Literature summary for 3.4.21.1 extracted from

Paulus, A.; Morhardt, C.; Lehle, N.; Franzreb, M.
Recovery of chymotrypsin using magnetic particles and aqueous micellar two-phase systems: Influence of non-ionic surfactants on enzyme activity (2014), J. Mol. Catal. B, 110, 165-170.

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Application

Application	Comment	Organism
synthesis	use of immobilized bovine alpha-chymotrypsin in a biotransformation process applying an aqueous micellar two-phase system for separation and recycling of the enzyme immobilisates. The thermoresponsive surfactant, Eumulgin ES, has no influence on the activity up to a concentration of 10%, while surfactants Tween 20, Triton X 114, Triton X 100 lead to an activity reduction. For Triton X-114, the activity rises to its previous value after washing or a buffer exchange influence. Optimization of the aqueous micellar two-phase system composition via a Design of Experiments approach allows for reuse of the immobilisates for hemoglobin digestion over eleven cycles in an Eumulgin ES aqueous micellar two-phase system	Bos taurus

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	P00766	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
commercial preparation	-	Bos taurus	-
pancreas	-	Bos taurus	-

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Release 2023.1 (January 2023)