Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
vapour diffusion with 0.1 M Tris-HCl, 0.2 M sodium acetate, 20-30% (w/v) PEG 4000, pH 8.0 | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
C191A | unable to form the closed conformation crystal form | Mycobacterium tuberculosis |
C195A | reduced activity | Mycobacterium tuberculosis |
C195S | reduced activity | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.188 | - |
isocitrate | ICL1, in 50 mM MOPS pH 6.8, 5 mM MgCl2 | Mycobacterium tuberculosis | |
0.718 | - |
(2R,3S:2S,3R)-2-methyl-isocitrate | ICL1, in 50 mM MOPS pH 6.8, 5 mM MgCl2 | Mycobacterium tuberculosis | |
1.14 | - |
isocitrate | ICL2, in Tricine-HCl pH 7.5, 5 mM MgCl2 | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WKK7 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WKK7 | - |
- |
Purification (Comment) | Organism |
---|---|
HiTrap Q FF ion exchange column chromatography, Superdex S-200 gel filtration, and HiTrap Q HP ion exchange column chromatography | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R,3S:2S,3R)-2-methyl-isocitrate | - |
Mycobacterium tuberculosis | succinate + pyruvate | - |
? | |
(2R,3S:2S,3R)-2-methyl-isocitrate | - |
Mycobacterium tuberculosis H37Rv | succinate + pyruvate | - |
? | |
isocitrate | - |
Mycobacterium tuberculosis | succinate + glyoxylate | - |
? | |
isocitrate | - |
Mycobacterium tuberculosis H37Rv | succinate + glyoxylate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | x-ray crystallography | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
ICL1 | isoenzyme, has both isocitrate lyase and 2-methylisocitrate lyase activity | Mycobacterium tuberculosis |
ICL2 | isoenzyme, has no 2-methylisocitrate lyase activity | Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.25 | - |
(2R,3S:2S,3R)-2-methyl-isocitrate | ICL1, in 50 mM MOPS pH 6.8, 5 mM MgCl2 | Mycobacterium tuberculosis | |
1.38 | - |
isocitrate | ICL2, in Tricine-HCl pH 7.5, 5 mM MgCl2 | Mycobacterium tuberculosis | |
5.24 | - |
isocitrate | ICL1, in 50 mM MOPS pH 6.8, 5 mM MgCl2 | Mycobacterium tuberculosis |