Information on EC 1.2.1.5 - aldehyde dehydrogenase [NAD(P)+]

for references in articles please use BRENDA:EC1.2.1.5
Word Map on EC 1.2.1.5
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)


The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea

top print hide
EC NUMBER
COMMENTARY hide
1.2.1.5
-
top print hide
RECOMMENDED NAME
GeneOntology No.
aldehyde dehydrogenase [NAD(P)+]
top print hide
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
an aldehyde + NAD(P)+ + H2O = a carboxylate + NAD(P)H + H+
show the reaction diagram
top print hide
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
redox reaction
-
-
-
-
reduction
top print hide
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
L-tryptophan degradation V (side chain pathway)
-
-
tryptophan metabolism
-
-
Glycolysis / Gluconeogenesis
-
-
Histidine metabolism
-
-
Tyrosine metabolism
-
-
Phenylalanine metabolism
-
-
beta-Alanine metabolism
-
-
Metabolism of xenobiotics by cytochrome P450
-
-
Drug metabolism - cytochrome P450
-
-
Metabolic pathways
-
-
Biosynthesis of secondary metabolites
-
-
Microbial metabolism in diverse environments
-
-
top print hide
SYSTEMATIC NAME
IUBMB Comments
aldehyde:NAD(P)+ oxidoreductase
-
top
top print hide
CAS REGISTRY NUMBER
COMMENTARY hide
9028-88-0
-
top print hide
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
narrow-leafed lupin
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
subspecies Zea mays mays
-
-
Manually annotated by BRENDA team
top print hide
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
top print hide
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,3-dihydroxyacetone + NAD+ + H2O
? + NADH + H+
show the reaction diagram
53.93% of the activity compared to acetaldehyde
-
-
?
1-formyl-6-methylpyrene + NADP+ + H2O
6-methylpyrene-1-carboxylic acid + NADPH + H+
show the reaction diagram
-
-
-
?
1-formyl-8-methylpyrene + NADP+ + H2O
8-methylpyrene-1-carboxylic acid + NADPH + H+
show the reaction diagram
-
-
-
?
1-formylpyrene + NADP+ + H2O
pyrene-1-carboxylic acid + NADPH + H+
show the reaction diagram
-
-
-
?
2,5-diyhydroxybenzaldehyde + NADP+ + H2O
2,5-dihydroxybenzoate + NADPH + H+
show the reaction diagram
-
-
-
-
?
2-bromobenzaldehyde + NAD+ + H2O
2-bromobenzoate + NADH + H+
show the reaction diagram
2-chlorobenzaldehyde + NAD+ + H2O
2-chlorobenzoate + NADH + H+
show the reaction diagram
2-fluorobenzaldehyde + NAD+ + H2O
2-fluorobenzoate + NADH + H+
show the reaction diagram
2-formylpyrene + NADP+ + H2O
pyrene-2-carboxylic acid + NADPH + H+
show the reaction diagram
-
-
-
?
2-methylbutyraldehyde + NAD+ + H2O
2-methylbutyrate + NADH + H+
show the reaction diagram
-
-
-
-
?
2-naphthaldehyde + NADH + H+
2-naphthyl alcohol + NAD+
show the reaction diagram
-
-
-
-
?
3,4-dihydroxymandelaldehyde + NAD+ + H2O
3,4-dihydroxyphenylmandelate + NADH + H+
show the reaction diagram
-
-
-
-
?
3,4-dihydroxyphenylacetaldehyde + NAD+
3,4-dihydroxyphenylacetate + NADH
show the reaction diagram
-
-
-
-
?
3,4-dihydroxyphenylacetaldehyde + NAD+ + H2O
3,4-dihydroxyphenylacetate + NADH + H+
show the reaction diagram
-
-
-
?
3,4-dihydroxyphenylglycolaldehyde + NAD+ + H2O
3,4-dihydroxyphenylglycolate + NADH + H+
show the reaction diagram
-
-
-
?
3-chloroallylaldehyde + NAD+ + H2O
? + NADH + H+
show the reaction diagram
-
-
-
-
?
3-chloroallylaldehyde + NADP+ + H2O
? + NADPH + H+
show the reaction diagram
-
-
-
-
?
3-hydroxybenzaldehyde + NADP+ + H2O
3-hydroxybenzoate + NADPH + H+
show the reaction diagram
-
-
-
-
?
3-hydroxypropionaldehyde + NAD+ + H2O
3-hydroxypropanoate + NADH + 2 H+
show the reaction diagram
3-nitrobenzaldehyde + NAD+ + H2O
3-nitrobenzoate + NADH + H+
show the reaction diagram
32.98% of the activity compared to acetaldehyde
-
-
?
4-bromobenzaldehyde + NAD+ + H2O
4-bromobenzoate + NADH + H+
show the reaction diagram
-
-
-
-
?
4-carboxybenzaldehyde + NADP+ + H2O
4-carboxybenzoate + NADPH + H+
show the reaction diagram
-
-
-
-
?
4-chlorobenzaldehyde + NADP+ + H2O
4-chlorobenzoate + NADPH + H+
show the reaction diagram
-
-
-
-
?
4-fluorobenzaldehyde + NAD+ + H2O
4-fluorobenzoate + NADH + H+
show the reaction diagram
-
-
-
-
?
4-formylpyrene + NADP+ + H2O
pyrene-4-carboxylic acid + NADPH + H+
show the reaction diagram
-
-
-
?
4-hydroxy-2-nonenal + NAD(P)+ + H2O
? + NAD(P)H + H+
show the reaction diagram
-
-
-
-
?
4-nitrobenzaldehyde + NAD+ + H2O
4-nitrobenzoate + NADH + H+
show the reaction diagram
-
-
-
-
?
4-pyridinecarboxaldehyde + NAD+ + H2O
4-pyridinecarboxylate + NADH + H+
show the reaction diagram
-
-
-
-
?
5-hydroxyindole-3-acetyldehyde + NAD+ + H2O
5-hydroxyindole-3-acetate + NADH + H+
show the reaction diagram
-
-
-
-
?
6-methoxy-2-naphthaldehyde + NADH + H+
6-methoxy-2-naphthyl alcohol + NAD+
show the reaction diagram
-
-
-
-
?
acetaldehyde + 3-pyridine aldehyde adenine dinucleotide phosphate
acetate + ?
show the reaction diagram
-
-
-
-
?
acetaldehyde + acetylpyridine adenine dinucleotide
acetate + ?
show the reaction diagram
-
-
-
-
?
acetaldehyde + deamino-NAD+ + H2O
acetate + deamino-NADH + H+
show the reaction diagram
-
-
-
-
?
acetaldehyde + NAD(P)+ + H2O
acetate + NAD(P)H + H+
show the reaction diagram
acetaldehyde + NAD+
acetate + NADH + H+
show the reaction diagram
-
-
-
-
?
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
acetaldehyde + NADP+
acetate + NADPH + H+
show the reaction diagram
-
-
-
-
?
acetaldehyde + NADP+ + H2O
acetate + NADPH
show the reaction diagram
-
-
-
?
acetaldehyde + NADP+ + H2O
acetate + NADPH + H+
show the reaction diagram
acetaldehyde + thio-NAD+ + H2O
acetate + thio-NADH + H+
show the reaction diagram
-
-
-
-
?
acetaldehyde + thio-NADP+
acetate + ?
show the reaction diagram
-
-
-
-
?
aldophosphamide + NAD+
N,N-bis-(2-chloroethyl)-diaminophosphoric-(2-carboxyethylester) + ?
show the reaction diagram
-
-
-
-
?
all-trans-retinal + NAD+ + H2O
all-trans-retinoic acid + NADH + H+
show the reaction diagram
all-trans-retinal + NADP+ + H2O
all-trans-retinol + NADPH + H+
show the reaction diagram
an aldehyde + NAD(P)+ + H2O
an acid + NAD(P)H + H+
show the reaction diagram
an aldehyde + NAD+ + H2O
a carboxylate + NADH + H+
show the reaction diagram
an aldehyde + NADP+ + H2O
a carboxylate + NADPH + H+
show the reaction diagram
benzaldehyde + NAD+ + H2O
benzoate + NADH + H+
show the reaction diagram
benzaldehyde + NADH + H+
benzyl alcohol + NAD+
show the reaction diagram
-
-
-
-
?
benzaldehyde + NADP+
benzoate + NADPH
show the reaction diagram
benzaldehyde + NADP+ + H2O
benzoate + NADPH
show the reaction diagram
benzaldehyde + NADP+ + H2O
benzoate + NADPH + H+
show the reaction diagram
butyraldehyde + NAD(P)+ + H2O
butanoate + NAD(P)H + H+
show the reaction diagram
butyraldehyde + NAD+
butyrate + NADH + H+
show the reaction diagram
-
-
-
-
?
butyraldehyde + NAD+ + H2O
butyrate + NADH + H+
show the reaction diagram
butyraldehyde + NADP+
butyrate + NADPH + H+
show the reaction diagram
-
-
-
-
?
chloroacetaldehyde + NAD+ + H2O
chloroacetate + NADH + H+
show the reaction diagram
cinnamaldehyde + NAD+ + H2O
cinnamate + NADH + H+
show the reaction diagram
-
-
-
-
?
cinnamaldehyde + NADH + H+
cinnamyl alcohol + NAD+
show the reaction diagram
-
-
-
-
?
D-glyceraldehyde + NAD+ + H2O
D-glycerate 3-phosphate + NADH + H+
show the reaction diagram
50.25% of the activity compared to acetaldehyde. kcat/KM for NAD+ with D-glyceraldehyde as cosubstrate is 3.8fold higher compared to NADP+
-
-
?
D-glyceraldehyde + NADP+ + H2O
D-glycerate + NADPH
show the reaction diagram
50.25% of the activity compared to acetaldehyde
-
-
?
D-glyceraldehyde 3-phosphate + NAD+ + H2O
D-glycerate 3-phosphate + NADH + H+
show the reaction diagram
52.7% of the activity compared to acetaldehyde
-
-
?
DL-glyceraldehyde + NAD(P)+ + H2O
glycerate + NAD(P)H + H+
show the reaction diagram
-
14% of activity with benzaldehyde
-
-
ir
dodecanal + NADP+ + H2O
dodecanoate + NADPH + H+
show the reaction diagram
-
-
-
-
?
formaldehyde + NAD(P)+ + H2O
formate + NAD(P)H + H+
show the reaction diagram
formaldehyde + NAD+ + H2O
formate + NADH + H+
show the reaction diagram
formaldehyde + NADP+ + H2O
formate + NADPH + H+
show the reaction diagram
furfuryl aldehyde + NAD+ + H2O
2-furancarboxylate + NADH + H+
show the reaction diagram
glutaraldehyde + NAD+
glutarate + NADH
show the reaction diagram
-
-
-
-
?
glyceraldehyde + NAD(P)+ + H2O
glycerate + NAD(P)H + H+
show the reaction diagram
Q8KZT4;
-
-
-
ir
glyceraldehyde + NAD+ + H2O
glycerate + NADH + H+
show the reaction diagram
-
-
-
-
?
glycolaldehyde + NAD+
glycolate + NADH
show the reaction diagram
glycolaldehyde + NAD+ + H2O
glycolate + NADH + H+
show the reaction diagram
75.43% of the activity compared to acetaldehyde
-
-
?
glycolaldehyde + NADP+ + H2O
glycolate + NADPH + H+
show the reaction diagram
heptanal + NADP+ + H2O
heptanoate + NADPH + H+
show the reaction diagram
-
-
-
?
heptylaldehyde + NAD(P)+ + H2O
heptanoate + NAD(P)H + H+
show the reaction diagram
hexanal + NAD+ + H2O
hexanoate + NADH + H+
show the reaction diagram
hexylaldehyde + NAD(P)+ + H2O
hexanoate + NAD(P)H + H+
show the reaction diagram
indole-3-acetaldehyde + NAD+ + H2O
indole-3-acetate + NADH + H+
show the reaction diagram
-
-
-
?
isobutanal + NAD+ + H2O
isobutyrate + NADH + H+
show the reaction diagram
isobutyraldehyde + NAD(P)+ + H2O
isobutanoate + NAD(P)H + H+
show the reaction diagram
isopentanal + NAD+ + H2O
isopentanoate + NADH + H+
show the reaction diagram
-
-
-
-
?
isovaleraldehyde + NAD(P)+ + H2O
isovalerate + NAD(P)H
show the reaction diagram
isovaleraldehyde + NAD+
isovalerate + NADH + H+
show the reaction diagram
-
-
-
-
?
isovaleraldehyde + NADP+
isovalerate + NADPH + H+
show the reaction diagram
isovaleraldehyde + NADP+ + H2O
isovalerate + NADPH + H+
show the reaction diagram
-
-
-
-
m-bromobenzaldehyde + NAD(P)+ + H2O
m-bromobenzoate + NAD(P)H + H+
show the reaction diagram
m-chlorobenzaldehyde + NAD(P)+ + H2O
m-chlorobenzoate + NAD(P)H + H+
show the reaction diagram
m-fluorobenzaldehyde + NAD(P)+ + H2O
m-fluorobenzoate + NAD(P)H + H+
show the reaction diagram
mafosfamide + NADP+ + H2O
?
show the reaction diagram
-
-
-
-
?
malondialdehyde + NAD(P)+ + H2O
? + NAD(P)H + H+
show the reaction diagram
-
-
-
-
?
malondialdehyde + NAD+
? + NADH
show the reaction diagram
malonic semialdehyde + NAD+
?
show the reaction diagram
-
-
-
-
?
n-butanal + NAD+ + H2O
butanoate + NADH + H+
show the reaction diagram
n-butanal + NADP+ + H2O
n-butanoate + NADPH + H+
show the reaction diagram
n-decanal + NADP+ + H2O
n-decanoate + NADPH + H+
show the reaction diagram
n-dodecanal + NADP+ + H2O
n-dodecanoate + NADPH + H+
show the reaction diagram
-
-
-
-
?
n-hexanal + NADP+ + H2O
n-hexanoate + NADPH + H+
show the reaction diagram
-
-
-
-
?
n-nonanal + NADP+ + H2O
n-nonanoate + NADPH + H+
show the reaction diagram
-
-
-
-
?
n-octanal + NADP+ + H2O
n-octanoate + NADPH + H+
show the reaction diagram
-
-
-
-
?
n-pentanal + NAD+ + H2O
n-pentanoate + NADH + H+
show the reaction diagram
n-pentanal + NADP+ + H2O
n-pentanoate + NADPH + H+
show the reaction diagram
-
-
-
-
?
n-propanal + NADP+
propionate + NADPH + H+
show the reaction diagram
-
-
-
-
?
n-propanal + NADP+ + H2O
n-propanoate + NADPH + H+
show the reaction diagram
-
-
-
-
?
n-tetradecanal + NADP+ + H2O
n-tetradecanoate + NADPH + H+
show the reaction diagram
-
-
-
-
?
n-tridecanal + NADP+ + H2O
n-tridecanoate + NADPH + H+
show the reaction diagram
-
-
-
-
?
n-undecanal + NADP+ + H2O
n-undecanoate + NADPH + H+
show the reaction diagram
-
-
-
-
?
nonanal + NADP+ + H2O
nonanoate + NADPH + H+
show the reaction diagram
-
-
-
-
?
o-fluorobenzaldehyde + NAD(P)+ + H2O
o-fluorobenzoate + NAD(P)H + H+
show the reaction diagram
o-methoxybenzaldehyde + NAD(P)+ + H2O
o-methoxybenzoate + NAD(P)H + H+
show the reaction diagram
octanal + NAD+ + H2O
octanoate + NADH + H+
show the reaction diagram
octylaldehyde + NAD(P)+ + H2O
octanoate + NAD(P)H + H+
show the reaction diagram
p-bromobenzaldehyde + NAD(P)+ + H2O
p-bromobenzoate + NAD(P)H + H+
show the reaction diagram
p-chlorobenzaldehyde + NAD(P)+ + H2O
p-chlorobenzoate + NAD(P)H + H+
show the reaction diagram
p-fluorobenzaldehyde + NAD(P)+ + H2O
p-fluorobenzoate + NAD(P)H + H+
show the reaction diagram
p-hydroxybenzaldehyde + NADP+ + H2O
p-hydroxybenzoic acid + NADPH + H+
show the reaction diagram
-
-
-
-
?
p-isopropylbenzaldehyde + NAD(P)+ + H2O
p-isopropylbenzoate + NAD(P)H + H+
show the reaction diagram
p-nitrobenzaldehyde + NADP+ + H2O
p-nitrobenzoate + NADPH + H+
show the reaction diagram
-
-
-
-
?
phenylacetaldehyde + NAD+ + H2O
phenylacetate + NADH + H+
show the reaction diagram
-
-
-
-
?
phenylacetaldehyde + NADP+ + H2O
phenylacetate + NADPH + H+
show the reaction diagram
-
-
-
-
?
polyethylene glycol 1000-aldehyde + NADP+
? + NADPH
show the reaction diagram
-
-
-
-
?
polyethylene glycol 200-aldehyde + NADP+
? + NADPH
show the reaction diagram
-
-
-
-
?
polyethylene glycol 400-aldehyde + NADP+
? + NADPH
show the reaction diagram
-
-
-
-
?
polyethylene glycol 4000-aldehyde + NADP+
? + NADPH
show the reaction diagram
-
highest activtiy compared to other polyethylene glycolaldehydes
-
-
?
polyethylene glycol 6000-aldehyde + NADP+
? + NADPH
show the reaction diagram
-
-
-
-
?
propanal + 1,N6-ethenoadenine dinucleotide
propionate + ?
show the reaction diagram
-
isoenzyme E1 and E2
-
-
?
propanal + 3-acetylpyridine adenine dinucleotide
propionate + ?
show the reaction diagram
-
isoenzyme E1 and E2
-
-
?
propanal + 3-pyridine adenine dinucleotide
propionate + ?
show the reaction diagram
-
isoenzyme E1 and E2
-
-
?
propanal + 3-thionicotinamide adenine dinucleotide
propionate + ?
show the reaction diagram
propanal + beta-NADP+ + H2O
propionate + beta-NADPH + H+
show the reaction diagram
-
isoenzyme E1 and E2
-
-
?
propanal + N-guanine dinucleotide
propionate + ?
show the reaction diagram
-
isoenzyme E1 and E2
-
-
?
propanal + N-hypoxanthine dinucleotide
propionate + ?
show the reaction diagram
-
isoenzyme E1 and E2
-
-
?
propanal + NAD+ + H2O
propionate + NADH + H+
show the reaction diagram
propionaldehyde + NAD(P)+ + H2O
propionate + NAD(P)H
show the reaction diagram
propionaldehyde + NAD+ + H2O
propionate + NADH + H+
show the reaction diagram
propionaldehyde + NADP+ + H2O
propionate + NADPH + H+
show the reaction diagram
retinal + NAD+ + H2O
retinoic acid + NADH + H+
show the reaction diagram
preferred substrate of isozyme ALDH1A3
-
-
?
succinic semialdehyde + NAD+
? + NADH
show the reaction diagram
-
-
-
-
?
trans-2-hexenal + NAD+
trans-2-hexenoate + NADH
show the reaction diagram
valeraldehyde + NAD(P)+ + H2O
valerate + NAD(P)H + H+
show the reaction diagram
valeraldehyde + NAD+
valerate + NADH + H+
show the reaction diagram
-
-
-
-
?
valeraldehyde + NADP+
valerate + NADPH + H+
show the reaction diagram
valeraldehyde + NADP+ + H2O
valerate + NADPH + H+
show the reaction diagram
-
-
-
?
additional information
?
-
top print hide
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3-chloroallylaldehyde + NAD+ + H2O
? + NADH + H+
show the reaction diagram
-
-
-
-
?
3-chloroallylaldehyde + NADP+ + H2O
? + NADPH + H+
show the reaction diagram
-
-
-
-
?
4-hydroxy-2-nonenal + NAD(P)+ + H2O
? + NAD(P)H + H+
show the reaction diagram
-
-
-
-
?
acetaldehyde + NAD(P)+ + H2O
acetate + NAD(P)H + H+
show the reaction diagram
-
-
-
-
ir
an aldehyde + NAD(P)+ + H2O
an acid + NAD(P)H + H+
show the reaction diagram
an aldehyde + NAD+ + H2O
a carboxylate + NADH + H+
show the reaction diagram
an aldehyde + NADP+ + H2O
a carboxylate + NADPH + H+
show the reaction diagram
chloroacetaldehyde + NAD+ + H2O
chloroacetate + NADH + H+
show the reaction diagram
-
-
-
-
?
malondialdehyde + NAD(P)+ + H2O
? + NAD(P)H + H+
show the reaction diagram
-
-
-
-
?
propionaldehyde + NAD+ + H2O
propionate + NADH + H+
show the reaction diagram
-
-
-
-
?
additional information
?
-
top print hide
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADH
-
-
top print hide
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
; stimulating
CaCl2
-
30 mM, 3.8fold stimulation of activity of ALDH1
KCl
-
120 mM: 40fold increase of activity of ALDH5 with NAD+. 16 mM: 20fold increase of activity of enzyme ALDH5 with NADP+. No stimulation of the activity of enzyme form ALDH1
Li+
-
500 mM, 107% of the activity compared to activity without metal ions
MnCl2
-
2 mM, 2.1fold stimulation of activity of ALDH1
Na+
-
500 mM, 1.2fold activation
additional information
-
the enzyme does not require metal ions for its enzymatic reaction
top print hide
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,2-Cyclohexanedione
-
33% residual activity at 2.5 mM
2,4-Dinitro-1-fluorobenzene
-
11% residual activity at 2.5 mM
2-mercaptoethanol
-
10% (v/v), 55% inhibition
3-thionicotinamide adenine dinucleotide
-
strong inhibition versus beta-NAD+, isoenzyme E2; substrate inhibition, isoenzyme E1
4-hydroxy-2-nonenal
-
-
acetaldehyde
-
strong substrate inhibition
acetone
-
10% (v/v), 21% inhibition
benomyl
potent irreversible inhibitor of ALDH. Benomyl increases superoxide production as much as nitroglycerin
Benzoate
-
non-competitive with respect to either NAD+ or benzaldehyde
beta-NADP+
-
substrate inhibition, isoenzyme E1
Cd2+
-
100% inhibition at 1 mM; 1 mM, 100% inhibition
Chloral hydrate
Co2+
-
89% residual activity at 1 mM
diethyl dicarbonate
-
2% residual activity at 2.5 mM
dimethyl ampal thiolester
-
complete inhibition of isozyme ALDH3 at 0.0075 mM
Disulfiram
DMSO
-
10% (v/v), 32% inhibition
EDTA
-
50 mM, 22% inhibition
ethanediol
-
10% (v/v), 60% inhibition
ethyl acetimidate
-
71% residual activity at 250 mM
Fe2+
-
88% residual activity at 1 mM
guanidine hydrochloride
-
10% (v/v), 42% inhibition
H2O2
-
the loss of activity induced by H2O2 can be restored by reducing agents, although a decrease of the restored activity is observed upon addition of 10 mM dithiothreitol
methyl ampal thiolester
-
78% inhibition of isozyme ALDH3 at 0.0075 mM
Mg2+
-
89% residual activity at 1 mM
Mn2+
-
89% residual activity at 1 mM
N-Acetylimidazole
-
75% residual activity at 2.5 mM
N-bromosuccinimide
-
66% residual activity at 0.5 mM
nitroglycerin
p-chloromercuribenzoate
-
50 microM, 80% inhibition
p-hydroxymercuribenzoate
p-mercuribenzoate
-
80% inhibition at 0.05 mM
Pb2+
-
100% inhibition at 1 mM; 1 mM, 100% inhibition
PEG-400
-
10% (v/v), 16% inhibition
pentanal
-
extremely strong substrate inhibition
phenylmethylsulfonyl fluoride
-
73% residual activity at 2.5 mM
PMSF
-
50 mM, no loss of activity
pyrazole
-
100% inhibition at 1 mM; 1 mM, 100% inhibition
trans-4-(N,N-dimethylamino)-cinnamaldehyde
-
1 mM, 58% inhibition; 58% inhibition at 1 mM
Urea
-
500 mM, 44% inhibition
Woodward's reagent K
-
71% residual activity at 50 mM
ZnCl2
-
50% inhibition at 0.035 mM, enzyme form ALDH1
additional information
-
not inhibited by EDTA
-
top print hide
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
-
246% activity at 2 mM
dithiothreitol
-
276% activity at 2 mM
ethanol
-
0.05 mM, 1.8fold stimulation
H2O2
-
0.015 mM, 1.2fold stimulation
NH4+
-
500 mM, 1.31fold activation
Sodium dodecyl sulfate
-
0.01 mM, 1.1fold stimulation
top print hide
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.096
1,N6-ethenoadenine dinucleotide
-
isoenzyme E2
0.0016
1-formyl-6-methylpyrene
-
ALDH3A1, at pH 7.5 and 37°C
0.004
1-formyl-8-methylpyrene
-
ALDH3A1,at pH 7.5 and 37°C
0.004
1-formylpyrene
-
ALDH3A1, at pH 7.5 and 37°C
0.012 - 0.379
2-Bromobenzaldehyde
0.006 - 0.063
2-Chlorobenzaldehyde
0.008 - 0.822
2-Fluorobenzaldehyde
0.011
2-formylpyrene
-
ALDH3A1, at pH 7.5 and 37°C
0.0007
2-naphthaldehyde
-
pH 7.5, 25°C
0.39
3,4-dihydroxyphenyl acetaldehyde
-
reaction with NAD+
0.61
3,4-dihydroxyphenyl benzaldehyde
-
reaction with NAD+
0.0004 - 0.015
3,4-dihydroxyphenylacetaldehyde
0.0132 - 0.62
3-acetylpyridine adenine dinucleotide
0.0338 - 0.504
3-pyridinealdehyde adenine dinucleotide
0.0151
3-thionicotinamide adenine dinucleotide
-
isoenzyme E1
0.039 - 0.617
4-bromobenzaldehyde
0.031 - 1.159
4-Chlorobenzaldehyde
0.012 - 2.057
4-Fluorobenzaldehyde
0.00078
4-formylpyrene
-
ALDH3A1, at pH 7.5 and 37°C
0.03
4-hydroxynonenal
-
-
0.081 - 1.103
4-iodobenzaldehyde
0.091 - 0.19
4-Pyridinecarboxaldehyde
0.15
5-hydroxyindole-3-acetaldehyde
-
reaction with NAD+
0.0033
6-methoxy-2-naphthaldehyde
-
pH 7.5, 25°C
0.0002 - 393.4
acetaldehyde
0.64
aldophosphamide
-
reaction with NAD+
0.007
all-trans-retinal
-
with NAD+ as cosubstrate, at pH 7.0 and 37°C
0.013 - 4.15
benzaldehyde
0.0047
beta-NAD+
-
-
7.68
beta-NADP+
-
isoenzyme E1
0.094
betaNAD+
-
isoenzyme E2
2.95 - 4.93
Butyraldehyde
0.01 - 0.46
Chloroacetaldehyde
0.0053
cinnamaldehyde
-
pH 7.5, 25°C
8.51
D-glyceraldehyde
-
pH 8, 80°C
6.12
D-glyceraldehyde 3-phosphate
-
pH 8, 80°C
0.27 - 1.9
formaldehyde
0.05 - 0.13
glycolaldehyde
0.06 - 0.08
Hexanal
0.5
Indole-3-acetaldehyde
-
reaction with NAD+
0.016
Isobutyraldehyde
Q8KZT4;
30°C, pH 10.0
0.0005
isopentanal
-
isoenzyme F1
1.66 - 10.78
Isovaleraldehyde
0.0113
N-1,N6-ethenoadenine dinucleotide
-
isoenzyme E1
0.137
n-dodecanal
-
50 mM potassium phosphate buffer pH8.0, 1 mM CaCl2, 36°C
1.33
N-guanine dinucleotide
-
isoenzyme E1
0.121 - 1.36
N-hypoxanthine dinucleotide
0.003 - 35
NAD+
0.01 - 260
NADP+
0.067 - 0.113
octanal
0.0001
pentanal
-
isoenzyme F1
0.0003 - 0.004
phenylacetaldehyde
0.00039 - 19.06
propanal
0.46 - 12
propionaldehyde
0.1 - 0.12
trans-2-hexenal
0.12 - 0.34
Valeraldehyde
additional information
additional information
-
-
-
top print hide
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
26.1
4-hydroxynonenal
-
-
0.183 - 35.9
acetaldehyde
0.005
all-trans-retinal
-
with NAD+ as cosubstrate, at pH 7.0 and 37°C
0.217 - 51
benzaldehyde
11.5 - 32.8
Butyraldehyde
0.47 - 7.7
Chloroacetaldehyde
0.92
D-glyceraldehyde
-
pH 8, 80°C
0.83
D-glyceraldehyde 3-phosphate
-
pH 8, 80°C
34
Hexanal
0.91 - 3.58
Isovaleraldehyde
0.0007 - 45.3
NAD+
3.71 - 38.8
NADP+
13.36 - 49.38
propionaldehyde
35.1 - 53
trans-2-hexenal
19.41 - 30.99
Valeraldehyde
additional information
additional information
-
-
-
top print hide
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.003 - 1.85
acetaldehyde
0.67
all-trans-retinal
-
with NAD+ as cosubstrate, at pH 7.0 and 37°C
1.08
benzaldehyde
-
at pH 7.0 and 37°C
2.39 - 11.89
Butyraldehyde
5.5 - 200
Chloroacetaldehyde
0.11
D-glyceraldehyde
-
pH 8, 80°C
0.14
D-glyceraldehyde 3-phosphate
-
pH 8, 80°C
0.09 - 0.36
Isovaleraldehyde
0.16 - 158.3
NAD+
0.974 - 0.98
NADP+
20.67 - 107.5
propionaldehyde
56.66 - 257
Valeraldehyde
top print hide
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
6.9
acetaldehyde
-
-
0.19 - 0.2
Chloral hydrate
0.012 - 0.069
Disulfiram
top print hide
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.07
-
cell free extract
0.212
-
activity with NADP+
0.35
-
isoenzyme F1
0.378
-
activity with NAD+
0.42
-
activity in isolated mitochondria
0.49
-
after the Ni-NTA purification step
1
-
isoenzyme F2
1.48
-
recombinant enzyme, using 3-hydroxypropionaldehyde and NAD+ as substrates, at pH 8.0 and 37°C
5.51
-
-
14.1
Q8KZT4;
-
16.67
-
enzyme from breast adenocarcinoma cell line
18.92
-
-
32.95
-
enzyme from stomach mucosa
110.5
-
liver enzyme
top print hide
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8 - 8.5
-
benzaldehyde oxidation, enzyme from breast adenocarcinoma cell line
8 - 9
-
benzaldehyde oxidation, enzyme from stomach mucosa
9 - 9.5
-
-
9.3
-
reaction with propanal and NAD+ or NADP+
10 - 10.5
Q8KZT4;
-
10
-
activity with NAD+, broad plateau from pH 5 to 8 followed by a sharp increase in activity to pH 10
top print hide
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 9
-
pH 6.0: about 60% of maximal activity, pH 9.0: about 90% of maximal activity
6.2 - 6.7
-
pH 6.2: about 80% of maximal activity, pH 6.7: about 80% of maximal activity
top print hide
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
36
-
; activity assay
55 - 60
Q8KZT4;
-
top print hide
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
70 - 90
-
70°C: about 50% of maximal activity, 90°C: about 90% of maximal activity
top print hide
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
exhibiting oxazaphosphorine-specific acquired resistance, enzyme form ALDH3
Manually annotated by BRENDA team
-
isoform ALDH3A1
Manually annotated by BRENDA team
isozyme Aldh1a3 mRNA expression is highest in placenta
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
additional information
addition of ALDH to RASM cell homogenates significantly decreases NO consumption
Manually annotated by BRENDA team
top print hide
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
isoform ALDH3I1
Manually annotated by BRENDA team
-
isoforms ALDH3F1 and ALDH3H1
Manually annotated by BRENDA team
-
enzyme III
-
Manually annotated by BRENDA team
-
isoforms ALDH3I1 and ALDH3H1
Manually annotated by BRENDA team
-
isoform ALDH3H1
Manually annotated by BRENDA team
top print hide
PDB
SCOP
CATH
UNIPROT
ORGANISM
Homo sapiens;
P11883
Rattus norvegicus;