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Literature summary for 3.4.21.1 extracted from

  • Moyano, F.; Setien, E.; Silber, J.J.; Correa, N.M.
    Enzymatic hydrolysis of N-benzoyl-L-tyrosine p-nitroanilide by ?-chymotrypsin in DMSO-water/AOT/n-heptane reverse micelles. A unique interfacial effect on the enzymatic activity (2013), Langmuir, 29, 8245-8254.
    View publication on PubMed
Search for more literature for 3.4.21.1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 N-benzoyl-L-tyrosine 4-nitroanilide presence of 7% dimethyl sulfoxide, pH 8.7, 25°C Bos taurus
0.056
-
 N-benzoyl-L-tyrosine 4-nitroanilide presence of 15% dimethyl sulfoxide, pH 8.7, 25°C Bos taurus
0.16
-
 N-benzoyl-L-tyrosine 4-nitroanilide water-dimethyl sulfoxide mixture encapsulated in 1,4-bis-2-ethylhexylsulfosuccinate/n-heptane reverse micelles, 7% dimethyl sulfoxide-water, pH 8.7, 25°C Bos taurus
0.18
-
 N-benzoyl-L-tyrosine 4-nitroanilide water-dimethyl sulfoxide mixture encapsulated in 1,4-bis-2-ethylhexylsulfosuccinate/n-heptane reverse micelles, 20% dimethyl sulfoxide-water, pH 8.7, 25°C Bos taurus

Organic Solvent Stability

Organic Solvent Comment Organism
dimethyl sulfoxide study on the influence of different water-dimethyl sulfoxide mixtures encapsulated in 1,4-bis-2-ethylhexylsulfosuccinate/n-heptane reverse micelles on the enzymatic hydrolysis of N-benzoyl-L-tyrosine p-nitroanilide by alpha-chymotrypsin. The enzyme dissolved in a 20% molar ratio of the dimethyl sulfoxide-water mixture does not present enzymatic activity. There is preferential solvation of the 1,4-bis-2-ethylhexylsulfosuccinate reverse micelle interface by water molecules. The kinetic parameters are determined at fixed ratio ([water] + [dimethyl sulfoxide])/[1,4-bis-2-ethylhexylsulfosuccinate] = 20 at different dimethyl sulfoxide-water compositions. Michaelis-Menten mechanism is valid for alpha-chymotrypsin in all the reverse micelle systems studied and the reaction takes place at the reverse micelle interface. The enzyme encapsulated by the reverse micelles shows catalytic effects with similar kcat/KM values at any dimethyl sulfoxide composition investigated Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus P00766
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
 Bos taurus
-
pancreas
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-benzoyl-L-tyrosine 4-nitroanilide + H2O
-
 Bos taurus N-benzoyl-L-tyrosine + 4-nitroaniline
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.013
-
 N-benzoyl-L-tyrosine 4-nitroanilide presence of 15% dimethyl sulfoxide, pH 8.7, 25°C Bos taurus
0.04
-
 N-benzoyl-L-tyrosine 4-nitroanilide water-dimethyl sulfoxide mixture encapsulated in 1,4-bis-2-ethylhexylsulfosuccinate/n-heptane reverse micelles, 7% dimethyl sulfoxide-water, pH 8.7, 25°C Bos taurus
0.055
-
 N-benzoyl-L-tyrosine 4-nitroanilide water-dimethyl sulfoxide mixture encapsulated in 1,4-bis-2-ethylhexylsulfosuccinate/n-heptane reverse micelles, 20% dimethyl sulfoxide-water, pH 8.7, 25°C Bos taurus
2.3
-
 N-benzoyl-L-tyrosine 4-nitroanilide presence of 7% dimethyl sulfoxide, pH 8.7, 25°C Bos taurus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.23
-
 N-benzoyl-L-tyrosine 4-nitroanilide presence of 15% dimethyl sulfoxide, pH 8.7, 25°C Bos taurus
0.254
-
 N-benzoyl-L-tyrosine 4-nitroanilide water-dimethyl sulfoxide mixture encapsulated in 1,4-bis-2-ethylhexylsulfosuccinate/n-heptane reverse micelles, 7% dimethyl sulfoxide-water, pH 8.7, 25°C Bos taurus
0.305
-
 N-benzoyl-L-tyrosine 4-nitroanilide water-dimethyl sulfoxide mixture encapsulated in 1,4-bis-2-ethylhexylsulfosuccinate/n-heptane reverse micelles, 20% dimethyl sulfoxide-water, pH 8.7, 25°C Bos taurus
220
-
 N-benzoyl-L-tyrosine 4-nitroanilide presence of 7% dimethyl sulfoxide, pH 8.7, 25°C Bos taurus