Activating Compound | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | increases the substrate binding affinities isocitrate and succinate, but had little effect on the affinity for glyoxylate | Euglena gracilis |
Cloned (Comment) | Organism |
---|---|
His-tagged version (without signal peptide (amino acid residue 1-12)) expressed in Escherichia coli BL21(DE3) | Euglena gracilis |
Protein Variants | Comment | Organism |
---|---|---|
D475N | putative acetyl-CoA binding site of malate synthase, no malate synthase activity, but isocitrate lyase activity | Euglena gracilis |
additional information | truncated mutants: 13-573 (no isocitrate lyase activity, malate synthase activity), 566-1165 (no malate synthase activity), 20-573 (no isocitrate lyase activity, malate synthase activity), 38-573 (no isocitrate lyase activity), 20-1165 (isocitrate lyase activity and malate synthase activity), 38-1165 | Euglena gracilis |
R187K | putative acetyl-CoA binding site of malate synthase, no malate synthase activity, but isocitrate lyase activity | Euglena gracilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.96 | - |
glyoxylate | D475N mutant protein, 0.15 mM acetyl-CoA, 10 mM succinate, pH 6.5, 30°C | Euglena gracilis | |
1.3 | - |
glyoxylate | D475N mutant protein, 10 mM succinate, pH 6.5, 30°C | Euglena gracilis | |
6 | - |
succinate | D475N mutant protein, 1.5 mM glyoxylate, sharply decreased with increasing acetyl-CoA concentration, pH 6.5, 30°C | Euglena gracilis | |
6.7 | - |
isocitrate | when acetyl-CoA is present at concentrations higher than 5 mM, the Km value for isocitrate decreased by about one-third and the Vmax value increased about 2-fold, pH not specified in the publication, temperature not specified in the publication | Euglena gracilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
131000 | - |
4 * 131000, gel filtration, calculated subunit molecular mass | Euglena gracilis |
530000 | - |
gel filtration | Euglena gracilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Euglena gracilis | Q8LPA6 | - |
- |
Purification (Comment) | Organism |
---|---|
anion exchange chromatography (DEAE), immobilized metal ion affinity chromatography (Ni2+), gel filtration | Euglena gracilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate | - |
Euglena gracilis | succinate + glyoxylate | - |
r |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 131000, gel filtration, calculated subunit molecular mass | Euglena gracilis |
Synonyms | Comment | Organism |
---|---|---|
isocitrate lyase | activity of a bifunctional protein named glyoxylate cycle enzyme (malate synthase and isocitrate lyase activity) | Euglena gracilis |