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EC Number
Crystallization (Commentary)
3.5.4.25
selenomethionine-labeled crystals, 1.54 A resolution
3.5.4.25
sitting drop vapor diffusion method, using 0.1 M Na HEPES pH 7.5, 15% (w/v) PEG 8000
3.5.4.25
the enzyme is crystallized in oxidized form, the expulsion of Zn(2+) upon oxidation of catalytic cysteines reveales its ability to act in response to the redox environment. The crystal structure of Helicobacter pylori, unlike other GCHII structures, reveales that cysteines at the active site exist in oxidized state forming two disulfide bonds
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