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Literature summary for 1.2.1.5 extracted from

  • Wang, X.; Mann, C.J.; Bai, Y.; Ni, L.; Weiner, H.
    Molecular cloning, characterization, and potential roles of cytosolic and mitochondrial aldehyde dehydrogenases in ethanol metabolism in Saccharomyces cerevisiae (1998), J. Bacteriol., 180, 822-830.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.1.5

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
ZnCl2 50% inhibition at 0.035 mM, enzyme form ALDH1 Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 acetaldehyde reaction with NADP+, enzyme form ALDH2 Saccharomyces cerevisiae
0.013
-
 propanal reaction with NAD+, enzyme form ALDH2 Saccharomyces cerevisiae
0.017
-
 propanal reaction with NADP+, enzyme form ALDH2 Saccharomyces cerevisiae
0.024
-
 acetaldehyde reaction with NADP+, enzyme form ALDH1 Saccharomyces cerevisiae
0.03
-
 propanal reaction with NADP+, enzyme form ALDH1 Saccharomyces cerevisiae
0.04
-
 NADP+ reaction with acetaldehyde, enzyme form ALDH1 Saccharomyces cerevisiae
0.058
-
 acetaldehyde reaction with NADP+, enzyme form ALDH5 Saccharomyces cerevisiae
0.099
-
 NADP+ reaction with propanal, enzyme form ALDH1 Saccharomyces cerevisiae
0.39
-
 propanal reaction with NADP+, enzyme form ALDH5 Saccharomyces cerevisiae
0.447
-
 NADP+ reaction with propanal, enzyme form ALDH2 Saccharomyces cerevisiae
0.64
-
 NADP+ reaction with acetaldehyde, enzyme form ALDH5 Saccharomyces cerevisiae
0.7
-
 propanal reaction with NAD+, enzyme form ALDH1 Saccharomyces cerevisiae
0.83
-
 propanal reaction with NAD+, enzyme form ALDH5 Saccharomyces cerevisiae
1.1
-
 NAD+ reaction with propanal, enzyme form ALDH2 Saccharomyces cerevisiae
1.4
-
 NADP+ reaction with acetaldehyde, enzyme form ALDH2 Saccharomyces cerevisiae
3.47
-
 NADP+ reaction with propanal, enzyme form ALDH5 Saccharomyces cerevisiae
6.43
-
 NAD+ reaction with propanal, enzyme form ALDH5 Saccharomyces cerevisiae
17.4
-
 NAD+ reaction with propanal, enzyme form ALDH1 Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol enzyme form ALDH1 Saccharomyces cerevisiae 5829
-
mitochondrion enzyme form ALDH5 Saccharomyces cerevisiae 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
CaCl2 30 mM, 3.8fold stimulation of activity of ALDH1 Saccharomyces cerevisiae
KCl 120 mM: 40fold increase of activity of ALDH5 with NAD+. 16 mM: 20fold increase of activity of enzyme ALDH5 with NADP+. No stimulation of the activity of enzyme form ALDH1 Saccharomyces cerevisiae
Mg2+ 20 mM MgCl2, 2.8fold stimulation of activity of ALDH1 Saccharomyces cerevisiae
MnCl2 2 mM, 2.1fold stimulation of activity of ALDH1 Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53904
-
 x * 53904, enzyme form ALDH5, mass spectrometric analysis Saccharomyces cerevisiae
54570
-
 x * 54570, enzyme form ALDH1, mass spectrometric analysis Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
 ALDH1, ALDH2 and ALDH5
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + NAD+ + H2O
-
 Saccharomyces cerevisiae acetate + NADH + H+
-
 ?
benzaldehyde + NAD+ + H2O ALDH2 is very active, low activity with ALDH1 and ALDH5 Saccharomyces cerevisiae benzoate + NADH + H+
-
 ?
propanal + NAD+ + H2O
-
 Saccharomyces cerevisiae propionate + NADH + H+
-
 ?

Subunits

Subunits Comment Organism
? x * 53904, enzyme form ALDH5, mass spectrometric analysis Saccharomyces cerevisiae
? x * 54570, enzyme form ALDH1, mass spectrometric analysis Saccharomyces cerevisiae