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Literature summary for 3.4.21.1 extracted from

  • Balti, R.; Bougherra, F.; Bougatef, A.; Hayet, B.; Nedjar-Arroume, N.; Dhulster, P.; Guillochon, D.; Nasri, M.
    Chymotrypsin from the hepatopancreas of cuttlefish (Sepia officinalis) with high activity in the hydrolysis of long chain peptide substrates: Purification and biochemical characterisation (2012), Food Chem., 130, 475-484.
    View publication on PubMed
Search for more literature for 3.4.21.1

Inhibitors

Inhibitors Comment Organism Structure
chymostatin 0.01 mM, no residual activity Sepia officinalis
Cu2+ 5 mM, 1.4% residual activity Sepia officinalis
DFP 0.1 mM, 6.3% residual activity Sepia officinalis
Fe2+ 5 mM, 6% residual activity Sepia officinalis
Hg2+ 5 mM, 12% residual activity Sepia officinalis
phenylmethylsulfonyl fluoride 0.1 mM, 1.4% residual activity Sepia officinalis
Soybean trypsin inhibitor 0.0015 mM, 2.1% residual activity Sepia officinalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.022
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide pH 8.5, 25°C Sepia officinalis
0.054
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Leu-4-nitroanilide pH 8.5, 25°C Sepia officinalis

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 5 mM, 110% of initial activity Sepia officinalis
Co2+ 5 mM, 120% of initial activity Sepia officinalis
Mg2+ 5 mM, 117% of initial activity Sepia officinalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
 x * 28000, SDS-PAGE Sepia officinalis

Organism

Organism UniProt Comment Textmining
Sepia officinalis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sepia officinalis

Source Tissue

Source Tissue Comment Organism Textmining
hepatopancreas
-
 Sepia officinalis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4
-
 substrate benzyloxycarbonyl-L-Ala-L-Ala-L-Leu-4-nitroanilide, pH 8.5, 25°C Sepia officinalis
15
-
 substrate succinyl-L-Ala-L-Ala-L-Pro-L-Leu-4-nitroanilide, pH 8.5, 25°C Sepia officinalis
69
-
 substrate succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide, pH 8.5, 25°C Sepia officinalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-L-Ala-L-Ala-L-Leu-4-nitroanilide + H2O
-
 Sepia officinalis benzyloxycarbonyl-L-Ala-L-Ala-L-Leu + 4-nitroaniline
-
 ?
succinyl-L-Ala-L-Ala-L-Pro-L-Leu-4-nitroanilide + H2O
-
 Sepia officinalis succinyl-L-Ala-L-Ala-L-Pro-L-Leu + 4-nitroaniline
-
 ?
succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide + H2O
-
 Sepia officinalis succinyl-L-Ala-L-Ala-L-Pro-L-Phe + 4-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
? x * 28000, SDS-PAGE Sepia officinalis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Sepia officinalis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40
-
 66% of maximum activity Sepia officinalis
50
-
 85% of maximum activity Sepia officinalis
60
-
 86% of maximum activity Sepia officinalis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Leu-4-nitroanilide pH 8.5, 25°C Sepia officinalis
18
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide pH 8.5, 25°C Sepia officinalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Sepia officinalis

pH Range

pH Minimum pH Maximum Comment Organism
7.5 9.5 more than 92% of maximum activity within Sepia officinalis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
70
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Leu-4-nitroanilide pH 8.5, 25°C Sepia officinalis
820
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide pH 8.5, 25°C Sepia officinalis