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Literature summary for 3.4.21.4 extracted from
- Herstellung and einige Eigenschaften von immobilisiertem Trypsin des Flusskrebses Cambarus affinis say (1976), Hoppe-Seyler's Z. Physiol. Chem., 357, 629-639.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | enzyme immobilized on Sepharose has higher thermal stability than the wild-type enzyme, enzyme immobilized on porous glass loaded with isothiocyanate propyl groups has the same thermal stability as the wild-type enzyme, enzyme immobilized on DEAE-Sephadex has lower thermal stability than the wild-type enzyme | Cambarus affinis |
General Stability
| General Stability | Organism |
|---|---|
| enzyme immobilized on Sepharose has higher thermal stability than the wild-type enzyme, enzyme immobilized on porous glass loaded with isothiocyanate propyl groups has the same thermal stability as the wild-type enzyme, enzyme immobilized on DEAE-Sephadex has lower thermal stability than the wild-type enzyme | Cambarus affinis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.12 | - |
tosyl-Arg methyl ester | free enzyme | Cambarus affinis |  |
| 1.53 | - |
tosyl-Arg methyl ester | enzyme immobilized on Sepharose 4B-KT | Cambarus affinis | |
| 2.5 | - |
tosyl-Arg methyl ester | enzyme immobilized on porous glass loaded with isothiocyanate | Cambarus affinis | |
| 6.2 | - |
tosyl-Arg methyl ester | enzyme immobilized on Sepharose 4B-KT | Cambarus affinis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cambarus affinis | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| p-tosyl-L-Arg methyl ester + H2O | - |
Cambarus affinis | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
hydrolysis of tosyl-Arg methyl ester, enzyme immobilized on DEAE-Sephadex A-50 KT | Cambarus affinis |
| 8.5 | - |
hydrolysis of tosyl-Arg methyl ester, free enzyme | Cambarus affinis |
| 9 | - |
hydrolysis of tosyl-Arg methyl ester, enzyme immobilized on porous glass with isothiocyanate propyl groups | Cambarus affinis |
| 9.5 | - |
hydrolysis of tosyl-Arg methyl ester, enzyme immobilized on Sepharose 4B-KT | Cambarus affinis |
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