Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.21.4 extracted from

  • Stosova, T.; Sebela, M.; Rehulka, P.; Sedo, O.; Havlis, J.; Zdrahal, Z.
    Evaluation of the possible proteomic application of trypsin from Streptomyces griseus (2008), Anal. Biochem., 376, 94-102.
    View publication on PubMed
Search for more literature for 3.4.21.4

General Stability

General Stability Organism
modification by biacetyl does not improve thermostability of SGT Streptomyces griseus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23110
-
 MALDI-TOF mass spectrometry Streptomyces griseus

Organism

Organism UniProt Comment Textmining
Streptomyces griseus P00775 precursor
-

Purification (Commentary)

Purification (Comment) Organism
SP-Sepharose Fast Flow column chromatography and Arg-Sepharose 4B Fast Flow column chromatography Streptomyces griseus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.34
-
 after 1fold purification, using N-benzoyl-DL-arginine-4-nitroanilide as a substrate Streptomyces griseus
9.42
-
 after 27.6fold purification, using N-benzoyl-DL-arginine-4-nitroanilide as a substrate Streptomyces griseus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-amylase + H2O
-
 Streptomyces griseus ?
-
 ?
Bovine serum albumin + H2O
-
 Streptomyces griseus ?
-
 ?
carbonic anhydrase + H2O
-
 Streptomyces griseus ?
-
 ?
Cytochrome c + H2O
-
 Streptomyces griseus ?
-
 ?
glucose oxidase + H2O
-
 Streptomyces griseus ?
-
 ?
glycogen phosphorylase b + H2O
-
 Streptomyces griseus ?
-
 ?
Hemoglobin + H2O
-
 Streptomyces griseus ?
-
 ?
Lysozyme + H2O
-
 Streptomyces griseus ?
-
 ?
Myoglobin + H2O
-
 Streptomyces griseus ?
-
 ?
N-benzoyl-DL-arginine-4-nitroanilide + H2O
-
 Streptomyces griseus N-benzoyl-DL-arginine + 4-nitroaniline
-
 ?
ovalbumin + H2O
-
 Streptomyces griseus ?
-
 ?
peroxidase + H2O
-
 Streptomyces griseus ?
-
 ?

Synonyms

Synonyms Comment Organism
SGT
-
 Streptomyces griseus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
42
-
 50% of the activity is retained on 30 min incubation at 42°C Streptomyces griseus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.8
-
 N-benzoyl-DL-arginine-4-nitroanilide
-
 Streptomyces griseus

pI Value

Organism Comment pI Value Maximum pI Value
Streptomyces griseus isoelectric focusing
-
 6.8