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Literature summary for 3.4.21.1 extracted from

  • Valiullina, Y.; Ermakova, E.; Faizullin, D.; Mirgorodskaya, A.; Zuev, Y.
    Structure and properties of complexes of α-chymotrypsin with hydroxyl-containing gemini dicationic surfactants with a spacer moiety of varying length (2014), J. Struct. Chem., 55, 1556-1564.
No PubMed abstract available
Search for more literature for 3.4.21.1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
 N-benzoyl-L-tyrosine-4-nitroanilide presence of alpha,omega-alkanedyl-bis(hydroxyethylmethylcetyl) dibromide with a polymethylene spacer n-(CH2)10, pH 7.6, 25°C Bos taurus
0.07
-
 N-benzoyl-L-tyrosine-4-nitroanilide pH 7.6, 25°C Bos taurus
0.083
-
 N-benzoyl-L-tyrosine-4-nitroanilide presence of alpha,omega-alkanedyl-bis(hydroxyethylmethylcetyl) dibromide with a polymethylene spacer n-(CH2)6, pH 7.6, 25°C Bos taurus

Organic Solvent Stability

Organic Solvent Comment Organism
additional information supramolecular complexes of alpha-chymotrypsin with hydroxyl-containing alkyl ammonium gemini surfactants, i.e. alpha,omega-alkanedyl-bis(hydroxyethylmethylcetyl ammonium dibromides), with a polymethylene spacer of varying length. The interaction of the surfactants with alpha-chymotrypsin leads to changes of different intensity in the structural state of proteins. A correlation is found between the activity of alpha-chymotrypsin and the length of the spacer moiety. The enzyme activity correlates with the change in the substrate concentration in the aqueous phase of the surfactant micellar solution Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus P00766
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
 Bos taurus
-
pancreas
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-benzoyl-L-tyrosine-4-nitroanilide + H2O
-
 Bos taurus N-benzoyl-L-tyrosine + 4-nitroaniline
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.04
-
 N-benzoyl-L-tyrosine-4-nitroanilide presence of alpha,omega-alkanedyl-bis(hydroxyethylmethylcetyl) dibromide with a polymethylene spacer n-(CH2)6, pH 7.6, 25°C Bos taurus
0.15
-
 N-benzoyl-L-tyrosine-4-nitroanilide pH 7.6, 25°C Bos taurus
0.5
-
 N-benzoyl-L-tyrosine-4-nitroanilide presence of alpha,omega-alkanedyl-bis(hydroxyethylmethylcetyl) dibromide with a polymethylene spacer n-(CH2)10, pH 7.6, 25°C Bos taurus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.48
-
 N-benzoyl-L-tyrosine-4-nitroanilide presence of alpha,omega-alkanedyl-bis(hydroxyethylmethylcetyl) dibromide with a polymethylene spacer n-(CH2)6, pH 7.6, 25°C Bos taurus
2.14
-
 N-benzoyl-L-tyrosine-4-nitroanilide pH 7.6, 25°C Bos taurus
10
-
 N-benzoyl-L-tyrosine-4-nitroanilide presence of alpha,omega-alkanedyl-bis(hydroxyethylmethylcetyl) dibromide with a polymethylene spacer n-(CH2)10, pH 7.6, 25°C Bos taurus