Protein Variants | Comment | Organism |
---|---|---|
Y210C/A290T/Q293R/A296T/K322R/F339Y/M361I | the mutant shows a 2fold increase in GTP cyclohydrolase II activity and a 4fold increase in the Km value with GTP as the substrate. Using 2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-triphosphate as the substrate, the mutant shows a rate enhancement by a factor of about 2 and an increase in the Km value by a factor of about 5 | Bacillus subtilis |
Y210C/A290T/Q293R/A296T/K322R/M361I | the mutant shows increased kcat and Km values compared to the wild type enzyme using GTP and 2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-triphosphate as the substrate | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
GTP | wild type enzyme, at pH 8.5 and 30°C | Bacillus subtilis | |
0.031 | - |
2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-triphosphate | wild type enzyme, at pH 8.5 and 30°C | Bacillus subtilis | |
0.044 | - |
GTP | mutant enzyme Y210C/A290T/Q293R/A296T/K322R/F339Y/M361I, at pH 8.5 and 30°C | Bacillus subtilis | |
0.049 | - |
GTP | mutant enzyme Y210C/A290T/Q293R/A296T/K322R/M361I, at pH 8.5 and 30°C | Bacillus subtilis | |
0.079 | - |
2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-triphosphate | mutant enzyme Y210C/A290T/Q293R/A296T/K322R/M361I, at pH 8.5 and 30°C | Bacillus subtilis | |
0.122 | - |
2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-triphosphate | mutant enzyme Y210C/A290T/Q293R/A296T/K322R/F339Y/M361I, at pH 8.5 and 30°C | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-triphosphate + H2O | - |
Bacillus subtilis | ? | - |
? | |
GTP + H2O | - |
Bacillus subtilis | formate + 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)pyrimidine + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GCH II | - |
Bacillus subtilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.035 | - |
GTP | wild type enzyme, at pH 8.5 and 30°C | Bacillus subtilis | |
0.05 | - |
2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-triphosphate | wild type enzyme, at pH 8.5 and 30°C | Bacillus subtilis | |
0.065 | - |
GTP | mutant enzyme Y210C/A290T/Q293R/A296T/K322R/M361I, at pH 8.5 and 30°C | Bacillus subtilis | |
0.072 | - |
GTP | mutant enzyme Y210C/A290T/Q293R/A296T/K322R/F339Y/M361I, at pH 8.5 and 30°C | Bacillus subtilis | |
0.09 | - |
2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-triphosphate | mutant enzyme Y210C/A290T/Q293R/A296T/K322R/M361I, at pH 8.5 and 30°C | Bacillus subtilis | |
0.1 | - |
2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-triphosphate | mutant enzyme Y210C/A290T/Q293R/A296T/K322R/F339Y/M361I, at pH 8.5 and 30°C | Bacillus subtilis |
General Information | Comment | Organism |
---|---|---|
metabolism | GTP cyclohydrolase II catalyzes the first dedicated step in the biosynthesis of riboflavin | Bacillus subtilis |