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Literature summary for 4.1.3.1 extracted from
- Physicochemical and kinetic characteristics of isoforms of isocitrate lyase from corn (2009), Biochemistry (Moscow), 74, 528-532.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.056 | - |
isocitrate | ICL1 | Zea mays |  |
| 0.083 | - |
isocitrate | ICL2 | Zea mays | |
| 0.1 | - |
isocitrate | ICL | Zea mays | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | ICL1, dependent on | Zea mays | |
| Mn2+ | ICL2 and ICL, dependent on | Zea mays | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 164000 | - |
gel filtration, ICL1 | Zea mays |
| 207000 | - |
gel filtration, ICL2 | Zea mays |
| 208000 | - |
gel filtration, ICL | Zea mays |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Zea mays | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, purification of ICL1 is 123fold with a yield of 5.7%, purification degree and yield of ICL2 are 110 and 5.7%, respectively | Zea mays |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | ICL | Zea mays | - |
| scutellum | ICL1 and and ICL2 | Zea mays | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.04 | - |
homogenate | Zea mays |
| 4.4 | - |
ICL2, 1003fold purified | Zea mays |
| 4.9 | - |
ICL1, 123fold purified | Zea mays |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isocitrate | ICL1, ICL2 and ICL | Zea mays | succinate + glyoxylate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | gel filtration, ICL1, ICL2 and ICL | Zea mays |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ICL | - |
Zea mays |
| isocitrate lyase | - |
Zea mays |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
ICL2 | Zea mays |
| 7.5 | - |
ICL1 | Zea mays |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Zea mays | Ca2+, K+, and Na+ have little effect on the regulation of activity of ICL isoforms | additional information |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | participation of isoforms in metabolic regulation of the glyoxylate cycle, organic acid metabolism during photorespiration in leaves and acidosis in corn seeds | Zea mays |
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