Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 246% activity at 2 mM | Bacillus licheniformis | |
dithiothreitol | 276% activity at 2 mM | Bacillus licheniformis |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli M15(pREP4) cells | Bacillus licheniformis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,2-Cyclohexanedione | 33% residual activity at 2.5 mM | Bacillus licheniformis | |
2,4-Dinitro-1-fluorobenzene | 11% residual activity at 2.5 mM | Bacillus licheniformis | |
Co2+ | 89% residual activity at 1 mM | Bacillus licheniformis | |
Cu2+ | 20% residual activity at 1 mM | Bacillus licheniformis | |
diethyl dicarbonate | 2% residual activity at 2.5 mM | Bacillus licheniformis | |
ethyl acetimidate | 71% residual activity at 250 mM | Bacillus licheniformis | |
Fe2+ | 88% residual activity at 1 mM | Bacillus licheniformis | |
Hg2+ | complete inhibition at 0.25 mM | Bacillus licheniformis | |
Mg2+ | 89% residual activity at 1 mM | Bacillus licheniformis | |
Mn2+ | 89% residual activity at 1 mM | Bacillus licheniformis | |
additional information | not inhibited by EDTA | Bacillus licheniformis | |
N-Acetylimidazole | 75% residual activity at 2.5 mM | Bacillus licheniformis | |
N-bromosuccinimide | 66% residual activity at 0.5 mM | Bacillus licheniformis | |
phenylmethylsulfonyl fluoride | 73% residual activity at 2.5 mM | Bacillus licheniformis | |
Woodward's reagent K | 71% residual activity at 50 mM | Bacillus licheniformis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
Valeraldehyde | with NAD+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C | Bacillus licheniformis | |
0.34 | - |
Valeraldehyde | with NADP+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C | Bacillus licheniformis | |
0.46 | - |
propionaldehyde | with NAD+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C | Bacillus licheniformis | |
0.65 | - |
propionaldehyde | with NADP+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C | Bacillus licheniformis | |
2.95 | - |
Butyraldehyde | with NAD+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C | Bacillus licheniformis | |
4.93 | - |
Butyraldehyde | with NADP+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C | Bacillus licheniformis | |
9.87 | - |
Isovaleraldehyde | with NAD+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C | Bacillus licheniformis | |
10.78 | - |
Isovaleraldehyde | with NADP+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C | Bacillus licheniformis | |
18.89 | - |
acetaldehyde | with NAD+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C | Bacillus licheniformis | |
393.4 | - |
acetaldehyde | with NADP+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C | Bacillus licheniformis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme does not require metal ions for its enzymatic reaction | Bacillus licheniformis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52682 | - |
4 * 52682, calculated from amino acid sequence | Bacillus licheniformis |
53000 | - |
4 * 53000, calculated from amino acid sequence | Bacillus licheniformis |
220000 | - |
gel filtration | Bacillus licheniformis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus licheniformis | - |
- |
- |
Bacillus licheniformis ATCC 14580 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-NTA resin column chromatography | Bacillus licheniformis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetaldehyde + NAD+ | - |
Bacillus licheniformis | acetate + NADH + H+ | - |
? | |
acetaldehyde + NADP+ | - |
Bacillus licheniformis | acetate + NADPH + H+ | - |
? | |
butyraldehyde + NAD+ | - |
Bacillus licheniformis | butyrate + NADH + H+ | - |
? | |
butyraldehyde + NADP+ | - |
Bacillus licheniformis | butyrate + NADPH + H+ | - |
? | |
isovaleraldehyde + NAD+ | - |
Bacillus licheniformis | isovalerate + NADH + H+ | - |
? | |
isovaleraldehyde + NADP+ | - |
Bacillus licheniformis | isovalerate + NADPH + H+ | - |
? | |
isovaleraldehyde + NADP+ | - |
Bacillus licheniformis ATCC 14580 | isovalerate + NADPH + H+ | - |
? | |
additional information | no activity with formaldehyde and benzaldehyde | Bacillus licheniformis | ? | - |
? | |
additional information | no activity with formaldehyde and benzaldehyde | Bacillus licheniformis ATCC 14580 | ? | - |
? | |
n-propanal + NADP+ | - |
Bacillus licheniformis | propionate + NADPH + H+ | - |
? | |
propionaldehyde + NAD+ + H2O | best substrate combination | Bacillus licheniformis | propionate + NADH + H+ | - |
? | |
propionaldehyde + NAD+ + H2O | best substrate combination | Bacillus licheniformis ATCC 14580 | propionate + NADH + H+ | - |
? | |
propionaldehyde + NADP+ + H2O | - |
Bacillus licheniformis | propionate + NADPH + H+ | - |
? | |
propionaldehyde + NADP+ + H2O | - |
Bacillus licheniformis ATCC 14580 | propionate + NADPH + H+ | - |
? | |
valeraldehyde + NAD+ | - |
Bacillus licheniformis | valerate + NADH + H+ | - |
? | |
valeraldehyde + NADP+ | - |
Bacillus licheniformis | valerate + NADPH + H+ | - |
? | |
valeraldehyde + NADP+ | - |
Bacillus licheniformis ATCC 14580 | valerate + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 52682, calculated from amino acid sequence | Bacillus licheniformis |
homotetramer | 4 * 53000, calculated from amino acid sequence | Bacillus licheniformis |
Synonyms | Comment | Organism |
---|---|---|
aldehyde: NAD(P)+ oxidoreductase | - |
Bacillus licheniformis |
ALDH | - |
Bacillus licheniformis |
NAD(P)+-dependent aldehyde dehydrogenase | - |
Bacillus licheniformis |
ybcD | - |
Bacillus licheniformis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Bacillus licheniformis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 40 | the enzyme appears to be stable at 20°C, but becomes very unstable at a temperature above 37°C, most likely due to thermal denaturation | Bacillus licheniformis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.91 | - |
Isovaleraldehyde | with NADP+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C | Bacillus licheniformis | |
1.11 | - |
acetaldehyde | with NADP+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C | Bacillus licheniformis | |
3.58 | - |
Isovaleraldehyde | with NAD+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C | Bacillus licheniformis | |
4.46 | - |
acetaldehyde | with NAD+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C | Bacillus licheniformis | |
11.5 | - |
Butyraldehyde | with NADP+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C | Bacillus licheniformis | |
13.36 | - |
propionaldehyde | with NADP+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C | Bacillus licheniformis | |
19.41 | - |
Valeraldehyde | with NADP+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C | Bacillus licheniformis | |
30.99 | - |
Valeraldehyde | with NAD+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C | Bacillus licheniformis | |
32.8 | - |
Butyraldehyde | with NAD+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C | Bacillus licheniformis | |
49.38 | - |
propionaldehyde | with NAD+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C | Bacillus licheniformis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Bacillus licheniformis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 6 | pH 6.0 attenuates more than 39% of enzymatic activity. At pH 5.0, 3% of the activity remains. The enzymatic activity is completely abolished at pH values of or below 4.0 | Bacillus licheniformis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | preferred cofactor | Bacillus licheniformis | |
NADP+ | - |
Bacillus licheniformis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
acetaldehyde | with NADP+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C | Bacillus licheniformis | |
0.09 | - |
Isovaleraldehyde | with NADP+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C | Bacillus licheniformis | |
0.24 | - |
acetaldehyde | with NAD+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C | Bacillus licheniformis | |
0.36 | - |
Isovaleraldehyde | with NAD+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C | Bacillus licheniformis | |
2.39 | - |
Butyraldehyde | with NADP+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C | Bacillus licheniformis | |
11.89 | - |
Butyraldehyde | with NAD+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C | Bacillus licheniformis | |
20.67 | - |
propionaldehyde | with NADP+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C | Bacillus licheniformis | |
56.66 | - |
Valeraldehyde | with NADP+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C | Bacillus licheniformis | |
107.5 | - |
propionaldehyde | with NAD+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C | Bacillus licheniformis | |
257 | - |
Valeraldehyde | with NAD+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C | Bacillus licheniformis |