Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | no inhibition | Zea mays | |
Ca2+ | no inhibition | Zea mays | |
Sr2+ | no inhibition | Zea mays |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
isocitrate | 100 mM Hepes, pH 7.0 | Zea mays | |
0.52 | - |
isocitrate | 60 mM potassium phosphate, pH 7.5 | Zea mays | |
0.76 | - |
isocitrate | 60 mM triethanolamine, pH 7.5 | Zea mays |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | no activation | Zea mays | |
Mg2+ | activity of demetallized enzyme: 0.5% | Zea mays | |
Mn2+ | Mn2+ 30% as effective as Mg2+ | Zea mays | |
Sr2+ | no activation | Zea mays |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
64000 | - |
4 * 64000, SDS-PAGE | Zea mays |
252000 | - |
gel filtration | Zea mays |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate | Zea mays | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
no modification | - |
Zea mays |
Purification (Comment) | Organism |
---|---|
- |
Zea mays |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
scutellum | - |
Zea mays | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.96 | - |
- |
Zea mays |
Storage Stability | Organism |
---|---|
-70°C, concentrated to 2.4 mg/ml, 10 mM triethanolamine buffer, 12 mM MgCl2, 14 mM 2-mercaptoethanol, pH 7.6, 50% v/v glycerol, several months, stable | Zea mays |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate | - |
Zea mays | succinate + glyoxylate | - |
r | |
isocitrate | - |
Zea mays | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 64000, SDS-PAGE | Zea mays |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
- |
Zea mays |