypsin inhibitor
-
a Kunitz type protease inhibitor
-
urinary trypsin inhibitor
-
-
-
urinary trypsin inhibitor
-
also known as bikunin
-
urinary trypsin inhibitor
-
also known as ulinastatin
-
Vigna angularis trypsin inhibitor
-
from adzuki beans, 13 kDa, isolated by heat treatment and ammonium sulfate fractionation
-
Vigna mungo trypsin inhibitor
-
purification of the Bowman-Birk proteinase inhibitor from the seeds of black gram, Vigna mungo cv. TAU-1. 8041.5 Da by mass spectrometry, pI 4.3-6.0, stable up to 80C and at pH 2.0-12.0, analysis of the secondary structural conformation, overview, exhibts non-competitive-type inhibitory activity against both bovine pancreatic trypsin
-
wild-type soybean inhibitor
-
WBTI
-
zeamatin
-
-
-
Zn2+
-
18% inhibition at 5 mM
Zn2+
-
71% inhibition at 5 mM
[4-(6-chloro-naphthalene-2-sulphonyl)piperazin-1-yl]-(3,4,5,6-tetrahydro-[2H-1,4']bipyridinyl-4-yl)-methanone
-
-
[Abu(3, 11)]-sun flower trypsin inhibitor-1
-
-
-
[Arg5]-SFTI-1
-
a wild-type SFTI-1 analogue
[Lys5]-SFTI-1
-
a wild-type SFTI-1 analogue
[N,N'-bis(m-amidinosalicylidene)ethylenediaminato]copper(II)
-
-
-
[N,N'-bis(m-guanidinosalicylidene)ethylenediaminato]copper(II)
-
-
-
[Phe5]-SFTI-1
-
a wild-type SFTI-1 analogue
additional information
-
naturally occurring trypsin inhibitor SFTI-1 isolated from sunflower seeds and its analogues
-
additional information
-
4-guanidinebenzoate: no inhibition up to 0.2 mM
-
additional information
-
not inhibitory: EDTA, tosyl-L-Phe chloromethyl ketone
-
additional information
-
not inhibitory: Ca2+ up to 500 mM
-
additional information
-
not inhibitory: chymostatin
-
additional information
-
trypsin 4 is completely resistant to chymotryptic degradation
-
additional information
-
not inhibited by 1-(L-trans-epoxysuccinyl-leucylamino)-4-guanidinobutane, N-ethylmaleimide, iodoacetic acid, N-tosyl-L-phenyl-alanine-chloromethyl ketone, and pepstatin
-
additional information
-
mesotrypsin exhibits complete resistance against wild-type alpha1-antitrypsin
-
additional information
-
1-(L-trans-epoxysuccinyl-leucylamino)-4-guanidinobutane, N-ethylmaleimide, iodoacetic acid, chymotrypsin, pepstatin A, and EDTA have no inhibitory effect
-
additional information
-
notinhibited by pepstatin A
-
additional information
-
resistant to soybean trypsin inhibitor and aprotinin
-
additional information
-
trypsin is not inhibited by N-(4-aminobutyl)-L-Arg-L-Cys-L-Thr-L-Lys-L-Ser-L-Ile-L-Pro-L-Pro-L-Ile-L-Cys-L-Phe-L-Pro-L-Asp and N-benzylglycine-L-Arg-L-Cys-L-Thr-L-Lys-L-Ser-L-Ile-L-Pro-L-Pro-L-Ile-L-Cys-L-Phe-L-Pro-L-Asp
-
additional information
-
trypsin activity decreases with age, trypsin activity correlates inversely with cholesterol concentration and plasmin and elastase activity
-
additional information
-
aspartate (PepA) and cysteine (E64) protease inhibitors as well as the chymotrypsin inhibitor tosyl-L-phenylalanine chloromethylketone have no effect on enzymatic activity of isozyme TryIII
-
additional information
-
not inhibited by up to 0.05 mM SVIGCWTFSIPPRPCFVK-amide
-
additional information
-
trypsin is not inhibited by N-(4-aminobutyl)-L-Arg-L-Cys-L-Thr-L-Lys-L-Ser-L-Ile-L-Pro-L-Pro-L-Ile-L-Cys-L-Phe-L-Pro-L-Asp and N-benzylglycine-L-Arg-L-Cys-L-Thr-L-Lys-L-Ser-L-Ile-L-Pro-L-Pro-L-Ile-L-Cys-L-Phe-L-Pro-L-Asp, Gly-L-Arg-(2-amino butanoic acid)-L-Thr-Nlys-L-Ser-L-Ile-L-Pro-L-Pro-L-Ile-(2-amino butanoyl)-L-Phe-L-Pro-L-Asp, L-Lys-(2-amino butanoyl)-L-Thr-Nlys-L-Ser-L-Ile-L-Pro-L-Pro-L-Ile-(2-amino butanoyl)-L-Phe-L-Pro-L-Asp, and Nlys-(2-amino butanoyl)-L-Thr-Nlys-L-Ser-L-Ile-L-Pro-L-Pro-L-Ile-(2-amino butanoyl)-L-Phe-L-Pro-L-Asp
-
additional information
-
the crude and partially purified extract from Passiflora f. edulis flavicarpa leaves displays inhibitory effect towards trypsin, methyl jasmonate treatment of the leaves increases the inhibitory activity against trypsin
-
additional information
-
no inhibition by N-alpha-tosyl-L-Phe-chloromethyl ketone, E-64, and O-fenantroline
-
additional information
-
identification of trypsin-3 binding peptides, and molecular modeling of the binding of peptides to isozyme trypsin-3. The degradation rate of peptides by trypsin correlates to their inhibitory efficacy, the most stable peptides being the most effective inhibitors, overview
-
additional information
-
design and synthesis of Schiff base metal chelate inhibitors of trypsin, structural basis, overview. The binding mode of the guanidino groups of m-guanidinosalicylidene-L-alaninato(aqua)copper(II) hydrochloride and [N,N'-bis(m-guanidinosalicylidene)ethylenediaminato]copper(II) to Asp189 in the S1 pocket of trypsin is markedly different from of the amidino group of m-amidinosalicylidene-L-alaninato(aqua)copper(II) hydrochloride. The active site residues of trypsin play a crucial role in the binding affinity to the trypsin molecule.
-
additional information
-
no inhibition by EDTA, E-64, and pepstatin A
-
additional information
-
no or poor inhibtion by pepstatin A, 2-mercaptoethanol, Ca2+, K+, and Na+
-
additional information
-
no inhibition by pepstatin A, 2-mercaptoethanol and EDTA
-
additional information
-
no or poor inhibition by E-64, N-ethylmaleimide, iodoacetic acid, TPCK, pepstatin A, and EDTA
-
additional information
-
the enzyme activity is completely stable in presence of detergents, overview. No inhibition by EDTA, 2-nercaptoethanol, pepstatin A, Ca2+, Ba2+, Zn2+, Cu2+, Mg2+, Mn2+, K+, and Na+
-
additional information
-
no or poor inhibition by N-tosyl-L-phenylalanine chloromethylketone, EDTA, 2-mercaptoethanol, pepstatin A, iodoacetic acid, and E-64
-
additional information
-
no inhibition by E-64, N-ethylmaleimide, iodoacetic acid, pepstatin A, and Nalpha-4-tosyl-L-Phe-chloromethylketone
-
additional information
-
no or poor inhibition by pepstatin A, EDTA, 2-mercaptoethanol, and N-p-tosyl-L-phenylalanine chloromethyl ketone
-
additional information
-
determination of serum trypsin inhibitory capacity of plasma in psoriatic patients, which is increased compared to healthy persons
-
additional information
-
inhinitory potencies of the different legume trypsin inhibitors, overview
-
additional information
-
infection of Lutzomyia longipalpis with Leishmania mexicana reduces trypsin-like activity in the sand fly midgut, overview
-
additional information
-
no or poor inhibition of trypsin A and trypsin B by pepstatin at 0.001 mM, and EDTA and 1,10-phenathroline at 10 mM
-
additional information
-
no inhibition by DTNB and 2-mercaptoethanol
-
additional information
-
no inhibition by N-ethylmaleimide
-
additional information
-
effective inhibition of the catalytic activity by metal ions observed in trypsin R96H is caused by specific and reversible reorganization of the active site in the enzyme
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
caerulein
-
trypsinogen activation to trypsin occurs with 10 nM caerulein, trypsinogen is activated to reach a trypsin level of 6 nM following 10 nM caerulein induction for 1 h
dithiothreitol
-
addition of 2 mM dithiothreitol greatly enhances the tryptic activity
EDTA
-
increase in activation of pro-matrix metalloproteinase-2
enterokinase
-
trypsin is activated with 100 U/l enterokinase
-
enterokinase
-
trypsinogens (0.0002 mM) are incubated at room temperature with 6-200 ng enterokinase in 0.1 M Tris (pH 8), containing 5 mM CaCl2 and 0.01% bovine serum albumin for 10-60 min
-
enteropeptidase
-
trypsinogen is activated through the cleavage of a peptide Val-Asp-Asp-Asp-Asp-Lys-Ile located on the N-terminus of the protein, the heptapetide is cleaved autocatalytically by its physiological activator, enteropeptidase
-
methyl-alpha-D-galactoside
-
1.2fold enhancement of activity at 0.2 M
Na+
-
wild-type, no activation, engineering of wild-type into a Na+-activated enzyme by replacing residues in the 170, 186, and 220 loops to those of coagulation factor Xa
methyl-alpha-D-mannoside
-
1.4fold enhancement of activity at 0.2 M
additional information
-
increase in catalytic efficiency of the trypsin reaction with decreasing dielectric constant results from the stabilization of electrostatic energy for the formation of an oxyanion hole, and this stabilization is caused by the increase of electrostricted water around the charged tetrahedral transition state. The control of the solvent dielectric constant can stabilize the tetrahedral transition state, and this lowers the activation energy
-
additional information
-
activity is not enhanced in 0.2 M lactose
-
additional information
-
tryptase expression in human skin is significantly increased at 24 and 48 h postirradiation with ultraviolet, infrared, and heat treatment
-
additional information
-
pancreatic trypsin activity increases linearly with increasing dietary crude protein concentration
-
additional information
-
colonic trypsin activity increases during acute stress
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.0069
-
Abz-ERFK-EDDnp
-
pH 8.0, 25C
0.0005
-
Abz-FRFK-EDDnp
-
pH 8.0, 25C
0.0163
-
Abz-GRFK-EDDnp
-
pH 8.0, 25C
0.0068
-
Abz-HRFK-EDDnp
-
pH 8.0, 25C
0.0006
-
Abz-KRFK-EDDnp
-
pH 8.0, 25C
0.001
-
Abz-LRFK-EDDnp
-
pH 8.0, 25C
0.0074
-
Abz-NRFK-EDDnp
-
pH 8.0, 25C
0.0089
-
Abz-QRFK-EDDnp
-
pH 8.0, 25C
0.0081
-
Abz-RDFK-EDDnp
-
pH 8.0, 25C
0.0129
-
Abz-REFK-EDDnp
-
pH 8.0, 25C
0.0159
-
Abz-RGFK-EDDnp
-
pH 8.0, 25C
0.00185
-
Abz-RHFK-EDDnp
-
pH 8.0, 25C
0.0049
-
Abz-RNFK-EDDnp
-
pH 8.0, 25C
0.0031
-
Abz-RPFK-EDDnp
-
pH 8.0, 25C
0.0074
-
Abz-RQFK-EDDnp
-
pH 8.0, 25C
0.0017
-
Abz-RRFK-EDDnp
-
pH 8.0, 25C
0.001
-
Abz-RSFK-EDDnp
-
pH 8.0, 25C
0.00325
-
Abz-RTFK-EDDnp
-
pH 8.0, 25C
0.0183
-
Abz-RVFK-EDDnp
-
pH 8.0, 25C
0.0017
-
Abz-RYFK-EDDnp
-
pH 8.0, 25C
0.0029
-
Abz-VRFK-EDDnp
-
pH 8.0, 25C
0.0033
-
Abz-WRFK-EDDnp
-
pH 8.0, 25C
0.0056
-
Abz-YRFK-EDDnp
-
pH 8.0, 25C
0.242
-
acetyl-Ala-Val-(L-alpha-aminobutyryl)-Pro-Arg(NO2)-4-nitroanilide
-
-
0.015
-
acetyl-Ala-Val-(L-alpha-aminobutyryl)-Pro-Arg-4-nitroanilide
-
-
0.236
-
acetyl-Ala-Val-(L-alpha-aminobutyryl)-Pro-Har-4-nitroanilide
-
-
0.017
-
acetyl-Ala-Val-(L-alpha-aminobutyryl)-Pro-Lys-4-nitroanilide
-
-
0.361
-
acetyl-Ala-Val-(L-alpha-aminobutyryl)-Pro-Phe(p-CN)-4-nitroanilide
-
-
0.384
-
acetyl-Ala-Val-(L-alpha-aminobutyryl)-Pro-Phe(p-NH2)-4-nitroanilide
-
-
0.328
-
alpha-N-p-tosyl-L-arginine methyl ester
-
pH 8.5, 60C
0.033
-
benzoyl-Arg 4-nitroanilide
-
pH 8.0, 25C, isoenzyme aT-II
0.037
-
benzoyl-Arg 4-nitroanilide
-
pH 8.0, 25C, isoenzyme aT-I
0.0026
-
benzoyl-Arg ethyl ester
-
alpha-trypsin
0.014
-
benzoyl-Arg ethyl ester
-
pH 8.0, 25C, isoenzyme aT-II
0.031
-
benzoyl-Arg ethyl ester
-
pH 8.0, 25C, isoenzyme aT-I
0.09
-
benzoyl-Arg ethyl ester
-
gamma-trypsin
0.034
-
benzoyl-Arg-p-nitroanilide
-
at 25C
0.43
-
benzoyl-Arg-p-nitroanilide
-
at 25C
0.07744
-
benzoyl-DL-Arg-7-amido-4-methylcoumarin
-
in 10 mM Tris, 20 mM CaCl2, pH 7.4, at 37C
3
-
benzoyl-DL-Arg-p-nitroanilide
-
in 10 mM Tris, 20 mM CaCl2, pH 7.4, at 37C
0.02
-
benzoyl-L-arginine-p-nitroanilide
-
isozyme ST-1, at 5C; isozyme ST-2, at 5C; isozyme ST-3, at 15C; isozyme ST-3, at 25C; isozyme ST-3, at 5C
0.03
-
benzoyl-L-arginine-p-nitroanilide
-
isozyme ST-1, at 15C; isozyme ST-1, at 25C; isozyme ST-2, at 15C
0.04
-
benzoyl-L-arginine-p-nitroanilide
-
isozyme ST-2, at 25C; isozyme ST-3, at 35C
0.05
-
benzoyl-L-arginine-p-nitroanilide
-
isozyme ST-2, at 35C
0.07
-
benzoyl-L-arginine-p-nitroanilide
-
isozyme ST-1, at 35C
0.2
-
benzoyl-L-arginine-p-nitroanilide
-
at 5C
0.26
-
benzoyl-L-arginine-p-nitroanilide
-
at 25C
0.27
-
benzoyl-L-arginine-p-nitroanilide
-
at 15C
0.44
-
benzoyl-L-arginine-p-nitroanilide
-
at 35C
0.0141
-
benzyloxycarbonyl-Gly-Pro-Arg-4-methylcoumarin 7-amide
-
wild-type enzyme
0.0162
-
benzyloxycarbonyl-Gly-Pro-Arg-4-methylcoumarin 7-amide
-
mutant enzyme E151Q
0.0177
-
benzyloxycarbonyl-Gly-Pro-Arg-4-methylcoumarin 7-amide
-
mutant enzyme N143H
0.0182
-
benzyloxycarbonyl-Gly-Pro-Arg-4-methylcoumarin 7-amide
-
mutant enzyme E151H
0.017
-
benzyloxycarbonyl-Gly-Pro-Arg-p-nitroanilide
-
at 25C
0.00038
-
benzyloxycarbonyl-L-glutaminyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide
-
pH 8.0, 37C, trypsin B
-
0.00054
-
benzyloxycarbonyl-L-glutaminyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide
-
pH 8.0, 37C, trypsin A
-
0.00028
-
benzyloxycarbonyl-L-leucyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide
-
pH 8.0, 37C, trypsin A
-
0.00036
-
benzyloxycarbonyl-L-leucyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide
-
pH 8.0, 37C, trypsin B
-
0.00039
-
benzyloxycarbonyl-L-leucyl-L-lysyl-L-arginine-4-methylcoumaryl-7-amide
-
pH 8.0, 37C, trypsin A
-
0.00082
-
benzyloxycarbonyl-L-leucyl-L-lysyl-L-arginine-4-methylcoumaryl-7-amide
-
pH 8.0, 37C, trypsin B
-
0.0007
-
benzyloxycarbonyl-L-phenylalaninyl-L-seryl-L-arginine-4-methylcoumaryl-7-amide
-
pH 8.0, 37C, trypsin B
-
0.00112
-
benzyloxycarbonyl-L-phenylalaninyl-L-seryl-L-arginine-4-methylcoumaryl-7-amide
-
pH 8.0, 37C, trypsin A
-
0.0003
-
benzyloxycarbonyl-L-valyl-L-leucyl-L-lysine-4-methylcoumaryl-7-amide
-
pH 8.0, 37C, trypsin A
-
0.00053
-
benzyloxycarbonyl-L-valyl-L-leucyl-L-lysine-4-methylcoumaryl-7-amide
-
pH 8.0, 37C, trypsin B
-
0.00058
-
benzyloxycarbonyl-L-valyl-L-prolyl-L-arginine-4-methylcoumaryl-7-amide
-
pH 8.0, 37C, trypsin A
-
0.00059
-
benzyloxycarbonyl-L-valyl-L-prolyl-L-arginine-4-methylcoumaryl-7-amide
-
pH 8.0, 37C, trypsin B
-
0.08
-
benzyloxycarbonyl-Lys benzoyl ester
-
alpha-trypsin
-
22
-
benzyloxycarbonyl-Lys benzoyl ester
-
gamma-trypsin
-
0.0285
-
Benzyloxycarbonyl-Lys methyl ester
-
gamma-trypsin
0.125
-
Benzyloxycarbonyl-Lys methyl ester
-
alpha-trypsin
0.01
-
benzyloxycarbonyl-Lys p-nitrophenyl ester
-
alpha-trypsin
1.6
-
benzyloxycarbonyl-Lys p-nitrophenyl ester
-
gamma-trypsin
0.086
-
Benzyloxycarbonyl-Lys-thiobenzyl ester
-
wild-type enzyme
2.1
-
Benzyloxycarbonyl-Lys-thiobenzyl ester
-
mutant D189H
0.0061
-
benzyloxycarbonylglycyl-L-prolyl-L-arginine-4-methylcoumaryl-7-amide
-
wild-type enzyme, pH 8.0, 37C
-
0.012
-
benzyloxycarbonylglycyl-L-prolyl-L-arginine-4-methylcoumaryl-7-amide
-
mutant L71Y/Q72R, pH 8.0, 37C
-
0.0172
-
benzyloxycarbonylglycyl-L-prolyl-L-arginine-4-methylcoumaryl-7-amide
-
mutant L71Y, pH 8.0, 37C
-
0.0175
-
benzyloxycarbonylglycyl-L-prolyl-L-arginine-4-methylcoumaryl-7-amide
-
mutant Y71L, pH 8.0, 37C
-
0.0177
-
benzyloxycarbonylglycyl-L-prolyl-L-arginine-4-methylcoumaryl-7-amide
-
wild-type enzyme, pH 8.0, 37C
-
0.24
-
Bovine serum albumin
-
-
-
0.00399
-
enhanced green fluorescent protein-T1
-
in 10 mM Tris, 20 mM CaCl2, pH 7.4, at 37C
-
0.00421
-
enhanced green fluorescent protein-T1nb
-
in 10 mM Tris, 20 mM CaCl2, pH 7.4, at 37C
-
0.0089
-
factor Xa
-
wild-type, pH 8.0, 25C
-
0.0205
-
factor Xa
-
mutant Y217E/P222K/Y224K/P225Y, pH 8.0, 25C
-
0.0222
-
factor Xa
-
mutant G186K/G187Q/V188E/Y217E/P222K/Y224K/P225Y, pH 8.0, 25C
-
0.285
-
factor Xa
-
mutant Y172S/Y217E/P222K/Y224K/P225Y, pH 8.0, 25C
-
0.00015
-
FITC-casein
-
pH 7.0, 37C
-
0.00016
-
FITC-casein
-
pH 8.6, 37C
-
0.000209
-
FITC-casein
-
pH 7.5, 37C
-
0.000215
-
FITC-casein
-
pH 8.1, 37C
-
0.00086
-
FITC-casein
-
pH 7.8, 30C
-
0.00298
-
FITC-casein
-
pH 7.8, 58C
-
0.00333
-
FITC-casein
-
pH 7.8, 37C
-
0.00354
-
FITC-casein
-
pH 7.8, 48C
-
0.485
-
Glu-Gly-Arg-4-nitroanilide
-
pH 7.4, 25C
0.03
-
L-Phe-L-Val-L-Ile-N-(3-carbamoyl-4-nitrophenyl)-L-Arg-amide
-
-
0.096
-
L-Phe-L-Val-L-Ile-N-(3-carbamoyl-4-nitrophenyl)-L-Lys-amide
-
-
0.044
-
L-Phe-L-Val-L-Pro-N-(3-carbamoyl-4-nitrophenyl)-L-Arg-amide
-
-
0.24
-
L-Phe-L-Val-L-Pro-N-(3-carbamoyl-4-nitrophenyl)-L-Lys-amide
-
-
0.027
-
MeOSuc-Ala-Ala-Ala-Arg 4-nitroanilide
-
pH 8.0, 25C
0.013
-
MeOSuc-Ala-Ala-Arg 4-nitroanilide
-
pH 8.0, 25C
32
-
N-alpha-benzoyl-L-Arg ethyl ester
-
enzyme with O-carboxymethyl-poly-beta-cyclodextrin attached to surface, pH 8.0, 25C
35.5
-
N-alpha-benzoyl-L-Arg ethyl ester
-
wild-type, pH 8.0, 25C
0.55
-
N-alpha-benzoyl-L-Arg-p-nitroanilide
Q54137
recombinant enzyme, in 100 mM Tris-HCl, pH 8.0 at 25C
7.06
-
N-alpha-benzoyl-L-Arg-p-nitroanilide
-
recombinant enzyme, in 100 mM Tris-HCl, pH 8.0 at 25C
2.7
-
N-alpha-benzoyl-L-arginine 4-nitroanilide
-
pH 8.3, 25C, recombinant enzyme from Zea may
3.2
-
N-alpha-benzoyl-L-arginine 4-nitroanilide
-
pH 8.3, 25C, native bovine trypsin
1.26
-
N-alpha-benzoyl-L-arginine ethyl ester
-
in 20 mM TBS (pH 7.6) for 10 min at 25C, in the absence of sugars or glycoproteins
2.81
-
N-alpha-benzoyl-L-arginine ethyl ester
-
in 20 mM TBS (pH 7.6) for 10 min at 25C, in the presence of 0.2 M methyl-alpha-D-galactoside
3.2
-
N-alpha-benzoyl-L-arginine ethyl ester
-
in 20 mM TBS (pH 7.6) for 10 min at 25C, in the presence of 0.2 M methyl-alpha-D-mannoside
0.06
-
N-alpha-benzoyl-L-arginine-p-nitroanilide
-
isozyme TLE II, at 37C, pH 8.0
0.09
-
N-alpha-benzoyl-L-arginine-p-nitroanilide
-
isozyme TLE I, at 37C, pH 8.0
0.68
-
N-alpha-benzoyl-L-Lys-4-nitroanilide
Q54137
recombinant enzyme, in 100 mM Tris-HCl, pH 8.0 at 25C
5.7
-
N-alpha-benzoyl-L-Lys-4-nitroanilide
-
recombinant enzyme, in 100 mM Tris-HCl, pH 8.0 at 25C
0.2
-
N-alpha-p-tosyl-L-arginine methyl ester
-
trypsin B, at pH 8.0 and 30C
0.33
-
N-alpha-p-tosyl-L-arginine methyl ester
-
trypsin A, at pH 8.0 and 30C
0.158
-
N-benzoyl-DL-Arg-4-nitroanilide
-
-
0.05
-
N-benzoyl-DL-arginine 4-nitroanilide
-
pH 8.0, 25C
0.064
-
N-benzoyl-DL-arginine 4-nitroanilide
-
pH 8.0, 25C
0.13
-
N-benzoyl-DL-arginine 4-nitroanilide
-
pH 9.0, 25C
0.387
-
N-benzoyl-DL-arginine 4-nitroanilide
-
pH 8.5, 25C
0.507
-
N-benzoyl-DL-arginine 4-nitroanilide
-
pH 8.5, 60C
0.0077
-
N-benzoyl-L-Arg ethyl ester
-
-
0.88
-
N-benzoyl-L-arginine-p-nitroanilide
-
in 67 mM sodium phosphate buffer, pH 7.6, at 25C
0.007
-
N-benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide
-
pH 8.0, 25C
0.009
-
N-benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide
-
pH 9.0, 25C
0.015
-
N-benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide
-
pH 7.1, 25C
0.034
-
N-benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide
-
pH 6.0, 25C
0.0135
-
N-carbobenzyloxy-Gly-Pro-Arg-p-nitroanilide
-
mutant enzyme R193G, in 50 mM Tricine, 10 mM CaCl2, pH 8.0 at 20C
0.0198
-
N-carbobenzyloxy-Gly-Pro-Arg-p-nitroanilide
-
mutant enzyme R193A, in 50 mM Tricine, 10 mM CaCl2, pH 8.0 at 20C
0.0213
-
N-carbobenzyloxy-Gly-Pro-Arg-p-nitroanilide
-
mutant enzyme R193Y, in 50 mM Tricine, 10 mM CaCl2, pH 8.0 at 20C
0.0252
-
N-carbobenzyloxy-Gly-Pro-Arg-p-nitroanilide
-
wild type enzyme, in 50 mM Tricine, 10 mM CaCl2, pH 8.0 at 20C
0.0274
-
N-carbobenzyloxy-Gly-Pro-Arg-p-nitroanilide
-
mutant enzyme R193F, in 50 mM Tricine, 10 mM CaCl2, pH 8.0 at 20C
0.15
-
Nalpha-4-tosyl-L-arginine methyl ester
-
pH 8.5, 45C
0.156
-
Nalpha-4-tosyl-L-arginine methyl ester
-
pH 8.5, 25C
0.077
-
Nalpha-benzoyl-DL-Arg-p-nitroanilide
-
-
0.197
-
Nalpha-benzoyl-DL-Arg-p-nitroanilide
-
cationic trypsin 1
0.244
-
Nalpha-benzoyl-DL-Arg-p-nitroanilide
-
cationic trypsin 2
0.619
-
Nalpha-benzoyl-DL-Arg-p-nitroanilide
-
anionic trypsin
0.99
-
Nalpha-benzoyl-DL-Arg-p-nitroanilide
-
anionic enzyme
1.12
-
Nalpha-benzoyl-DL-Arg-p-nitroanilide
-
anionic enzyme
0.068
-
Nalpha-benzoyl-DL-arginine 4-nitroanilide
-
pH 10.5, 25C
0.083
-
Nalpha-benzoyl-DL-arginine-4-nitroanilide
-
isozyme trypsin B, pH 9.0, 25C
0.1
-
Nalpha-benzoyl-DL-arginine-4-nitroanilide
-
isozyme trypsin C, pH 9.0, 25C
0.125
-
Nalpha-benzoyl-DL-arginine-4-nitroanilide
-
isozyme trypsin A, pH 9.0, 25C
0.051
-
Nalpha-benzoyl-L-Arg-4-nitroanilide
-
pH 8.0, 25C
0.0012
-
Nalpha-benzyloxycarbonyl-Lys-p-nitrophenyl ester
-
without addition of methanol
-
0.0038
-
Nalpha-benzyloxycarbonyl-Lys-p-nitrophenyl ester
-
in presence of 10% methanol
-
0.0096
-
Nalpha-benzyloxycarbonyl-Lys-p-nitrophenyl ester
-
in presence of 20% methanol
-
0.0176
-
Nalpha-benzyloxycarbonyl-Lys-p-nitrophenyl ester
-
in presence of 30% methanol
-
0.168
-
Nalpha-benzyloxycarbonyl-Lys-p-nitrophenyl ester
-
in presence of 50% methanol
-
0.25
-
Nalpha-p-tosyl-L-Arg methyl ester
-
pH 8.0, 30C
0.0018
-
o-aminobenzoyl-AAGRGAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.0013
-
o-aminobenzoyl-AAGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.005
-
o-aminobenzoyl-AAGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.045
-
o-aminobenzoyl-AAGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.0073
-
o-aminobenzoyl-ADGRGAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.0057
-
o-aminobenzoyl-ADGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.0037
-
o-aminobenzoyl-AEGRGAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.0033
-
o-aminobenzoyl-AEGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.01
-
o-aminobenzoyl-AEGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.017
-
o-aminobenzoyl-AEGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.0029
-
o-aminobenzoyl-AFGRGAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.002
-
o-aminobenzoyl-AFGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.0071
-
o-aminobenzoyl-AFGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.008
-
o-aminobenzoyl-AGARGAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.0025
-
o-aminobenzoyl-AGARGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.027
-
o-aminobenzoyl-AGARGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.044
-
o-aminobenzoyl-AGARGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.0038
-
o-aminobenzoyl-AGDRGAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.016
-
o-aminobenzoyl-AGDRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.019
-
o-aminobenzoyl-AGDRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.036
-
o-aminobenzoyl-AGDRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.0091
-
o-aminobenzoyl-AGERGAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.0053
-
o-aminobenzoyl-AGERGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.022
-
o-aminobenzoyl-AGERGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.023
-
o-aminobenzoyl-AGERGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.0018
-
o-aminobenzoyl-AGFRGAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.0031
-
o-aminobenzoyl-AGFRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.009
-
o-aminobenzoyl-AGFRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.019
-
o-aminobenzoyl-AGFRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.01
-
o-aminobenzoyl-AGGKGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.011
-
o-aminobenzoyl-AGGKGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.016
-
o-aminobenzoyl-AGGKGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.0233
-
o-aminobenzoyl-AGGKGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.0037
-
o-aminobenzoyl-AGGRAAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.008
-
o-aminobenzoyl-AGGRDAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.018
-
o-aminobenzoyl-AGGREAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.0041
-
o-aminobenzoyl-AGGRFAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.0035
-
o-aminobenzoyl-AGGRGAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.0031
-
o-aminobenzoyl-AGGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.006
-
o-aminobenzoyl-AGGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.0074
-
o-aminobenzoyl-AGGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.015
-
o-aminobenzoyl-AGGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.015
-
o-aminobenzoyl-AGGRGDGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.013
-
o-aminobenzoyl-AGGRGEGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.009
-
o-aminobenzoyl-AGGRGFGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.0024
-
o-aminobenzoyl-AGGRGGGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.0032
-
o-aminobenzoyl-AGGRGHGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.003
-
o-aminobenzoyl-AGGRGIGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.0009
-
o-aminobenzoyl-AGGRGKGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.002
-
o-aminobenzoyl-AGGRGLGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.0024
-
o-aminobenzoyl-AGGRGMGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.0037
-
o-aminobenzoyl-AGGRGNGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.0028
-
o-aminobenzoyl-AGGRGQGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.0029
-
o-aminobenzoyl-AGGRGSGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.007
-
o-aminobenzoyl-AGGRGTGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.0027
-
o-aminobenzoyl-AGGRGVGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.006
-
o-aminobenzoyl-AGGRGYGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.0098
-
o-aminobenzoyl-AGGRHAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.0059
-
o-aminobenzoyl-AGGRIAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.002
-
o-aminobenzoyl-AGGRKAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.0036
-
o-aminobenzoyl-AGGRLAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.0025
-
o-aminobenzoyl-AGGRMAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.0062
-
o-aminobenzoyl-AGGRNAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.0039
-
o-aminobenzoyl-AGGRQAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.0085
-
o-aminobenzoyl-AGGRSAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.0043
-
o-aminobenzoyl-AGGRTAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.004
-
o-aminobenzoyl-AGGRVAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.004
-
o-aminobenzoyl-AGGRYAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.0005
-
o-aminobenzoyl-AGHRGAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.0006
-
o-aminobenzoyl-AGHRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.0083
-
o-aminobenzoyl-AGHRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.017
-
o-aminobenzoyl-AGHRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.04
-
o-aminobenzoyl-AGIRGAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.0031
-
o-aminobenzoyl-AGIRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.015
-
o-aminobenzoyl-AGIRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.029
-
o-aminobenzoyl-AGIRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.0018
-
o-aminobenzoyl-AGKRGAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.0053
-
o-aminobenzoyl-AGKRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.009
-
o-aminobenzoyl-AGKRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.018
-
o-aminobenzoyl-AGKRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.0017
-
o-aminobenzoyl-AGLRGAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.0013
-
o-aminobenzoyl-AGLRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.012
-
o-aminobenzoyl-AGLRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.0024
-
o-aminobenzoyl-AGMRGAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.0012
-
o-aminobenzoyl-AGMRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.015
-
o-aminobenzoyl-AGMRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.017
-
o-aminobenzoyl-AGMRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.0013
-
o-aminobenzoyl-AGNRGAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.0004
-
o-aminobenzoyl-AGNRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.017
-
o-aminobenzoyl-AGNRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.024
-
o-aminobenzoyl-AGNRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.011
-
o-aminobenzoyl-AGQRGAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.0033
-
o-aminobenzoyl-AGQRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.015
-
o-aminobenzoyl-AGQRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.025
-
o-aminobenzoyl-AGQRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.0051
-
o-aminobenzoyl-AGSRGAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.0011
-
o-aminobenzoyl-AGSRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.023
-
o-aminobenzoyl-AGSRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.027
-
o-aminobenzoyl-AGSRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.01
-
o-aminobenzoyl-AGTRGAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.0017
-
o-aminobenzoyl-AGTRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.0163
-
o-aminobenzoyl-AGTRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.0025
-
o-aminobenzoyl-AGVRGAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.0014
-
o-aminobenzoyl-AGVRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.018
-
o-aminobenzoyl-AGVRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.019
-
o-aminobenzoyl-AGVRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.0018
-
o-aminobenzoyl-AGYRGAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.0023
-
o-aminobenzoyl-AGYRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.011
-
o-aminobenzoyl-AGYRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.013
-
o-aminobenzoyl-AGYRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.0022
-
o-aminobenzoyl-AHGRGAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.0013
-
o-aminobenzoyl-AHGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.0017
-
o-aminobenzoyl-AIGRGAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.0015
-
o-aminobenzoyl-AIGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.0032
-
o-aminobenzoyl-AIGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.024
-
o-aminobenzoyl-AIGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.0012
-
o-aminobenzoyl-AKGRGAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.001
-
o-aminobenzoyl-AKGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.0079
-
o-aminobenzoyl-AKGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.0025
-
o-aminobenzoyl-ALGRGAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.0013
-
o-aminobenzoyl-ALGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.008
-
o-aminobenzoyl-ALGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.016
-
o-aminobenzoyl-ALGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.0018
-
o-aminobenzoyl-AMGRGAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.0008
-
o-aminobenzoyl-AMGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.014
-
o-aminobenzoyl-AMGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.016
-
o-aminobenzoyl-AMGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.0024
-
o-aminobenzoyl-ANGRGAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.0018
-
o-aminobenzoyl-ANGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.0064
-
o-aminobenzoyl-ANGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.0031
-
o-aminobenzoyl-AQGRGAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.0016
-
o-aminobenzoyl-AQGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.01
-
o-aminobenzoyl-AQGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.013
-
o-aminobenzoyl-AQGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.0024
-
o-aminobenzoyl-ASGRGAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.0017
-
o-aminobenzoyl-ASGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.019
-
o-aminobenzoyl-ASGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.0034
-
o-aminobenzoyl-ATGRGAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.0041
-
o-aminobenzoyl-ATGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.0017
-
o-aminobenzoyl-AVGRGAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.0015
-
o-aminobenzoyl-AVGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.013
-
o-aminobenzoyl-AVGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.037
-
o-aminobenzoyl-AVGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.0024
-
o-aminobenzoyl-AYGRGAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.0013
-
o-aminobenzoyl-AYGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.015
-
o-aminobenzoyl-AYGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.0387
-
p-amidinophenyl acetate
-
-
-
21
-
p-nitrophenyl acetate
-
-
0.029
-
p-tosyl-L-Arg methyl ester
-
-
0.023
-
Pefachrome
-
pH 8.0, wild-type enzyme
0.0342
-
Pefachrome
-
pH 8.0, mutant enzyme E97N/Y99L
0.0443
-
Pefachrome
-
pH 8.0, mutant enzyme S190A
0.0844
-
Pefachrome
-
pH 8.0, mutant enzyme E97N/Y99L/S190A
0.013
-
Pefachrome tPA
-
native enzyme
0.0229
-
Pefachrome tPA
-
variant X99rT containing the 99-loop (Glu97,Tyr99) of factor Xa
0.0472
-
Pefachrome tPA
-
variant X(99/175/190)rT containing the 99-loop the 175-loop (Ser172,Ser173, Phe174, Ile175) and Ala190 of factor Xa
0.00167
-
succinyl-Ala-Ala-Pro-Arg-7-amido-4-methylcoumarin
-
pH 8.0, 37C, mutant enzyme D189S/DELTA223
0.07
-
succinyl-Ala-Ala-Pro-Arg-7-amido-4-methylcoumarin
-
pH 8.0, 37C, wild-type enzyme
0.033
-
succinyl-Ala-Ala-Pro-Arg-p-nitroanilide
-
wild-type enzyme, pH 8
0.057
-
succinyl-Ala-Ala-Pro-Arg-p-nitroanilide
-
wild-type enzyme, pH 9
0.087
-
succinyl-Ala-Ala-Pro-Arg-p-nitroanilide
-
wild-type enzyme, pH 7
0.099
-
succinyl-Ala-Ala-Pro-Arg-p-nitroanilide
-
wild-type enzyme, pH 10
0.00217
-
succinyl-Ala-Ala-Pro-Leu-7-amido-4-methylcoumarin
-
pH 8.0, 37C, mutant enzyme D189S/DELTA223
0.31
-
succinyl-Ala-Ala-Pro-Leu-7-amido-4-methylcoumarin
-
pH 8.0, 37C, wild-type enzyme
0.004
-
succinyl-Ala-Ala-Pro-Lys-7-amido-4-methylcoumarin
-
pH 8.0, 37C, mutant enzyme D189S/DELTA223
0.1
-
succinyl-Ala-Ala-Pro-Lys-7-amido-4-methylcoumarin
-
pH 8.0, 37C, wild-type enzyme
0.101
-
succinyl-Ala-Ala-Pro-Lys-p-nitroanilide
-
wild-type enzyme, pH 9
0.105
-
succinyl-Ala-Ala-Pro-Lys-p-nitroanilide
-
wild-type enzyme, pH 8
0.184
-
succinyl-Ala-Ala-Pro-Lys-p-nitroanilide
-
wild-type enzyme, pH 10
0.839
-
succinyl-Ala-Ala-Pro-Lys-p-nitroanilide
-
wild-type enzyme, pH 7
0.004
-
succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin
-
pH 8.0, 37C, mutant enzyme D189S/DELTA223
0.78
-
succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin
-
pH 8.0, 37C, wild-type enzyme
0.000167
-
succinyl-Ala-Ala-Pro-Trp-7-amido-4-methylcoumarin
-
pH 8.0, 37C, mutant enzyme D189S/DELTA223
0.08
-
succinyl-Ala-Ala-Pro-Tyr-7-amido-4-methylcoumarin
-
pH 8.0, 37C, mutant enzyme D189S/DELTA223
0.2
-
succinyl-Ala-Ala-Pro-Tyr-7-amido-4-methylcoumarin
-
pH 8.0, 37C, wild-type enzyme
0.00927
-
tert-butoxycarbonyl-L-Gln-L-Ala-L-Arg-7-amido-4-methylcoumarin
-
in 10 mM Tris, 20 mM CaCl2, pH 7.4, at 37C
0.0305
-
tert-butyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin
-
pH 8.0, 30C
0.01
-
Tos-L-Arg-OMe
-
at 15C; at 25C; at 5C
0.02
-
Tos-L-Arg-OMe
-
at 35C
0.02
-
Tos-L-Arg-OMe
-
isozyme ST-1, at 5C; isozyme ST-2, at 15C; isozyme ST-2, at 25C; isozyme ST-2, at 5C; isozyme ST-3, at 25C; isozyme ST-3, at 5C
0.03
-
Tos-L-Arg-OMe
-
isozyme ST-1, at 15C; isozyme ST-2, at 35C; isozyme ST-3, at 15C
0.04
-
Tos-L-Arg-OMe
-
isozyme ST-1, at 25C; isozyme ST-3, at 35C
0.05
-
Tos-L-Arg-OMe
-
isozyme ST-1, at 35C
0.017
-
tosyl-Arg methyl ester
-
alpha-trypsin
0.06
-
tosyl-Arg methyl ester
-
gamma-trypsin
0.12
-
tosyl-Arg methyl ester
Cambarus affinis
-
free enzyme
1.53
-
tosyl-Arg methyl ester
Cambarus affinis
-
enzyme immobilized on Sepharose 4B-KT
2.5
-
tosyl-Arg methyl ester
Cambarus affinis
-
enzyme immobilized on porous glass loaded with isothiocyanate
6.2
-
tosyl-Arg methyl ester
Cambarus affinis
-
enzyme immobilized on Sepharose 4B-KT
0.00017
-
tosyl-Gly-Pro-Arg-4-nitroanilide
-
pH 8.3
0.000896
-
tosyl-Gly-Pro-Arg-4-nitroanilide
salmon
-
pH 8.3
0.002
-
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin
-
pH 7.6, recombinant enzyme
0.0023
-
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin
-
pH 7.6, wild-type enzyme
0.0032
-
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin
-
pH 7.6, recombinant enzyme
0.0036
-
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin
-
pH 7.6, wild-type enzyme
0.0044
-
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin
-
pH 7.6, mutant enzyme T190A
0.0061
-
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin
-
pH 7.6, mutant enzyme T190S
0.0101
-
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin
-
pH 7.6, mutant enzyme T190S
0.0129
-
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin
-
pH 7.6, mutant enzyme T190A
0.067
-
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin
-
pH 7.6, mutant enzyme T190P
0.166
-
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin
-
pH 7.6, mutant enzyme T190P
0.224
-
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin
-
pH 7.6, mutant enzyme T190V
0.231
-
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin
-
pH 7.6, mutant enzyme T190V
0.0141
-
Z-Gly-Pro-Arg-p-nitroanilide
-
mutant enzyme R193G/G142A, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20C
0.0203
-
Z-Gly-Pro-Arg-p-nitroanilide
-
mutant enzyme R193G, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20C
0.0266
-
Z-Gly-Pro-Arg-p-nitroanilide
-
mutant enzyme R193A, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20C
0.0298
-
Z-Gly-Pro-Arg-p-nitroanilide
-
wild type enzyme, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20C
0.0788
-
Z-Gly-Pro-Arg-p-nitroanilide
-
mutant enzyme R193G/G19A, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20C
0.346
-
Z-Gly-Pro-Arg-p-nitroanilide
-
mutant enzyme R193G/G184A, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20C
0.018
-
MeOSuc-Ala-Arg 4-nitroanilide
-
pH 8.0, 25C
additional information
-
additional information
-
kinetics
-
additional information
-
additional information
-
peptide synthesis using 4-amidino- and 4-guanidinophenyl esters of Nalpha-tert-butyloxycarbonyl-amino acid as acyl donor components with two types of acyl acceptors, overview
-
additional information
-
additional information
-
-
-
additional information
-
additional information
-
the mutations C191A and C220A decrease the activity of trypsin by 20-200fold as measured by the ratio of turnover number to Km-value for the hydrolysis of amide substrates, ester hydrolysis is decreased by less than 10fold
-
additional information
-
additional information
-
influence of pH-value on Km-value of wild-type and mutant enzymes
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
17.3
-
Abz-ERFK-EDDnp
-
pH 8.0, 25C
8.87
-
Abz-FRFK-EDDnp
-
pH 8.0, 25C
31.94
-
Abz-GRFK-EDDnp
-
pH 8.0, 25C
21.07
-
Abz-HRFK-EDDnp
-
pH 8.0, 25C
53.23
-
Abz-KRFK-EDDnp
-
pH 8.0, 25C
53.23
-
Abz-LRFK-EDDnp
-
pH 8.0, 25C
30.61
-
Abz-NRFK-EDDnp
-
pH 8.0, 25C
12.64
-
Abz-QRFK-EDDnp
-
pH 8.0, 25C
2.33
-
Abz-RDFK-EDDnp
-
pH 8.0, 25C
7.49
-
Abz-REFK-EDDnp
-
pH 8.0, 25C
9.15
-
Abz-RGFK-EDDnp
-
pH 8.0, 25C
8.32
-
Abz-RHFK-EDDnp
-
pH 8.0, 25C
16.63
-
Abz-RNFK-EDDnp
-
pH 8.0, 25C
0.1
-
Abz-RPFK-EDDnp
-
pH 8.0, 25C
13.31
-
Abz-RQFK-EDDnp
-
pH 8.0, 25C
26.61
-
Abz-RRFK-EDDnp
-
pH 8.0, 25C
16.63
-
Abz-RSFK-EDDnp
-
pH 8.0, 25C
18.3
-
Abz-RTFK-EDDnp
-
pH 8.0, 25C
4.59
-
Abz-RVFK-EDDnp
-
pH 8.0, 25C
10.65
-
Abz-RYFK-EDDnp
-
pH 8.0, 25C
46.57
-
Abz-VRFK-EDDnp
-
pH 8.0, 25C
6.32
-
Abz-WRFK-EDDnp
-
pH 8.0, 25C
27.72
-
Abz-YRFK-EDDnp
-
pH 8.0, 25C
1.4
-
acetyl-Ala-Val-(L-alpha-aminobutyryl)-Pro-Arg(NO2)-4-nitroanilide
-
-
23
-
acetyl-Ala-Val-(L-alpha-aminobutyryl)-Pro-Arg-4-nitroanilide
-
-
19
-
acetyl-Ala-Val-(L-alpha-aminobutyryl)-Pro-Lys-4-nitroanilide
-
-
3.2
-
acetyl-Ala-Val-(L-alpha-aminobutyryl)-Pro-N-omega-hydroxy-L-arginine-4-nitroanilide
-
-
0.7
-
acetyl-Ala-Val-(L-alpha-aminobutyryl)-Pro-Phe(p-CN)-4-nitroanilide
-
-
5.1
-
acetyl-Ala-Val-(L-alpha-aminobutyryl)-Pro-Phe(p-NH2)-4-nitroanilide
-
-
112
-
alpha-N-p-tosyl-L-arginine methyl ester
-
pH 8.5, 60C
1.94
-
benzoyl-Arg 4-nitroanilide
-
pH 8.0, 25C, isoenzyme aT-I
2.94
-
benzoyl-Arg 4-nitroanilide
-
pH 8.0, 25C, isoenzyme aT-I
3.7
-
benzoyl-Arg 4-nitroanilide
-
pH 8.0, 25C, isoenzyme aT-II
0.24
-
benzoyl-Arg ethyl ester
-
gamma-trypsin
0.3
-
benzoyl-Arg ethyl ester
-
pH 8.0, 25C, isoenzyme aT-II
26.6
-
benzoyl-Arg ethyl ester
-
alpha-trypsin
42.9
-
benzoyl-Arg ethyl ester
-
pH 8.0, 25C, isoenzyme aT-I
1.13
-
benzoyl-Arg-p-nitroanilide
-
at 25C
3.44
-
benzoyl-Arg-p-nitroanilide
-
at 25C
0.25
-
benzoyl-DL-Arg-7-amido-4-methylcoumarin
-
in 10 mM Tris, 20 mM CaCl2, pH 7.4, at 37C
1.33
-
benzoyl-DL-Arg-p-nitroanilide
-
in 10 mM Tris, 20 mM CaCl2, pH 7.4, at 37C
0.3
-
benzoyl-L-arginine-p-nitroanilide
-
at 5C
0.64
-
benzoyl-L-arginine-p-nitroanilide
-
isozyme ST-3, at 5C
0.76
-
benzoyl-L-arginine-p-nitroanilide
-
at 15C
0.83
-
benzoyl-L-arginine-p-nitroanilide
-
isozyme ST-2, at 5C
0.94
-
benzoyl-L-arginine-p-nitroanilide
-
at 25C
1.06
-
benzoyl-L-arginine-p-nitroanilide
-
isozyme ST-1, at 5C
1.27
-
benzoyl-L-arginine-p-nitroanilide
-
isozyme ST-3, at 15C
2
-
benzoyl-L-arginine-p-nitroanilide
-
isozyme ST-3, at 25C
2.43
-
benzoyl-L-arginine-p-nitroanilide
-
at 35C
2.72
-
benzoyl-L-arginine-p-nitroanilide
-
isozyme ST-2, at 15C
2.74
-
benzoyl-L-arginine-p-nitroanilide
-
isozyme ST-1, at 15C
3
6
benzoyl-L-arginine-p-nitroanilide
-
isozyme ST-1, at 15C
3.13
-
benzoyl-L-arginine-p-nitroanilide
-
isozyme ST-1, at 25C
3.86
-
benzoyl-L-arginine-p-nitroanilide
-
isozyme ST-3, at 35C
4.06
-
benzoyl-L-arginine-p-nitroanilide
-
isozyme ST-1, at 25C
5.54
-
benzoyl-L-arginine-p-nitroanilide
-
isozyme ST-2, at 25C
6.74
-
benzoyl-L-arginine-p-nitroanilide
-
isozyme ST-2, at 35C
7.7
-
benzoyl-L-arginine-p-nitroanilide
-
isozyme ST-1, at 35C
44.5
-
benzyloxycarbonyl-Gly-Pro-Arg-4-methylcoumarin 7-amide
-
mutant enzyme N143H
49.3
-
benzyloxycarbonyl-Gly-Pro-Arg-4-methylcoumarin 7-amide
-
mutant enzyme E151Q
58.3
-
benzyloxycarbonyl-Gly-Pro-Arg-4-methylcoumarin 7-amide
-
wild-type enzyme
69.3
-
benzyloxycarbonyl-Gly-Pro-Arg-4-methylcoumarin 7-amide
-
mutant enzyme E151H
0.01
-
benzyloxycarbonyl-Gly-Pro-Arg-p-nitroanilide
-
at 25C
172.8
-
benzyloxycarbonyl-Gly-Pro-Arg-p-nitroanilide
-
at 25C
41.14
-
benzyloxycarbonyl-L-glutaminyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide
-
pH 8.0, 37C, trypsin B
-
42.99
-
benzyloxycarbonyl-L-glutaminyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide
-
pH 8.0, 37C, trypsin A
-
4.65
-
benzyloxycarbonyl-L-leucyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide
-
pH 8.0, 37C, trypsin A
-
14.85
-
benzyloxycarbonyl-L-leucyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide
-
pH 8.0, 37C, trypsin B
-
5.42
-
benzyloxycarbonyl-L-leucyl-L-lysyl-L-arginine-4-methylcoumaryl-7-amide
-
pH 8.0, 37C, trypsin A
-
12.19
-
benzyloxycarbonyl-L-leucyl-L-lysyl-L-arginine-4-methylcoumaryl-7-amide
-
pH 8.0, 37C, trypsin B
-
67.79
-
benzyloxycarbonyl-L-phenylalaninyl-L-seryl-L-arginine-4-methylcoumaryl-7-amide
-
pH 8.0, 37C, trypsin B
-
72.08
-
benzyloxycarbonyl-L-phenylalaninyl-L-seryl-L-arginine-4-methylcoumaryl-7-amide
-
pH 8.0, 37C, trypsin A
-
3.2
-
benzyloxycarbonyl-L-valyl-L-leucyl-L-lysine-4-methylcoumaryl-7-amide
-
pH 8.0, 37C, trypsin A
-
8.07
-
benzyloxycarbonyl-L-valyl-L-leucyl-L-lysine-4-methylcoumaryl-7-amide
-
pH 8.0, 37C, trypsin B
-
5.51
-
benzyloxycarbonyl-L-valyl-L-prolyl-L-arginine-4-methylcoumaryl-7-amide
-
pH 8.0, 37C, trypsin A
-
25.75
-
benzyloxycarbonyl-L-valyl-L-prolyl-L-arginine-4-methylcoumaryl-7-amide
-
pH 8.0, 37C, trypsin B
-
8
-
benzyloxycarbonyl-Lys benzoyl ester
-
gamma-trypsin
-
84
-
benzyloxycarbonyl-Lys benzoyl ester
-
alpha-trypsin
-
0.71
-
Benzyloxycarbonyl-Lys methyl ester
-
gamma-trypsin
80
-
Benzyloxycarbonyl-Lys methyl ester
-
alpha-trypsin
23
-
benzyloxycarbonyl-Lys p-nitrophenyl ester
-
gamma-trypsin
82
-
benzyloxycarbonyl-Lys p-nitrophenyl ester
-
alpha-trypsin
12.3
-
Benzyloxycarbonyl-Lys-thiobenzyl ester
-
mutant D189H
112
-
benzyloxycarbonyl-Lys-thiobenzyl ester ester
-
wild-type enzyme
-
33
-
benzyloxycarbonylglycyl-L-prolyl-L-arginine-4-methylcoumaryl-7-amide
-
wild-type enzyme, pH 8.0, 37C
-
51.3
-
benzyloxycarbonylglycyl-L-prolyl-L-arginine-4-methylcoumaryl-7-amide
-
mutant L71Y, pH 8.0, 37C
-
57.3
-
benzyloxycarbonylglycyl-L-prolyl-L-arginine-4-methylcoumaryl-7-amide
-
mutant L71Y/Q72R, pH 8.0, 37C
-
93
-
benzyloxycarbonylglycyl-L-prolyl-L-arginine-4-methylcoumaryl-7-amide
-
mutant Y71L, pH 8.0, 37C
-
279.5
-
benzyloxycarbonylglycyl-L-prolyl-L-arginine-4-methylcoumaryl-7-amide
-
wild-type enzyme, pH 8.0, 37C
-
0.26
-
enhanced green fluorescent protein-T1
-
in 10 mM Tris, 20 mM CaCl2, pH 7.4, at 37C
-
0.85
-
enhanced green fluorescent protein-T1nb
-
in 10 mM Tris, 20 mM CaCl2, pH 7.4, at 37C
-
3
-
factor Xa
-
mutant Y172S/Y217E/P222K/Y224K/P225Y, pH 8.0, 25C
-
9.25
-
factor Xa
-
mutant G186K/G187Q/V188E/Y217E/P222K/Y224K/P225Y, pH 8.0, 25C
-
77.7
-
factor Xa
-
mutant Y172S/Y217E/P222K/Y224K/P225Y, pH 8.0, 25C
-
86.4
-
factor Xa
-
mutant Y217E/P222K/Y224K/P225Y, pH 8.0, 25C
-
124
-
factor Xa
-
wild-type, pH 8.0, 25C
-
0.32
-
FITC-casein
-
pH 7.0, 37C
-
0.35
-
FITC-casein
-
pH 7.8, 30C
-
0.4
-
FITC-casein
-
pH 7.5, 37C
-
0.43
-
FITC-casein
-
pH 8.6, 37C
-
0.44
-
FITC-casein
-
pH 7.8, 37C; pH 8.1, 37C
-
0.48
-
FITC-casein
-
pH 7.8, 48C; pH 7.8, 58C
-
3.2
-
L-Phe-L-Val-L-Ile-(3-carbamoyl-4-nitrophenyl)-L-Arg-amide
-
-
4.4
-
L-Phe-L-Val-L-Ile-N-(3-carbamoyl-4-nitrophenyl)-L-Arg-amide
-
-
27.4
-
L-Phe-L-Val-L-Pro-N-(3-carbamoyl-4-nitrophenyl)-L-Arg-amide
-
-
4
-
L-Phe-L-Val-L-Pro-N-(3-carbamoyl-4-nitrophenyl)-L-Lys-amide
-
-
30.5
-
MeOSuc-Ala-Ala-Ala-Arg 4-nitroanilide
-
pH 8.0, 25C
33.6
-
MeOSuc-Ala-Ala-Arg 4-nitroanilide
-
pH 8.0, 25C
10.5
-
N-alpha-benzoyl-L-Arg ethyl ester
-
enzyme with O-carboxymethyl-poly-beta-cyclodextrin attached to surface, pH 8.0, 25C
12.3
-
N-alpha-benzoyl-L-Arg ethyl ester
-
wild-type, pH 8.0, 25C
2.46
-
N-alpha-benzoyl-L-Arg-p-nitroanilide
-
recombinant enzyme, in 100 mM Tris-HCl, pH 8.0 at 25C
5.73
-
N-alpha-benzoyl-L-Arg-p-nitroanilide
Q54137
recombinant enzyme, in 100 mM Tris-HCl, pH 8.0 at 25C
0.84
-
N-alpha-benzoyl-L-arginine-p-nitroanilide
-
isozyme TLE I, at 37C, pH 8.0
0.98
-
N-alpha-benzoyl-L-arginine-p-nitroanilide
-
isozyme TLE II, at 37C, pH 8.0
0.56
-
N-alpha-benzoyl-L-Lys-4-nitroanilide
-
recombinant enzyme, in 100 mM Tris-HCl, pH 8.0 at 25C
1.04
-
N-alpha-benzoyl-L-Lys-4-nitroanilide
Q54137
recombinant enzyme, in 100 mM Tris-HCl, pH 8.0 at 25C
66.7
-
N-alpha-p-tosyl-L-arginine methyl ester
-
trypsin B, at pH 8.0 and 30C
80
-
N-alpha-p-tosyl-L-arginine methyl ester
-
trypsin A, at pH 8.0 and 30C
0.8
-
N-benzoyl-DL-Arg-4-nitroanilide
-
-
1.34
-
N-benzoyl-DL-arginine 4-nitroanilide
-
pH 8.0, 25C
1.56
-
N-benzoyl-DL-arginine 4-nitroanilide
-
pH 9.0, 25C
2.32
-
N-benzoyl-DL-arginine 4-nitroanilide
-
pH 8.0, 25C
2.62
-
N-benzoyl-DL-arginine 4-nitroanilide
-
pH 8.5, 25C
4.71
-
N-benzoyl-DL-arginine 4-nitroanilide
-
pH 8.5, 60C
3.8
-
N-benzoyl-DL-arginine-4-nitroanilide
P00775
-
6.1
-
N-benzoyl-L-arginine-p-nitroanilide
-
in 67 mM sodium phosphate buffer, pH 7.6, at 25C
23.5
-
N-benzoyl-L-isoleucyl-L-glutamyl-glycyl-L-arginine-4-nitroanilide
-
isozyme trypsin-1, in 50 mM Tris, pH 8.0, 100 mM NaCl, 6 mM CaCl2
-
32.7
-
N-benzoyl-L-isoleucyl-L-glutamyl-glycyl-L-arginine-4-nitroanilide
-
isozyme trypsin-2, in 50 mM Tris, pH 8.0, 100 mM NaCl, 6 mM CaCl2
-
70.5
-
N-benzoyl-L-isoleucyl-L-glutamyl-glycyl-L-arginine-4-nitroanilide
-
isozyme trypsin-4, in 50 mM Tris, pH 8.0, 100 mM NaCl, 6 mM CaCl2
-
18.3
-
N-benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide
-
pH 6.0, 25C
48.9
-
N-benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide
-
pH 9.0, 25C
53.1
-
N-benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide
-
pH 8.0, 25C
54.5
-
N-benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide
-
pH 7.1, 25C
110
-
N-carbobenzyloxy-Gly-Pro-Arg-p-nitroanilide
-
mutant enzyme R193G, in 50 mM Tricine, 10 mM CaCl2, pH 8.0 at 20C
124
-
N-carbobenzyloxy-Gly-Pro-Arg-p-nitroanilide
-
mutant enzyme R193A, in 50 mM Tricine, 10 mM CaCl2, pH 8.0 at 20C
133
-
N-carbobenzyloxy-Gly-Pro-Arg-p-nitroanilide
-
mutant enzyme R193Y, in 50 mM Tricine, 10 mM CaCl2, pH 8.0 at 20C
158
-
N-carbobenzyloxy-Gly-Pro-Arg-p-nitroanilide
-
wild type enzyme, in 50 mM Tricine, 10 mM CaCl2, pH 8.0 at 20C
162
-
N-carbobenzyloxy-Gly-Pro-Arg-p-nitroanilide
-
mutant enzyme R193F, in 50 mM Tricine, 10 mM CaCl2, pH 8.0 at 20C
59.15
-
Nalpha-4-tosyl-L-arginine methyl ester
-
pH 8.5, 25C
210
-
Nalpha-4-tosyl-L-arginine methyl ester
-
pH 8.5, 45C
0.355
-
Nalpha-benzoyl-DL-Arg-p-nitroanilide
-
anionic trypsin
0.473
-
Nalpha-benzoyl-DL-Arg-p-nitroanilide
-
cationic trypsin 1
1
-
Nalpha-benzoyl-DL-Arg-p-nitroanilide
-
cationic trypsin 2
2.76
-
Nalpha-benzoyl-DL-arginine 4-nitroanilide
-
pH 10.5, 25C
1.21
-
Nalpha-benzoyl-DL-arginine-4-nitroanilide
-
isozyme trypsin B, pH 9.0, 25C
2.24
-
Nalpha-benzoyl-DL-arginine-4-nitroanilide
-
isozyme trypsin A, pH 9.0, 25C
5.76
-
Nalpha-benzoyl-DL-arginine-4-nitroanilide
-
isozyme trypsin C, pH 9.0, 25C
2.12
-
Nalpha-benzoyl-L-Arg-4-nitroanilide
-
pH 8.0, 25C
0.013
-
Nalpha-benzyloxycarbonyl-Lys-p-nitrophenyl ester
-
without addition of methanol
-
0.043
-
Nalpha-benzyloxycarbonyl-Lys-p-nitrophenyl ester
-
in presence of 10% methanol
-
0.052
-
Nalpha-benzyloxycarbonyl-Lys-p-nitrophenyl ester
-
in presence of 20% methanol
-
0.07
-
Nalpha-benzyloxycarbonyl-Lys-p-nitrophenyl ester
-
in presence of 30% methanol
-
0.13
-
Nalpha-benzyloxycarbonyl-Lys-p-nitrophenyl ester
-
in presence of 50% methanol
-
200
-
Nalpha-p-tosyl-L-Arg methyl ester
-
pH 8.0, 30C
33000
-
o-aminobenzoyl-AAGRGAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.0067
-
o-aminobenzoyl-AAGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.54
-
o-aminobenzoyl-AAGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
1.2
-
o-aminobenzoyl-AAGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
3800
-
o-aminobenzoyl-ADGRGAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.0014
-
o-aminobenzoyl-ADGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.013
-
o-aminobenzoyl-ADGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
13300
-
o-aminobenzoyl-AEGRGAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.0045
-
o-aminobenzoyl-AEGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.064
-
o-aminobenzoyl-AEGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.08
-
o-aminobenzoyl-AEGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
30000
-
o-aminobenzoyl-AFGRGAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.0072
-
o-aminobenzoyl-AFGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
1.2
-
o-aminobenzoyl-AFGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.00022
-
o-aminobenzoyl-AGARGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.74
-
o-aminobenzoyl-AGARGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
52
-
o-aminobenzoyl-AGARGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.00004
-
o-aminobenzoyl-AGDRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.03
-
o-aminobenzoyl-AGDRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
5.9
-
o-aminobenzoyl-AGDRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
330000
-
o-aminobenzoyl-AGERGAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.00004
-
o-aminobenzoyl-AGERGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.09
-
o-aminobenzoyl-AGERGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
1.4
-
o-aminobenzoyl-AGERGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.00022
-
o-aminobenzoyl-AGFRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.43
-
o-aminobenzoyl-AGFRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
26
-
o-aminobenzoyl-AGFRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.00004
-
o-aminobenzoyl-AGGKGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
1.4
-
o-aminobenzoyl-AGGKGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
1.7
-
o-aminobenzoyl-AGGKGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
630
-
o-aminobenzoyl-AGGKGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
240000
-
o-aminobenzoyl-AGGRAAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
123000
-
o-aminobenzoyl-AGGRDAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
133000
-
o-aminobenzoyl-AGGREAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
217000
-
o-aminobenzoyl-AGGRFAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
200000
-
o-aminobenzoyl-AGGRGAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.00011
-
o-aminobenzoyl-AGGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.9
-
o-aminobenzoyl-AGGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
2.7
-
o-aminobenzoyl-AGGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
820
-
o-aminobenzoyl-AGGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.00014
-
o-aminobenzoyl-AGGRGDGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.00013
-
o-aminobenzoyl-AGGRGEGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.0034
-
o-aminobenzoyl-AGGRGFGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.00029
-
o-aminobenzoyl-AGGRGGGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.00063
-
o-aminobenzoyl-AGGRGHGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.0007
-
o-aminobenzoyl-AGGRGIGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.00043
-
o-aminobenzoyl-AGGRGKGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.00068
-
o-aminobenzoyl-AGGRGLGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.00063
-
o-aminobenzoyl-AGGRGMGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.00027
-
o-aminobenzoyl-AGGRGNGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.00015
-
o-aminobenzoyl-AGGRGQGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.00034
-
o-aminobenzoyl-AGGRGSGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.0007
-
o-aminobenzoyl-AGGRGTGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.00078
-
o-aminobenzoyl-AGGRGVGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.00145
-
o-aminobenzoyl-AGGRGYGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
183000
-
o-aminobenzoyl-AGGRHAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
133000
-
o-aminobenzoyl-AGGRIAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
230000
-
o-aminobenzoyl-AGGRKAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
138000
-
o-aminobenzoyl-AGGRLAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
200000
-
o-aminobenzoyl-AGGRMAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
300000
-
o-aminobenzoyl-AGGRNAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
230000
-
o-aminobenzoyl-AGGRQAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
400000
-
o-aminobenzoyl-AGGRSAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
200000
-
o-aminobenzoyl-AGGRTAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
133000
-
o-aminobenzoyl-AGGRVAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
167000
-
o-aminobenzoyl-AGGRYAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
630000
-
o-aminobenzoyl-AGHRGAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.00027
-
o-aminobenzoyl-AGHRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.08
-
o-aminobenzoyl-AGHRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
14
-
o-aminobenzoyl-AGHRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.00022
-
o-aminobenzoyl-AGIRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.38
-
o-aminobenzoyl-AGIRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
12
-
o-aminobenzoyl-AGIRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
117000
-
o-aminobenzoyl-AGKRGAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.00072
-
o-aminobenzoyl-AGKRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.4
-
o-aminobenzoyl-AGKRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
41
-
o-aminobenzoyl-AGKRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
130000
-
o-aminobenzoyl-AGLRGAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.00017
-
o-aminobenzoyl-AGLRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.26
-
o-aminobenzoyl-AGLRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
19
-
o-aminobenzoyl-AGLRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.00015
-
o-aminobenzoyl-AGMRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.34
-
o-aminobenzoyl-AGMRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
17
-
o-aminobenzoyl-AGMRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
400000
-
o-aminobenzoyl-AGNRGAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.00017
-
o-aminobenzoyl-AGNRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.08
-
o-aminobenzoyl-AGNRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
140
-
o-aminobenzoyl-AGNRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.00014
-
o-aminobenzoyl-AGQRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.27
-
o-aminobenzoyl-AGQRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
30
-
o-aminobenzoyl-AGQRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
910000
-
o-aminobenzoyl-AGSRGAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.00011
-
o-aminobenzoyl-AGSRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.16
-
o-aminobenzoyl-AGSRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
14
-
o-aminobenzoyl-AGSRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
410000
-
o-aminobenzoyl-AGTRGAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.00011
-
o-aminobenzoyl-AGTRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.13
-
o-aminobenzoyl-AGTRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.00015
-
o-aminobenzoyl-AGVRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.4
-
o-aminobenzoyl-AGVRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
26
-
o-aminobenzoyl-AGVRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.00022
-
o-aminobenzoyl-AGYRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.3
-
o-aminobenzoyl-AGYRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
11
-
o-aminobenzoyl-AGYRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
26700
-
o-aminobenzoyl-AHGRGAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.004
-
o-aminobenzoyl-AHGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
20000
-
o-aminobenzoyl-AIGRGAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.0048
-
o-aminobenzoyl-AIGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.26
-
o-aminobenzoyl-AIGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.37
-
o-aminobenzoyl-AIGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
41000
-
o-aminobenzoyl-AKGRGAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.0055
-
o-aminobenzoyl-AKGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.17
-
o-aminobenzoyl-AKGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
2.1
-
o-aminobenzoyl-AKGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
33000
-
o-aminobenzoyl-ALGRGAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.0052
-
o-aminobenzoyl-ALGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.12
-
o-aminobenzoyl-ALGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.95
-
o-aminobenzoyl-ALGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
41000
-
o-aminobenzoyl-AMGRGAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.005
-
o-aminobenzoyl-AMGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.22
-
o-aminobenzoyl-AMGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
1.9
-
o-aminobenzoyl-AMGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
16300
-
o-aminobenzoyl-ANGRGAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.0043
-
o-aminobenzoyl-ANGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.83
-
o-aminobenzoyl-ANGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
50000
-
o-aminobenzoyl-AQGRGAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.0068
-
o-aminobenzoyl-AQGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.19
-
o-aminobenzoyl-AQGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
1.4
-
o-aminobenzoyl-AQGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
31700
-
o-aminobenzoyl-ASGRGAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.005
-
o-aminobenzoyl-ASGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.013
-
o-aminobenzoyl-ASGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
16500
-
o-aminobenzoyl-ATGRGAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.006
-
o-aminobenzoyl-ATGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
21700
-
o-aminobenzoyl-AVGRGAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.0047
-
o-aminobenzoyl-AVGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.57
-
o-aminobenzoyl-AVGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.9
-
o-aminobenzoyl-AVGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
31700
-
o-aminobenzoyl-AYGRGAGQ-(2,3-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30C
0.005
-
o-aminobenzoyl-AYGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.013
-
o-aminobenzoyl-AYGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30C in 100 mM Tris-HCl buffer pH 8.5
0.00167
-
succinyl-Ala-Ala-Pro-Arg-7-amido-4-methylcoumarin
-
pH 8.0, 37C, mutant enzyme D189S/DELTA223
91
-
succinyl-Ala-Ala-Pro-Arg-7-amido-4-methylcoumarin
-
pH 8.0, 37C, wild-type enzyme
95
-
succinyl-Ala-Ala-Pro-Arg-p-nitroanilide
-
wild-type enzyme, pH 7
144
-
succinyl-Ala-Ala-Pro-Arg-p-nitroanilide
-
wild-type enzyme, pH 9
145
-
succinyl-Ala-Ala-Pro-Arg-p-nitroanilide
-
wild-type enzyme, pH 10
163
-
succinyl-Ala-Ala-Pro-Arg-p-nitroanilide
-
wild-type enzyme, pH 8
0.000167
-
succinyl-Ala-Ala-Pro-Leu-7-amido-4-methylcoumarin
-
pH 8.0, 37C, wild-type enzyme
0.00217
-
succinyl-Ala-Ala-Pro-Leu-7-amido-4-methylcoumarin
-
pH 8.0, 37C, mutant enzyme D189S/DELTA223
0.004
-
succinyl-Ala-Ala-Pro-Lys-7-amido-4-methylcoumarin
-
pH 8.0, 37C, mutant enzyme D189S/DELTA223
73
-
succinyl-Ala-Ala-Pro-Lys-7-amido-4-methylcoumarin
-
pH 8.0, 37C, wild-type enzyme
121.2
-
succinyl-Ala-Ala-Pro-Lys-7-amido-4-methylcoumarin
-
pH 8.0, 37C, wild-type enzyme
40
-
succinyl-Ala-Ala-Pro-Lys-p-nitroanilide
-
wild-type enzyme, pH 7
59
-
succinyl-Ala-Ala-Pro-Lys-p-nitroanilide
-
wild-type enzyme, pH 9
64
-
succinyl-Ala-Ala-Pro-Lys-p-nitroanilide
-
wild-type enzyme, pH 10
74
-
succinyl-Ala-Ala-Pro-Lys-p-nitroanilide
-
wild-type enzyme, pH 8
0.004
-
succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin
-
pH 8.0, 37C, mutant enzyme D189S/DELTA223
0.0063
-
succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin
-
pH 8.0, 37C, wild-type enzyme
0.000167
-
succinyl-Ala-Ala-Pro-Trp-7-amido-4-methylcoumarin
-
pH 8.0, 37C, mutant enzyme D189S/DELTA223
0.00067
-
succinyl-Ala-Ala-Pro-Tyr-7-amido-4-methylcoumarin
-
pH 8.0, 37C, wild-type enzyme
0.08
-
succinyl-Ala-Ala-Pro-Tyr-7-amido-4-methylcoumarin
-
pH 8.0, 37C, mutant enzyme D189S/DELTA223
1.41
-
tert-butoxycarbonyl-L-Gln-L-Ala-L-Arg-7-amido-4-methylcoumarin
-
in 10 mM Tris, 20 mM CaCl2, pH 7.4, at 37C
14.7
-
tert-butyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin
-
pH 8.0, 30C
41.3
-
Tos-L-Arg-OMe
-
isozyme ST-1, at 5C
46.2
-
Tos-L-Arg-OMe
-
at 5C
61.7
-
Tos-L-Arg-OMe
-
isozyme ST-2, at 5C
64.5
-
Tos-L-Arg-OMe
-
isozyme ST-1, at 15C
68.4
-
Tos-L-Arg-OMe
-
isozyme ST-3, at 5C
69.2
-
Tos-L-Arg-OMe
-
at 15C
94.3
-
Tos-L-Arg-OMe
-
isozyme ST-1, at 25C; isozyme ST-1, at 35C
105
-
Tos-L-Arg-OMe
-
isozyme ST-2, at 15C
109
-
Tos-L-Arg-OMe
-
at 25C
139
-
Tos-L-Arg-OMe
-
at 35C
154
-
Tos-L-Arg-OMe
-
isozyme ST-3, at 15C
159
-
Tos-L-Arg-OMe
-
isozyme ST-3, at 25C
189
-
Tos-L-Arg-OMe
-
isozyme ST-3, at 35C
200
-
Tos-L-Arg-OMe
-
isozyme ST-2, at 35C
234
-
Tos-L-Arg-OMe
-
isozyme ST-2, at 25C
1.7
-
tosyl-Arg methyl ester
-
gamma-trypsin
76
-
tosyl-Arg methyl ester
-
alpha-trypsin
28
-
tosyl-Gly-Pro-Arg-4-nitroanilide
salmon
-
pH 8.3
30.4
-
tosyl-Gly-Pro-Arg-4-nitroanilide
salmon
-
pH 8.3
88
-
tosyl-Gly-Pro-Arg-4-nitroanilide
-
pH 8.3
0.52
0.7
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin
-
pH 7.6, wild-type enzyme
2.7
-
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin
-
pH 7.6, recombinant enzyme
8
-
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin
-
pH 7.6, mutant enzyme T190P
38.33
-
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin
-
pH 7.6, mutant enzyme T190V
82.5
-
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin
-
pH 7.6, mutant enzyme T190A
0.04
1.97
tosyl-Gly-Pro-Lys-7-amido-4-methylcoumarin
-
pH 7.6, mutant enzyme T190P
0.5
-
tosyl-Gly-Pro-Lys-7-amido-4-methylcoumarin
-
pH 7.6, mutant enzyme T190V
3.2
-
tosyl-Gly-Pro-Lys-7-amido-4-methylcoumarin
-
pH 7.6, mutant enzyme T190A
8.78
-
tosyl-Gly-Pro-Lys-7-amido-4-methylcoumarin
-
pH 7.6, mutant enzyme T190P
3.86
-
Z-Gly-Pro-Arg-p-nitroanilide
-
mutant enzyme R193G/G142A, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20C
20
50
Z-Gly-Pro-Arg-p-nitroanilide
-
wild type enzyme, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20C
29.2
-
Z-Gly-Pro-Arg-p-nitroanilide
-
mutant enzyme R193G/G184A, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20C
51.5
-
Z-Gly-Pro-Arg-p-nitroanilide
-
mutant enzyme R193G/G19A, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20C
54.1
-
Z-Gly-Pro-Arg-p-nitroanilide
-
mutant enzyme R193G, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20C
83.6
-
Z-Gly-Pro-Arg-p-nitroanilide
-
mutant enzyme R193A, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20C
88.2
-
Z-Gly-Pro-Arg-p-nitroanilide
-
wild type enzyme, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20C
24.9
-
MeOSuc-Ala-Arg 4-nitroanilide
-
pH 8.0, 25C
additional information
-
additional information
-
the mutations C191A and C220A decrease the activity of trypsin by 20-200fold as measured by the ratio of turnover number to Km-value for the hydrolysis of amide substrates, ester hydrolysis is decreased by less than 10fold
-
additional information
-
additional information
-
influence of pH-value on turnover number of wild-type and mutant enzymes
-
kcat/KM VALUE [1/mMs-1]
kcat/KM VALUE [1/mMs-1] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2520
-
Abz-ERFK-EDDnp
-
pH 8.0, 25C
302530
16680
-
Abz-FRFK-EDDnp
-
pH 8.0, 25C
302524
1960
-
Abz-GRFK-EDDnp
-
pH 8.0, 25C
302522
3110
-
Abz-HRFK-EDDnp
-
pH 8.0, 25C
302532
91580
-
Abz-KRFK-EDDnp
-
pH 8.0, 25C
302531
52600
-
Abz-LRFK-EDDnp
-
pH 8.0, 25C
302525
4120
-
Abz-NRFK-EDDnp
-
pH 8.0, 25C
302527
1420
-
Abz-QRFK-EDDnp
-
pH 8.0, 25C
302528
290
-
Abz-RDFK-EDDnp
-
pH 8.0, 25C
302517
580
-
Abz-REFK-EDDnp
-
pH 8.0, 25C
302518
570
-
Abz-RGFK-EDDnp
-
pH 8.0, 25C
302510
4490
-
Abz-RHFK-EDDnp
-
pH 8.0, 25C
302520
3380
-
Abz-RNFK-EDDnp
-
pH 8.0, 25C
302515
30
-
Abz-RPFK-EDDnp
-
pH 8.0, 25C
302512
1800
-
Abz-RQFK-EDDnp
-
pH 8.0, 25C
302516
16460
-
Abz-RRFK-EDDnp
-
pH 8.0, 25C
302519
16300
-
Abz-RSFK-EDDnp
-
pH 8.0, 25C
302513
5630
-
Abz-RTFK-EDDnp
-
pH 8.0, 25C
302521
250
-
Abz-RVFK-EDDnp
-
pH 8.0, 25C
302511
6400
-
Abz-RYFK-EDDnp
-
pH 8.0, 25C
302514
16220
-
Abz-VRFK-EDDnp
-
pH 8.0, 25C
302523
1940
-
Abz-WRFK-EDDnp
-
pH 8.0, 25C
302529
4980
-
Abz-YRFK-EDDnp
-
pH 8.0, 25C
302526
0.0796
-
benzyloxycarbonyl-L-glutaminyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide
-
pH 8.0, 37C, trypsin A
0
0.1084
-
benzyloxycarbonyl-L-glutaminyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide
-
pH 8.0, 37C, trypsin B
0
0.0163
-
benzyloxycarbonyl-L-leucyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide
-
pH 8.0, 37C, trypsin A
0
0.0417
-
benzyloxycarbonyl-L-leucyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide
-
pH 8.0, 37C, trypsin B
0
0.0141
-
benzyloxycarbonyl-L-leucyl-L-lysyl-L-arginine-4-methylcoumaryl-7-amide
-
pH 8.0, 37C, trypsin A
0
0.0149
-
benzyloxycarbonyl-L-leucyl-L-lysyl-L-arginine-4-methylcoumaryl-7-amide
-
pH 8.0, 37C, trypsin B
0
0.0642
-
benzyloxycarbonyl-L-phenylalaninyl-L-seryl-L-arginine-4-methylcoumaryl-7-amide
-
pH 8.0, 37C, trypsin A
0
0.0926
-
benzyloxycarbonyl-L-phenylalaninyl-L-seryl-L-arginine-4-methylcoumaryl-7-amide
-
pH 8.0, 37C, trypsin B
0
0.0106
-
benzyloxycarbonyl-L-valyl-L-leucyl-L-lysine-4-methylcoumaryl-7-amide
-
pH 8.0, 37C, trypsin A
0
0.0152
-
benzyloxycarbonyl-L-valyl-L-leucyl-L-lysine-4-methylcoumaryl-7-amide
-
pH 8.0, 37C, trypsin B
0
0.00945
-
benzyloxycarbonyl-L-valyl-L-prolyl-L-arginine-4-methylcoumaryl-7-amide
-
pH 8.0, 37C, trypsin A
0
0.0436
-
benzyloxycarbonyl-L-valyl-L-prolyl-L-arginine-4-methylcoumaryl-7-amide
-
pH 8.0, 37C, trypsin B
0
0.0019
-
benzyloxycarbonylglycyl-L-prolyl-L-arginine-4-methylcoumaryl-7-amide
-
wild-type enzyme, pH 8.0, 37C
0
0.0032
-
benzyloxycarbonylglycyl-L-prolyl-L-arginine-4-methylcoumaryl-7-amide
-
mutant L71Y, pH 8.0, 37C
0
0.0048
-
benzyloxycarbonylglycyl-L-prolyl-L-arginine-4-methylcoumaryl-7-amide
-
mutant L71Y/Q72R, pH 8.0, 37C
0
0.0054
-
benzyloxycarbonylglycyl-L-prolyl-L-arginine-4-methylcoumaryl-7-amide
-
mutant Y71L, pH 8.0, 37C
0
0.0419
-
benzyloxycarbonylglycyl-L-prolyl-L-arginine-4-methylcoumaryl-7-amide
-
wild-type enzyme, pH 8.0, 37C
0
1130
-
MeOSuc-Ala-Ala-Ala-Arg 4-nitroanilide
-
pH 8.0, 25C
302537
2585
-
MeOSuc-Ala-Ala-Arg 4-nitroanilide
-
pH 8.0, 25C
302536
1383
-
MeOSuc-Ala-Arg 4-nitroanilide
-
pH 8.0, 25C
302535
12
-
N-benzoyl-DL-arginine 4-nitroanilide
-
pH 9.0, 25C
208679
26.8
-
N-benzoyl-DL-arginine 4-nitroanilide
-
pH 8.0, 25C
208679
36.3
-
N-benzoyl-DL-arginine 4-nitroanilide
-
pH 8.0, 25C
208679
538.2
-
N-benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide
-
pH 6.0, 25C
302534
3759
-
N-benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide
-
pH 7.1, 25C
302534
5433
-
N-benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide
-
pH 9.0, 25C
302534
7590
-
N-benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide
-
pH 8.0, 25C
302534
1400
-
Nalpha-4-tosyl-L-arginine methyl ester
-
pH 8.5, 45C
302533
40.6
-
Nalpha-benzoyl-DL-arginine 4-nitroanilide
-
pH 10.5, 25C
302538
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.0001
-
(2R,4R)-4-phenyl-1-[Nalpha-(7-methoxy-2-naphthalenesulfonyl)-L-arginyl]-2-piperidinecarboxylic acid
-
pH 8.1, 37C
0.0000247
-
2,7-bis(4-amidinobenzylidene)-cycloheptan-1-one
-
variant X(99/175/190)rT containing the 99-loop the 175-loop (Ser172, Ser173, Phe174, Ile175) and Ala190 of factor Xa
0.000054
-
2,7-bis(4-amidinobenzylidene)-cycloheptan-1-one
-
variant X99rT containing the 99-loop (Glu97,Tyr99) of factor Xa
0.000122
-
2,7-bis(4-amidinobenzylidene)-cycloheptan-1-one
-
native enzyme
0.0003
-
2,7-bis(4-amidinobenzylidene)cycloheptan-1-one
-
pH 8.0, mutant enzyme S190A
0.0004
-
2,7-bis(4-amidinobenzylidene)cycloheptan-1-one
-
pH 8.0, wild-type enzyme
0.0007
-
2,7-bis(4-amidinobenzylidene)cycloheptan-1-one
-
pH 8.0, mutant enzyme E97N/Y99L
0.0009
-
2,7-bis(4-amidinobenzylidene)cycloheptan-1-one
-
pH 8.0, mutant enzyme E97N/Y99L/S190A
0.00000015
-
acyclic SFTI
-
pH not specified in the publication, temperature not specified in the publication
0.000003
-
Apios Americana trypsin inhibitor
-
-
-
0.0004
-
AVNIPFKVHFRCKAAFC
-
in 50 mM Tris-HCl, pH 7.8, at 37C
0.0027
-
Benzamidine
-
pH 7.6, recombinant enzyme
0.0048
-
Benzamidine
-
pH 7.6, mutant enzyme T190S
0.00651
-
Benzamidine
-
native enzyme
0.0099
-
Benzamidine
-
pH 7.6, mutant enzyme T190A
0.0164
-
Benzamidine
-
pH 7.6, mutant enzyme T190P
0.0322
-
Benzamidine
-
variant X99rT containing the 99-loop (Glu97, Tyr99) of factor Xa
0.034
-
Benzamidine
-
pH 8.0, mutant enzyme S190A
0.036
-
Benzamidine
-
pH 8.0, mutant enzyme E97N/Y99L
0.039
-
Benzamidine
-
pH 8.0, wild-type enzyme
0.053
-
Benzamidine
-
pH 8.0, mutant enzyme E97N/Y99L/S190A
0.143
-
Benzamidine
-
variant X(99/175/190)rT containing the 99-loop the 175-loop (Ser172,Ser173, Phe174, Ile175) and Ala190 of factor Xa
0.197
-
Benzamidine
-
pH 7.6, mutant enzyme T190V
0.0018
-
bis(m-amidinosalicylidene-L-alaninato)iron(III)
-
-
0.0016
-
bis(p-amidinosalicylidene-L-alaninato)iron(III)
-
-
-
0.0000029
-
Bovine pancreatic trypsin inhibitor
-
recombinant inhibitor from Pichia pastoris, in 50 mM Tris-HCl, 150 mM NaCl, 5 mM CaCl2, pH 7.5 containing 0.1% (w/v) bovine serum albumin, at 25C
-
0.000000063
-
cMCoTI-II
-
pH not specified in the publication, temperature not specified in the publication
-
0.0000031
-
cnispin
-
pH 8.0, 37C
-
0.00041
-
CRC 220
-
-
0.00000008
-
cyclic SFTI
-
pH not specified in the publication, temperature not specified in the publication
0.0000017
-
DMTI-II
-
-
-
0.0000003
-
DOTA-SFTI
-
pH not specified in the publication, temperature not specified in the publication
0.000013
-
Kunitz domain 1 of tissue factor pathway inhibitor-2
-
-
-
0.000849
-
LEKTI
-
pH 7.4, 25C
0.000031
-
Leupeptin
-
in 10 mM Tris, 20 mM CaCl2, pH 7.4, at 37C
0.026
-
Nafamostat
-
-
0.000044
-
Nalpha-(2-naphthylsulfonyl)-L-3-amidino-phenylalanine-4'-acetyl-piperazide
-
-
0.000042
-
Nalpha-(2-naphthylsulfonyl)-L-3-amidino-phenylalanine-4'-methylpiperidide
-
-
0.00066
-
Nalpha-(2-naphthylsulfonyl)-L-3-amidino-phenylalanine-D-pipecolic acid
-
-
0.0011
-
Nalpha-(2-naphthylsulfonyl)-L-3-amidino-phenylalanine-D-pipecolic acid methyl ester
-
-
0.00022
-
Nalpha-(2-naphthylsulfonyl)-L-3-amidino-phenylalanine-isopecotinic acid
-
-
0.000021
-
Nalpha-(2-naphthylsulfonyl)-L-3-amidino-phenylalanine-isopecotinic acid methylester
-
-
0.00012
-
Nalpha-(2-naphthylsulfonyl)-L-3-amidino-phenylalanine-piperazide
-
-
0.00000805
-
Nalpha-tosylglycyl-3-amidino-DL-phenylalanine-methyl ester
-
native enzyme
0.00152
-
Nalpha-tosylglycyl-3-amidino-DL-phenylalanine-methyl ester
-
variant X99rT containing the 99-loop (Glu97, Tyr99) of factor Xa
0.00871
-
Nalpha-tosylglycyl-3-amidino-DL-phenylalanine-methyl ester
-
variant X(99/175/190)rT containing the 99-loop the 175-loop (Ser172, Ser173, Phe174, Ile175) and Ala190 of factor Xa
0.0116
-
napsagatran
-
-
0.03
-
napsagatran ethylester
-
-
0.00000043
-
oMCoTI-II
-
pH not specified in the publication, temperature not specified in the publication
-
0.0042
-
p-amidinosalicylidene-L-alaninato(aqua)copper(II)
-
-
0.00004
-
Plathymenia foliolosa trypsin inhibitor
-
at 37C, in Tris-HCl buffer (100 mM), pH 8.0
-
0.000000014
-
Putranjiva roxburghii trypsin inhibitor
-
in 50 mM Tris-HCl buffer, pH 8.0 containing 20 mM CaCl2 and 0.001 N HCl, at 30C
-
0.0023
-
Spinacia oleracea trypsin inhibitor
-
in 0.1 M Tris_HCl (pH 8.0) at 37C
-
0.0000188
-
SVIGCWTKSIPPRPCFVK-amide
-
in 10 mM phosphate buffer, pH 7.4, containing 2.7 mM KCl and 137 mM NaCl, at 37C
0.0000542
-
SVIGCWTRSIPPRPCFVK-amide
-
in 10 mM phosphate buffer, pH 7.4, containing 2.7 mM KCl and 137 mM NaCl, at 37C
0.0003098
-
Vigna mungo trypsin inhibitor
-
pH 8.2, 37C
-
0.0023
-
[4-(6-chloro-naphthalene-2-sulfonyl)-piperazin-1-yl]-(3,4,5-6-tetrahydro-[2H-1,4']bipyridinyl-4yl)-methanone
-
pH 8.0, mutant enzyme E97N/Y99L
0.0067
-
[4-(6-chloro-naphthalene-2-sulfonyl)-piperazin-1-yl]-(3,4,5-6-tetrahydro-[2H-1,4']bipyridinyl-4yl)-methanone
-
pH 8.0, mutant enzyme E97N/Y99L/S190A
0.008
-
[4-(6-chloro-naphthalene-2-sulfonyl)-piperazin-1-yl]-(3,4,5-6-tetrahydro-[2H-1,4']bipyridinyl-4yl)-methanone
-
pH 8.0, mutant enzyme S190A
0.0134
-
[4-(6-chloro-naphthalene-2-sulfonyl)-piperazin-1-yl]-(3,4,5-6-tetrahydro-[2H-1,4']bipyridinyl-4yl)-methanone
-
pH 8.0, wild-type enzyme
0.00605
-
[4-(6-chloro-naphthalene-2-sulphonyl)piperazin-1-yl]-(3,4,5,6-tetrahydro-[2H-1,4']bipyridinyl-4-yl)-methanone
-
variant X(99/175/190)rT containing the 99-loop the 175-loop (Ser172, Ser173, Phe174, Ile175) and Ala190 of factor Xa
0.0256
-
[4-(6-chloro-naphthalene-2-sulphonyl)piperazin-1-yl]-(3,4,5,6-tetrahydro-[2H-1,4']bipyridinyl-4-yl)-methanone
-
native enzyme
0.0445
-
[4-(6-chloro-naphthalene-2-sulphonyl)piperazin-1-yl]-(3,4,5,6-tetrahydro-[2H-1,4']bipyridinyl-4-yl)-methanone
-
variant X99rT containing the 99-loop (Glu97,Tyr99) of factor Xa
0.00001
-
[Arg5]-SFTI-1
-
pH 8.1, 37C
0.0000001
-
[Lys5]-SFTI-1
-
pH 8.1, 37C
0.00022
-
[Phe5]-SFTI-1
-
pH 8.1, 37C
0.0028
-
m-amidinosalicylidene-L-alaninato(aqua)copper(II)
-
-
additional information
-
additional information
-
enzyme inhibition kinetic analysis of wild-type inhibitor and mutants, Lys33GP, Lys33K/F, and Lys16 peptide, overview
-
additional information
-
additional information
-
Glycine max trypsin inhibitor inhibition kinetics, overview
-
additional information
-
additional information
-
pseudo-first-order inhibition kinetics for trypsin, overview
-
IC50 VALUE [mM]
IC50 VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.0003
-
1-[N2-[(7-methoxynaphthalen-2-yl)sulfonyl]-L-arginyl]-4-phenylpiperidine-2-carboxylic acid
-
IC50: 0.0003 mM
0.04
-
antipain
-
IC50: 0.04 mM
0.0002
-
Leupeptin
-
IC50: 0.0002 mM
0.0013
-
lily bulb trypsin inhibitor
-
in 50 M Tris-HCl (pH 8.0), at 25C
-
0.00008
-
mustard trypsin inhibitor 2
-
in 100 mM Tris-HCl, pH 8
-
0.00003
-
nafamostat mesilate
-
IC50: 0.00003 mM
0.019
-
small glossy black soybean trypsin inhibitor
-
in 0.1 M Tris-HCl (pH 7.4), at 37C
-
0.000006
-
soybean Kunitz trypsin inhibitor
-
in 100 mM Tris-HCl, pH 8
-
0.000009
-
Soybean trypsin inhibitor
-
IC50: 0.000009 mM
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.11
-
-
crude extract, using N-alpha-p-tosyl-L-arginine methyl ester as a substrate, at pH 8.0 and 30C
0.23
-
-
clarified extract, at 25C
0.34
-
P00775
after 1fold purification, using N-benzoyl-DL-arginine-4-nitroanilide as a substrate
0.47
-
-
pH 8.2, 25C
0.52
-
-
crude extract, at 37C, pH 8.0
0.6
-
-
crude enzyme
0.897
-
-
form B
0.9397
-
-
form A
3.12
-
-
purified enzyme, pH 8.0, 25C
4.03
-
-
purified enzyme, pH 10.5, 25C
4.85
-
-
aT-I
5.2
-
-
purified enzyme, pH 9.0, 25C
5.56
-
-
isozyme ST-3, after 24.2fold purification, at 25C
6.23
-
-
isozyme ST-2, after 27.1fold purification, at 25C
6.43
-
-
isozyme ST-1, after 28fold purification, at 25C
6.5
-
-
pH 8.0, 25C
7.4
-
-
Nalpha-benzoyl-DL-Arg-p-nitroanilide amidolysis
7.77
-
-
trypsin A after 70.6fold purification, using N-alpha-p-tosyl-L-arginine methyl ester as a substrate, at pH 8.0 and 30C
8.9
-
-
purified enzyme, pH 8.5, 45C
9.26
-
-
aT-II
9.42
-
P00775
after 27.6fold purification, using N-benzoyl-DL-arginine-4-nitroanilide as a substrate
10.07
-
-
trypsin B after 91.5fold purification, using N-alpha-p-tosyl-L-arginine methyl ester as a substrate, at pH 8.0 and 30C
18
-
-
purified enzyme, pH 8.0, 50C
21.65
-
-
purified enzyme, pH 8.5, 25C
23.12
-
-
purified native trypsin A, pH 9.0, 25C
24
-
-
purified enzyme, pH 8.0, 50C
35.94
-
-
purified native trypsin B, pH 9.0, 25C
36.15
-
-
pH 8.0, 30C
38.3
-
-
pH 8.0, 37C, trypsin B, substrate benzyloxycarbonyl-L-phenylalaninyl-L-seryl-L-arginine-4-methylcoumaryl-7-amide
43.24
-
-
purified enzyme, pH 8.0, 37C
50
-
-
after 83fold putrification
61.83
-
-
purified native trypsin C, pH 9.0, 25C
64
-
-
isozyme TLE I after 123fold purification, at 37C, pH 8.0
66.1
-
-
wild-type enzyme, pH 8.0, 37C, substrate benzyloxycarbonylglycyl-L-prolyl-L-arginine-4-methylcoumaryl-7-amide
68.1
-
-
pH 8.0, 37C, trypsin A, substrate benzyloxycarbonyl-L-phenylalaninyl-L-seryl-L-arginine-4-methylcoumaryl-7-amide
90.3
-
-
mutant L71Y, pH 8.0, 37C, substrate benzyloxycarbonylglycyl-L-prolyl-L-arginine-4-methylcoumaryl-7-amide
92
-
-
isozyme TLE II after 177fold purification, at 37C, pH 8.0
103
-
-
isoform T2, 35C
109
-
-
isoform T1, 35C
110.2
-
-
mutant L71Y/Q72R, pH 8.0, 37C, substrate benzyloxycarbonylglycyl-L-prolyl-L-arginine-4-methylcoumaryl-7-amide
115.6
-
-
N-benzoyl-L-Arg ethyl ester esterolysis
142.8
-
-
enzyme II
155.7
-
-
enzyme III
156
-
-
purified enzyme, pH 8.5, 60C
190.2
-
-
mutant Y71L, pH 8.0, 37C, substrate benzyloxycarbonylglycyl-L-prolyl-L-arginine-4-methylcoumaryl-7-amide
205
-
-
isoform T3, 35C
339
-
-
enzyme I
434.7
-
-
purified enzyme, pH 8.5, 60C
501.5
-
-
wild-type enzyme, pH 8.0, 37C, substrate benzyloxycarbonylglycyl-L-prolyl-L-arginine-4-methylcoumaryl-7-amide
529
875
-
partially purified native trypsin-like serine proteinases, pH 8.2, 25C
additional information
-
-
detection of enzyme hydrolytic activity by use of 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate coupled to a chymotryptic petide obtained from hemoglobin, on silica gel plate. Detection of 45 fmol of enzyme at 25C within 1 min
additional information
-
-
vmax-value is 5467 microgramms per min for free enzyme, 793 microgramms per min for immobilized enzyme
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7
8
-
hydrolysis of casein
7
-
-
-
7
-
Cambarus affinis
-
hydrolysis of tosyl-Arg methyl ester, enzyme immobilized on DEAE-Sephadex A-50 KT
7.5
11.5
-
with substrate N-benzoyl-DL-arginine 4-nitroanilide
7.5
9.5
-
-
7.5
-
-
protease activity assay at
7.6
-
-
assay at
7.6
-
-
assay at
7.8
-
-
assay at
7.8
-
-
esterase activity assay at
7.9
-
-
isoforms T1, T2
8
10
-
the optimum pH for digestion with immobilized trypsin on porous polymer monoliths is in a pH range 8-10 and comparable with that found for its soluble counterpart
8
9
-
-
8
-
-
around pH 8.0
8
-
-
assay at
8
-
-
assay at
8
-
-
assay at
8.1
-
-
assay at
8.1
-
-
hydrolysis of Nalpha-toluolsulfonyl-L-Arg methyl ester
8.2
-
-
assay at
8.2
-
-
assay at
8.2
-
-
assay at
8.2
-
-
assay at
8.3
-
-
-
8.3
-
-
assay at
8.5
-
-
assay at
8.5
-
-
assay at
8.5
-
Cambarus affinis
-
hydrolysis of tosyl-Arg methyl ester, free enzyme
8.6
-
-
recombinant enzyme from Zea may
8.7
-
-
native bovine trypsin
8.8
-
-
isoform T3
8.8
-
-
autodigestion assay at
9
10