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Literature summary for 3.4.21.4 extracted from

  • Chobert, J.M.; Briant, L.; Leonil, J.; Molle, D.; Haertle, T.
    Engineering of trypsin and its impact on beta-casein processing (1998), Nahrung, 42, 135-138.
    View publication on PubMed
Search for more literature for 3.4.21.4

Protein Variants

Protein Variants Comment Organism
K188F increase of Km for Arg and Lys containing substrates, cleavage of nearly 30 new peptide bonds in beta-casein compared to the wild-type enzyme Rattus norvegicus
K188W increase of Km for Arg and Lys containing substrates, 3-4fold decrease in turnover number Rattus norvegicus
K188Y increase of Km for Arg and Lys containing substrates, cleavage of nearly 30 new peptide bonds in beta-casein compared to the wild-type enzyme Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information influence of pH-value on Km-value of wild-type and mutant enzymes Rattus norvegicus
0.033
-
 succinyl-Ala-Ala-Pro-Arg-p-nitroanilide wild-type enzyme, pH 8 Rattus norvegicus
0.057
-
 succinyl-Ala-Ala-Pro-Arg-p-nitroanilide wild-type enzyme, pH 9 Rattus norvegicus
0.087
-
 succinyl-Ala-Ala-Pro-Arg-p-nitroanilide wild-type enzyme, pH 7 Rattus norvegicus
0.099
-
 succinyl-Ala-Ala-Pro-Arg-p-nitroanilide wild-type enzyme, pH 10 Rattus norvegicus
0.101
-
 succinyl-Ala-Ala-Pro-Lys-p-nitroanilide wild-type enzyme, pH 9 Rattus norvegicus
0.105
-
 succinyl-Ala-Ala-Pro-Lys-p-nitroanilide wild-type enzyme, pH 8 Rattus norvegicus
0.184
-
 succinyl-Ala-Ala-Pro-Lys-p-nitroanilide wild-type enzyme, pH 10 Rattus norvegicus
0.839
-
 succinyl-Ala-Ala-Pro-Lys-p-nitroanilide wild-type enzyme, pH 7 Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
casein + H2O beta-casein, mutant enzymes K188E and K188Y cleave nearly 30 new peptide bonds compared to wild-type enzyme Rattus norvegicus ?
-
 ?
succinyl-Ala-Ala-Pro-Arg-p-nitroanilide + H2O
-
 Rattus norvegicus ?
-
 ?
succinyl-Ala-Ala-Pro-Lys-p-nitroanilide + H2O
-
 Rattus norvegicus ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information influence of pH-value on turnover number of wild-type and mutant enzymes Rattus norvegicus
40
-
 succinyl-Ala-Ala-Pro-Lys-p-nitroanilide wild-type enzyme, pH 7 Rattus norvegicus
59
-
 succinyl-Ala-Ala-Pro-Lys-p-nitroanilide wild-type enzyme, pH 9 Rattus norvegicus
64
-
 succinyl-Ala-Ala-Pro-Lys-p-nitroanilide wild-type enzyme, pH 10 Rattus norvegicus
74
-
 succinyl-Ala-Ala-Pro-Lys-p-nitroanilide wild-type enzyme, pH 8 Rattus norvegicus
95
-
 succinyl-Ala-Ala-Pro-Arg-p-nitroanilide wild-type enzyme, pH 7 Rattus norvegicus
144
-
 succinyl-Ala-Ala-Pro-Arg-p-nitroanilide wild-type enzyme, pH 9 Rattus norvegicus
145
-
 succinyl-Ala-Ala-Pro-Arg-p-nitroanilide wild-type enzyme, pH 10 Rattus norvegicus
163
-
 succinyl-Ala-Ala-Pro-Arg-p-nitroanilide wild-type enzyme, pH 8 Rattus norvegicus