General Stability | Organism |
---|---|
Ca2+ stabilizes | Gadus macrocephalus |
Ca2+ stabilizes | Eleginus gracilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no or poor inhibition by E-64, N-ethylmaleimide, iodoacetic acid, TPCK, pepstatin A, and EDTA | Eleginus gracilis | |
additional information | no or poor inhibition by E-64, N-ethylmaleimide, iodoacetic acid, TPCK, pepstatin A, and EDTA | Gadus macrocephalus | |
Nalpha-tosyl-L-Lys-chloromethylketone | 79% inhibition at 5 mM | Eleginus gracilis | |
Nalpha-tosyl-L-Lys-chloromethylketone | 89% inhibition at 5 mM | Gadus macrocephalus | |
Soybean trypsin inhibitor | 84% inhibition at 1 mg/ml | Eleginus gracilis | |
Soybean trypsin inhibitor | 80% inhibition at 1 mg/ml | Gadus macrocephalus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | stabilizes | Gadus macrocephalus | |
Ca2+ | stabilizes | Eleginus gracilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24000 | - |
x * 24000, SDS-PAGE | Gadus macrocephalus |
24000 | - |
x * 24000, SDS-PAGE | Eleginus gracilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Eleginus gracilis | - |
- |
- |
Gadus macrocephalus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 28fold from pyloric ceca to homogeneity by gel filtration | Eleginus gracilis |
native enzyme 33fold from pyloric ceca to homogeneity by gel filtration | Gadus macrocephalus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cecum | - |
Gadus macrocephalus | - |
cecum | - |
Eleginus gracilis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
18 | - |
purified enzyme, pH 8.0, 50°C | Eleginus gracilis |
24 | - |
purified enzyme, pH 8.0, 50°C | Gadus macrocephalus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme from catayzes also the reverse reaction and synthesizes Nalpha-(tert-butoxycarbonyl)-L-alanyl-L-alanine-4-nitroanilide from Nalpha-(tert-butoxycarbonyl)-L-alanine-4-guanidinophenyl ester as acyl donor and L-alanine-p-nitroanilide (L-Ala-pNA) as acyl acceptor, overview | Gadus macrocephalus | ? | - |
? | |
Nalpha-(tert-butoxycarbonyl)-L-alanyl-L-alanine-4-nitroanilide + H2O | - |
Gadus macrocephalus | Nalpha-(tert-butoxycarbonyl)-L-alanyl-L-alanine + 4-nitroaniline | - |
? | |
Nalpha-p-tosyl-L-arginine methyl ester + H2O | - |
Gadus macrocephalus | ? | - |
? | |
Nalpha-p-tosyl-L-arginine methyl ester + H2O | - |
Eleginus gracilis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 24000, SDS-PAGE | Gadus macrocephalus |
? | x * 24000, SDS-PAGE | Eleginus gracilis |
Synonyms | Comment | Organism |
---|---|---|
EG-T | - |
Eleginus gracilis |
GM-T | - |
Gadus macrocephalus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Gadus macrocephalus |
50 | - |
- |
Eleginus gracilis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 70 | activity range | Gadus macrocephalus |
20 | 70 | activity range | Eleginus gracilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
purified enzyme, unstable above | Gadus macrocephalus |
30 | - |
purified enzyme, unstable above | Eleginus gracilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Gadus macrocephalus |
8 | - |
- |
Eleginus gracilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | high activity within this range | Gadus macrocephalus |
7 | 9 | high activity within this range | Eleginus gracilis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | purified enzyme, unstable below, completely stable at pH 6.0-10.0 for 30 min | Eleginus gracilis |
5 | 10 | purified enzyme, unstable below, completely stable at pH 7.0-10.0 for 30 min | Gadus macrocephalus |