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2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-triphosphate + H2O
?
2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone triphosphate + 2 H2O
formate + 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate + diphosphate
2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone triphosphate + H2O
2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate + diphosphate
-
formamide-type intermediate analogue
-
-
?
8-oxo-dGTP + H2O
?
-
-
-
-
?
dGTP + H2O
formate + 2,5-diamino-6-hydroxy-4-(5-phospho-deoxyribosylamino)pyrimidine + diphosphate
-
-
-
-
?
GTP + 3 H2O
formate + 2,5-diamino-6-beta-ribosylamino-4(3H)-pyrimidinone 5'-phosphate + diphosphate
GTP + 3 H2O
formate + 2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + diphosphate
GTP + H2O
2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate + diphosphate + ?
enzyme catalyzes the first step in the biosynthesis of riboflavin
-
-
?
GTP + H2O
2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate + diphosphate + ?
GTP + H2O
formate + 2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + diphosphate
GTP + H2O
formate + 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + diphosphate
GTP + H2O
formate + 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)pyrimidine + diphosphate
GTP + H2O
formate + 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate + diphosphate
additional information
?
-
2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-triphosphate + H2O
?
-
-
-
-
?
2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-triphosphate + H2O
?
-
-
-
?
2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone triphosphate + 2 H2O
formate + 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate + diphosphate
-
compound does serve as a substrate but not as kinetically competent intermediate
-
-
?
2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone triphosphate + 2 H2O
formate + 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate + diphosphate
-
formamide-type intermediate analogue
-
-
?
GTP + 3 H2O
formate + 2,5-diamino-6-beta-ribosylamino-4(3H)-pyrimidinone 5'-phosphate + diphosphate
-
-
spontaneous isomerization into the alpha-isomer, also the product can easily spontaneously decompose under formation of 2,4,5-triamino-4-(3H)-pyrimidone
-
?
GTP + 3 H2O
formate + 2,5-diamino-6-beta-ribosylamino-4(3H)-pyrimidinone 5'-phosphate + diphosphate
-
first committed step in the biosynthesis of riboflavin, overview
-
-
?
GTP + 3 H2O
formate + 2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + diphosphate
-
-
-
?
GTP + 3 H2O
formate + 2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + diphosphate
-
-
-
?
GTP + H2O
2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate + diphosphate + ?
-
-
-
?
GTP + H2O
2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate + diphosphate + ?
-
-
the reaction product is exclusively formed by enzyme variant SCO 6655
-
?
GTP + H2O
formate + 2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + diphosphate
-
GMP is a second reaction product of GTP cyclohydrolase II. The ratio of the main product, 2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine and GMP is produced at an approximate ratio of 10:1
-
?
GTP + H2O
formate + 2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + diphosphate
-
-
-
?
GTP + H2O
formate + 2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + diphosphate
-
-
-
?
GTP + H2O
formate + 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + diphosphate
-
-
-
-
?
GTP + H2O
formate + 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + diphosphate
-
-
-
-
?
GTP + H2O
formate + 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + diphosphate
-
-
-
-
?
GTP + H2O
formate + 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + diphosphate
-
-
-
-
?
GTP + H2O
formate + 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)pyrimidine + diphosphate
-
-
-
-
?
GTP + H2O
formate + 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)pyrimidine + diphosphate
-
-
-
?
GTP + H2O
formate + 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)pyrimidine + diphosphate
-
-
-
?
GTP + H2O
formate + 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)pyrimidine + diphosphate
-
-
-
-
?
GTP + H2O
formate + 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)pyrimidine + diphosphate
-
first committed step in the biosynthesis of riboflavin, overview
-
-
?
GTP + H2O
formate + 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate + diphosphate
-
-
GMP is also formed as a minor product
-
?
GTP + H2O
formate + 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate + diphosphate
-
first committed step in the biosynthesis of riboflavin, overview
-
-
?
GTP + H2O
formate + 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate + diphosphate
-
-
-
-
?
additional information
?
-
-
RibA is the rate limiting enzyme in an industrial riboflavin producing strain
-
-
?
additional information
?
-
-
RibA is a bifunctional enzyme possessing 3,4-dihydroxy-2-butanone 4-phosphate synthase activity, EC 4.1.99.12, located in the N-terminal half of the protein and GTP cyclohydrolase II activity of the C-terminal domain, overview
-
-
?
additional information
?
-
GTP cyclohydrolase II is a slow GTPase
-
-
?
additional information
?
-
enzyme may be involved in the biosynthesis of toxoflavin, which is produced from GTP in a series of steps that are likely initiated by GCH II
-
-
?
additional information
?
-
enzyme may be involved in the biosynthesis of toxoflavin, which is produced from GTP in a series of steps that are likely initiated by GCH II
-
-
?
additional information
?
-
enzyme may be involved in the biosynthesis of toxoflavin, which is produced from GTP in a series of steps that are likely initiated by GCH II
-
-
?
additional information
?
-
-
enzyme may be involved in the biosynthesis of toxoflavin, which is produced from GTP in a series of steps that are likely initiated by GCH II
-
-
?
additional information
?
-
substitution of Met with His leads to a protein that catalyzes the production of both 2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate and 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate with equal efficiency
-
-
?
additional information
?
-
substitution of Met with His leads to a protein that catalyzes the production of both 2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate and 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate with equal efficiency
-
-
?
additional information
?
-
substitution of Met with His leads to a protein that catalyzes the production of both 2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate and 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate with equal efficiency
-
-
?
additional information
?
-
-
substitution of Met with His leads to a protein that catalyzes the production of both 2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate and 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate with equal efficiency
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
GTP + 3 H2O
formate + 2,5-diamino-6-beta-ribosylamino-4(3H)-pyrimidinone 5'-phosphate + diphosphate
-
first committed step in the biosynthesis of riboflavin, overview
-
-
?
GTP + 3 H2O
formate + 2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + diphosphate
GTP + H2O
2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate + diphosphate + ?
enzyme catalyzes the first step in the biosynthesis of riboflavin
-
-
?
GTP + H2O
2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate + diphosphate + ?
-
-
-
?
GTP + H2O
formate + 2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + diphosphate
GTP + H2O
formate + 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + diphosphate
GTP + H2O
formate + 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)pyrimidine + diphosphate
GTP + H2O
formate + 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate + diphosphate
-
first committed step in the biosynthesis of riboflavin, overview
-
-
?
additional information
?
-
GTP + 3 H2O
formate + 2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + diphosphate
-
-
-
?
GTP + 3 H2O
formate + 2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + diphosphate
-
-
-
?
GTP + H2O
formate + 2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + diphosphate
-
GMP is a second reaction product of GTP cyclohydrolase II. The ratio of the main product, 2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine and GMP is produced at an approximate ratio of 10:1
-
?
GTP + H2O
formate + 2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + diphosphate
-
-
-
?
GTP + H2O
formate + 2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + diphosphate
-
-
-
?
GTP + H2O
formate + 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + diphosphate
-
-
-
-
?
GTP + H2O
formate + 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + diphosphate
-
-
-
-
?
GTP + H2O
formate + 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + diphosphate
-
-
-
-
?
GTP + H2O
formate + 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + diphosphate
-
-
-
-
?
GTP + H2O
formate + 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)pyrimidine + diphosphate
-
-
-
-
?
GTP + H2O
formate + 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)pyrimidine + diphosphate
-
first committed step in the biosynthesis of riboflavin, overview
-
-
?
additional information
?
-
-
RibA is the rate limiting enzyme in an industrial riboflavin producing strain
-
-
?
additional information
?
-
GTP cyclohydrolase II is a slow GTPase
-
-
?
additional information
?
-
enzyme may be involved in the biosynthesis of toxoflavin, which is produced from GTP in a series of steps that are likely initiated by GCH II
-
-
?
additional information
?
-
enzyme may be involved in the biosynthesis of toxoflavin, which is produced from GTP in a series of steps that are likely initiated by GCH II
-
-
?
additional information
?
-
enzyme may be involved in the biosynthesis of toxoflavin, which is produced from GTP in a series of steps that are likely initiated by GCH II
-
-
?
additional information
?
-
-
enzyme may be involved in the biosynthesis of toxoflavin, which is produced from GTP in a series of steps that are likely initiated by GCH II
-
-
?
additional information
?
-
substitution of Met with His leads to a protein that catalyzes the production of both 2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate and 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate with equal efficiency
-
-
?
additional information
?
-
substitution of Met with His leads to a protein that catalyzes the production of both 2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate and 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate with equal efficiency
-
-
?
additional information
?
-
substitution of Met with His leads to a protein that catalyzes the production of both 2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate and 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate with equal efficiency
-
-
?
additional information
?
-
-
substitution of Met with His leads to a protein that catalyzes the production of both 2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate and 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate with equal efficiency
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.031 - 0.122
2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-triphosphate
additional information
additional information
-
0.031
2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-triphosphate
-
wild type enzyme, at pH 8.5 and 30°C
0.079
2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-triphosphate
-
mutant enzyme Y210C/A290T/Q293R/A296T/K322R/M361I, at pH 8.5 and 30°C
0.122
2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-triphosphate
-
mutant enzyme Y210C/A290T/Q293R/A296T/K322R/F339Y/M361I, at pH 8.5 and 30°C
0.008
GTP
M120Y variant, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol
0.008
GTP
variant M120Y, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol
0.0089
GTP
M120K variant, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol
0.01
GTP
-
wild type enzyme, at pH 8.5 and 30°C
0.011
GTP
M120Q variant, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol
0.015
GTP
M120I variant, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol
0.019
GTP
M120F variant, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol
0.019
GTP
variant M120F, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol
0.024
GTP
wild type, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol
0.033
GTP
M120S variant, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol
0.044
GTP
-
mutant enzyme Y210C/A290T/Q293R/A296T/K322R/F339Y/M361I, at pH 8.5 and 30°C
0.048
GTP
variant Y123M, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol
0.049
GTP
-
mutant enzyme Y210C/A290T/Q293R/A296T/K322R/M361I, at pH 8.5 and 30°C
0.05
GTP
M120H variant, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol
0.056
GTP
wild type, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol
0.059
GTP
M120A variant, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol
0.063
GTP
wild type, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol
0.067
GTP
M120C variant, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol
0.067
GTP
M120N variant, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol
0.085
GTP
variant D127A, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol
0.092
GTP
variant R123M, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol
0.1267
GTP
full-length bifunctional enzyme ribA2, at pH 8.0 and 37°C
0.15
GTP
M120T variant, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol
0.1505
GTP
GCHII-domain of enzyme ribA2, at pH 8.0 and 37°C
0.19
GTP
M120G variant, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol
0.23
GTP
variant G209D, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol
additional information
additional information
-
kinetics, cooperative reaction with a Hill coefficient of 1.3
-
additional information
additional information
-
pre-steady state kinetics, single turnover experiments, stopped-flow experiments
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.05 - 0.1
2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-triphosphate
0.05
2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-triphosphate
-
wild type enzyme, at pH 8.5 and 30°C
0.09
2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-triphosphate
-
mutant enzyme Y210C/A290T/Q293R/A296T/K322R/M361I, at pH 8.5 and 30°C
0.1
2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-triphosphate
-
mutant enzyme Y210C/A290T/Q293R/A296T/K322R/F339Y/M361I, at pH 8.5 and 30°C
0.0033
GTP
full-length bifunctional enzyme ribA2, at pH 8.0 and 37°C
0.005
GTP
GCHII-domain of enzyme ribA2, at pH 8.0 and 37°C
0.028
GTP
-
recombinant enzyme
0.035
GTP
-
wild type enzyme, at pH 8.5 and 30°C
0.065
GTP
-
mutant enzyme Y210C/A290T/Q293R/A296T/K322R/M361I, at pH 8.5 and 30°C
0.072
GTP
-
mutant enzyme Y210C/A290T/Q293R/A296T/K322R/F339Y/M361I, at pH 8.5 and 30°C
0.42
GTP
M120D variant, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol
1.2
GTP
variant Y326F, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol
1.8
GTP
M120W variant, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol
3.18
GTP
M120E variant, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol
3.18
GTP
SCO 2687, variant Y123F, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol
4.86
GTP
variant Y326M, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol
10.8
GTP
M120H variant, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol
20.4
GTP
M120T variant, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol
42
GTP
M120A variant, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol
46.8
GTP
variant M120F, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol
57
GTP
M120G variant, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol
66
GTP
M120C variant, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol
78
GTP
M120I variant, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol
78
GTP
SCO 2687, variant D127A, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol
84
GTP
M120S variant, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol
84
GTP
variant M120Y, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol
108
GTP
M120N variant, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol
120
GTP
M120Q variant, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol
150
GTP
wild type, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol
168
GTP
SCO 2687, variant G209D, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol
204
GTP
SCO 2687, wild type, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol
228
GTP
SCO 2687, variant R123M, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol
348
GTP
SCO 2687, variant Y123M, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol
432
GTP
wild type, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol
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physiological function
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3,4-dihydroxy 2-butanone 4-phosphate synthase, DHBPS EC 4.1.99.12, and GTP cyclohydrolase-II, GTPCH-II, are the two initial enzymes involved in riboflavin biosynthesis pathway, which is essential for the pathogen
additional information
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bifunctional enzyme with 3,4-dihydroxy 2-butanone 4-phosphate synthase, DHBPS EC 4.1.99.12, and GTP cyclohydrolase-II, GTPCH-II, domains at N- and C-termini, respectively
malfunction
NbRibA-silenced plants show slightly dwarf phenotypes, levels of endogenous riboflavin and its derivatives FMN and FAD are decreased in NbRibA-silenced Nicotiana benthamiana plants. Silencing of NbRibA compromises not only hypersensitive response cell death, but also the NO and reactive oxygen species production induced by INF1 elicitin and a constitutively active form of NbMEK2, and also induces high susceptibility to oomycete Phytophthora infestans and ascomycete Colletotrichum orbiculare. Compromised radical production and hypersensitive response cell death induced by INF1 in NbRibA-silenced leaves are rescued by adding riboflavin, FMN or FAD
malfunction
pigmentation defect due to mutation of gch2
metabolism
GCH II catalyzes the first committed step in the riboflavin biosynthetic pathway
metabolism
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GTP cyclohydrolase II catalyzes the first dedicated step in the biosynthesis of riboflavin
metabolism
-
3,4-dihydroxy 2-butanone 4-phosphate synthase, DHBPS EC 4.1.99.12, and GTP cyclohydrolase-II, GTPCH-II, are the two initial enzymes involved in riboflavin biosynthesis pathway, which is essential for the pathogen
metabolism
the bifunctional enzyme, guanosine triphosphate cyclohydrolase II/3,4-dihydroxy-2-butanone-4-phosphate synthase, participates in the biosynthesis of flavin
metabolism
GTP cyclohydrolase II catalyzes the first committed step in the biosynthesis of riboflavin
metabolism
critical protein in the pteridine biosynthetic pathway
metabolism
the enzyme is essential for pathogens. The enzyme can regulate its catalytic activity depending on the redox environment
metabolism
the enzyme is involved in riboflavin biosynthesis and in biosynthesis of toxoflavin (a phytotoxin that contributes the virulence of this phytopathogen)
metabolism
-
GCH II catalyzes the first committed step in the riboflavin biosynthetic pathway
-
metabolism
-
the enzyme is essential for pathogens. The enzyme can regulate its catalytic activity depending on the redox environment
-
metabolism
-
the enzyme is involved in riboflavin biosynthesis and in biosynthesis of toxoflavin (a phytotoxin that contributes the virulence of this phytopathogen)
-
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Y210C/A290T/Q293R/A296T/K322R/F339Y/M361I
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the mutant shows a 2fold increase in GTP cyclohydrolase II activity and a 4fold increase in the Km value with GTP as the substrate. Using 2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-triphosphate as the substrate, the mutant shows a rate enhancement by a factor of about 2 and an increase in the Km value by a factor of about 5
Y210C/A290T/Q293R/A296T/K322R/M361I
-
the mutant shows increased kcat and Km values compared to the wild type enzyme using GTP and 2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-triphosphate as the substrate
C54S
-
site-directed mutagenesis, mutation results in proteins devoid of bound zinc and unable to release formate from the imidazole ring of GTP or from the intermediate analogue 2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-triphosphate, however, the mutant enzyme is still capable to release diphosphate from GTP and from the formamide-type intermediate analogue
C65S
-
site-directed mutagenesis, mutation results in proteins devoid of bound zinc and unable to release formate from the imidazole ring of GTP or from the intermediate analogue 2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-triphosphate, however, the mutant enzyme is still capable to release diphosphate from GTP and from the formamide-type intermediate analogue
C67S
-
site-directed mutagenesis, mutation results in proteins devoid of bound zinc and unable to release formate from the imidazole ring of GTP or from the intermediate analogue 2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-triphosphate, however, the mutant enzyme is still capable to release diphosphate from GTP and from the formamide-type intermediate analogue
C396S
site-directed mutagenesis,
E154D
site-directed mutagenesis,
D127A
about 10fold less efficient than the wild-type protein
G209D
about 10fold less efficient than the wild-type protein
M120A
generated by site-directed mutagenesis
M120C
generated by site-directed mutagenesis
M120D
generated by site-directed mutagenesis
M120E
generated by site-directed mutagenesis
M120F
generated by site-directed mutagenesis
M120G
generated by site-directed mutagenesis
M120H
generated by site-directed mutagenesis
M120I
generated by site-directed mutagenesis
M120K
generated by site-directed mutagenesis
M120N
generated by site-directed mutagenesis
M120Q
generated by site-directed mutagenesis
M120S
generated by site-directed mutagenesis
M120T
generated by site-directed mutagenesis
M120W
generated by site-directed mutagenesis
M120Y
generated by site-directed mutagenesis
R83H
mutant substantially impaired in overall activity
Y123M
about 10fold less efficient than the wild-type protein, produces exclusively 2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate
Y326F
generated by site-directed mutagenesis, catalytically inactive variant
Y326M
generated by site-directed mutagenesis, catalytically inactive variant
additional information
-
an additional single copy of the ribA gene introduced into the sacBlocus of the riboflavin production strain and constitutive expression from the medium strength vegI promoter leads to improved riboflavin titers and yields of riboflavin on glucose of up to 25%, strain VB2XL1, both enzymatic activities of RibA, the 3,4-dihydroxy-2-butanone 4-phosphate synthase activity located in the N-terminal half of the protein and the GTP cyclohydrolase II activity of the C-terminal domain, are necessary for the improved riboflavin productivity, method, overview
additional information
generation of NbRibA-silenced plants via inoculation with Agrobacterium tumefaciens containing inf1, NbMEK2DD or Bax, or GUS as a control. Silencing NbRibA does not affect the activation of SIPK and defense-related genes, except for NbGST, effects of silencing NbRibA on disease resistance, phenotype
additional information
-
generation of NbRibA-silenced plants via inoculation with Agrobacterium tumefaciens containing inf1, NbMEK2DD or Bax, or GUS as a control. Silencing NbRibA does not affect the activation of SIPK and defense-related genes, except for NbGST, effects of silencing NbRibA on disease resistance, phenotype
additional information
-
the M120T variant of GCH II/II SCO 6655 acquires the ability to catalyze the conversion of GTP to 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate
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