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Literature summary for 3.4.21.4 extracted from

  • Ladisch, M.R.; Kohlmann, K.L.
    Recombinant human insulin (1992), Biotechnol. Prog., 8, 469-478.
    View publication on PubMed

Application

Application Comment Organism
synthesis production of human insulin-threonine-ester, which can be simply converted into human insulin by hydrolysis with subsequent purification steps Sus scrofa
synthesis production of mono/di-arg insulin Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
pancreas
-
Sus scrofa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pre-Pro-Arg-insulin + H2O
-
Sus scrofa mono/di-Arg insulin + ?
-
?
Pro-Arg-insulin + H2O
-
Sus scrofa mono/di-Arg insulin + ?
-
?
pro-insulin + H2O
-
Sus scrofa human insulin-threonine-ester
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6
-
-
Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Sus scrofa
8.3
-
-
Sus scrofa