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Literature summary for 3.4.21.4 extracted from

  • Woodard, S.L.; Mayor, J.M.; Bailey, M.R.; Barker, D.K.; Love, R.T.; Lane, J.R.; Delaney, D.E.; McComas-Wagner, J.M.; Mallubhotla, H.D.; Hood, E.E.; Dangott, L.J.; Tichy, S.E.; Howard, J.A.
    Maize (Zea mays)-derived bovine trypsin: characterization of the first large-scale, commercial protein product from transgenic plants (2003), Biotechnol. Appl. Biochem., 38, 123-130.
    View publication on PubMed
Search for more literature for 3.4.21.4

Application

Application Comment Organism
medicine commercial-level production of trypsin in transgenic maize, the availability of this reagent should allow for the replacement of animal-derived trypsin in the processing of pharmaceutical proteins Bos taurus

Cloned(Commentary)

Cloned (Comment) Organism
commercial-level production of trypsin in transgenic maize, functionally equivalent to native bovine pancreatic trypsin Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7
-
 N-alpha-benzoyl-L-arginine 4-nitroanilide pH 8.3, 25°C, recombinant enzyme from Zea may Bos taurus
3.2
-
 N-alpha-benzoyl-L-arginine 4-nitroanilide pH 8.3, 25°C, native bovine trypsin Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23300
-
 MALDI-TOF mass spectrometry Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein recombinant bovine trypsin from maize has glycoforms Bos taurus

Purification (Commentary)

Purification (Comment) Organism
-
 Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-alpha-benzoyl-L-arginine 4-nitroanilide + H2O
-
 Bos taurus N-alpha-benzoyl-L-arginine + 4-nitroaniline
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.6
-
 recombinant enzyme from Zea may Bos taurus
8.7
-
 native bovine trypsin Bos taurus

pH Range

pH Minimum pH Maximum Comment Organism
6.9 10.2 pH 6.9: about 35% of maximal activity, pH 10.2: about 40-50% of maximal activity, native and recombinant enzyme Bos taurus