Information on EC 1.2.1.5 - aldehyde dehydrogenase [NAD(P)+]

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea

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EC NUMBER
COMMENTARY hide
1.2.1.5
-
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RECOMMENDED NAME
GeneOntology No.
aldehyde dehydrogenase [NAD(P)+]
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
an aldehyde + NAD(P)+ + H2O = a carboxylate + NAD(P)H + H+
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
redox reaction
-
-
-
-
reduction
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
L-tryptophan degradation V (side chain pathway)
-
-
tryptophan metabolism
-
-
Glycolysis / Gluconeogenesis
-
-
Histidine metabolism
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-
Tyrosine metabolism
-
-
Phenylalanine metabolism
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-
beta-Alanine metabolism
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Metabolism of xenobiotics by cytochrome P450
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Drug metabolism - cytochrome P450
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Metabolic pathways
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Biosynthesis of secondary metabolites
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Microbial metabolism in diverse environments
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-
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SYSTEMATIC NAME
IUBMB Comments
aldehyde:NAD(P)+ oxidoreductase
-
top
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CAS REGISTRY NUMBER
COMMENTARY hide
9028-88-0
-
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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-
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Manually annotated by BRENDA team
narrow-leafed lupin
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Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
subspecies Zea mays mays
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-
Manually annotated by BRENDA team
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,3-dihydroxyacetone + NAD+ + H2O
? + NADH + H+
show the reaction diagram
53.93% of the activity compared to acetaldehyde
-
-
?
1-formyl-6-methylpyrene + NADP+ + H2O
6-methylpyrene-1-carboxylic acid + NADPH + H+
show the reaction diagram
-
-
-
?
1-formyl-8-methylpyrene + NADP+ + H2O
8-methylpyrene-1-carboxylic acid + NADPH + H+
show the reaction diagram
-
-
-
?
1-formylpyrene + NADP+ + H2O
pyrene-1-carboxylic acid + NADPH + H+
show the reaction diagram
-
-
-
?
2,5-diyhydroxybenzaldehyde + NADP+ + H2O
2,5-dihydroxybenzoate + NADPH + H+
show the reaction diagram
-
-
-
-
?
2-bromobenzaldehyde + NAD+ + H2O
2-bromobenzoate + NADH + H+
show the reaction diagram
2-chlorobenzaldehyde + NAD+ + H2O
2-chlorobenzoate + NADH + H+
show the reaction diagram
2-fluorobenzaldehyde + NAD+ + H2O
2-fluorobenzoate + NADH + H+
show the reaction diagram
2-formylpyrene + NADP+ + H2O
pyrene-2-carboxylic acid + NADPH + H+
show the reaction diagram
-
-
-
?
2-methylbutyraldehyde + NAD+ + H2O
2-methylbutyrate + NADH + H+
show the reaction diagram
-
-
-
-
?
2-naphthaldehyde + NADH + H+
2-naphthyl alcohol + NAD+
show the reaction diagram
-
-
-
-
?
3,4-dihydroxymandelaldehyde + NAD+ + H2O
3,4-dihydroxyphenylmandelate + NADH + H+
show the reaction diagram
-
-
-
-
?
3,4-dihydroxyphenylacetaldehyde + NAD+
3,4-dihydroxyphenylacetate + NADH
show the reaction diagram
-
-
-
-
?
3,4-dihydroxyphenylacetaldehyde + NAD+ + H2O
3,4-dihydroxyphenylacetate + NADH + H+
show the reaction diagram
-
-
-
?
3,4-dihydroxyphenylglycolaldehyde + NAD+ + H2O
3,4-dihydroxyphenylglycolate + NADH + H+
show the reaction diagram
-
-
-
?
3-chloroallylaldehyde + NAD+ + H2O
? + NADH + H+
show the reaction diagram
-
-
-
-
?
3-chloroallylaldehyde + NADP+ + H2O
? + NADPH + H+
show the reaction diagram
-
-
-
-
?
3-hydroxybenzaldehyde + NADP+ + H2O
3-hydroxybenzoate + NADPH + H+
show the reaction diagram
-
-
-
-
?
3-hydroxypropionaldehyde + NAD+ + H2O
3-hydroxypropanoate + NADH + 2 H+
show the reaction diagram
3-nitrobenzaldehyde + NAD+ + H2O
3-nitrobenzoate + NADH + H+
show the reaction diagram
32.98% of the activity compared to acetaldehyde
-
-
?
4-bromobenzaldehyde + NAD+ + H2O
4-bromobenzoate + NADH + H+
show the reaction diagram
-
-
-
-
?
4-carboxybenzaldehyde + NADP+ + H2O
4-carboxybenzoate + NADPH + H+
show the reaction diagram
-
-
-
-
?
4-chlorobenzaldehyde + NADP+ + H2O
4-chlorobenzoate + NADPH + H+
show the reaction diagram
-
-
-
-
?
4-fluorobenzaldehyde + NAD+ + H2O
4-fluorobenzoate + NADH + H+
show the reaction diagram
-
-
-
-
?
4-formylpyrene + NADP+ + H2O
pyrene-4-carboxylic acid + NADPH + H+
show the reaction diagram
-
-
-
?
4-hydroxy-2-nonenal + NAD(P)+ + H2O
? + NAD(P)H + H+
show the reaction diagram
-
-
-
-
?
4-nitrobenzaldehyde + NAD+ + H2O
4-nitrobenzoate + NADH + H+
show the reaction diagram
-
-
-
-
?
4-pyridinecarboxaldehyde + NAD+ + H2O
4-pyridinecarboxylate + NADH + H+
show the reaction diagram
-
-
-
-
?
5-hydroxyindole-3-acetyldehyde + NAD+ + H2O
5-hydroxyindole-3-acetate + NADH + H+
show the reaction diagram
-
-
-
-
?
6-methoxy-2-naphthaldehyde + NADH + H+
6-methoxy-2-naphthyl alcohol + NAD+
show the reaction diagram
-
-
-
-
?
acetaldehyde + 3-pyridine aldehyde adenine dinucleotide phosphate
acetate + ?
show the reaction diagram
-
-
-
-
?
acetaldehyde + acetylpyridine adenine dinucleotide
acetate + ?
show the reaction diagram
-
-
-
-
?
acetaldehyde + deamino-NAD+ + H2O
acetate + deamino-NADH + H+
show the reaction diagram
-
-
-
-
?
acetaldehyde + NAD(P)+ + H2O
acetate + NAD(P)H + H+
show the reaction diagram
acetaldehyde + NAD+
acetate + NADH + H+
show the reaction diagram
-
-
-
-
?
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
acetaldehyde + NADP+
acetate + NADPH + H+
show the reaction diagram
-
-
-
-
?
acetaldehyde + NADP+ + H2O
acetate + NADPH
show the reaction diagram
-
-
-
?
acetaldehyde + NADP+ + H2O
acetate + NADPH + H+
show the reaction diagram
acetaldehyde + thio-NAD+ + H2O
acetate + thio-NADH + H+
show the reaction diagram
-
-
-
-
?
acetaldehyde + thio-NADP+
acetate + ?
show the reaction diagram
-
-
-
-
?
aldophosphamide + NAD+
N,N-bis-(2-chloroethyl)-diaminophosphoric-(2-carboxyethylester) + ?
show the reaction diagram
-
-
-
-
?
all-trans-retinal + NAD+ + H2O
all-trans-retinoic acid + NADH + H+
show the reaction diagram
all-trans-retinal + NADP+ + H2O
all-trans-retinol + NADPH + H+
show the reaction diagram
an aldehyde + NAD(P)+ + H2O
an acid + NAD(P)H + H+
show the reaction diagram
an aldehyde + NAD+ + H2O
a carboxylate + NADH + H+
show the reaction diagram
an aldehyde + NADP+ + H2O
a carboxylate + NADPH + H+
show the reaction diagram
benzaldehyde + NAD+ + H2O
benzoate + NADH + H+
show the reaction diagram
benzaldehyde + NADH + H+
benzyl alcohol + NAD+
show the reaction diagram
-
-
-
-
?
benzaldehyde + NADP+
benzoate + NADPH
show the reaction diagram
benzaldehyde + NADP+ + H2O
benzoate + NADPH
show the reaction diagram
benzaldehyde + NADP+ + H2O
benzoate + NADPH + H+
show the reaction diagram
butyraldehyde + NAD(P)+ + H2O
butanoate + NAD(P)H + H+
show the reaction diagram
butyraldehyde + NAD+
butyrate + NADH + H+
show the reaction diagram
-
-
-
-
?
butyraldehyde + NAD+ + H2O
butyrate + NADH + H+
show the reaction diagram
butyraldehyde + NADP+
butyrate + NADPH + H+
show the reaction diagram
-
-
-
-
?
chloroacetaldehyde + NAD+ + H2O
chloroacetate + NADH + H+
show the reaction diagram
cinnamaldehyde + NAD+ + H2O
cinnamate + NADH + H+
show the reaction diagram
-
-
-
-
?
cinnamaldehyde + NADH + H+
cinnamyl alcohol + NAD+
show the reaction diagram
-
-
-
-
?
D-glyceraldehyde + NAD+ + H2O
D-glycerate 3-phosphate + NADH + H+
show the reaction diagram
50.25% of the activity compared to acetaldehyde. kcat/KM for NAD+ with D-glyceraldehyde as cosubstrate is 3.8fold higher compared to NADP+
-
-
?
D-glyceraldehyde + NADP+ + H2O
D-glycerate + NADPH
show the reaction diagram
50.25% of the activity compared to acetaldehyde
-
-
?
D-glyceraldehyde 3-phosphate + NAD+ + H2O
D-glycerate 3-phosphate + NADH + H+
show the reaction diagram
52.7% of the activity compared to acetaldehyde
-
-
?
DL-glyceraldehyde + NAD(P)+ + H2O
glycerate + NAD(P)H + H+
show the reaction diagram
-
14% of activity with benzaldehyde
-
-
ir
dodecanal + NADP+ + H2O
dodecanoate + NADPH + H+
show the reaction diagram
-
-
-
-
?
formaldehyde + NAD(P)+ + H2O
formate + NAD(P)H + H+
show the reaction diagram
formaldehyde + NAD+ + H2O
formate + NADH + H+
show the reaction diagram
formaldehyde + NADP+ + H2O
formate + NADPH + H+
show the reaction diagram
furfuryl aldehyde + NAD+ + H2O
2-furancarboxylate + NADH + H+
show the reaction diagram
glutaraldehyde + NAD+
glutarate + NADH
show the reaction diagram
-
-
-
-
?
glyceraldehyde + NAD(P)+ + H2O
glycerate + NAD(P)H + H+
show the reaction diagram
-
-
-
ir
glyceraldehyde + NAD+ + H2O
glycerate + NADH + H+
show the reaction diagram
-
-
-
-
?
glycolaldehyde + NAD+
glycolate + NADH
show the reaction diagram
glycolaldehyde + NAD+ + H2O
glycolate + NADH + H+
show the reaction diagram
75.43% of the activity compared to acetaldehyde
-
-
?
glycolaldehyde + NADP+ + H2O
glycolate + NADPH + H+
show the reaction diagram
heptanal + NADP+ + H2O
heptanoate + NADPH + H+
show the reaction diagram
-
-
-
?
heptylaldehyde + NAD(P)+ + H2O
heptanoate + NAD(P)H + H+
show the reaction diagram
hexanal + NAD+ + H2O
hexanoate + NADH + H+
show the reaction diagram
hexylaldehyde + NAD(P)+ + H2O
hexanoate + NAD(P)H + H+
show the reaction diagram
indole-3-acetaldehyde + NAD+ + H2O
indole-3-acetate + NADH + H+
show the reaction diagram
-
-
-
?
isobutanal + NAD+ + H2O
isobutyrate + NADH + H+
show the reaction diagram
isobutyraldehyde + NAD(P)+ + H2O
isobutanoate + NAD(P)H + H+
show the reaction diagram
isopentanal + NAD+ + H2O
isopentanoate + NADH + H+
show the reaction diagram
-
-
-
-
?
isovaleraldehyde + NAD(P)+ + H2O
isovalerate + NAD(P)H
show the reaction diagram
isovaleraldehyde + NAD+
isovalerate + NADH + H+
show the reaction diagram
-
-
-
-
?
isovaleraldehyde + NADP+
isovalerate + NADPH + H+
show the reaction diagram
isovaleraldehyde + NADP+ + H2O
isovalerate + NADPH + H+
show the reaction diagram
-
-
-
-
m-bromobenzaldehyde + NAD(P)+ + H2O
m-bromobenzoate + NAD(P)H + H+
show the reaction diagram
m-chlorobenzaldehyde + NAD(P)+ + H2O
m-chlorobenzoate + NAD(P)H + H+
show the reaction diagram
m-fluorobenzaldehyde + NAD(P)+ + H2O
m-fluorobenzoate + NAD(P)H + H+
show the reaction diagram
mafosfamide + NADP+ + H2O
?
show the reaction diagram
-
-
-
-
?
malondialdehyde + NAD(P)+ + H2O
? + NAD(P)H + H+
show the reaction diagram
-
-
-
-
?
malondialdehyde + NAD+
? + NADH
show the reaction diagram
malonic semialdehyde + NAD+
?
show the reaction diagram
-
-
-
-
?
n-butanal + NAD+ + H2O
butanoate + NADH + H+
show the reaction diagram
n-butanal + NADP+ + H2O
n-butanoate + NADPH + H+
show the reaction diagram
n-decanal + NADP+ + H2O
n-decanoate + NADPH + H+
show the reaction diagram
n-dodecanal + NADP+ + H2O
n-dodecanoate + NADPH + H+
show the reaction diagram
-
-
-
-
?
n-hexanal + NADP+ + H2O
n-hexanoate + NADPH + H+
show the reaction diagram
-
-
-
-
?
n-nonanal + NADP+ + H2O
n-nonanoate + NADPH + H+
show the reaction diagram
-
-
-
-
?
n-octanal + NADP+ + H2O
n-octanoate + NADPH + H+
show the reaction diagram
-
-
-
-
?
n-pentanal + NAD+ + H2O
n-pentanoate + NADH + H+
show the reaction diagram
n-pentanal + NADP+ + H2O
n-pentanoate + NADPH + H+
show the reaction diagram
-
-
-
-
?
n-propanal + NADP+
propionate + NADPH + H+
show the reaction diagram
-
-
-
-
?
n-propanal + NADP+ + H2O
n-propanoate + NADPH + H+
show the reaction diagram
-
-
-
-
?
n-tetradecanal + NADP+ + H2O
n-tetradecanoate + NADPH + H+
show the reaction diagram
-
-
-
-
?
n-tridecanal + NADP+ + H2O
n-tridecanoate + NADPH + H+
show the reaction diagram
-
-
-
-
?
n-undecanal + NADP+ + H2O
n-undecanoate + NADPH + H+
show the reaction diagram
-
-
-
-
?
nonanal + NADP+ + H2O
nonanoate + NADPH + H+
show the reaction diagram
-
-
-
-
?
o-fluorobenzaldehyde + NAD(P)+ + H2O
o-fluorobenzoate + NAD(P)H + H+
show the reaction diagram
o-methoxybenzaldehyde + NAD(P)+ + H2O
o-methoxybenzoate + NAD(P)H + H+
show the reaction diagram
octanal + NAD+ + H2O
octanoate + NADH + H+
show the reaction diagram
octylaldehyde + NAD(P)+ + H2O
octanoate + NAD(P)H + H+
show the reaction diagram
p-bromobenzaldehyde + NAD(P)+ + H2O
p-bromobenzoate + NAD(P)H + H+
show the reaction diagram
p-chlorobenzaldehyde + NAD(P)+ + H2O
p-chlorobenzoate + NAD(P)H + H+
show the reaction diagram
p-fluorobenzaldehyde + NAD(P)+ + H2O
p-fluorobenzoate + NAD(P)H + H+
show the reaction diagram
p-hydroxybenzaldehyde + NADP+ + H2O
p-hydroxybenzoic acid + NADPH + H+
show the reaction diagram
-
-
-
-
?
p-isopropylbenzaldehyde + NAD(P)+ + H2O
p-isopropylbenzoate + NAD(P)H + H+
show the reaction diagram
p-nitrobenzaldehyde + NADP+ + H2O
p-nitrobenzoate + NADPH + H+
show the reaction diagram
-
-
-
-
?
phenylacetaldehyde + NAD+ + H2O
phenylacetate + NADH + H+
show the reaction diagram
-
-
-
-
?
phenylacetaldehyde + NADP+ + H2O
phenylacetate + NADPH + H+
show the reaction diagram
-
-
-
-
?
polyethylene glycol 1000-aldehyde + NADP+
? + NADPH
show the reaction diagram
-
-
-
-
?
polyethylene glycol 200-aldehyde + NADP+
? + NADPH
show the reaction diagram
-
-
-
-
?
polyethylene glycol 400-aldehyde + NADP+
? + NADPH
show the reaction diagram
-
-
-
-
?
polyethylene glycol 4000-aldehyde + NADP+
? + NADPH
show the reaction diagram
-
highest activtiy compared to other polyethylene glycolaldehydes
-
-
?
polyethylene glycol 6000-aldehyde + NADP+
? + NADPH
show the reaction diagram
-
-
-
-
?
propanal + 1,N6-ethenoadenine dinucleotide
propionate + ?
show the reaction diagram
-
isoenzyme E1 and E2
-
-
?
propanal + 3-acetylpyridine adenine dinucleotide
propionate + ?
show the reaction diagram
-
isoenzyme E1 and E2
-
-
?
propanal + 3-pyridine adenine dinucleotide
propionate + ?
show the reaction diagram
-
isoenzyme E1 and E2
-
-
?
propanal + 3-thionicotinamide adenine dinucleotide
propionate + ?
show the reaction diagram
propanal + beta-NADP+ + H2O
propionate + beta-NADPH + H+
show the reaction diagram
-
isoenzyme E1 and E2
-
-
?
propanal + N-guanine dinucleotide
propionate + ?
show the reaction diagram
-
isoenzyme E1 and E2
-
-
?
propanal + N-hypoxanthine dinucleotide
propionate + ?
show the reaction diagram
-
isoenzyme E1 and E2
-
-
?
propanal + NAD+ + H2O
propionate + NADH + H+
show the reaction diagram
propionaldehyde + NAD(P)+ + H2O
propionate + NAD(P)H
show the reaction diagram
propionaldehyde + NAD+ + H2O
propionate + NADH + H+
show the reaction diagram
propionaldehyde + NADP+ + H2O
propionate + NADPH + H+
show the reaction diagram
retinal + NAD+ + H2O
retinoic acid + NADH + H+
show the reaction diagram
preferred substrate of isozyme ALDH1A3
-
-
?
succinic semialdehyde + NAD+
? + NADH
show the reaction diagram
-
-
-
-
?
trans-2-hexenal + NAD+
trans-2-hexenoate + NADH
show the reaction diagram
valeraldehyde + NAD(P)+ + H2O
valerate + NAD(P)H + H+
show the reaction diagram
valeraldehyde + NAD+
valerate + NADH + H+
show the reaction diagram
-
-
-
-
?
valeraldehyde + NADP+
valerate + NADPH + H+
show the reaction diagram
valeraldehyde + NADP+ + H2O
valerate + NADPH + H+
show the reaction diagram
-
-
-
?
additional information
?
-
top print hide
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3-chloroallylaldehyde + NAD+ + H2O
? + NADH + H+
show the reaction diagram
-
-
-
-
?
3-chloroallylaldehyde + NADP+ + H2O
? + NADPH + H+
show the reaction diagram
-
-
-
-
?
4-hydroxy-2-nonenal + NAD(P)+ + H2O
? + NAD(P)H + H+
show the reaction diagram
-
-
-
-
?
acetaldehyde + NAD(P)+ + H2O
acetate + NAD(P)H + H+
show the reaction diagram
-
-
-
-
ir
an aldehyde + NAD(P)+ + H2O
an acid + NAD(P)H + H+
show the reaction diagram
an aldehyde + NAD+ + H2O
a carboxylate + NADH + H+
show the reaction diagram
an aldehyde + NADP+ + H2O
a carboxylate + NADPH + H+
show the reaction diagram
chloroacetaldehyde + NAD+ + H2O
chloroacetate + NADH + H+
show the reaction diagram
-
-
-
-
?
malondialdehyde + NAD(P)+ + H2O
? + NAD(P)H + H+
show the reaction diagram
-
-
-
-
?
propionaldehyde + NAD+ + H2O
propionate + NADH + H+
show the reaction diagram
-
-
-
-
?
additional information
?
-
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADH
-
-
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
; stimulating
CaCl2
-
30 mM, 3.8fold stimulation of activity of ALDH1
KCl
-
120 mM: 40fold increase of activity of ALDH5 with NAD+. 16 mM: 20fold increase of activity of enzyme ALDH5 with NADP+. No stimulation of the activity of enzyme form ALDH1
Li+
-
500 mM, 107% of the activity compared to activity without metal ions
MnCl2
-
2 mM, 2.1fold stimulation of activity of ALDH1
Na+
-
500 mM, 1.2fold activation
additional information
-
the enzyme does not require metal ions for its enzymatic reaction
top print hide
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,2-Cyclohexanedione
-
33% residual activity at 2.5 mM
2,4-Dinitro-1-fluorobenzene
-
11% residual activity at 2.5 mM
2-mercaptoethanol
-
10% (v/v), 55% inhibition
3-thionicotinamide adenine dinucleotide
-
strong inhibition versus beta-NAD+, isoenzyme E2; substrate inhibition, isoenzyme E1
4-hydroxy-2-nonenal
-
-
acetaldehyde
-
strong substrate inhibition
acetone
-
10% (v/v), 21% inhibition
benomyl
potent irreversible inhibitor of ALDH. Benomyl increases superoxide production as much as nitroglycerin
Benzoate
-
non-competitive with respect to either NAD+ or benzaldehyde
beta-NADP+
-
substrate inhibition, isoenzyme E1
Cd2+
-
100% inhibition at 1 mM; 1 mM, 100% inhibition
Chloral hydrate
Co2+
-
89% residual activity at 1 mM
diethyl dicarbonate
-
2% residual activity at 2.5 mM
dimethyl ampal thiolester
-
complete inhibition of isozyme ALDH3 at 0.0075 mM
Disulfiram
DMSO
-
10% (v/v), 32% inhibition
EDTA
-
50 mM, 22% inhibition
ethanediol
-
10% (v/v), 60% inhibition
ethyl acetimidate
-
71% residual activity at 250 mM
Fe2+
-
88% residual activity at 1 mM
guanidine hydrochloride
-
10% (v/v), 42% inhibition
H2O2
-
the loss of activity induced by H2O2 can be restored by reducing agents, although a decrease of the restored activity is observed upon addition of 10 mM dithiothreitol
methyl ampal thiolester
-
78% inhibition of isozyme ALDH3 at 0.0075 mM
Mg2+
-
89% residual activity at 1 mM
Mn2+
-
89% residual activity at 1 mM
N-Acetylimidazole
-
75% residual activity at 2.5 mM
N-bromosuccinimide
-
66% residual activity at 0.5 mM
nitroglycerin
p-chloromercuribenzoate
-
50 microM, 80% inhibition
p-hydroxymercuribenzoate
p-mercuribenzoate
-
80% inhibition at 0.05 mM
Pb2+
-
100% inhibition at 1 mM; 1 mM, 100% inhibition
PEG-400
-
10% (v/v), 16% inhibition
pentanal
-
extremely strong substrate inhibition
phenylmethylsulfonyl fluoride
-
73% residual activity at 2.5 mM
PMSF
-
50 mM, no loss of activity
pyrazole
-
100% inhibition at 1 mM; 1 mM, 100% inhibition
trans-4-(N,N-dimethylamino)-cinnamaldehyde
-
1 mM, 58% inhibition; 58% inhibition at 1 mM
Urea
-
500 mM, 44% inhibition
Woodward's reagent K
-
71% residual activity at 50 mM
ZnCl2
-
50% inhibition at 0.035 mM, enzyme form ALDH1
additional information
-
not inhibited by EDTA
-
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
-
246% activity at 2 mM
dithiothreitol
-
276% activity at 2 mM
ethanol
-
0.05 mM, 1.8fold stimulation
H2O2
-
0.015 mM, 1.2fold stimulation
NH4+
-
500 mM, 1.31fold activation
Sodium dodecyl sulfate
-
0.01 mM, 1.1fold stimulation
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.096
1,N6-ethenoadenine dinucleotide
-
isoenzyme E2
0.0016
1-formyl-6-methylpyrene
-
ALDH3A1, at pH 7.5 and 37°C
0.004
1-formyl-8-methylpyrene
-
ALDH3A1,at pH 7.5 and 37°C
0.004
1-formylpyrene
-
ALDH3A1, at pH 7.5 and 37°C
0.012 - 0.379
2-Bromobenzaldehyde
0.006 - 0.063
2-Chlorobenzaldehyde
0.008 - 0.822
2-Fluorobenzaldehyde
0.011
2-formylpyrene
-
ALDH3A1, at pH 7.5 and 37°C
0.0007
2-naphthaldehyde
-
pH 7.5, 25°C
0.39
3,4-dihydroxyphenyl acetaldehyde
-
reaction with NAD+
0.61
3,4-dihydroxyphenyl benzaldehyde
-
reaction with NAD+
0.0004 - 0.015
3,4-dihydroxyphenylacetaldehyde
0.0132 - 0.62
3-acetylpyridine adenine dinucleotide
0.0338 - 0.504
3-pyridinealdehyde adenine dinucleotide
0.0151
3-thionicotinamide adenine dinucleotide
-
isoenzyme E1
0.039 - 0.617
4-bromobenzaldehyde
0.031 - 1.159
4-Chlorobenzaldehyde
0.012 - 2.057
4-Fluorobenzaldehyde
0.00078
4-formylpyrene
-
ALDH3A1, at pH 7.5 and 37°C
0.03
4-hydroxynonenal
-
-
0.081 - 1.103
4-iodobenzaldehyde
0.091 - 0.19
4-Pyridinecarboxaldehyde
0.15
5-hydroxyindole-3-acetaldehyde
-
reaction with NAD+
0.0033
6-methoxy-2-naphthaldehyde
-
pH 7.5, 25°C
0.0002 - 393.4
acetaldehyde
0.64
aldophosphamide
-
reaction with NAD+
0.007
all-trans-retinal
-
with NAD+ as cosubstrate, at pH 7.0 and 37°C
0.013 - 4.15
benzaldehyde
0.0047
beta-NAD+
-
-
7.68
beta-NADP+
-
isoenzyme E1
0.094
betaNAD+
-
isoenzyme E2
2.95 - 4.93
Butyraldehyde
0.01 - 0.46
Chloroacetaldehyde
0.0053
cinnamaldehyde
-
pH 7.5, 25°C
8.51
D-glyceraldehyde
-
pH 8, 80°C
6.12
D-glyceraldehyde 3-phosphate
-
pH 8, 80°C
0.27 - 1.9
formaldehyde
0.05 - 0.13
glycolaldehyde
0.06 - 0.08
Hexanal
0.5
Indole-3-acetaldehyde
-
reaction with NAD+
0.016
Isobutyraldehyde
30°C, pH 10.0
0.0005
isopentanal
-
isoenzyme F1
1.66 - 10.78
Isovaleraldehyde
0.0113
N-1,N6-ethenoadenine dinucleotide
-
isoenzyme E1
0.137
n-dodecanal
-
50 mM potassium phosphate buffer pH8.0, 1 mM CaCl2, 36°C
1.33
N-guanine dinucleotide
-
isoenzyme E1
0.121 - 1.36
N-hypoxanthine dinucleotide
0.003 - 35
NAD+
0.01 - 260
NADP+
0.067 - 0.113
octanal
0.0001
pentanal
-
isoenzyme F1
0.0003 - 0.004
phenylacetaldehyde
0.00039 - 19.06
propanal
0.46 - 12
propionaldehyde
0.1 - 0.12
trans-2-hexenal
0.12 - 0.34
Valeraldehyde
additional information
additional information
-
-
-
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
26.1
4-hydroxynonenal
-
-
0.183 - 35.9
acetaldehyde
0.005
all-trans-retinal
-
with NAD+ as cosubstrate, at pH 7.0 and 37°C
0.217 - 51
benzaldehyde
11.5 - 32.8
Butyraldehyde
0.47 - 7.7
Chloroacetaldehyde
0.92
D-glyceraldehyde
-
pH 8, 80°C
0.83
D-glyceraldehyde 3-phosphate
-
pH 8, 80°C
34
Hexanal
0.91 - 3.58
Isovaleraldehyde
0.0007 - 45.3
NAD+
3.71 - 38.8
NADP+
13.36 - 49.38
propionaldehyde
35.1 - 53
trans-2-hexenal
19.41 - 30.99
Valeraldehyde
additional information
additional information
-
-
-
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.003 - 1.85
acetaldehyde
0.67
all-trans-retinal
-
with NAD+ as cosubstrate, at pH 7.0 and 37°C
1.08
benzaldehyde
-
at pH 7.0 and 37°C
2.39 - 11.89
Butyraldehyde
5.5 - 200
Chloroacetaldehyde
0.11
D-glyceraldehyde
-
pH 8, 80°C
0.14
D-glyceraldehyde 3-phosphate
-
pH 8, 80°C
0.09 - 0.36
Isovaleraldehyde
0.16 - 158.3
NAD+
0.974 - 0.98
NADP+
20.67 - 107.5
propionaldehyde
56.66 - 257
Valeraldehyde
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
6.9
acetaldehyde
-
-
0.19 - 0.2
Chloral hydrate
0.012 - 0.069
Disulfiram
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.07
-
cell free extract
0.212
-
activity with NADP+
0.35
-
isoenzyme F1
0.378
-
activity with NAD+
0.42
-
activity in isolated mitochondria
0.49
-
after the Ni-NTA purification step
1
-
isoenzyme F2
1.48
-
recombinant enzyme, using 3-hydroxypropionaldehyde and NAD+ as substrates, at pH 8.0 and 37°C
5.51
-
-
16.67
-
enzyme from breast adenocarcinoma cell line
18.92
-
-
32.95
-
enzyme from stomach mucosa
110.5
-
liver enzyme
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8 - 8.5
-
benzaldehyde oxidation, enzyme from breast adenocarcinoma cell line
8 - 9
-
benzaldehyde oxidation, enzyme from stomach mucosa
9 - 9.5
-
-
9.3
-
reaction with propanal and NAD+ or NADP+
10
-
activity with NAD+, broad plateau from pH 5 to 8 followed by a sharp increase in activity to pH 10
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 9
-
pH 6.0: about 60% of maximal activity, pH 9.0: about 90% of maximal activity
6.2 - 6.7
-
pH 6.2: about 80% of maximal activity, pH 6.7: about 80% of maximal activity
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
36
-
; activity assay
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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
70 - 90
-
70°C: about 50% of maximal activity, 90°C: about 90% of maximal activity
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
exhibiting oxazaphosphorine-specific acquired resistance, enzyme form ALDH3
Manually annotated by BRENDA team
-
isoform ALDH3A1
Manually annotated by BRENDA team
isozyme Aldh1a3 mRNA expression is highest in placenta
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
additional information
addition of ALDH to RASM cell homogenates significantly decreases NO consumption
Manually annotated by BRENDA team
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
isoform ALDH3I1
Manually annotated by BRENDA team
-
isoforms ALDH3F1 and ALDH3H1
Manually annotated by BRENDA team
-
enzyme III
-
Manually annotated by BRENDA team
-
isoforms ALDH3I1 and ALDH3H1
Manually annotated by BRENDA team
-
isoform ALDH3H1
Manually annotated by BRENDA team
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