Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | increase of activity | Aspergillus nidulans | |
dithiothreitol | optimal concentration 1 mM | Aspergillus nidulans | |
thiol compounds | increase of activity | Aspergillus nidulans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
fructose-1,6-bisphosphate | - |
Aspergillus nidulans | |
malonate | - |
Aspergillus nidulans | |
methylenesuccinate | - |
Aspergillus nidulans | |
oxalate | - |
Aspergillus nidulans | |
succinate | - |
Aspergillus nidulans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
threo-Ds-isocitrate | - |
Aspergillus nidulans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | no activity in absence of exogenous cation | Aspergillus nidulans | |
Co2+ | Co2+ 18% as effective as Mg2+ | Aspergillus nidulans | |
Mg2+ | required for full activity, optimal concentration 5 mM | Aspergillus nidulans | |
Mn2+ | no activity in absence of exogenous cation | Aspergillus nidulans | |
Mn2+ | Mn2+ 31% as effective as Mg2+ | Aspergillus nidulans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59000 | - |
4 * 59000, SDS-PAGE | Aspergillus nidulans |
240000 | - |
gel filtration | Aspergillus nidulans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate | Aspergillus nidulans | glyoxylate cycle | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus nidulans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Aspergillus nidulans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
11 | - |
- |
Aspergillus nidulans |
Storage Stability | Organism |
---|---|
-20°C, several weeks, stable | Aspergillus nidulans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R,3S)-isocitrate | - |
Aspergillus nidulans | succinate + glyoxylate | - |
? | |
isocitrate | - |
Aspergillus nidulans | succinate + glyoxylate | - |
r | |
isocitrate | glyoxylate cycle | Aspergillus nidulans | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 59000, SDS-PAGE | Aspergillus nidulans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 50 | stable for 1 h | Aspergillus nidulans |
50 | - |
completely inactive at temperatures above 50°C | Aspergillus nidulans |