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Literature summary for 1.2.1.5 extracted from

  • Evces, S.; Lindahl, R.
    Characterization of rat cornea aldehyde dehydrogenase (1989), Arch. Biochem. Biophys., 274, 518-524.
    View publication on PubMed
Search for more literature for 1.2.1.5

Inhibitors

Inhibitors Comment Organism Structure
Disulfiram 0.025 mM, 99% inhibition of activity with NADP+, 80% inhibition of activity with activity with NAD+ Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
 NAD+
-
 Rattus norvegicus
0.2
-
 benzaldehyde enzyme from cornea, reaction with NADP+ Rattus norvegicus
0.3
-
 benzaldehyde enzyme from HTC hepatoma cells, reaction with NADP+ Rattus norvegicus
0.42
-
 NADP+
-
 Rattus norvegicus
2.7
-
 propanal enzyme from HTC hepatoma cells, reaction with NAD+ Rattus norvegicus
3
-
 NAD+ enzyme from cornea, reaction with propanal Rattus norvegicus
5.7
-
 propanal enzyme from cornea, reaction with NAD+ Rattus norvegicus
35
-
 NAD+ enzyme from HTC hepatoma cells, reaction with propanal Rattus norvegicus
240
-
 NADP+ enzyme from HTC hepatoma cells, reaction with benzaldehyde Rattus norvegicus
260
-
 NADP+ enzyme from cornea, reaction with benzaldehyde Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51000
-
 2 * 51000, SDS-PAGE Rattus norvegicus
100000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Rattus norvegicus the enzyme plays a role in oxidation of a variety of aldehydes generated by lipid metabolism ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
cornea
-
 Rattus norvegicus
-
HTC cell
-
 Rattus norvegicus
-
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
110.5
-
 liver enzyme Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,5-dihydroxybenzaldehyde + NADP+ + H2O
-
 Rattus norvegicus 2,5-dihydroxybenzoate + NADPH + H+
-
 ?
2-methylbutyraldehyde + NAD+ + H2O
-
 Rattus norvegicus 2-methylbutyrate + NADH + H+
-
 ?
3-hydroxybenzaldehyde + NADP+ + H2O
-
 Rattus norvegicus 3-hydroxybenzoate + NADPH + H+
-
 ?
4-carboxybenzaldehyde + NADP+ + H2O
-
 Rattus norvegicus 4-carboxybenzoate + NADPH + H+
-
 ?
4-chlorobenzaldehyde + NADP+ + H2O
-
 Rattus norvegicus 4-chlorobenzoate + NADPH + H+
-
 ?
4-nitrobenzaldehyde + NAD+ + H2O
-
 Rattus norvegicus 4-nitrobenzoate + NADH + H+
-
 ?
acetaldehyde + NADP+ + H2O
-
 Rattus norvegicus acetate + NADPH + H+
-
 ?
benzaldehyde + NADP+
-
 Rattus norvegicus benzoate + NADPH
-
 ?
dodecanal + NADP+ + H2O
-
 Rattus norvegicus dodecanoate + NADPH + H+
-
 ?
hexanal + NAD+ + H2O
-
 Rattus norvegicus hexanoate + NADH + H+
-
 ?
isobutanal + NAD+ + H2O
-
 Rattus norvegicus isobutyrate + NADH + H+
-
 ?
additional information the enzyme plays a role in oxidation of a variety of aldehydes generated by lipid metabolism Rattus norvegicus ?
-
 ?
nonanal + NADP+ + H2O
-
 Rattus norvegicus nonanoate + NADPH + H+
-
 ?
octanal + NAD+ + H2O
-
 Rattus norvegicus octanoate + NADH + H+
-
 ?
phenylacetaldehyde + NADP+ + H2O
-
 Rattus norvegicus phenylacetate + NADPH + H+
-
 ?
propanal + NAD+ + H2O
-
 Rattus norvegicus propionate + NADH + H+
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 51000, SDS-PAGE Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 4 min, 98% loss of activity Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 activity with NADP+ Rattus norvegicus
10
-
 activity with NAD+, broad plateau from pH 5 to 8 followed by a sharp increase in activity to pH 10 Rattus norvegicus