Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibited by 1-(L-trans-epoxysuccinyl-leucylamino)-4-guanidinobutane, N-ethylmaleimide, iodoacetic acid, N-tosyl-L-phenyl-alanine-chloromethyl ketone, and pepstatin | Thunnus albacares | |
N-tosyl-L-lysine-chloromethyl ketone | complete inhibition | Thunnus albacares | |
NaCl | activity decreases with increasing NaCl concentrations (0-30%) | Thunnus albacares | |
Soybean trypsin inhibitor | effective inhibitor | Thunnus albacares |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
N-alpha-p-tosyl-L-arginine methyl ester | trypsin B, at pH 8.0 and 30°C | Thunnus albacares | |
0.33 | - |
N-alpha-p-tosyl-L-arginine methyl ester | trypsin A, at pH 8.0 and 30°C | Thunnus albacares |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | stabilized in the presence of 2 mM Ca2+ | Thunnus albacares |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24000 | - |
SDS-PAGE and gel filtration | Thunnus albacares |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thunnus albacares | - |
yellowfin tuna | - |
Purification (Comment) | Organism |
---|---|
Sephacryl S-200 gel filtration, Sephadex G-50 gel filtration and DEAE-cellulose column chromatography | Thunnus albacares |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
spleen | - |
Thunnus albacares | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.11 | - |
crude extract, using N-alpha-p-tosyl-L-arginine methyl ester as a substrate, at pH 8.0 and 30°C | Thunnus albacares |
7.77 | - |
trypsin A after 70.6fold purification, using N-alpha-p-tosyl-L-arginine methyl ester as a substrate, at pH 8.0 and 30°C | Thunnus albacares |
10.07 | - |
trypsin B after 91.5fold purification, using N-alpha-p-tosyl-L-arginine methyl ester as a substrate, at pH 8.0 and 30°C | Thunnus albacares |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-alpha-p-tosyl-L-arginine methyl ester + H2O | - |
Thunnus albacares | N-alpha-p-tosyl-L-arginine + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 24000, SDS-PAGE and gel filtration | Thunnus albacares |
Synonyms | Comment | Organism |
---|---|---|
trypsin A | - |
Thunnus albacares |
trypsin B | - |
Thunnus albacares |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
trypsin A | Thunnus albacares |
65 | - |
trypsin B | Thunnus albacares |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
trypsin A and B are heat stable up to 50°C | Thunnus albacares |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
66.7 | - |
N-alpha-p-tosyl-L-arginine methyl ester | trypsin B, at pH 8.0 and 30°C | Thunnus albacares | |
80 | - |
N-alpha-p-tosyl-L-arginine methyl ester | trypsin A, at pH 8.0 and 30°C | Thunnus albacares |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Thunnus albacares |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | - |
Thunnus albacares |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | - |
trypsin B shows no activity, while trypsin A retains about 50% of activity at pH 4 | Thunnus albacares |
6 | 11 | - |
Thunnus albacares |