Cloned (Comment) | Organism |
---|---|
expressed as a hexa-histidine-tagged fusion in Escherichia coli BL21 Star (DE3) | Paraburkholderia xenovorans LB400 |
Crystallization (Comment) | Organism |
---|---|
ALDHC in complex with NADPH bound in the cofactor binding pocket and an ordered fragment of a polyethylene glycol molecule bound in the substrate tunnel, to 1.6 A resolution. Belongs to space group P1. The ALDHC monomer comprises three distinct domains, an N-terminal cofactor (NAD/P+)-binding domain, a catalytic domain, and an oligomerization domain | Paraburkholderia xenovorans LB400 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
NADP+ | - |
Paraburkholderia xenovorans LB400 | |
0.042 | - |
Heptanal | - |
Paraburkholderia xenovorans LB400 | |
0.3 | - |
Valeraldehyde | - |
Paraburkholderia xenovorans LB400 | |
0.501 | - |
NAD+ | - |
Paraburkholderia xenovorans LB400 | |
1.21 | - |
propionaldehyde | - |
Paraburkholderia xenovorans LB400 | |
1.66 | - |
Isovaleraldehyde | - |
Paraburkholderia xenovorans LB400 | |
1.9 | - |
formaldehyde | - |
Paraburkholderia xenovorans LB400 | |
4.15 | - |
benzaldehyde | - |
Paraburkholderia xenovorans LB400 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
110000 | - |
gel filtration | Paraburkholderia xenovorans LB400 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paraburkholderia xenovorans | Q13WK4 | - |
- |
Paraburkholderia xenovorans LB400 | Q13WK4 | - |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity by nickel affinity chromatography and gel filtration | Paraburkholderia xenovorans LB400 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzaldehyde + NADP+ + H2O | - |
Paraburkholderia xenovorans LB400 | benzoate + NADPH + H+ | - |
? | |
formaldehyde + NADP+ + H2O | - |
Paraburkholderia xenovorans LB400 | formate + NADPH + H+ | - |
? | |
heptanal + NADP+ + H2O | - |
Paraburkholderia xenovorans LB400 | heptanoate + NADPH + H+ | - |
? | |
isovaleraldehyde + NADP+ + H2O | - |
Paraburkholderia xenovorans LB400 | isovalerate + NADPH + H+ | - |
? | |
additional information | ALDHC is preferentially active towards linear medium-chain to long-chain aldehydes as compared to branched-chain, short-chain or aromatic aldehydes. Reorientation of an extended loop (Asn478-Pro490) is responsible for the constricted structure of the substrate tunnel, with the side chain of Asn478 imposing steric restrictions on branched-chain and aromatic aldehydes. A key glycine (Gly104) positioned at the mouth of the tunnel allows for maximum tunnel depth required to bind the linear medium to long aliphatic chain of the native substrate | Paraburkholderia xenovorans LB400 | ? | - |
? | |
propionaldehyde + NAD+ + H2O | - |
Paraburkholderia xenovorans LB400 | propionate + NADH + H+ | - |
r | |
propionaldehyde + NAD+ + H2O | - |
Paraburkholderia xenovorans | propionate + NADH + H+ | - |
? | |
propionaldehyde + NADP+ + H2O | - |
Paraburkholderia xenovorans LB400 | propionate + NADPH + H+ | - |
? | |
valeraldehyde + NADP+ + H2O | - |
Paraburkholderia xenovorans LB400 | valerate + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | gel filtration | Paraburkholderia xenovorans LB400 |
Synonyms | Comment | Organism |
---|---|---|
aldehyde dehydrogenase | - |
Paraburkholderia xenovorans LB400 |
ALDHC | - |
Paraburkholderia xenovorans LB400 |
chromosomally encoded box pathway ALDH | - |
Paraburkholderia xenovorans LB400 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Paraburkholderia xenovorans LB400 | |
NADP+ | significant preference for NADP+. Cofactor selectivity is governed by a complex network of hydrogen bonds between the oxygen atoms of the 2'-phosphoryl moiety of NADP+ and a threonine/lysine pair on ALDHC | Paraburkholderia xenovorans LB400 |