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Literature summary for 1.2.1.5 extracted from

  • Hong, S.H.; Ngo, H.P.; Nam, H.K.; Kim, K.R.; Kang, L.W.; Oh, D.K.
    Alternative biotransformation of retinal to retinoic acid or retinol by an aldehyde dehydrogenase from Bacillus cereus (2016), Appl. Environ. Microbiol., 82, 3940-3946.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.1.5

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli ER2566 cells Bacillus cereus

Protein Variants

Protein Variants Comment Organism
C300A the variant shows no oxidation activity Bacillus cereus
E194S the variant shows 15fold higher catalytic efficiency (kcat/Km) for the reduction of all-trans-retinal than the wild type enzyme Bacillus cereus
E266A the variant shows no oxidation activity Bacillus cereus
E457V the variant shows 7.5fold higher catalytic efficiency (kcat/Km) for the reduction of all-trans-retinal than the wild type enzyme Bacillus cereus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0037
-
 NAD+ mutant enzyme C300S, with all-trans-retinal as cosubstrate, at pH 7.0 and 37°C Bacillus cereus
0.004
-
 NAD+ wild type enzyme, with all-trans-retinal as cosubstrate, at pH 7.0 and 37°C Bacillus cereus
0.00625
-
 NAD+ mutant enzyme E457V, with all-trans-retinal as cosubstrate, at pH 7.0 and 37°C Bacillus cereus
0.007
-
 all-trans-retinal with NAD+ as cosubstrate, at pH 7.0 and 37°C Bacillus cereus
0.01
-
 NAD+ mutant enzyme E194S, with all-trans-retinal as cosubstrate, at pH 7.0 and 37°C Bacillus cereus
0.095
-
 acetaldehyde at pH 7.0 and 37°C Bacillus cereus
0.2
-
 benzaldehyde at pH 7.0 and 37°C Bacillus cereus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
 4 * 54000, SDS-PAGE Bacillus cereus
216000
-
 gel filtration Bacillus cereus

Organism

Organism UniProt Comment Textmining
Bacillus cereus A0A150BLG9
-
-
Bacillus cereus ATCC 10876 A0A150BLG9
-
-

Purification (Commentary)

Purification (Comment) Organism
Sephacryl S-300 gel filtration Bacillus cereus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + NAD+ + H2O
-
 Bacillus cereus acetate + NADH + H+
-
 ?
acetaldehyde + NAD+ + H2O
-
 Bacillus cereus ATCC 10876 acetate + NADH + H+
-
 ?
all-trans-retinal + NAD+ + H2O
-
 Bacillus cereus all-trans-retinoic acid + NADH + H+
-
 ?
all-trans-retinal + NAD+ + H2O
-
 Bacillus cereus ATCC 10876 all-trans-retinoic acid + NADH + H+
-
 ?
all-trans-retinal + NADP+ + H2O
-
 Bacillus cereus all-trans-retinol + NADPH + H+
-
 ?
all-trans-retinal + NADP+ + H2O
-
 Bacillus cereus ATCC 10876 all-trans-retinol + NADPH + H+
-
 ?
benzaldehyde + NAD+ + H2O
-
 Bacillus cereus benzoate + NADH + H+
-
 ?
benzaldehyde + NAD+ + H2O
-
 Bacillus cereus ATCC 10876 benzoate + NADH + H+
-
 ?
additional information no activity with 9-cis-retinal and 13-cis-retinal Bacillus cereus ?
-
 ?
additional information no activity with 9-cis-retinal and 13-cis-retinal Bacillus cereus ATCC 10876 ?
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 54000, SDS-PAGE Bacillus cereus

Synonyms

Synonyms Comment Organism
ALDH
-
 Bacillus cereus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0007
-
 NAD+ mutant enzyme C300S, with all-trans-retinal as cosubstrate, at pH 7.0 and 37°C Bacillus cereus
0.0017
-
 NAD+ wild type enzyme, with all-trans-retinal as cosubstrate, at pH 7.0 and 37°C Bacillus cereus
0.005
-
 NAD+ mutant enzyme E194S, with all-trans-retinal as cosubstrate, at pH 7.0 and 37°C Bacillus cereus
0.005
-
 all-trans-retinal with NAD+ as cosubstrate, at pH 7.0 and 37°C Bacillus cereus
0.026
-
 NAD+ mutant enzyme E457V, with all-trans-retinal as cosubstrate, at pH 7.0 and 37°C Bacillus cereus
0.183
-
 acetaldehyde at pH 7.0 and 37°C Bacillus cereus
0.217
-
 benzaldehyde at pH 7.0 and 37°C Bacillus cereus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Bacillus cereus
NADP+
-
 Bacillus cereus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.16
-
 NAD+ mutant enzyme C300S, with all-trans-retinal as cosubstrate, at pH 7.0 and 37°C Bacillus cereus
0.42
-
 NAD+ wild type enzyme, with all-trans-retinal as cosubstrate, at pH 7.0 and 37°C Bacillus cereus
0.5
-
 NAD+ mutant enzyme E194S, with all-trans-retinal as cosubstrate, at pH 7.0 and 37°C Bacillus cereus
0.67
-
 all-trans-retinal with NAD+ as cosubstrate, at pH 7.0 and 37°C Bacillus cereus
1.08
-
 benzaldehyde at pH 7.0 and 37°C Bacillus cereus
1.85
-
 acetaldehyde at pH 7.0 and 37°C Bacillus cereus
4.1
-
 NAD+ mutant enzyme E457V, with all-trans-retinal as cosubstrate, at pH 7.0 and 37°C Bacillus cereus