Application | Comment | Organism |
---|---|---|
synthesis | attachment of O-carboxymethyl-poly-beta-cyclodextrin with molecular weight of 13000 Da to surface of enzyme. resulting neoglycoenzyme retains high proteolytic and esterolytic activity, optimum temperature is increased by 10°C, enzyme is more resistant to thermal inactivation and to denaturation by sodiumdodecyl sulfate | Bos taurus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | attachment of O-carboxymethyl-poly-beta-cyclodextrin with molecular weight of 13000 Da to surface of enzyme. Resulting neoglycoenzyme retains high proteolytic and esterolytic activity, optimum temperature is increased by 10°C, enzyme is more resistant to thermal inactivation and to denaturation by sodiumdodecyl sulfate | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
32 | - |
N-alpha-benzoyl-L-Arg ethyl ester | enzyme with O-carboxymethyl-poly-beta-cyclodextrin attached to surface, pH 8.0, 25°C | Bos taurus | |
35.5 | - |
N-alpha-benzoyl-L-Arg ethyl ester | wild-type, pH 8.0, 25°C | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
pancreas | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-alpha-benzoyl-L-Arg ethyl ester + H2O | - |
Bos taurus | N-alpha-benzoyl-L-Arg + ethanol | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
wild-type, half-life 58 min, enzyme with O-carboxymethyl-poly-beta-cyclodextrin attached to surface, half-life 187 min | Bos taurus |
50 | - |
wild-type, half-life 16 min, enzyme with O-carboxymethyl-poly-beta-cyclodextrin attached to surface, half-life 97 min. Presence of 1 M NaCl, wild-type, half-life 41 min, enzyme with O-carboxymethyl-poly-beta-cyclodextrin attached to surface, half-life 48 min. Presence of 0.1 mM adamantan-1-ol, wild-type, half-life 14 min, enzyme with O-carboxymethyl-poly-beta-cyclodextrin attached to surface, half-life 29 min | Bos taurus |
60 | - |
wild-type, half-life 6 min, enzyme with O-carboxymethyl-poly-beta-cyclodextrin attached to surface, half-life 28 min | Bos taurus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10.5 | - |
N-alpha-benzoyl-L-Arg ethyl ester | enzyme with O-carboxymethyl-poly-beta-cyclodextrin attached to surface, pH 8.0, 25°C | Bos taurus | |
12.3 | - |
N-alpha-benzoyl-L-Arg ethyl ester | wild-type, pH 8.0, 25°C | Bos taurus |