Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.21.1 extracted from

  • Verma, S.; Ghosh, K.
    Effect of cationic surfactants on the enzymatic activity of alpha-chymotrypsin (2011), Kinet. Catal., 52, 6-10.
No PubMed abstract available
Search for more literature for 3.4.21.1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.032
-
 4-nitrophenyl benzoate presence of 2.0 mM cetyltributylphosphonium bromide, pH 7.8, 27°C Bos taurus
0.035
-
 4-nitrophenyl benzoate presence of 2.0 mM cetyltrimethylammonium bromide, pH 7.8, 27°C Bos taurus
0.048
-
 4-nitrophenyl benzoate presence of 0.5 mM cetyltributylphosphonium bromide, pH 7.8, 27°C Bos taurus
0.053
-
 4-nitrophenyl benzoate pH 7.8, 27°C Bos taurus
0.06
-
 4-nitrophenyl benzoate presence of 0.5 mM cetyltrimethylammonium bromide, pH 7.8, 27°C Bos taurus
0.08
-
 4-nitrophenyl benzoate presence of 2.0 mM cetyltriphenylphosphonium bromide, pH 7.8, 27°C Bos taurus
0.098
-
 4-nitrophenyl benzoate presence of 0.5 mM cetyltriphenylphosphonium bromide, pH 7.8, 27°C Bos taurus

Organic Solvent Stability

Organic Solvent Comment Organism
additional information effect of cationic surfactants cetyltriphenylphosphonium bromide, cetyltributylphosphonium bromide and cetyltrimethylammonium bromide on the hydrolysis of 4-nitrophenyl benzoate catalyzed by alpha-chymotrypsin. The ester is hydrolyzed readily in all the surfactants with the highest activity shown in cetyltributylphosphonium bromide Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus P00766
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
 Bos taurus
-
pancreas
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl benzoate + H2O
-
 Bos taurus 4-nitrophenol + benzoate
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0075
-
 4-nitrophenyl benzoate pH 7.8, 27°C Bos taurus
0.0086
-
 4-nitrophenyl benzoate presence of 0.5 mM cetyltrimethylammonium bromide, pH 7.8, 27°C Bos taurus
0.013
-
 4-nitrophenyl benzoate presence of 2.0 mM cetyltrimethylammonium bromide, pH 7.8, 27°C Bos taurus
0.017
-
 4-nitrophenyl benzoate presence of 0.5 mM cetyltributylphosphonium bromide, pH 7.8, 27°C Bos taurus
0.0214
-
 4-nitrophenyl benzoate presence of 2.0 mM cetyltributylphosphonium bromide, pH 7.8, 27°C Bos taurus
0.024
-
 4-nitrophenyl benzoate presence of 0.5 mM cetyltriphenylphosphonium bromide, pH 7.8, 27°C Bos taurus
0.047
-
 4-nitrophenyl benzoate presence of 2.0 mM cetyltriphenylphosphonium bromide, pH 7.8, 27°C Bos taurus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.141
-
 4-nitrophenyl benzoate pH 7.8, 27°C Bos taurus
0.143
-
 4-nitrophenyl benzoate presence of 0.5 mM cetyltrimethylammonium bromide, pH 7.8, 27°C Bos taurus
0.247
-
 4-nitrophenyl benzoate presence of 0.5 mM cetyltriphenylphosphonium bromide, pH 7.8, 27°C Bos taurus
0.345
-
 4-nitrophenyl benzoate presence of 0.5 mM cetyltributylphosphonium bromide, pH 7.8, 27°C Bos taurus
0.365
-
 4-nitrophenyl benzoate presence of 2.0 mM cetyltrimethylammonium bromide, pH 7.8, 27°C Bos taurus
0.583
-
 4-nitrophenyl benzoate presence of 2.0 mM cetyltriphenylphosphonium bromide, pH 7.8, 27°C Bos taurus
0.668
-
 4-nitrophenyl benzoate presence of 2.0 mM cetyltributylphosphonium bromide, pH 7.8, 27°C Bos taurus