Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.4.25 extracted from

  • Lehmann, M.; Degen, S.; Hohmann, H.P.; Wyss, M.; Bacher, A.; Schramek, N.
    Biosynthesis of riboflavin. Screening for an improved GTP cyclohydrolase II mutant (2009), FEBS J., 276, 4119-4129.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
Y210C/A290T/Q293R/A296T/K322R/F339Y/M361I the mutant shows a 2fold increase in GTP cyclohydrolase II activity and a 4fold increase in the Km value with GTP as the substrate. Using 2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-triphosphate as the substrate, the mutant shows a rate enhancement by a factor of about 2 and an increase in the Km value by a factor of about 5 Bacillus subtilis
Y210C/A290T/Q293R/A296T/K322R/M361I the mutant shows increased kcat and Km values compared to the wild type enzyme using GTP and 2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-triphosphate as the substrate Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
GTP wild type enzyme, at pH 8.5 and 30°C Bacillus subtilis
0.031
-
2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-triphosphate wild type enzyme, at pH 8.5 and 30°C Bacillus subtilis
0.044
-
GTP mutant enzyme Y210C/A290T/Q293R/A296T/K322R/F339Y/M361I, at pH 8.5 and 30°C Bacillus subtilis
0.049
-
GTP mutant enzyme Y210C/A290T/Q293R/A296T/K322R/M361I, at pH 8.5 and 30°C Bacillus subtilis
0.079
-
2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-triphosphate mutant enzyme Y210C/A290T/Q293R/A296T/K322R/M361I, at pH 8.5 and 30°C Bacillus subtilis
0.122
-
2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-triphosphate mutant enzyme Y210C/A290T/Q293R/A296T/K322R/F339Y/M361I, at pH 8.5 and 30°C Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-triphosphate + H2O
-
Bacillus subtilis ?
-
?
GTP + H2O
-
Bacillus subtilis formate + 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)pyrimidine + diphosphate
-
?

Synonyms

Synonyms Comment Organism
GCH II
-
Bacillus subtilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.035
-
GTP wild type enzyme, at pH 8.5 and 30°C Bacillus subtilis
0.05
-
2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-triphosphate wild type enzyme, at pH 8.5 and 30°C Bacillus subtilis
0.065
-
GTP mutant enzyme Y210C/A290T/Q293R/A296T/K322R/M361I, at pH 8.5 and 30°C Bacillus subtilis
0.072
-
GTP mutant enzyme Y210C/A290T/Q293R/A296T/K322R/F339Y/M361I, at pH 8.5 and 30°C Bacillus subtilis
0.09
-
2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-triphosphate mutant enzyme Y210C/A290T/Q293R/A296T/K322R/M361I, at pH 8.5 and 30°C Bacillus subtilis
0.1
-
2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-triphosphate mutant enzyme Y210C/A290T/Q293R/A296T/K322R/F339Y/M361I, at pH 8.5 and 30°C Bacillus subtilis

General Information

General Information Comment Organism
metabolism GTP cyclohydrolase II catalyzes the first dedicated step in the biosynthesis of riboflavin Bacillus subtilis