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Literature summary for 4.1.3.1 extracted from
- Purification and characterization of a cold-adapted isocitrate lyase and a malate synthase from Colwellia maris, a psychrophilic bacterium (2001), Biosci. Biotechnol. Biochem., 65, 1095-1103.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.51 | - |
DL-isocitrate | 20°C, pH 6.8 | Colwellia maris |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | can partly replace Mg2+ | Colwellia maris | |
| Mg2+ | 10 mM, dependent | Colwellia maris | |
| Mn2+ | can partly replace Mg2+ | Colwellia maris | |
| Ni2+ | can partly replace Mg2+ | Colwellia maris | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 64700 | - |
4 * 64700, SDS-PAGE | Colwellia maris |
| 240000 | - |
gel filtration | Colwellia maris |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isocitrate | Colwellia maris | - |
succinate + glyoxylate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Colwellia maris | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Colwellia maris |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 6.17 | - |
- |
Colwellia maris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isocitrate | - |
Colwellia maris | succinate + glyoxylate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 64700, SDS-PAGE | Colwellia maris |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
- |
Colwellia maris |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | - |
half maximal enzyme activity | Colwellia maris |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
enzyme is rapidly inactivated above, NaCl, 10% glycerol and 1 mM dithiothreitol protects | Colwellia maris |
| 35 | - |
no enzyme activity | Colwellia maris |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 18.1 | - |
DL-isocitrate | 20°C, pH 6.8 | Colwellia maris | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
- |
Colwellia maris |
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