Information on EC 4.1.3.1 - isocitrate lyase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea

EC NUMBER
COMMENTARY
4.1.3.1
-
RECOMMENDED NAME
GeneOntology No.
isocitrate lyase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
isocitrate = succinate + glyoxylate
show the reaction diagram
-
-
-
-
isocitrate = succinate + glyoxylate
show the reaction diagram
kinetic mechanism is the same in microgravity and in standard g controls
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
condensation
-
-
-
-
elimination
-
-
of an oxo-acid, C-C bond cleavage
-
PATHWAY
KEGG Link
MetaCyc Link
Glyoxylate and dicarboxylate metabolism
-
glyoxylate cycle
-
Metabolic pathways
-
Microbial metabolism in diverse environments
-
TCA cycle IV (2-oxoglutarate decarboxylase)
-
TCA cycle V (2-oxoglutarate:ferredoxin oxidoreductase)
-
SYSTEMATIC NAME
IUBMB Comments
isocitrate glyoxylate-lyase (succinate-forming)
The isomer of isocitrate involved is (1R,2S)-1-hydroxypropane-1,2,3-tricarboxylate [3].
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
acuD
Penicillium marneffei
-
citrate lyase encoding gene
acuD
Penicillium marneffei CBS 119456
-
citrate lyase encoding gene
-
citrate lyase
-
-
citrate lyase
-
-
citrate lyase
Debaryomyces hansenii Y7426
-
-
-
citrate lyase
Penicillium marneffei
-
-
citrate lyase
Penicillium marneffei CBS 119456
-
-
-
EgGCE
Q8LPA6
bifunctional enzyme: isocitrate lyase and malate synthase
EgGCE
Euglena gracilis SM-ZK
Q8LPA6
bifunctional enzyme: isocitrate lyase and malate synthase
-
FPICL1
Q58A52
-
FPICL1
Fomitopsis palustris TYP-6137
Q58A52
-
-
ICL
-
-
-
-
ICL
Aspergillus fumigatus ATCC46645
-
-
-
ICL
Aspergillus niger N402
-
-
-
ICL
Colwellia psychrerythraea NRC 1004
A8C5K8
-
-
ICL
Debaryomyces hansenii Y7426
-
-
-
ICL
Escherichia coli K-12 W3110
-
-
-
ICL
Q8LPA6
-
ICL
Euglena gracilis SM-ZK
Q8LPA6
-
-
ICL
Fomitopsis palustris TYP-6137
Q58A52
-
-
ICL
-
key enzyme in the glyoxylate cycle
ICL
Magnaporthe grisea Guy 11
-
key enzyme in the glyoxylate cycle
-
ICL
Mycobacterium smegmatis 1-2c
-
-
-
ICL
-
ICL1 with isocitrate and 2-methylisocitrate lyase activity
ICL
Pseudomonas fluorescens 13525
-
-
-
ICL
Sinorhizobium meliloti Rm5000
-
-
-
ICL
-
-
ICL1
-
isoenzyme, has both isocitrate lyase and 2-methylisocitrate lyase activity
ICL2
-
isoenzyme, has no 2-methylisocitrate lyase activity
isocitrase
-
-
-
-
isocitratase
-
-
-
-
isocitrate lyase
-
-
isocitrate lyase
-
-
isocitrate lyase
Aspergillus niger N402
-
-
-
isocitrate lyase
-
-
isocitrate lyase
-
-
isocitrate lyase
Candida albicans ATCC 10231
-
;
-
isocitrate lyase
-
-
isocitrate lyase
Q8LPA6
activity of a bifunctional protein named glyoxylate cycle enzyme (malate synthase and isocitrate lyase activity)
isocitrate lyase
-
-
isocitrate lyase
Mycobacterium smegmatis 1-2c
-
-
-
isocitrate lyase
P0A5H3
-
isocitrate lyase
-
-
isocitrate lyase
Q5YLB6
-
isocitrate lyase
Penicillium marneffei
-
-
isocitrate lyase
-
-
isocitrate lyase
-
-
isocitrate lyase
-
-
isocitrate lyase 1
-
relative electrophoretic mobility Rf 0.28
isocitrate lyase 2
-
relative electrophoretic mobility Rf 0.44
isocitrate lyase 2
-
-
isocitric lyase
-
-
-
-
isocitritase
-
-
-
-
lyase, isocitrate
-
-
-
-
petal death protein
Q05957
-
SSO1333
Q97YI8
gene name
threo-DS-Isocitrate glyoxylate-lyase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
9045-78-7
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
strain ATCC46645
-
-
Manually annotated by BRENDA team
Aspergillus fumigatus ATCC46645
strain ATCC46645
-
-
Manually annotated by BRENDA team
strain N402
-
-
Manually annotated by BRENDA team
Aspergillus niger N402
strain N402
-
-
Manually annotated by BRENDA team
thermophilic
-
-
Manually annotated by BRENDA team
strains 1026b and DD503
-
-
Manually annotated by BRENDA team
ATCC 10231
-
-
Manually annotated by BRENDA team
Candida albicans ATCC 10231
-
-
-
Manually annotated by BRENDA team
Candida brassicae
-
-
-
Manually annotated by BRENDA team
strain NRC 1004
UniProt
Manually annotated by BRENDA team
Colwellia psychrerythraea NRC 1004
strain NRC 1004
UniProt
Manually annotated by BRENDA team
Debaryomyces hansenii Y7426
Y7426
-
-
Manually annotated by BRENDA team
strain K-12 W3110
-
-
Manually annotated by BRENDA team
strains BW2952, BW3767, BW3709, BW3480, BW3708, BW3782, MG1655
-
-
Manually annotated by BRENDA team
Escherichia coli K-12 W3110
strain K-12 W3110
-
-
Manually annotated by BRENDA team
-
Q8LPA6
Swissprot
Manually annotated by BRENDA team
SM-ZK
Q8LPA6
Swissprot
Manually annotated by BRENDA team
Euglena gracilis SM-ZK
SM-ZK
Q8LPA6
Swissprot
Manually annotated by BRENDA team
strain TYP-6137, formerly named Tyromyces palustris
EMBL
Manually annotated by BRENDA team
Fomitopsis palustris TYP-6137
strain TYP-6137, formerly named Tyromyces palustris
EMBL
Manually annotated by BRENDA team
soybean
-
-
Manually annotated by BRENDA team
sunflower
-
-
Manually annotated by BRENDA team
Klicl1 deletion mutant
-
-
Manually annotated by BRENDA team
Lupinus sp.
-
-
-
Manually annotated by BRENDA team
strain Guy 11
-
-
Manually annotated by BRENDA team
Magnaporthe grisea Guy 11
strain Guy 11
-
-
Manually annotated by BRENDA team
Mycobacterium smegmatis 1-2c
1-2c
-
-
Manually annotated by BRENDA team
H37Rv, ATCC 25618, without functional ICL2 gene (aceA) because gene is split into two ORFs (aceAa and aceAb)
-
-
Manually annotated by BRENDA team
ssp. indica cv Guangluai
-
-
Manually annotated by BRENDA team
Penicillium marneffei
-
-
-
Manually annotated by BRENDA team
Penicillium marneffei
CBS 119456
-
-
Manually annotated by BRENDA team
Penicillium marneffei CBS 119456
CBS 119456
-
-
Manually annotated by BRENDA team
strain PAO1
UniProt
Manually annotated by BRENDA team
strain PAO1
-
-
Manually annotated by BRENDA team
strain 13525
-
-
Manually annotated by BRENDA team
Pseudomonas fluorescens 13525
strain 13525
-
-
Manually annotated by BRENDA team
wild-type: KT2442, mutant: KT217, poly(3-hydroxyalkanoate)-overproducing mutant in which aceA gene is knocked out
-
-
Manually annotated by BRENDA team
serovar typhimurium
-
-
Manually annotated by BRENDA team
strain Rm5000
-
-
Manually annotated by BRENDA team
Sinorhizobium meliloti Rm5000
strain Rm5000
-
-
Manually annotated by BRENDA team
Streptomyces clavuligerus NRRL 3585
NRRL 3585
SwissProt
Manually annotated by BRENDA team
strain DSM 639
-
-
Manually annotated by BRENDA team
isolate ATCC 95640
-
-
Manually annotated by BRENDA team
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5
8.5
-
50% activity at pH 5, 22% activity at pH 8.5, no activity at pH 9
6.9
7.9
-
50% of optimal activity at pH 6.9 and 7.9
7
9
-
30% of optimal activity at pH 7 and pH 9
7.2
8.2
-
-
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
10
-
-
half maximal enzyme activity
PDB
SCOP
CATH
ORGANISM
Brucella abortus (strain 2308)
Brucella abortus (strain 2308)
Brucella abortus (strain 2308)
Brucella abortus (strain 2308)
Burkholderia pseudomallei (strain K96243)
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139)
Escherichia coli (strain K12)
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
no modification
-
non-covalently bound carbohydrate
side-chain modification
-
2% neutral sugar
phosphoprotein
-, Q5YLB6
inactivation by phosphorylation
no modification
-
-
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6
7
Candida brassicae
-
stable
7.5
9
-
at 15°C stable for 30 min
OXIDATION STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
reducing agent necessary for maintenance of enzyme activity
Lupinus sp.
-
33131
dithiothreitol very effective in protecting enzyme from loss of activity
-
33124
H2O2 inactivates isocitrate lyase and degrades its product glyoxylate. Gel filtration, cross-linking and co-immunoprecipitation studies indicate association of isocitrate lyase with catalase, this would afford protection from H2O2.
-
663767