Literature summary for 3.4.21.4 extracted from

Cai, Q.F.; Jiang, Y.K.; Zhou, L.G.; Sun, L.C.; Liu, G.M.; Osatomi, K.; Cao, M.J.
Biochemical characterization of trypsins from the hepatopancreas of Japanese sea bass (Lateolabrax japonicus) (2011), Comp. Biochem. Physiol. B, 159, 183-189.

Search for more literature for 3.4.21.4

Inhibitors

Inhibitors	Comment	Organism	Structure
benzamidine	72.0% inhibition of trypsin A, 95.4% of trypsin B, at 1 mM. 88.8% inhibition of trypsin A, 97.6% of trypsin B at 0.1 mM,	Lateolabrax japonicus
E-64	18.3% inhibition of trypsin A, 11.1% of trypsin B, at 0.001 mM. 46.9% inhibition of trypsin A, 33.8% of trypsin B at 0.01 mM	Lateolabrax japonicus
additional information	no or poor inhibition of trypsin A and trypsin B by pepstatin at 0.001 mM, and EDTA and 1,10-phenathroline at 10 mM	Lateolabrax japonicus
Pefabloc SC	complete inhibition of trypsin A and B at 1 mM	Lateolabrax japonicus
PMSF	80.7% inhibition of trypsin A, 57.4% of trypsin B at 0.1 mM. 96.4% inhibition of trypsin A, 93.2% of trypsin B at 1 mM	Lateolabrax japonicus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00028	-	benzyloxycarbonyl-L-leucyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide	pH 8.0, 37°C, trypsin A	Lateolabrax japonicus
0.0003	-	benzyloxycarbonyl-L-valyl-L-leucyl-L-lysine-4-methylcoumaryl-7-amide	pH 8.0, 37°C, trypsin A	Lateolabrax japonicus
0.00036	-	benzyloxycarbonyl-L-leucyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide	pH 8.0, 37°C, trypsin B	Lateolabrax japonicus
0.00038	-	benzyloxycarbonyl-L-glutaminyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide	pH 8.0, 37°C, trypsin B	Lateolabrax japonicus
0.00039	-	benzyloxycarbonyl-L-leucyl-L-lysyl-L-arginine-4-methylcoumaryl-7-amide	pH 8.0, 37°C, trypsin A	Lateolabrax japonicus
0.00053	-	benzyloxycarbonyl-L-valyl-L-leucyl-L-lysine-4-methylcoumaryl-7-amide	pH 8.0, 37°C, trypsin B	Lateolabrax japonicus
0.00054	-	benzyloxycarbonyl-L-glutaminyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide	pH 8.0, 37°C, trypsin A	Lateolabrax japonicus
0.00058	-	benzyloxycarbonyl-L-valyl-L-prolyl-L-arginine-4-methylcoumaryl-7-amide	pH 8.0, 37°C, trypsin A	Lateolabrax japonicus
0.00059	-	benzyloxycarbonyl-L-valyl-L-prolyl-L-arginine-4-methylcoumaryl-7-amide	pH 8.0, 37°C, trypsin B	Lateolabrax japonicus
0.0007	-	benzyloxycarbonyl-L-phenylalaninyl-L-seryl-L-arginine-4-methylcoumaryl-7-amide	pH 8.0, 37°C, trypsin B	Lateolabrax japonicus
0.00082	-	benzyloxycarbonyl-L-leucyl-L-lysyl-L-arginine-4-methylcoumaryl-7-amide	pH 8.0, 37°C, trypsin B	Lateolabrax japonicus
0.00112	-	benzyloxycarbonyl-L-phenylalaninyl-L-seryl-L-arginine-4-methylcoumaryl-7-amide	pH 8.0, 37°C, trypsin A	Lateolabrax japonicus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
21000	-	x * 21000, trypsin A, SDS-PAGE, x * 21500, trypsin B, SDS-PAGE	Lateolabrax japonicus
21500	-	x * 21000, trypsin A, SDS-PAGE, x * 21500, trypsin B, SDS-PAGE	Lateolabrax japonicus

Organism

Organism	UniProt	Comment	Textmining
Lateolabrax japonicus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
native trypsin A and B, 210.5fold and 118.3fold, respectively, from hepatopancreas to homogeneity by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration	Lateolabrax japonicus

Source Tissue

Source Tissue	Comment	Organism	Textmining
hepatopancreas	-	Lateolabrax japonicus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
38.3	-	pH 8.0, 37°C, trypsin B, substrate benzyloxycarbonyl-L-phenylalaninyl-L-seryl-L-arginine-4-methylcoumaryl-7-amide	Lateolabrax japonicus
68.1	-	pH 8.0, 37°C, trypsin A, substrate benzyloxycarbonyl-L-phenylalaninyl-L-seryl-L-arginine-4-methylcoumaryl-7-amide	Lateolabrax japonicus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
benzyloxycarbonyl-L-glutaminyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide + H2O	-	Lateolabrax japonicus	benzyloxycarbonyl-L-glutaminyl-L-arginyl-L-arginine + 7-amino-4-methylcoumarin	-	?
benzyloxycarbonyl-L-glutamyl-L-lysyl-L-lysine-4-methylcoumaryl-7-amide + H2O	low activity	Lateolabrax japonicus	benzyloxycarbonyl-L-glutamyl-L-lysyl-L-lysine + 7-amino-4-methylcoumarin	-	?
benzyloxycarbonyl-L-leucyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide + H2O	-	Lateolabrax japonicus	benzyloxycarbonyl-L-leucyl-L-arginyl-L-arginine + 7-amino-4-methylcoumarin	-	?
benzyloxycarbonyl-L-leucyl-L-lysyl-L-arginine-4-methylcoumaryl-7-amide + H2O	-	Lateolabrax japonicus	benzyloxycarbonyl-L-leucyl-L-lysyl-L-arginine + 7-amino-4-methylcoumarin	-	?
benzyloxycarbonyl-L-phenylalaninyl-L-seryl-L-arginine-4-methylcoumaryl-7-amide + H2O	-	Lateolabrax japonicus	benzyloxycarbonyl-L-phenylalaninyl-L-seryl-L-arginine + 7-amino-4-methylcoumarin	-	?
benzyloxycarbonyl-L-valyl-L-leucyl-L-lysine-4-methylcoumaryl-7-amide + H2O	-	Lateolabrax japonicus	benzyloxycarbonyl-L-valyl-L-leucyl-L-lysine + 7-amino-4-methylcoumarin	-	?
benzyloxycarbonyl-L-valyl-L-prolyl-L-arginine-4-methylcoumaryl-7-amide + H2O	-	Lateolabrax japonicus	benzyloxycarbonyl-L-valyl-L-prolyl-L-arginine + 7-amino-4-methylcoumarin	-	?
additional information	Arg-4-methylcoumaryl-7-amide and Suc-Leu-Leu-Val-Lys-4-methylcoumaryl-7-amide are poor substrates, substrate specificities of trypsin A and B, overview	Lateolabrax japonicus	?	-	?
Z-Phe-Arg-4-methylcoumaryl-7-amide + H2O	low activity	Lateolabrax japonicus	Z-Phe-Arg + 7-amino-4-methylcoumarin	-	?

Subunits

Subunits	Comment	Organism
?	x * 21000, trypsin A, SDS-PAGE, x * 21500, trypsin B, SDS-PAGE	Lateolabrax japonicus

Synonyms

Synonyms	Comment	Organism
anionic trypsin	-	Lateolabrax japonicus
cationic trypsin	-	Lateolabrax japonicus
trypsin A	-	Lateolabrax japonicus
trypsin B	-	Lateolabrax japonicus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
40	-	trypsin A and trypsin B	Lateolabrax japonicus

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
20	40	optimal activity at 40°C, 40% of maximal activity at 20°C	Lateolabrax japonicus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
45	-	purified native trypsin A and trypsin B, stable up to	Lateolabrax japonicus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
3.2	-	benzyloxycarbonyl-L-valyl-L-leucyl-L-lysine-4-methylcoumaryl-7-amide	pH 8.0, 37°C, trypsin A	Lateolabrax japonicus
4.65	-	benzyloxycarbonyl-L-leucyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide	pH 8.0, 37°C, trypsin A	Lateolabrax japonicus
5.42	-	benzyloxycarbonyl-L-leucyl-L-lysyl-L-arginine-4-methylcoumaryl-7-amide	pH 8.0, 37°C, trypsin A	Lateolabrax japonicus
5.51	-	benzyloxycarbonyl-L-valyl-L-prolyl-L-arginine-4-methylcoumaryl-7-amide	pH 8.0, 37°C, trypsin A	Lateolabrax japonicus
8.07	-	benzyloxycarbonyl-L-valyl-L-leucyl-L-lysine-4-methylcoumaryl-7-amide	pH 8.0, 37°C, trypsin B	Lateolabrax japonicus
12.19	-	benzyloxycarbonyl-L-leucyl-L-lysyl-L-arginine-4-methylcoumaryl-7-amide	pH 8.0, 37°C, trypsin B	Lateolabrax japonicus
14.85	-	benzyloxycarbonyl-L-leucyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide	pH 8.0, 37°C, trypsin B	Lateolabrax japonicus
25.75	-	benzyloxycarbonyl-L-valyl-L-prolyl-L-arginine-4-methylcoumaryl-7-amide	pH 8.0, 37°C, trypsin B	Lateolabrax japonicus
41.14	-	benzyloxycarbonyl-L-glutaminyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide	pH 8.0, 37°C, trypsin B	Lateolabrax japonicus
42.99	-	benzyloxycarbonyl-L-glutaminyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide	pH 8.0, 37°C, trypsin A	Lateolabrax japonicus
67.79	-	benzyloxycarbonyl-L-phenylalaninyl-L-seryl-L-arginine-4-methylcoumaryl-7-amide	pH 8.0, 37°C, trypsin B	Lateolabrax japonicus
72.08	-	benzyloxycarbonyl-L-phenylalaninyl-L-seryl-L-arginine-4-methylcoumaryl-7-amide	pH 8.0, 37°C, trypsin A	Lateolabrax japonicus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9	-	trypsin A and trypsin B	Lateolabrax japonicus

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
7	11	purified native trypsin A and trypsin B, stable	Lateolabrax japonicus

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.00945	-	benzyloxycarbonyl-L-valyl-L-prolyl-L-arginine-4-methylcoumaryl-7-amide	pH 8.0, 37°C, trypsin A	Lateolabrax japonicus
0.0106	-	benzyloxycarbonyl-L-valyl-L-leucyl-L-lysine-4-methylcoumaryl-7-amide	pH 8.0, 37°C, trypsin A	Lateolabrax japonicus
0.0141	-	benzyloxycarbonyl-L-leucyl-L-lysyl-L-arginine-4-methylcoumaryl-7-amide	pH 8.0, 37°C, trypsin A	Lateolabrax japonicus
0.0149	-	benzyloxycarbonyl-L-leucyl-L-lysyl-L-arginine-4-methylcoumaryl-7-amide	pH 8.0, 37°C, trypsin B	Lateolabrax japonicus
0.0152	-	benzyloxycarbonyl-L-valyl-L-leucyl-L-lysine-4-methylcoumaryl-7-amide	pH 8.0, 37°C, trypsin B	Lateolabrax japonicus
0.0163	-	benzyloxycarbonyl-L-leucyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide	pH 8.0, 37°C, trypsin A	Lateolabrax japonicus
0.0417	-	benzyloxycarbonyl-L-leucyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide	pH 8.0, 37°C, trypsin B	Lateolabrax japonicus
0.0436	-	benzyloxycarbonyl-L-valyl-L-prolyl-L-arginine-4-methylcoumaryl-7-amide	pH 8.0, 37°C, trypsin B	Lateolabrax japonicus
0.0642	-	benzyloxycarbonyl-L-phenylalaninyl-L-seryl-L-arginine-4-methylcoumaryl-7-amide	pH 8.0, 37°C, trypsin A	Lateolabrax japonicus
0.0796	-	benzyloxycarbonyl-L-glutaminyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide	pH 8.0, 37°C, trypsin A	Lateolabrax japonicus
0.0926	-	benzyloxycarbonyl-L-phenylalaninyl-L-seryl-L-arginine-4-methylcoumaryl-7-amide	pH 8.0, 37°C, trypsin B	Lateolabrax japonicus
0.1084	-	benzyloxycarbonyl-L-glutaminyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide	pH 8.0, 37°C, trypsin B	Lateolabrax japonicus

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Release 2023.1 (January 2023)