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Literature summary for 4.1.3.1 extracted from
- Purification and some properties of isocitrate lyase from the pollen of Pinus densiflora Sieb. et Zucc. (1986), Agric. Biol. Chem., 50, 409-416.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| extremely unstable in absence of Mg2+ or EDTA, glycerol or sucrose could not stabilize | Pinus densiflora |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | - |
Pinus densiflora |  |
| 3-phosphoglycerate | 29% inhibition at 1 mM | Pinus densiflora | |
| ADP | 20% inhibition at 2 mM | Pinus densiflora | |
| ATP | 35% inhibition at 2 mM | Pinus densiflora | |
| CoCl2 | - |
Pinus densiflora | |
| CuCl2 | - |
Pinus densiflora | |
| cysteine | - |
Pinus densiflora | |
| dithiothreitol | - |
Pinus densiflora | |
| FeCl2 | - |
Pinus densiflora | |
| glutathione | - |
Pinus densiflora | |
| Maleate | 66% inhibition at 0.5 mM | Pinus densiflora | |
| methylenesuccinate | 87% inhibition at 0.03 mM | Pinus densiflora | |
| oxalate | 71% inhibition at 0.5 mM | Pinus densiflora | |
| p-chloromercuribenzoate | reversible by cysteine | Pinus densiflora | |
| PbCl2 | - |
Pinus densiflora | |
| phosphoenolpyruvate | 53% inhibition at 1 mM | Pinus densiflora | |
| succinate | 7% inhibition at 0.5 mM | Pinus densiflora | |
| sulfhydryl compounds | - |
Pinus densiflora | |
| Tartrate | 33% inhibition at 0.5 mM | Pinus densiflora | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | Ba2+ less effective activator than Mg2+ | Pinus densiflora | |
| Mg2+ | essential for activity, optimal concentration 3 mM | Pinus densiflora | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65000 | - |
3 * 65000, SDS-PAGE | Pinus densiflora |
| 200000 | - |
gel filtration, glycerol density gradient | Pinus densiflora |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pinus densiflora | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pinus densiflora |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| pollen | - |
Pinus densiflora | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isocitrate | - |
Pinus densiflora | succinate + glyoxylate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 3 * 65000, SDS-PAGE | Pinus densiflora |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Pinus densiflora |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
more heat labile at low concentrations | Pinus densiflora |
| 50 | - |
completely inactivated after 5 min | Pinus densiflora |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
- |
Pinus densiflora |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | 8.2 | - |
Pinus densiflora |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8.5 | - |
Pinus densiflora |
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