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Literature summary for 3.4.21.4 extracted from

  • Balti, R.; Barkia, A.; Bougatef, A.; Ktari, N.; Nasri, M.
    A heat-stable trypsin from the hepatopancreas of the cuttlefish (Sepia officinalis): Purification and characterisation (2009), Food Chem., 113, 146-154.
    View publication on PubMed
Search for more literature for 3.4.21.4

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ 27% inhibition at 5 mM Sepia officinalis
benzamidine 55% inhibition at 5 mM Sepia officinalis
Co2+ 48% inhibition at 5 mM Sepia officinalis
Cu2+ 68% inhibition at 5 mM Sepia officinalis
Mn2+ 34.5% inhibition at 5 mM Sepia officinalis
additional information no inhibition by pepstatin A, 2-mercaptoethanol and EDTA Sepia officinalis
NaCl 60% inhibition at 30% NaCl Sepia officinalis
PMSF 61% inhibition at 1 mM, complete inhibition at 5 mM Sepia officinalis
Soybean trypsin inhibitor SBT, complete inhibition at 1 mg/ml Sepia officinalis
Zn2+ 71% inhibition at 5 mM Sepia officinalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.064
-
 N-benzoyl-DL-arginine 4-nitroanilide pH 8.0, 25°C Sepia officinalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
 gel filtration Sepia officinalis

Organism

Organism UniProt Comment Textmining
Sepia officinalis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 26.7fold by ammonium sulfate fractionation, and two different alternating steps of gel filtration and anion-exchange chromatography Sepia officinalis

Source Tissue

Source Tissue Comment Organism Textmining
hepatopancreas
-
 Sepia officinalis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.12
-
 purified enzyme, pH 8.0, 25°C Sepia officinalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
casein + H2O
-
 Sepia officinalis ?
-
 ?
N-benzoyl-DL-arginine 4-nitroanilide + H2O
-
 Sepia officinalis N-benzoyl-DL-arginine + 4-nitroaniline
-
 ?
Nalpha-benzoyl-L-arginine ethyl ester + H2O
-
 Sepia officinalis Nalpha-benzoyl-L-arginine + ethanol
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 24000, SDS-PAGE Sepia officinalis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Sepia officinalis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 85 activity range, profile, overview Sepia officinalis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
10 60 1 h, purified enzyme, completely stable up to 40°C, 50% activity remaining at 60°C, inactive at 70°C Sepia officinalis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.32
-
 N-benzoyl-DL-arginine 4-nitroanilide pH 8.0, 25°C Sepia officinalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Sepia officinalis

pH Range

pH Minimum pH Maximum Comment Organism
5 10 activity range, profile, overview Sepia officinalis

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 10 1 h, purified enzyme, the enzyme maintains over 100% of its original activity between pH 7.0 and pH 10.0, and more than 60% of its activity at pH 11.0 and pH 12.0, unstable below pH 6.0, inactive at pH 4.0 Sepia officinalis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
36.3
-
 N-benzoyl-DL-arginine 4-nitroanilide pH 8.0, 25°C Sepia officinalis