Information on EC 3.4.21.1 - chymotrypsin

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
3.4.21.1
-
RECOMMENDED NAME
GeneOntology No.
chymotrypsin
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Preferential cleavage: Tyr-/-, Trp-/-, Phe-/-, Leu-/-
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
endopeptidase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9004-07-3
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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-
Manually annotated by BRENDA team
Diabrotica virgifera virgifera
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-
Manually annotated by BRENDA team
2 enzyme forms: A and B
-
-
Manually annotated by BRENDA team
2 enzyme forms: LCTa and LCTb
-
-
Manually annotated by BRENDA team
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-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
isoenzyme CTRA-1
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-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Sardinops sagax caerulea, Monterey sardine
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-
Manually annotated by BRENDA team
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-
Manually annotated by BRENDA team
European catfish
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-
Manually annotated by BRENDA team
fire ant
Uniprot
Manually annotated by BRENDA team
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-
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
synthetic construct
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-[2-(2-[[4-([1-benzyl-2-[(4-nitrophenyl)amino]-2-oxoethyl]amino)-4-oxobutanoyl]amino]ethoxy)ethoxy]ethanaminium + H2O
?
show the reaction diagram
-
-
-
-
?
4-([1-benzyl-2-[(4-nitrophenyl)amino]-2-oxoethyl]amino)-4-oxobutanoic acid + H2O
?
show the reaction diagram
-
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl benzoate + H2O
4-nitrophenol + benzoate
show the reaction diagram
-
-
-
-
?
4-[2-(2-[[4-([1-benzyl-2-[(4-nitrophenyl)amino]-2-oxoethyl]amino)-4-oxobutanoyl]amino]ethoxy)ethoxy]butanoate + H2O
?
show the reaction diagram
-
-
-
-
?
acetyl-L-Tyr ethyl ester + H2O
acetyl-L-Tyr + ethanol
show the reaction diagram
-
-
-
-
?
acetyl-Leu methyl ester + H2O
acetyl-Leu + methanol
show the reaction diagram
-
-
-
-
?
acetyl-Phe ethyl ester + H2O
acetyl-Phe + ethanol
show the reaction diagram
-
-
-
-
?
acetyl-Trp ethyl ester + H2O
acetyl-Trp + ethanol
show the reaction diagram
-
-
-
-
?
acetyl-Tyr ethyl ester + H2O
acetyl-Tyr + ethanol
show the reaction diagram
-
-
-
-
?
Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
-
-
-
-
?
azocasein + H2O
?
show the reaction diagram
-
-
-
-
?
azocasein + H2O
fragments of azocasein
show the reaction diagram
benzoyl-4-guanidinophenyl ester + H2O
benzoyl + 4-guanidinophenol
show the reaction diagram
-
-
-
?
benzoyl-D-Ala-4-guanidinophenyl ester + H2O
benzoyl-D-Ala + 4-guanidinophenol
show the reaction diagram
-
5% of activity with N-benzoyl-L-Ala-4-guanidinophenyl ester
-
?
benzoyl-D-Leu-4-guanidinophenyl ester + H2O
benzoyl-D-Leu + 4-guanidinophenol
show the reaction diagram
-
-
-
?
benzoyl-D-Phe-4-guanidinophenyl ester + H2O
benzoyl-D-Phe + 4-guanidinophenol
show the reaction diagram
-
-
-
?
benzoyl-Gly-4-guanidinophenyl ester + H2O
benzoyl-Gly + 4-guanidinophenol
show the reaction diagram
-
-
-
?
benzoyl-L-Ala-4-guanidinophenyl ester + H2O
benzoyl-L-Ala + 4-guanidinophenol
show the reaction diagram
-
-
-
?
benzoyl-L-tyrosine ethyl ester + H2O
?
show the reaction diagram
-
-
-
-
?
benzoyl-L-tyrosine ethyl ester + H2O
benzoyl-L-tyrosine + ethanol
show the reaction diagram
-
-
-
-
?
benzoyl-Tyr-p-nitroanilide + H2O
benzoyl-tyrosine + p-nitroaniline
show the reaction diagram
-
-
-
?
benzoyl-tyrosine p-nitroanilide + H2O
benzoyl-tyrosine + p-nitroaniline
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-L-Ala-L-Ala-L-Leu-4-nitroanilide + H2O
benzyloxycarbonyl-L-Ala-L-Ala-L-L-Leu + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
beta-lactoglobulin A + H2O
?
show the reaction diagram
-
-
chymotrypsin hydrolyzes beta-lactoglobulin A after Tyr, Trp, Phe, Met, and Leu, peptides corresponding to the cleavage of bonds with Glu, Gln, and Lys at the N-terminal side are also found
-
?
BH3 interacting domain death agonist + H2O
?
show the reaction diagram
-
-
-
?
bovine chymotrypsinogen + H2O
?
show the reaction diagram
-
-
-
-
?
Bovine serum albumin + H2O
?
show the reaction diagram
bovine serum albumine + H2O
?
show the reaction diagram
-
-
-
-
?
calcineurin + H2O
?
show the reaction diagram
-
-
chymotrypsin cleaves calcineurin into a 47-kDa fragment and a 13-kDa fragment, the cleavage activates the phosphatase activity of calcineurin in a Ca2+/CaM-independent manner
-
?
casein + H2O
?
show the reaction diagram
-
-
-
-
?
casein + H2O
fragments of casein
show the reaction diagram
cationic trypsinogen + H2O
?
show the reaction diagram
-
-
-
-
?
cheese whey protein + H2O
?
show the reaction diagram
-
-
-
-
r
deltamethrin + H2O
3-phenoxybenzaldehyde + (1R,3R)-3-(2,2-dibromoethenyl)-2,2-dimethylcyclopropanecarboxylate
show the reaction diagram
-
-
-
-
?
Gelatin + H2O
?
show the reaction diagram
-
chymotrypsin A is active in degrading gelatin, while chymotrypsin B does not reveal activity against gelatin
-
-
?
hippuryl-L-phenylalanine + H2O
hippuric acid + L-phenylalanine
show the reaction diagram
-
1 mM in 25mM Tris-HCl buffer containing 0.5M NaCl
-
-
?
L-Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O
L-Ala-Ala-Phe + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
L-Tyr ethyl ester + H2O
L-Tyr + ethanol
show the reaction diagram
m-calpain + H2O
fragments of m-calpain
show the reaction diagram
-
-
-
?
mCry3A + H2O
mCry3A fragments
show the reaction diagram
-
Bacillus thuringiensis toxin
chymotrypsin cleaves mCry3A into stable 55000 and 49000 Da fragments, as well as smaller polypeptides having molecular masses of 6000 to 11000 Da
-
?
milk-acetate mixture + H2O
?
show the reaction diagram
-
-
-
-
?
mu-calpain + H2O
fragments of mu-calpain
show the reaction diagram
-
-
-
?
N-acetyl-DL-Phe methyl ester + H2O
N-acetyl-L-Phe + methanol
show the reaction diagram
-
alpha-chymotrypsin immobilized to mesoporous silica
enatiomeric excess greater than 99%
?
N-acetyl-DL-Phe-beta-naphthylester + H2O
N-acetyl-DL-Phe + beta-naphthol
show the reaction diagram
-
-
-
?
N-acetyl-DL-phenylglycine methyl ester + H2O
N-acetyl-L-phenylglycine + methanol
show the reaction diagram
-
alpha-chymotrypsin immobilized to mesoporous silica
enatiomeric excess greater than 99%
?
N-acetyl-L-leucine methyl ester + H2O
N-acetyl-L-leucine + methanol
show the reaction diagram
N-acetyl-L-Phe methyl ester + H2O
N-acetyl-L-Phe + methanol
show the reaction diagram
-
alpha-chymotrypsin immobilized to mesoporous silica
-
?
N-acetyl-L-Phe-Ala-Thr-Pal-5-amido-2-nitrobenzoic acid + H2O
N-acetyl-L-Phe-Ala-Thr-Pal + 5-amino-2-nitrobenzoic acid
show the reaction diagram
-
-
-
-
?
N-acetyl-L-Phe-Ala-Thr-Phe-(p-CN)-5-amido-2-nitrobenzoic acid + H2O
N-acetyl-L-Phe-Ala-Thr-Phe-(p-CN) + 5-amino-2-nitrobenzoic acid
show the reaction diagram
-
-
-
-
?
N-acetyl-L-Phe-Ala-Thr-Phe-(p-COOH)-5-amido-2-nitrobenzoic acid + H2O
N-acetyl-L-Phe-Ala-Thr-Phe-(p-COOH) + 5-amino-2-nitrobenzoic acid
show the reaction diagram
-
-
-
-
?
N-acetyl-L-Phe-Ala-Thr-Phe-(p-COOMe)-5-amido-2-nitrobenzoic acid + H2O
N-acetyl-L-Phe-Ala-Thr-Phe-(p-COOMe) + 5-amino-2-nitrobenzoic acid
show the reaction diagram
-
-
-
-
?
N-acetyl-L-Phe-Ala-Thr-Phe-(p-guanidine)-5-amido-2-nitrobenzoic acid + H2O
N-acetyl-L-Phe-Ala-Thr-Phe-(p-guanidine) + 5-amino-2-nitrobenzoic acid
show the reaction diagram
-
-
-
-
?
N-acetyl-L-Phe-Ala-Thr-Phe-(p-NH2)-5-amido-2-nitrobenzoic acid + H2O
N-acetyl-L-Phe-Ala-Thr-Phe-(p-NH2) + 5-amino-2-nitrobenzoic acid
show the reaction diagram
-
-
-
-
?
N-acetyl-L-Phe-Ala-Thr-Phe-(p-NO2)-5-amido-2-nitrobenzoic acid + H2O
N-acetyl-L-Phe-Ala-Thr-Phe-(p-NO2) + 5-amino-2-nitrobenzoic acid
show the reaction diagram
-
-
-
-
?
N-acetyl-L-Phe-Ala-Thr-Phe-5-amido-2-nitrobenzoic acid + H2O
N-acetyl-L-Phe-Ala-Thr-Phe + 5-amino-2-nitrobenzoic acid
show the reaction diagram
-
-
-
-
?
N-acetyl-L-Phe-Ala-Thr-Tyr-5-amido-2-nitrobenzoic acid + H2O
N-acetyl-L-Phe-Ala-Thr-Tyr + 5-amino-2-nitrobenzoic acid
show the reaction diagram
-
-
-
-
?
N-acetyl-L-phenylalanine ethyl ester + glycinamide
N-Ac-Phe-Gly-NH2 + ethanol
show the reaction diagram
-
-
-
-
?
N-acetyl-L-phenylalanine ethyl ester + H2O
N-acetyl-L-phenylalanine + ethanol
show the reaction diagram
N-acetyl-L-tryptophan ethyl ester + H2O
N-acetyl-L-tryptophan + ethanol
show the reaction diagram
N-acetyl-L-Tyr ethyl ester + H2O
N-acetyl-L-tyrosine + ethanol
show the reaction diagram
-
-
-
?
N-acetyl-L-Tyr-ethyl ester + H2O
N-acetyl-L-Tyr + ethanol
show the reaction diagram
-
-
-
?
N-acetyl-L-Tyr-p-nitroanilide + H2O
N-acetyl-L-Tyr + p-nitroaniline
show the reaction diagram
-
-
-
?
N-acetyl-L-tyrosine ethyl ester + H2O
N-acetyl-L-tyrosine
show the reaction diagram
-
-
-
?
N-acetyl-L-tyrosine ethyl ester + H2O
N-acetyl-L-tyrosine + ethanol
show the reaction diagram
N-alpha-acetyl-L-tyrosine ethyl ester + H2O
N-alpha-acetyl-L-tyrosine + ethanol
show the reaction diagram
-
-
-
?
N-alpha-benzoyl-DL-arginine-p-nitroanilide + H2O
? + p-nitroaniline
show the reaction diagram
N-alpha-benzoyl-L-tyrosine ethyl ester + H2O
?
show the reaction diagram
-
-
-
-
?
N-alpha-benzoyl-L-tyrosyl ethyl ester + H2O
?
show the reaction diagram
-
-
-
-
?
N-benzoyl-L-arginine ethyl ester + H2O
N-benzoyl-L-arginine + ethanol
show the reaction diagram
-
0.5 mM in 50 mM phosphate buffer, pH 7.6
-
-
?
N-benzoyl-L-Tyr ethyl ester + H2O
N-benzoyl-L-Tyr + ethanol
show the reaction diagram
N-benzoyl-L-Tyr p-nitroanilide + H2O
N-benzoyl-L-tyrosine + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
N-benzoyl-L-Tyr-p-nitroanilide + H2O
N-benzoyl-L-tyrosine + 4-nitroaniline
show the reaction diagram
N-benzoyl-L-Tyr-p-nitroanilide + H2O
N-benzoyl-tyrosine + 4-nitroaniline
show the reaction diagram
-
-
-
?
N-benzoyl-L-tyrosine 4-nitroanilide + H2O
N-benzoyl-L-tyrosine + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
N-benzoyl-L-tyrosine ethyl ester + H2O
?
show the reaction diagram
-
-
-
-
?
N-benzoyl-L-tyrosine ethyl ester + H2O
N-benzoyl-L-tyrosine + ethanol
show the reaction diagram
N-benzoyl-L-tyrosine ethyl ester + methanol
N-benzoyl-L-tyrosine methyl ester + ethanol
show the reaction diagram
-
transesteriferication
-
?
N-benzoyl-L-tyrosine methyl ester + ethanol
N-benzoyl-L-tyrosine ethyl ester + methanol
show the reaction diagram
-
transesteriferication
-
?
N-benzoyl-L-tyrosine methyl ester + H2O
N-benzoyl-L-tyrosine + methanol
show the reaction diagram
-
-
-
?
N-benzoyl-L-tyrosine p-nitroanilide + H2O
N-benzoyl-L-tyrosine + p-nitroaniline
show the reaction diagram
-
-
-
?
N-benzoyl-L-tyrosine-4-nitroanilide + H2O
N-benzoyl-L-tyrosine + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
N-benzoyl-L-tyrosine-p-nitroanilide + H2O
N-benzoyl-L-tyrosine + p-nitroaniline
show the reaction diagram
N-benzoyl-Tyr p-nitroanilide + H2O
N-benzoyl-Tyr + p-nitroaniline
show the reaction diagram
N-benzyloxycarbonyl-L-Phe-Ala-Thr-Phe-(p-NO2)-5-amido-2-nitrobenzoic acid + H2O
N-benzyloxycarbonyl-L-Phe-Ala-Thr-Phe-(p-NO2) + 5-amino-2-nitrobenzoic acid
show the reaction diagram
-
-
-
-
?
N-benzyloxycarbonyl-L-Phe-Ala-Thr-Tyr-5-amido-2-nitrobenzoic acid + H2O
N-benzyloxycarbonyl-L-Phe-Ala-Thr-Tyr + 5-amino-2-nitrobenzoic acid
show the reaction diagram
-
-
-
-
?
N-glutamyl-L-phenylalanine p-nitroanilide + H2O
p-nitroaniline + N-glutamyl-L-phenylalanine
show the reaction diagram
-
-
-
-
?
N-glutaryl-L-Phe-p-nitroanilide + H2O
N-glutaryl-L-Phe + p-nitroaniline
show the reaction diagram
-
-
-
?
N-glutaryl-L-phenylalanine-p-nitroanilide + H2O
N-glutaryl-L-phenylalanine + p-nitroaniline
show the reaction diagram
-
-
-
-
?
N-succinyl-Ala-Ala-Ala p-nitroanilide + H2O
N-succinyl-Ala-Ala-Ala + p-nitroaniline
show the reaction diagram
-
-
-
-
?
N-succinyl-Ala-Ala-Ala-p-nitroanilide + H2O
N-succinyl-Ala-Ala-Ala + p-nitroaniline
show the reaction diagram
-
-
-
-
?
N-succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O
N-succinyl-Ala-Ala-Phe + 7-amino-4-methylcoumarin
show the reaction diagram
N-succinyl-Ala-Ala-Pro-Arg-p-nitroanilide + H2O
N-succinyl-Ala-Ala-Pro-Arg + p-nitroaniline
show the reaction diagram
-
very low activity
-
?
N-succinyl-Ala-Ala-Pro-Leu-4-nitroanilide + H2O
N-succinyl-Ala-Ala-Pro-Leu + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
N-succinyl-Ala-Ala-Pro-Leu-p-nitroanilide + H2O
N-succinyl-Ala-Ala-Pro-Leu + p-nitroaniline
show the reaction diagram
-
-
-
?
N-succinyl-Ala-Ala-Pro-Lys-7-amido-4-methylcoumarin + H2O
N-succinyl-Ala-Ala-Pro-Lys + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
N-succinyl-Ala-Ala-Pro-Lys-p-nitroanilide + H2O
N-succinyl-Ala-Ala-Pro-Lys + p-nitroaniline
show the reaction diagram
-
extremly low activity
-
?
N-succinyl-Ala-Ala-Pro-Met-p-nitroanilide + H2O
N-succinyl-Ala-Ala-Pro-Met + p-nitroaniline
show the reaction diagram
-
-
-
?
N-succinyl-Ala-Ala-Pro-Nle-p-nitroanilide + H2O
N-succinyl-Ala-Ala-Pro-Nle + p-nitroaniline
show the reaction diagram
-
-
-
?
N-succinyl-Ala-Ala-Pro-Nva-p-nitroanilide + H2O
N-succinyl-Ala-Ala-Pro-Nva + p-nitroaniline
show the reaction diagram
-
-
-
?
N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide + H2O
N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O
N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O
N-succinyl-Ala-Ala-Pro-Phe + p-nitroaniline
show the reaction diagram
-
-
-
-
?
N-succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin + H2O
N-succinyl-Ala-Ala-Pro-Phe + 7-amino-4-methylcoumarin
show the reaction diagram
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide + H2O
N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
show the reaction diagram
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide + H2O
N-succinyl-Ala-Ala-Pro-Phe + p-nitroaniline
show the reaction diagram
N-succinyl-Ala-Phe-Ala + H2O
N-succinyl-Ala-Phe + alanine
show the reaction diagram
-
-
-
?
N-succinyl-Ala-Phe-p-nitroanilide + H2O
N-succinyl-Ala-Phe + p-nitroaniline
show the reaction diagram
-
-
-
?
N-succinyl-Ala-Pro-Phe-p-nitroanilide + H2O
N-succinyl-Ala-Pro-Phe + p-nitroaniline
show the reaction diagram
-
-
-
-
?
N-succinyl-L-Ala-Ala-Phe-p-nitroanilide + H2O
N-succinyl-L-Ala-Ala-Phe + p-nitroaniline
show the reaction diagram
-
-
-
-
?
N-succinyl-L-Ala-Ala-Pro-p-nitroanilide + H2O
N-succinyl-L-Ala-Ala-Pro + p-nitroaniline
show the reaction diagram
-
-
-
-
?
N-succinyl-L-Ala-Ala-Pro-Phe-p-nitroanilide + H2O
N-succinyl-L-Ala-Ala-Pro-Phe + p-nitroaniline
show the reaction diagram
-
-
-
-
?
N-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine p-nitroanilide + H2O
p-nitroaniline + N-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine
show the reaction diagram
-
-
-
-
?
N-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine-p-nitroanilide + H2O
p-nitroaniline + N-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine
show the reaction diagram
-
-
-
-
?
N-succinyl-L-Phe-p-nitroanilide + H2O
N-succinyl-L-Phe + p-nitroaniline
show the reaction diagram
N-succinyl-L-phenylalanine p-nitroanilide + H2O
N-succinyl-L-phenylalanine + p-nitroaniline
show the reaction diagram
-
-
-
-
?
N-succinyl-L-phenylalanine-4-nitroanilide + H2O
N-succinyl-L-phenylalanine + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
N-succinyl-L-phenylalanine-p-nitroanilide + H2O
N-succinyl-L-phenylalanine + p-nitroaniline
show the reaction diagram
-
-
-
-
?
N-succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O
N-succinyl-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin + H2O
N-succinyl-Leu-Tyr + 7-amino-4-methylcoumarin
show the reaction diagram
N-succinyl-Phe-7-amido-4-methylcoumarin + H2O
N-succinyl-Phe + 7-amino-4-methylcoumarin
show the reaction diagram
N-succinyl-Phe-p-nitroanilide + H2O
p-nitroaniline + N-succinyl-Phe
show the reaction diagram
-
-
-
-
?
N-[1-benzyl-2-[(4-nitrophenyl)amino]-2-oxoethyl]-N'-[2-[2-(2-hydroxyethoxy)ethoxy]ethyl]butanediamide + H2O
?
show the reaction diagram
-
-
-
-
?
Nalpha-benzyloxycarbonyl-L-Ala p-nitrophenyl ester + H2O
Nalpha-benzyloxycarbonyl-L-Ala + p-nitrophenol
show the reaction diagram
-
-
-
-
?
Nalpha-benzyloxycarbonyl-L-Leu p-nitrophenyl ester + H2O
Nalpha-benzyloxycarbonyl-L-Leu + p-nitrophenol
show the reaction diagram
-
-
-
-
?
Nalpha-benzyloxycarbonyl-L-Lys p-nitrophenyl ester + H2O
Nalpha-benzyloxycarbonyl-L-Lys + p-nitrophenol
show the reaction diagram
-
-
-
-
?
Nalpha-benzyloxycarbonyl-L-Tyr p-nitrophenyl ester + H2O
Nalpha-benzyloxycarbonyl-L-Tyr + p-nitrophenol
show the reaction diagram
-
-
-
-
?
Nalpha-tosyl-L-Tyr benzyl ester + H2O
Nalpha-tosyl-L-Tyr + phenol
show the reaction diagram
-
-
-
-
?
native collagen + H2O
?
show the reaction diagram
-
-
-
?
p-nitrophenyl acetate + H2O
p-nitrophenol + acetate
show the reaction diagram
-
-
-
-
?
Protein + H2O
?
show the reaction diagram
rat chymotrypsinogen + H2O
?
show the reaction diagram
-
-
-
-
?
SPPNA + H2O
?
show the reaction diagram
-
specific substrate for chymotrypsin
-
-
?
Suc-Ala-Ala-Pro-Phe-4-nitroanilide + H2O
Suc-Ala-Ala-Pro-Phe + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
succinimidyl-Ala-Ala-Pro-Phe-p-nitroanilide + H2O
succinimidyl-Ala-Ala-Pro-Phe + p-nitroaniline
show the reaction diagram
-
-
-
?
succinyl-Ala-Ala-Pro-Leu-p-nitroanilide + H2O
succinyl-Ala-Ala-Pro-Leu + p-nitroaniline
show the reaction diagram
42% of activity with succinyl-Ala-Ala-Pro-Phe-p-nitroanilide
-
?
succinyl-Ala-Ala-Pro-Met-p-nitroanilide + H2O
succinyl-Ala-Ala-Pro-Met + p-nitroaniline
show the reaction diagram
66% of activity with succinyl-Ala-Ala-Pro-Phe-p-nitroanilide
-
?
succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin + H2O
succinyl-Ala-Ala-Pro-Phe + 7-amino-4-methylcoumarin
show the reaction diagram
succinyl-Ala-Ala-Pro-Phe-p-nitroanilide + H2O
?
show the reaction diagram
-
57 microM, pH 7.8
-
-
?
succinyl-Ala-Ala-Pro-Phe-p-nitroanilide + H2O
succinyl-Ala-Ala-Pro-Phe + p-nitroaniline
show the reaction diagram
succinyl-Ala-Ala-Pro-Tyr-7-amido-4-methylcoumarin + H2O
succinyl-Ala-Ala-Pro-Tyr + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
succinyl-Gly-Gly-p-nitroanilide + H2O
succinyl-Gly-Gly + p-nitroaniline
show the reaction diagram
synthetic construct
-
-
-
-
?
succinyl-L-Ala-Ala-Pro-L-phenylalanine-p-nitroanilide + H2O
succinyl-L-Ala-Ala-Pro-L-phenylalanine + p-nitroanilide
show the reaction diagram
-
-
-
-
?
succinyl-L-Ala-L-Ala-L-Pro-L-Leu-4-nitroanilide + H2O
succinyl-L-Ala-L-Ala-L-Pro-L-Leu + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide + H2O
succinyl-L-Ala-L-Ala-L-Pro-L-Phe + 4-nitroaniline
show the reaction diagram
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O
succinyl-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin
show the reaction diagram
Trypsinogen + H2O
?
show the reaction diagram
-
-
-
-
?
Z-Phe-Arg-Thr-Tyr-NH-(3-carbamoyl-4-nitrophenol) + H2O
Z-Phe-Arg-Thr-Tyr + 5-amino-2-nitrobenzamide
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
bovine chymotrypsinogen + H2O
?
show the reaction diagram
-
-
-
-
?
casein + H2O
fragments of casein
show the reaction diagram
Protein + H2O
?
show the reaction diagram
rat chymotrypsinogen + H2O
?
show the reaction diagram
-
-
-
-
?
Trypsinogen + H2O
?
show the reaction diagram
-
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
-
5 mM, 120% of initial activity
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(Cbz-alanyl)aminomethyl boronic acid
-
inhibition decreases in the presence of arabinogalactan
(Cbz-phenylalanyl)aminomethyl boronic acid
-
inhibition decreases in the presence of arabinogalctan
(methanethiolato)[2,2'-(thio-kappaS)bis[ethanethiolato-kappaS]] oxorhenium (V)
-
-
(p-methoxyphenylthiolato-S)[2,6-bis[(mercapto-kappaS)methyl]pyridine-kappaN] oxorhenium(V)
-
-
(S)-(1-benzyl-2-thiolethyl)-carbamic acid benzyl ester
-
inhibition of alpha-chymotrypsin in the presence of Zn2+
2,2'-[[(1-[[(benzyloxy)carbonyl]amino]-2-phenylethyl)phosphoryl]bis(oxybenzene-4,1-diyl)]diacetic acid
-
one of the most potent inhibitors of chymotrypsin within phosphonic analogs of phenylalanine
2,2'-[[(1-[[1-(tert-butoxycarbonyl)-L-prolyl]amino]-3-methylbutyl)phosphoryl]bis(oxybenzene-3,1-diyl)]diacetic acid
-
-
2,3-dichlorophenyl-6-(chloromethyl)-2-oxo-2H-1-benzopyran-3-carboxylate
-
-
2,5-dichlorophenyl-6-(chloromethyl)-2-oxo-2H-1-benzopyran-3-carboxylate
-
-
2-hydroxymethyl-1-(2-phenylethanecarbonyl)pyrrole
-
0.0125 mg/ml, 10% inhibition
2-hydroxymethyl-1-(2-phenylethansulfonyl)pyrrole
-
0.0125 mg/ml, 15% inhibition
2-hydroxymethyl-1-(2-phenylethenylsulfonyl)pyrrole
-
0.0125 mg/ml, 15% inhibition
2-hydroxymethyl-1-(N-phthalyl-L-leucine)pyrrole
-
0.0125 mg/ml, 10% inhibition
2-hydroxymethyl-1-(phenylsulfonyl)pyrrole
-
0.053 mM, 20% inhibition
2-mercaptoethanol
-
-
2-nitrophenyl-6-(chloromethyl)-2-oxo-2H-1-benzopyran-3-carboxylate
-
-
2-oxo-2-(1,2,7,8-tetradehydro-5,6-dihydro-4-benzazecin-4(3H)-yl)ethyl N-(tert-butoxycarbonyl)-L-phenylalaninate
-
enediyne-amino acid conjugate, competitive inhibitor
2-oxo-2-(piperidin-1-yl)ethyl N-(tert-butoxycarbonyl)-L-phenylalaninate
-
piperidine amino acid conjugate, exhibits lesser inhibitory activity compared to the enediyne-peptide conjugates
2-oxo-2-[(5Z)-3,4,7,8-tetradehydro-9,10-dihydroazecin-1(2H)-yl]ethyl N-(tert-butoxycarbonyl)-L-phenylalaninate
-
enediyne-amino acid conjugate
3,4-dichloroisocoumarin
complete inhibition at 0.2 mM
3,5-dichlorophenyl-6-(chloromethyl)-2-oxo-2H-1-benzopyran-3-carboxylate
-
-
3-bromophenyl-6-(chloromethyl)-2-oxo-2H-1-benzopyran-3-carboxylate
-
-
3-chloro-5-methoxyphenyl-6-(chloromethyl)-2-oxo-2H-1-benzopyran-3-carboxylate
-
-
3-chlorophenyl-6(acetoxymethyl)-2-oxo-2H-1-benzopyran-3-carboxylate
-
-
3-chlorophenyl-6-methyl-2-oxo-2H-1-benzopyran-3-carboxylate
-
-
3-fluorophenyl-6-(chloromethyl)-2-oxo-2H-1-benzopyran-3-carboxylate
-
-
3-methoxyphenyl-6-(chloromethyl)-2-oxo-2H-1-benzopyran-3-carboxylate
-
-
3-trifluoromethylphenyl-6-(chloromethyl)-2-oxo-2H-1-benzopyran-3-carboxylate
-
-
4-fluorophenylboronic acid
-
-
4-[(4-nitrophenyl)sulfonyl]-1,2,7,8-tetradehydro-3,4,5,6-tetrahydro-4-benzazecine
-
-
5'-demethoxyepiexcelsin
synthetic construct
-
from Commiphora mukul, noncompetitive inhibitor
7-hydroxycoumarin
-
0.78 mM, 50% inhibition
Ac-Phe-Val-Thr-p-amino-L-phenylalanine-CHO
-
-
Ac-Phe-Val-Thr-p-nitro-L-phenylalanine-CHO
-
-
Ac-Phe-Val-Thr-Phe-CHO
-
-
Ac-Phe-Val-Thr-pyridyl-L-alanine-CHO
-
-
Ac-Phe-Val-Thr-Tyr-CHO
-
-
Acacia confusa chymotrypsin inhibitor
-
large, dimeric 70 kDa chymotrypsin inhibitor with a molar ratio of inhibition between the chymotrypsin inhibitor and alpha-chymotrypsin of 2:1, which gives about 100% inhibition. The molar ratio of chymotrypsin inhibitor to chymotrypsin is usually 1:1. Its chymotrypsin inhibitory activity is stable from pH 3-10 and from 0-50C. Exerts antiproliferative activity toward breast cancer MCF-7 cells
-
Acacia plumosa trypsin inhibitor A
-
strong inhibition
-
Acacia plumosa trypsin inhibitor B
-
strong inhibition
-
Acacia plumosa trypsin inhibitor C
-
strong inhibition
-
aceto[2,6-bis[(butylthio-jS)methyl]phenyl-kappaC]-,(SP-4-3)-palladium(II)
-
-
alpha-chymostatin
-
specific inhibitor
alpha1-Antichymotrypsin
-
forms very long-lived, enzymatically inactive 1/1 compexes with its target
-
alpha2-Macroglobulin
-
nonspecific proteinase inhibitor
-
Alzheimer's amyloid beta-protein precursor
-
engineering of inhibitors with altered specificities
-
anti-trypsin-chymotrypsin A
-
highly potent serine protease inhibitor protein from Theromyzon tessulatum
-
anti-trypsin-chymotrypsin B
-
highly potent serine protease inhibitor protein from Theromyzon tessulatum
-
Aprotinin
AVPGSWPW
-
octapeptide
basic pancreatic trypsin inhibitor
Belactosin C
-
irreversible inhibitor
benzamidine
benzyloxycarbonyl-Ala-Ala-Phe-glyoxal
-
-
benzyloxycarbonyl-Ala-Pro-Phe-glyoxal
benzyloxycarbonyl-Phe-glyoxal
-
-
benzyloxycarbonyl-Pro-Phe-glyoxal
-
-
BGTI1
-
concentration 228 nM: 100% inhibition, concentration 456 nM: 97.8% inhibition, concentration 114 nM: 73.3% inhibition, concentration 57 nM: 41.3% inhibition. Inhibitor from Vigna mungo seeds
-
BGTI2
-
concentration 2188 nM: 53.3% inhibition, concentration 1094 nM: 28.9% inhibition, concentration 547 nM: 11.1% inhibition, concentration 273 nM: 6.7% inhibition. Inhibitor from Vigna mungo seeds
-
BGTI3
-
concentration 5000 nM: 36.7% inhibition, concentration 2500 nM: 22.3% inhibition, concentration 1250 nM: 10.0% inhibition, concentration 625 nM: 0.0% inhibition. Inhibitor from Vigna mungo seeds
-
bis(2,3-dimethylphenyl) (1-[[(benzyloxy)carbonyl]amino]-2-phenylethyl)phosphonate
-
one of the most potent inhibitors of chymotrypsin within phosphonic analogs of phenylalanine
bis(2-methylphenyl) (1-[[(benzyloxy)carbonyl]amino]-2-phenylethyl)phosphonate
-
one of the most potent inhibitors of chymotrypsin within phosphonic analogs of phenylalanine
bis(3,4,5-trimethylphenyl) (1-[[(benzyloxy)carbonyl]amino]-2-phenylethyl)phosphonate
-
-
bis(3,4-dimethylphenyl) (1-[[(benzyloxy)carbonyl]amino]-2-phenylethyl)phosphonate
-
-
bis(3-chlorophenyl) (1-[[(benzyloxy)carbonyl]amino]-2-phenylethyl)phosphonate
-
-
bis(4-chlorophenyl) (1-[[(benzyloxy)carbonyl]amino]-2-phenylethyl)phosphonate
-
one of the most potent inhibitors of chymotrypsin within phosphonic analogs of phenylalanine
bis(4-methoxyphenyl) (1-[[(benzyloxy)carbonyl]amino]-2-phenylethyl)phosphonate
-
one of the most potent inhibitors of chymotrypsin within phosphonic analogs of phenylalanine
bis(4-tert-butylphenyl) (1-[[(benzyloxy)carbonyl]amino]-2-phenylethyl)phosphonate
-
one of the most potent inhibitors of chymotrypsin within phosphonic analogs of phenylalanine
bis[4-(propan-2-yl)phenyl] (1-[[(benzyloxy)carbonyl]amino]-2-phenylethyl)phosphonate
-
one of the most potent inhibitors of chymotrypsin within phosphonic analogs of phenylalanine
bis[4-(sulfanylmethyl)phenyl] (1-[[(benzyloxy)carbonyl]amino]-2-phenylethyl)phosphonate
-
-
black-eyed pea trypsin/chymotrypsin inhibitor
BTCI, is able to inhibit trypsin and chymotrypsin simultaneously by forming a stable ternary complex
-
bovine basic panreatic trypsin inhibitor
-
-
-
Bovine pancreatic trypsin inhibitor
-
300fold stronger inhibition of alpha-chymotrypsin in the presence of 3 M NaCl
-
Bowman-Birk inhibitor
-
-
BPTI
-
basic pancreatic trypsin inhibitor, inhibition of Chtr1 in 0.1 M Tris-HCl, pH 7.8. With 0.5 M NaCl no inhibition detected
-
brein 3-O-myristate
-
0.000078 mM, 50% inhibition, noncompetitive inhibition
brein 3-O-palmitate
-
0.000042 mM, 50% inhibition, noncompetitive inhibition
broad bean trypsin-chymotrypsin inhibitor
-
7500 Da peptide isolated from from Vicia faba, almost complete inhibition at a molar ratio of 20
-
Cd2+
-
partially inactivates both chymotrypsin A and B at concentrations of 1 and 5 mM. At room temperature, at 5 mM concentration, 76.8% and 49.7% residual activity for chymotrypsin A and B, respectively
chicken ovomucoid inhibitor
-
-
-
chloro[2,2'-(thio-kappaS)bis[ethanethiolato-kappaS]] oxorhenium(V)
-
-
chymostatin
CMTI I
-
Cucurbita maxima trypsin inhibitor, unusual interaction with the highly selective protein trypsin inhibitor from Cucurbita maxima, inhibition of Chtr1 in 0.1 M Tris-HCl, pH 7.8, with 0.5 M NaCl increased inhibitory effect
-
CMTI IV
-
Cucurbita maxima trypsin inhibitor, unusual interaction with the highly selective protein trypsin inhibitor from Cucurbita maxima, inhibition of Chtr1 in 0.1 M Tris-HCl, pH 7.8, with 0.5 M NaCl increased inhibitory effect
-
complex of vanadate and benzohydroxamic acid
-
competitive inhibitor of alpha-chymotrypsin, consisting of 1 mM vanadate and 2 mM benzohydroxamic acid
CPGT I
-
Cucurbita pepo var. giramontia inhibitor, Chtr1 in 0.1 M Tris-HCl, pH 7.8: no inhibition. With 0.5 M NaCl inhibition observed
-
CPTI IV
-
Cucurbita pepo inhibitor, Chtr1 in 0.1 M Tris-HCl, pH 7.8: no inhibition. With 0.5 M NaCl inhibition observed
-
cycloart-24-ene-3beta-ol
-
0.00014 mM, 50% inhibition, noncompetitive inhibition
cyclododecyl-6-(chloromethyl)-2-oxo-2H-1-benzopyran-3-carboxylate
-
-
cyclohexyl-6-(chloromethyl)-2-oxo-2H-1-benzopyran-3-carboxylate
-
-
cyclo[-Gly-Arg-cyclo[-S-Cys-Thr-Lys-Ser-Ile-Pro-Pro-Ile-Cys-S-]-Phe-Pro-Asp-]
-
sunflower trypsin inhibitor, SFTI-1
CyPTI VII
-
trypsin inhibitors from Cyclanthera pedata, Chtr1 in 0.1 M Tris-HCl, pH 7.8: no inhibition. With 0.5 M NaCl inhibition observed
-
dammara-20,24-dien-3beta-ol
-
0.00013 mM, 50% inhibition, competitive inhibition
diphenyl (1-[[(benzyloxy)carbonyl]amino]-2-phenylethyl)phosphonate
-
-
Eglin c
-
-
epiexcelsin
synthetic construct
-
from Commiphora mukul, noncompetitive inhibitor
faradiol 3-O-myristate
-
0.000032 mM, 50% inhibition, noncompetitive inhibition
faradiol 3-O-palmitate
-
0.000072 mM, 50% inhibition, noncompetitive inhibition
GAW
-
tripeptide
Gly-Arg-cyclo[-S-Cys-Thr-Pal-Ser-Ile-Pro-Pro-Ile-Cys-S-]-Phe-Pro-Asp
-
-
Gly-Arg-cyclo[-S-Cys-Thr-Phe(p-CH3)-Ser-Ile-Pro-Pro-Ile-Cys-S-]-Phe-Pro-Asp
-
-
Gly-Arg-cyclo[-S-Cys-Thr-Phe(p-F)-Ser-Ile-Pro-Pro-Ile-Cys-S-]-Phe-Pro-Asp
-
-
Gly-Arg-cyclo[-S-Cys-Thr-Phe(p-guanidine)-Ser-Ile-Pro-Pro-Ile-Cys-S-]-Phe-Pro-Asp
-
-
Gly-Arg-cyclo[-S-Cys-Thr-Phe(p-NH2)-Ser-Ile-Pro-Pro-Ile-Cys-S-]-Phe-Pro-Asp
-
-
Gly-Arg-cyclo[-S-Cys-Thr-Phe(p-NO2)-Ser-Ile-Pro-Pro-Ile-Cys-S-]-Phe-Pro-Asp
-
-
Gly-Arg-cyclo[-S-Cys-Thr-Tyr-Ser-Ile-Pro-Pro-Ile-Cys-S-]-Phe-Pro-Asp
-
-
IVNGEEAVPGSWPW
-
autocatalytically produced 14-residue fragment from a three-phase-partitioning-treated chymotrypsin
Leu-Phe-p-fluorophenylmethylamide
-
strong inhibition
lima bean inhibitor
lima bean trypsin inhibitior
lup-20(29)-ene-3beta,16beta-diol
-
0.00012 mM, 50% inhibition, competitive inhibition
LUTI
-
Linum usitatissimum inhibitor, inhibition of Chtr1 in 0.1 M Tris-HCl, pH 7.8, with 0.5 M NaCl increased inhibitory effect
-
maniladiol 3-O-myristate
-
0.000078 mM, 50% inhibition, competitive inhibition
maniladiol 3-O-palmitate
-
0.000084 mM, 50% inhibition, noncompetitive inhibition
methyl-4-hydroxy-4-[1-(phenylsulfonyl)pyrrol-2-yl]butanoate
-
0.0125 mg/ml, 10% inhibition
MG-132
-
reversible inhibitor
micropeptin 478-A
synthetic construct
-
potent inhibitor
micropeptin 478-B
synthetic construct
-
moderate inhibitor
micropeptin HU1021
synthetic construct
-
weak inhibitor
micropeptin HU1041
synthetic construct
-
weak inhibitor
micropeptin HU1069
synthetic construct
-
weak inhibitor
micropeptin HU895A
synthetic construct
-
moderate inhibitor
micropeptin HU895B
synthetic construct
-
potent inhibitor
micropeptin HU909
synthetic construct
-
potent inhibitor
micropeptin HU975
synthetic construct
-
moderate inhibitor
micropeptin HU989
synthetic construct
-
moderate inhibitor
mixed monolayer protected gold cluster
-
anionically functionalized amphiphilic nanoparticles
-
Mn2+
-
partially inactivates both chymotrypsin A and B at concentrations of 1 and 5 mM. At room temperature, at 5 mM concentration, 85.3% and 48.9% residual activity for chymotrypsin A and B, respectively
N-(2-acetylphenyl)-N'-(3-methylphenyl) urea
-
-
N-(2-methylphenyl)-2-oxo-1-pyrrolidine carboxamide
-
most active inhibitor
N-(tert-butoxycarbonyl)-L-alanyl-N-[4-[2-(3-oxoprop-1-yn-1-yl)phenyl]but-3-yn-1-yl]-L-phenylalaninamide
-
enediyne-amino acid conjugate
N-(tert-butoxycarbonyl)-L-alanyl-N5-[(1R)-1-(dihydroxyboranyl)-3-methylbutyl]-N1-(naphthalen-2-ylmethyl)-L-glutamamide
-
irreversible inhibitor
N-(tert-butoxycarbonyl)-L-alanyl-N6-[(1R)-1-(dihydroxyboranyl)-3-methylbutyl]-N-(naphthalen-2-ylmethyl)-6-oxo-L-lysinamide
-
irreversible inhibitor
N-(tert-butoxycarbonyl)-L-leucyl-N5-[(1R)-1-(dihydroxyboranyl)-3-methylbutyl]-N1-(naphthalen-2-ylmethyl)-L-glutamamide
-
irreversible inhibitor
N-(tert-butoxycarbonyl)-L-phenylalanyl-N5-[(1R)-1-(dihydroxyboranyl)-3-methylbutyl]-N1-(naphthalen-2-ylmethyl)-L-glutamamide
-
irreversible inhibitor
N-(tert-butoxycarbonyl)-L-valyl-N-(1-[bis[4-(sulfanylmethyl)phenoxy]phosphoryl]-2-phenylethyl)-L-prolinamide
-
tripeptide containing leucine-related phosphonates, which is a relatively weak chymotrypsin inhibitor
N-(tert-butoxycarbonyl)-L-valyl-N-[1-(diphenoxyphosphoryl)-2-phenylethyl]-L-prolinamide
-
tripeptide containing leucine-related phosphonates, which is a relatively weak chymotrypsin inhibitor
N-(tert-butoxycarbonyl)-L-valyl-N-[1-[bis(3,4,5-trimethylphenoxy)phosphoryl]-3-methylbutyl]-L-prolinamide
-
tripeptide containing leucine-related phosphonates, which is a relatively weak chymotrypsin inhibitor
N-(tert-butoxycarbonyl)-L-valyl-N-[1-[bis(4-methoxyphenoxy)phosphoryl]-3-methylbutyl]-L-prolinamide
-
tripeptide containing leucine-related phosphonates, which is a relatively weak chymotrypsin inhibitor
N-(tert-butoxycarbonyl)-L-valyl-N-[1-[bis(4-tert-butylphenoxy)phosphoryl]-3-methylbutyl]-L-prolinamide
-
tripeptide containing leucine-related phosphonates, which is a relatively weak chymotrypsin inhibitor
N-(tert-butoxycarbonyl)glycyl-N1-benzyl-N5-[(1R)-1-(dihydroxyboranyl)-3-methylbutyl]-L-glutamamide
-
irreversible inhibitor, significant inhibition of 20S proteasome chymotrypsin-like (beta5) activity, inhibitory potency is similar to that of Belactosin C
N-(tert-butoxycarbonyl)glycyl-N5-[(1R)-1-(dihydroxyboranyl)-3-methylbutyl]-N1-(naphthalen-2-ylmethyl)-L-glutamamide
-
irreversible inhibitor, possesses the greatest inhibition of the chymotrypsin-like activity, inhibitory potency is similar to that of Belactosin C. Increases the G2/M cell distribution in a similar manner to that of PS-341, and induces apoptosis at more than 0.001 mM concentration of compound in HeLa cells
N-(tert-butoxycarbonyl)glycyl-N6-[(1R)-1-(dihydroxyboranyl)-3-methylbutyl]-N-(naphthalen-2-ylmethyl)-6-oxo-L-lysinamide
-
irreversible inhibitor
N-acetyl-L-Phe-Ala-Thr-Pal aldehyde
-
-
N-acetyl-L-Phe-Ala-Thr-Phe aldehyde
-
-
N-acetyl-L-Phe-Ala-Thr-Phe-(p-NO2) aldehyde
-
-
N-acetyl-L-Phe-Ala-Thr-Tyr aldehyde
-
-
N-alpha-tosyl-L-Phe chloromethyl ketone
N-benzoyl-L-tyrosine ethyl ester
-
-
N-carbobenzoxy-Gly-Gly-Phe-chloromethyl ketone
N-p-tosyl-L-phenylalanine-chloromethyl ketone
complete inhibition at 5 mM
N-succinyl-L-phenylalanine
-
-
N-toluenesulfonyl-L-phenylalanine chloromethyl-ketone
-
at 25C, pH 8.0, in 50 mM Tris-HCl buffer and 10 mM CaCl2, partially inhibits (53% residual activity)
N-toluenesulfonyl-L-phenylalanine chloromethylketone
-
at 25C, pH 8.0, in 50 mM Tris-HCl buffer and 10 mM CaCl2, partially inhibits chymotrypsin I (56% residual activity) and chymotrypsin II (60% residual activity)
N-toluenesulfonyl-L-phenylalanine-chloromethyl ketone
-
56% residual activity at 1 mg/ml
N-tosyl-L-Phe-Ala-Thr-Phe-(p-NO2) aldehyde
-
-
N-tosyl-L-phenylalanine chloromethyl ketone
-
significant inhibition, especially to chymotrypsin B. At room temperature, at 0.05 mM concentration, 35.6% and 17.5% residual activity for chymotrypsin A and B, respectively
N-tosyl-L-phenylalanine-chloromethyl ketone
-
chymotrypsin inhibitor TPCK
N2-(tert-butoxycarbonyl)-N4-[(1R)-1-(dihydroxyboranyl)-3-methylbutyl]-N1-(naphthalen-2-ylmethyl)-L-aspartamide
-
irreversible inhibitor
N2-(tert-butoxycarbonyl)-N5-[(1R)-1-(dihydroxyboranyl)-3-methylbutyl]-N1-(naphthalen-2-ylmethyl)-L-glutamamide
-
irreversible inhibitor
N2-(tert-butoxycarbonyl)-N6-[(1R)-1-(dihydroxyboranyl)-3-methylbutyl]-N-(naphthalen-2-ylmethyl)-6-oxo-L-lysinamide
-
irreversible inhibitor
NaCl
-
continuous decrease in activity with increasing NaCl concentration, showing 95, 91, 85, 74, 68, and 63% residual activity at 5, 10, 15, 20, 25, and 30% of NaCl, respectively
p-nitrophenyl-p'-guanidino-benzoate
-
NPGB
p-octyloxybenzyltributylammonium bromide
-
5 mM, approx. 90% inactivation after 24 h
p-octyloxybenzyltrimethylammonium bromide
-
10 mM, complete inactivation
p-tosyl-L-phenylalanine chloromethane
-
-
P1 bovine chymotrypsin-bovine pancreatic trypsin inhibitor
-
P1 BPTI, P1-Xaa variants
-
pars intercerebralis major peptide
-
i.e. PMP-C, potent alpha-chymotrypsin inhibitor protein from the insect Locusta migratoria
-
Pefabloc SC
-
effectively inhibits both chymotrypsin A and B. At room temperature, at 1 mM concentration, 1.2% and 1.1% residual activity for chymotrypsin A and B, respectively
Pepstatin
-
shows minimal inhibitory effect on both chymotrypsin A and B. At room temperature, at 0.03 mM concentration, 95.9% and 92.3% residual activity for chymotrypsin A and B, respectively
phenylboronic acid
-
-
phenylmethanesulfonyl fluoride
-
effectively inhibits both chymotrypsin A and B. At room temperature, at 1 mM concentration, 0.4% and 0.3% residual activity for chymotrypsin A and B, respectively
phenylmethylsulfonyl fluoride
phenylmethylsulfonyl-fluoride
-
at 25C, pH 8.0, in 50 mM Tris-HCl buffer and 10 mM CaCl2, fully inhibits (4% residual activity)
Phenylmethylsulfonylfluoride
photolytic 1-(2-nitrophenyl)ethanol residues
-
1-(2-nitrophenyl)ethanol coated protein can be reactivated by 15 min UV-A radiation
-
Proflavin
PS-341
-
reversible inhibitor
S-(3'-chlorophenyl)-6-(chloromethyl)-2-oxo-2H-1-benzopyran-3-carbothioate
-
-
SERPINB3
partial inhibition
-
SERPINB4
partial inhibition
-
Soybean trypsin inhibitor
STI
-
soybean trypsin inhibitor, inhibition of Chtr1 in 0.1 M Tris-HCl, pH 7.8. With 0.5 M NaCl no inhibition detected
-
Suc-Val-Pro-PheP(OPh)2
-
most potent phosphonate chymotrypsin inhibitor
symplocamide A
-
from the Marine Cyanobacterium Symploca sp.. A potent cancer cell cytotoxin, which also inhibits serine proteases with a 200fold greater inhibition of chymotrypsin over trypsin
taraxast-20(39)-ene-3beta,16beta-diol
-
0.000096 mM, 50% inhibition, noncompetitive inhibition
taraxast-20-ene-3beta,16beta-diol
-
0.00016 mM, 50% inhibition, competitive inhibition
tert-butyl (2S)-2-([1-[bis(2-methylphenoxy)phosphoryl]-3-methylbutyl]carbamoyl)pyrrolidine-1-carboxylate
-
-
tert-butyl (2S)-2-([1-[bis(3,4,5-trimethylphenoxy)phosphoryl]-3-methylbutyl]carbamoyl)pyrrolidine-1-carboxylate
-
-
tert-butyl (2S)-2-([1-[bis(3,4-dimethylphenoxy)phosphoryl]-3-methylbutyl]carbamoyl)pyrrolidine-1-carboxylate
-
-
tert-butyl (2S)-2-([1-[bis(4-methoxyphenoxy)phosphoryl]-3-methylbutyl]carbamoyl)pyrrolidine-1-carboxylate
-
-
tert-butyl (2S)-2-([1-[bis(4-methylphenoxy)phosphoryl]-3-methylbutyl]carbamoyl)pyrrolidine-1-carboxylate
-
-
tert-butyl (2S)-2-[(1-[bis[4-(sulfanylmethyl)phenoxy]phosphoryl]-2-phenylethyl)carbamoyl]pyrrolidine-1-carboxylate
-
-
tert-butyl (2S)-2-[[1-(diphenoxyphosphoryl)-2-phenylethyl]carbamoyl]pyrrolidine-1-carboxylate
-
-
tert-butyl [(2S)-1-(3,4-dihydroquinolin-1(2H)-yl)-1-oxo-3-phenylpropan-2-yl]carbamate
-
isoquinoline amino acid conjugate, exhibits lesser inhibitory activity compared to the enediyne-peptide conjugates, does not interact directly with the catalytic triad of chymotrypsin
tert-butyl [(2S)-1-oxo-3-phenyl-1-(1,2,7,8-tetradehydro-5,6-dihydro-4-benzazecin-4(3H)-yl)propan-2-yl]carbamate
-
enediyne-amino acid conjugate
tirucalla-7,24-dien-3beta-ol
-
0.000098 mM, 50% inhibition, noncompetitive inhibition
tosyl-L-phenylalanine chloromethyl ketone
TPGVY
-
pentapeptide
trypsin inhibitor I-S
-
from soybean
-
trypsin-inhibitor
-
from Glycine max cv. Small Glossy Black
-
turkey ovomucoid inhibitor
-
-
-
urs-12-ene-3beta,16beta-diol
-
0.00012 mM, 50% inhibition, noncompetitive inhibition
WCI2
-
Psophocarpus tetragonolobus chymotrypsin inhibitor retards growth of Helicoverpa armigera and strongly inhibits commercially available bovine chymotrypsin
-
WCI5
-
Psophocarpus tetragonolobus chymotrypsin inhibitor retards growth of Helicoverpa armigera and strongly inhibits commercially available bovine chymotrypsin
-
wheat subtilisin/chymotrypsin inhibitor
-
as the wild-type inhibitor, the recombinant inhibitor, while acting as a strong subtilisin inhibitor, is much less efficient against alpha-chymotrypsin. Mutant E49S of the wheat subtilisin/chymotrypsin inhibitor shows a limited anti-chymotrypsin activity. Mutants E49P, Y50G and M48P/E49G are inactive against alpha-chymotrypsin
-
winged bean chymotrypsin-trypsin inhibitor
-
inhibitor from Psophocarpus tetragonolobus, inhibits and binds trypsin and chymotrypsin in a 1:1 molar ratio. Complete inhibition of chymotrypsin with purified recombinant inhibitor at a 1:1 molar ratio, similarly to the inhibitor obtained from winged bean seeds
-
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cetyltributylammonium bromide
-
promotes activity
dodecyltrimethylammonium bromide
-
2-20 mM
Hexadecyltrimethylammonium bromide
-
2-60 mM, aqueous micelles of the cationic polymeric surfactant hexadecyltrimethylammonium bromide
p-octyloxybenzyltributylammonium bromide
-
maximal activation at 5 mM, inhibition at extended incubation
tetrabutylammonium bromide
tetrapentylammonium bromide
-
-
Trypsin
-
-
-
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.5 - 1.4
4-nitrophenyl acetate
0.032 - 0.098
4-nitrophenyl benzoate
1 - 7.27
acetyl-Leu methyl ester
1.04 - 1.39
acetyl-Phe ethyl ester
0.17 - 1.12
acetyl-Trp ethyl ester
1.27 - 2
Acetyl-Tyr ethyl ester
1.05 - 6.79
Ala-Ala-Phe-7-amido-4-methylcoumarin
0.44
benzoyl-4-guanidinophenyl ester
-
25C, pH 7.6
4.94
benzoyl-D-Ala-4-guanidinophenyl ester
-
25C, pH 7.6
1.49
benzoyl-D-Leu-4-guanidinophenyl ester
-
25C, pH 7.6
2.58
benzoyl-D-Phe-4-guanidinophenyl ester
-
25C, pH 7.6
2.81
benzoyl-Gly-4-guanidinophenyl ester
-
25C, pH 7.6
2.88
benzoyl-L-Ala-4-guanidinophenyl ester
-
25C, pH 7.6
0.13 - 0.64
benzoyl-Tyr ethyl ester
7.02 - 8.72
N-acetyl-L-leucine methyl ester
0.0891
N-acetyl-L-Phe-Ala-Thr-Pal-5-amido-2-nitrobenzoic acid
-
-
0.0101
N-acetyl-L-Phe-Ala-Thr-Phe-(p-CN)-5-amido-2-nitrobenzoic acid
-
-
0.1131
N-acetyl-L-Phe-Ala-Thr-Phe-(p-COOMe)-5-amido-2-nitrobenzoic acid
-
-
0.1203
N-acetyl-L-Phe-Ala-Thr-Phe-(p-guanidine)-5-amido-2-nitrobenzoic acid
-
-
0.0635
N-acetyl-L-Phe-Ala-Thr-Phe-(p-NH2)-5-amido-2-nitrobenzoic acid
-
-
0.001
N-acetyl-L-Phe-Ala-Thr-Phe-(p-NO2)-5-amido-2-nitrobenzoic acid
-
-
0.0191
N-acetyl-L-Phe-Ala-Thr-Phe-5-amido-2-nitrobenzoic acid
-
-
0.0598
N-acetyl-L-Phe-Ala-Thr-Tyr-5-amido-2-nitrobenzoic acid
-
-
1.12 - 2.58
N-acetyl-L-phenylalanine ethyl ester
0.14 - 0.19
N-acetyl-L-tryptophan ethyl ester
0.57 - 1.17
N-Acetyl-L-tyrosine ethyl ester
0.939
N-alpha-benzoyl-DL-arginine-p-nitroanilide
-
-
0.1 - 0.27
N-benzoyl-L-Tyr ethyl ester
0.01 - 0.18
N-benzoyl-L-tyrosine 4-nitroanilide
0.12 - 6.02
N-benzoyl-L-tyrosine ethyl ester
2.23 - 20.6
N-benzoyl-L-tyrosine methyl ester
0.05 - 0.083
N-benzoyl-L-tyrosine-4-nitroanilide
0.08 - 0.21
N-benzoyl-Tyr p-nitroanilide
0.0009
N-benzyloxycarbonyl-L-Phe-Ala-Thr-Phe-(p-NO2)-5-amido-2-nitrobenzoic acid
-
-
0.0177
N-benzyloxycarbonyl-L-Phe-Ala-Thr-Tyr-5-amido-2-nitrobenzoic acid
-
-
0.32 - 6.1
N-glutaryl-L-Phe-p-nitroanilide
0.0013 - 0.023
N-succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin
0.32 - 3
N-succinyl-Ala-Ala-Pro-Arg-p-nitroanilide
0.0346
N-succinyl-Ala-Ala-Pro-Leu-4-nitroanilide
-
pH 8.5, temperature not specified in the publication
-
0.26 - 0.44
N-succinyl-Ala-Ala-Pro-Leu-p-nitroanilide
0.32 - 2.6
N-succinyl-Ala-Ala-Pro-Lys-p-nitroanilide
0.02 - 0.37
N-succinyl-Ala-Ala-Pro-Met-p-nitroanilide
0.052 - 0.58
N-succinyl-Ala-Ala-Pro-Nle-p-nitroanilide
0.16 - 1.6
N-succinyl-Ala-Ala-Pro-Nva-p-nitroanilide
0.05 - 0.19
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
0.012
N-succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin
-
pH 8.5, temperature not specified in the publication
0.0074 - 1.1
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide
0.4 - 0.48
N-succinyl-Ala-Phe-Ala
2.5
N-succinyl-Ala-Phe-p-nitroanilide
-
30C, pH 8.0
0.044
N-succinyl-L-Ala-Ala-Phe-p-nitroanilide
-
at 25C
0.074
N-succinyl-L-Ala-Ala-Pro-p-nitroanilide
-
in 50 mM Tris-HCl pH 8.0 with 10 mM CaCl2, at 30 C
0.14
N-succinyl-L-Ala-Ala-Pro-Phe-p-nitroanilide
-
at 25C
1.09
N-succinyl-L-Phe-p-nitroanilide
-
at 25C
0.024 - 0.375
N-succinyl-L-phenylalanine-4-nitroanilide
0.012
N-succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin
-
pH 8.5, temperature not specified in the publication
0.01 - 0.032
N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin
2.3 - 5
N-succinyl-Phe-7-amido-4-methylcoumarin
0.09 - 0.1
Nalpha-benzyloxycarbonyl-L-Ala p-nitrophenyl ester
0.62 - 0.64
Nalpha-benzyloxycarbonyl-L-Lys p-nitrophenyl ester
0.08
Nalpha-benzyloxycarbonyl-L-Tyr p-nitrophenyl ester
-
-
0.4
Nalpha-tosyl-L-Tyr benzyl ester
-
-
0.0297 - 0.044
p-nitrophenyl acetate
0.072
Suc-Ala-Ala-Pro-Phe-4-nitroanilide
-
pH 9.0, 25C
0.022 - 0.07
succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin
0.048 - 0.074
succinyl-Ala-Ala-Pro-Phe-p-nitroanilide
0.012
succinyl-Ala-Ala-Pro-Tyr-7-amido-4-methylcoumarin
-
37C, pH 8.0
0.09
succinyl-L-Ala-Ala-Pro-L-phenylalanine-p-nitroanilide
-
at 30C
0.054
succinyl-L-Ala-L-Ala-L-Pro-L-Leu-4-nitroanilide
-
pH 8.5, 25C
0.022 - 0.12
succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide
0.0005 - 0.8
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin
additional information
additional information
-
Michaelis-Menten metabolic rate constants (Vmax and Km) are calculated by nonlinear regression for chymotrypsin, the Vmax is 97.97 nmol/l/min and Km is 7.84 microM
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.015 - 0.1
4-nitrophenyl acetate
0.0075 - 0.047
4-nitrophenyl benzoate
6.6 - 22.1
acetyl-Leu methyl ester
5.3 - 52.4
acetyl-Phe ethyl ester
9.3 - 48.4
acetyl-Trp ethyl ester
25.4 - 126
Acetyl-Tyr ethyl ester
0.66 - 21.7
Ala-Ala-Phe-7-amido-4-methylcoumarin
0.13
benzoyl-4-guanidinophenyl ester
Bos taurus
-
25C, pH 7.6
0.56 - 6.08
benzoyl-D-Ala-4-guanidinophenyl ester
3 - 6
benzoyl-D-Leu-4-guanidinophenyl ester
5.54
benzoyl-D-Phe-4-guanidinophenyl ester
Bos taurus
-
25C, pH 7.6
1.18 - 6.08
benzoyl-Gly-4-guanidinophenyl ester
10.4
benzoyl-L-Ala-4-guanidinophenyl ester
Bos taurus
-
25C, pH 7.6
57.5 - 76.6
benzoyl-Tyr ethyl ester
4.6 - 6.5
N-acetyl-L-leucine methyl ester
6.4
N-acetyl-L-Phe-Ala-Thr-Pal-5-amido-2-nitrobenzoic acid
Bos taurus
-
-
3
N-acetyl-L-Phe-Ala-Thr-Phe-(p-CN)-5-amido-2-nitrobenzoic acid
Bos taurus
-
-
0.3
N-acetyl-L-Phe-Ala-Thr-Phe-(p-COOMe)-5-amido-2-nitrobenzoic acid
Bos taurus
-
-
6.5
N-acetyl-L-Phe-Ala-Thr-Phe-(p-guanidine)-5-amido-2-nitrobenzoic acid
Bos taurus
-
-
16.2
N-acetyl-L-Phe-Ala-Thr-Phe-(p-NH2)-5-amido-2-nitrobenzoic acid
Bos taurus
-
-
0.6
N-acetyl-L-Phe-Ala-Thr-Phe-(p-NO2)-5-amido-2-nitrobenzoic acid
Bos taurus
-
-
6.1
N-acetyl-L-Phe-Ala-Thr-Phe-5-amido-2-nitrobenzoic acid
Bos taurus
-
-
22.7
N-acetyl-L-Phe-Ala-Thr-Tyr-5-amido-2-nitrobenzoic acid
Bos taurus
-
-
40.3 - 54.8
N-acetyl-L-phenylalanine ethyl ester
25.7 - 48.8
N-acetyl-L-tryptophan ethyl ester
130 - 220
N-acetyl-L-Tyr-ethyl ester
0.1 - 0.19
N-acetyl-L-Tyr-p-nitroanilide
82.8 - 120
N-Acetyl-L-tyrosine ethyl ester
0.611
N-alpha-benzoyl-DL-arginine-p-nitroanilide
Bos taurus
-
-
43 - 329
N-benzoyl-L-Tyr ethyl ester
0.013 - 2.3
N-benzoyl-L-tyrosine 4-nitroanilide
0.03 - 70.4
N-benzoyl-L-tyrosine ethyl ester
2.34 - 10.8
N-benzoyl-L-tyrosine methyl ester
0.04 - 0.5
N-benzoyl-L-tyrosine-4-nitroanilide
0.05 - 0.82
N-benzoyl-Tyr p-nitroanilide
1.8
N-benzyloxycarbonyl-L-Phe-Ala-Thr-Phe-(p-NO2)-5-amido-2-nitrobenzoic acid
Bos taurus
-
-
16.7
N-benzyloxycarbonyl-L-Phe-Ala-Thr-Tyr-5-amido-2-nitrobenzoic acid
Bos taurus
-
-
0.015 - 0.31
N-glutaryl-L-Phe-p-nitroanilide
0.014 - 0.05
N-succinyl-Ala-Ala-Pro-Arg-p-nitroanilide
0.55
N-succinyl-Ala-Ala-Pro-Leu-4-nitroanilide
Musca domestica
-
pH 8.5, temperature not specified in the publication
-
0.031 - 16
N-succinyl-Ala-Ala-Pro-Leu-p-nitroanilide
0.006 - 0.34
N-succinyl-Ala-Ala-Pro-Lys-p-nitroanilide
1.45 - 15
N-succinyl-Ala-Ala-Pro-Met-p-nitroanilide
12 - 18
N-succinyl-Ala-Ala-Pro-Nle-p-nitroanilide
0.04 - 9.31
N-succinyl-Ala-Ala-Pro-Nva-p-nitroanilide
3.8 - 13.5
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
42
N-succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin
Musca domestica
-
pH 8.5, temperature not specified in the publication
10.58 - 110
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide
0.9 - 1.2
N-succinyl-Ala-Phe-Ala
0.65
N-succinyl-Ala-Phe-p-nitroanilide
Bos taurus
-
30C, pH 8.0
39
N-succinyl-L-Ala-Ala-Phe-p-nitroanilide
Bos taurus
-
at 25C
18.6
N-succinyl-L-Ala-Ala-Pro-p-nitroanilide
Sardinops sagax
-
in 50 mM Tris-HCl pH 8.0 with 10 mM CaCl2, at 30 C
7.8
N-succinyl-L-Ala-Ala-Pro-Phe-p-nitroanilide
Bos taurus
-
at 25C
0.014
N-succinyl-L-Phe-p-nitroanilide
Bos taurus
-
at 25C
0.154 - 1.177
N-succinyl-L-phenylalanine-4-nitroanilide
23.9
N-succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin
Musca domestica
-
pH 8.5, temperature not specified in the publication
0.008 - 6.43
Nalpha-benzyloxycarbonyl-L-Ala p-nitrophenyl ester
0.6
Nalpha-benzyloxycarbonyl-L-Lys p-nitrophenyl ester
Bos taurus
-
at pH 4.0
85
Nalpha-benzyloxycarbonyl-L-Tyr p-nitrophenyl ester
Bos taurus
-
-
21.4
Nalpha-tosyl-L-Tyr benzyl ester
Bos taurus
-
-
0.017 - 0.028
p-nitrophenyl acetate
19.24
Suc-Ala-Ala-Pro-Phe-4-nitroanilide
Pterygoplichthys disjunctivus
-
pH 9.0, 25C
98.3
succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin
Rattus norvegicus
-
37C, pH 8.0
4.8 - 18.6
succinyl-Ala-Ala-Pro-Phe-p-nitroanilide
105
succinyl-Ala-Ala-Pro-Tyr-7-amido-4-methylcoumarin
Rattus norvegicus
-
37C, pH 8.0
1.9
succinyl-L-Ala-Ala-Pro-L-phenylalanine-p-nitroanilide
Bos taurus
-
at 30C
3.6
succinyl-L-Ala-L-Ala-L-Pro-L-Leu-4-nitroanilide
Sepia officinalis
-
pH 8.5, 25C
18 - 21.5
succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide
0.9 - 3.4
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin
additional information
benzoyl-D-Phe-4-guanidinophenyl ester
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.021 - 0.144
4-nitrophenyl acetate
0.141 - 0.668
4-nitrophenyl benzoate
0.23 - 220
N-benzoyl-L-tyrosine 4-nitroanilide
0.48 - 10
N-benzoyl-L-tyrosine-4-nitroanilide
16
N-succinyl-Ala-Ala-Pro-Leu-4-nitroanilide
Musca domestica
-
pH 8.5, temperature not specified in the publication
195744
34
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
Musca domestica
-
pH 8.5, temperature not specified in the publication
953
3500
N-succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin
Musca domestica
-
pH 8.5, temperature not specified in the publication
161645
2.74 - 6.47
N-succinyl-L-phenylalanine-4-nitroanilide
2000
N-succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin
Musca domestica
-
pH 8.5, temperature not specified in the publication
33514
2670
Suc-Ala-Ala-Pro-Phe-4-nitroanilide
Pterygoplichthys disjunctivus
-
pH 9.0, 25C
6944
21
succinyl-L-Ala-Ala-Pro-L-phenylalanine-p-nitroanilide
Bos taurus
-
at 30C
40535
70
succinyl-L-Ala-L-Ala-L-Pro-L-Leu-4-nitroanilide
Sepia officinalis
-
pH 8.5, 25C
168018
179 - 820
succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide
1100 - 6800
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.08
(Cbz-alanyl)aminomethyl boronic acid
-
37C, pH 8.0
1.96
(Cbz-phenylalanyl)aminomethyl boronic acid
-
37C, pH 8.0
0.00001
2,2'-[[(1-[[(benzyloxy)carbonyl]amino]-2-phenylethyl)phosphoryl]bis(oxybenzene-4,1-diyl)]diacetic acid
-
in 100 mM HEPES buffer, 500 mM NaCl, pH 7.5, 9% dimethyl sulfoxide
0.0027
2,2'-[[(1-[[1-(tert-butoxycarbonyl)-L-prolyl]amino]-3-methylbutyl)phosphoryl]bis(oxybenzene-3,1-diyl)]diacetic acid