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Literature summary for 1.2.1.5 extracted from

  • Lo, H.F.; Chen, Y.J.
    Gene cloning and biochemical characterization of a NAD(P)+ -dependent aldehyde dehydrogenase from Bacillus licheniformis (2010), Mol. Biotechnol., 46, 157-167.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 246% activity at 2 mM Bacillus licheniformis
dithiothreitol 276% activity at 2 mM Bacillus licheniformis

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli M15(pREP4) cells Bacillus licheniformis

Inhibitors

Inhibitors Comment Organism Structure
1,2-Cyclohexanedione 33% residual activity at 2.5 mM Bacillus licheniformis
2,4-Dinitro-1-fluorobenzene 11% residual activity at 2.5 mM Bacillus licheniformis
Co2+ 89% residual activity at 1 mM Bacillus licheniformis
Cu2+ 20% residual activity at 1 mM Bacillus licheniformis
diethyl dicarbonate 2% residual activity at 2.5 mM Bacillus licheniformis
ethyl acetimidate 71% residual activity at 250 mM Bacillus licheniformis
Fe2+ 88% residual activity at 1 mM Bacillus licheniformis
Hg2+ complete inhibition at 0.25 mM Bacillus licheniformis
Mg2+ 89% residual activity at 1 mM Bacillus licheniformis
Mn2+ 89% residual activity at 1 mM Bacillus licheniformis
additional information not inhibited by EDTA Bacillus licheniformis
N-Acetylimidazole 75% residual activity at 2.5 mM Bacillus licheniformis
N-bromosuccinimide 66% residual activity at 0.5 mM Bacillus licheniformis
phenylmethylsulfonyl fluoride 73% residual activity at 2.5 mM Bacillus licheniformis
Woodward's reagent K 71% residual activity at 50 mM Bacillus licheniformis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
Valeraldehyde with NAD+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C Bacillus licheniformis
0.34
-
Valeraldehyde with NADP+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C Bacillus licheniformis
0.46
-
propionaldehyde with NAD+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C Bacillus licheniformis
0.65
-
propionaldehyde with NADP+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C Bacillus licheniformis
2.95
-
Butyraldehyde with NAD+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C Bacillus licheniformis
4.93
-
Butyraldehyde with NADP+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C Bacillus licheniformis
9.87
-
Isovaleraldehyde with NAD+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C Bacillus licheniformis
10.78
-
Isovaleraldehyde with NADP+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C Bacillus licheniformis
18.89
-
acetaldehyde with NAD+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C Bacillus licheniformis
393.4
-
acetaldehyde with NADP+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C Bacillus licheniformis

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the enzyme does not require metal ions for its enzymatic reaction Bacillus licheniformis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52682
-
4 * 52682, calculated from amino acid sequence Bacillus licheniformis
53000
-
4 * 53000, calculated from amino acid sequence Bacillus licheniformis
220000
-
gel filtration Bacillus licheniformis

Organism

Organism UniProt Comment Textmining
Bacillus licheniformis
-
-
-
Bacillus licheniformis ATCC 14580
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-NTA resin column chromatography Bacillus licheniformis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + NAD+
-
Bacillus licheniformis acetate + NADH + H+
-
?
acetaldehyde + NADP+
-
Bacillus licheniformis acetate + NADPH + H+
-
?
butyraldehyde + NAD+
-
Bacillus licheniformis butyrate + NADH + H+
-
?
butyraldehyde + NADP+
-
Bacillus licheniformis butyrate + NADPH + H+
-
?
isovaleraldehyde + NAD+
-
Bacillus licheniformis isovalerate + NADH + H+
-
?
isovaleraldehyde + NADP+
-
Bacillus licheniformis isovalerate + NADPH + H+
-
?
isovaleraldehyde + NADP+
-
Bacillus licheniformis ATCC 14580 isovalerate + NADPH + H+
-
?
additional information no activity with formaldehyde and benzaldehyde Bacillus licheniformis ?
-
?
additional information no activity with formaldehyde and benzaldehyde Bacillus licheniformis ATCC 14580 ?
-
?
n-propanal + NADP+
-
Bacillus licheniformis propionate + NADPH + H+
-
?
propionaldehyde + NAD+ + H2O best substrate combination Bacillus licheniformis propionate + NADH + H+
-
?
propionaldehyde + NAD+ + H2O best substrate combination Bacillus licheniformis ATCC 14580 propionate + NADH + H+
-
?
propionaldehyde + NADP+ + H2O
-
Bacillus licheniformis propionate + NADPH + H+
-
?
propionaldehyde + NADP+ + H2O
-
Bacillus licheniformis ATCC 14580 propionate + NADPH + H+
-
?
valeraldehyde + NAD+
-
Bacillus licheniformis valerate + NADH + H+
-
?
valeraldehyde + NADP+
-
Bacillus licheniformis valerate + NADPH + H+
-
?
valeraldehyde + NADP+
-
Bacillus licheniformis ATCC 14580 valerate + NADPH + H+
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 52682, calculated from amino acid sequence Bacillus licheniformis
homotetramer 4 * 53000, calculated from amino acid sequence Bacillus licheniformis

Synonyms

Synonyms Comment Organism
aldehyde: NAD(P)+ oxidoreductase
-
Bacillus licheniformis
ALDH
-
Bacillus licheniformis
NAD(P)+-dependent aldehyde dehydrogenase
-
Bacillus licheniformis
ybcD
-
Bacillus licheniformis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Bacillus licheniformis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 40 the enzyme appears to be stable at 20°C, but becomes very unstable at a temperature above 37°C, most likely due to thermal denaturation Bacillus licheniformis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.91
-
Isovaleraldehyde with NADP+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C Bacillus licheniformis
1.11
-
acetaldehyde with NADP+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C Bacillus licheniformis
3.58
-
Isovaleraldehyde with NAD+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C Bacillus licheniformis
4.46
-
acetaldehyde with NAD+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C Bacillus licheniformis
11.5
-
Butyraldehyde with NADP+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C Bacillus licheniformis
13.36
-
propionaldehyde with NADP+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C Bacillus licheniformis
19.41
-
Valeraldehyde with NADP+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C Bacillus licheniformis
30.99
-
Valeraldehyde with NAD+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C Bacillus licheniformis
32.8
-
Butyraldehyde with NAD+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C Bacillus licheniformis
49.38
-
propionaldehyde with NAD+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C Bacillus licheniformis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Bacillus licheniformis

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 6 pH 6.0 attenuates more than 39% of enzymatic activity. At pH 5.0, 3% of the activity remains. The enzymatic activity is completely abolished at pH values of or below 4.0 Bacillus licheniformis

Cofactor

Cofactor Comment Organism Structure
NAD+ preferred cofactor Bacillus licheniformis
NADP+
-
Bacillus licheniformis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.003
-
acetaldehyde with NADP+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C Bacillus licheniformis
0.09
-
Isovaleraldehyde with NADP+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C Bacillus licheniformis
0.24
-
acetaldehyde with NAD+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C Bacillus licheniformis
0.36
-
Isovaleraldehyde with NAD+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C Bacillus licheniformis
2.39
-
Butyraldehyde with NADP+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C Bacillus licheniformis
11.89
-
Butyraldehyde with NAD+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C Bacillus licheniformis
20.67
-
propionaldehyde with NADP+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C Bacillus licheniformis
56.66
-
Valeraldehyde with NADP+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C Bacillus licheniformis
107.5
-
propionaldehyde with NAD+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C Bacillus licheniformis
257
-
Valeraldehyde with NAD+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C Bacillus licheniformis