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Information on EC 3.1.11.3 - exodeoxyribonuclease (lambda-induced)
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3.1.11.3 exodeoxyribonuclease (lambda-induced)Specify your search results
Word Map
- 3.1.11.3
-
single-stranded
- strand
- endonuclease
- polymerases
-
proofreading
- mismatch
- duplex
- helicase
- bacteriophage
-
fidelity
-
exonucleolytic
- rnase
- nucleases
- ssdna
- nick
- dntp
-
werner
-
klenow
- fork
-
anneal
- polynucleotide
- exoribonuclease
- reca
-
unwind
- mre11
-
ultrasensitive
- aptamer
-
overhang
-
misincorporation
-
abasic
- mispairs
-
recbcd
-
translesion
-
apurinic
- deoxynucleoside
-
nucleolytic
-
deadenylation
- 3'-terminus
-
decapping
-
replisome
-
structure-specific
- ape1
-
template-primer
-
okazaki
-
aptasensor
- primase
-
primer-template
-
deadenylase
- analysis
- molecular biology
-
progeroid
- synthesis
-
5'-phosphorylated
The enzyme appears in viruses and cellular organisms
Reaction Schemes
exonucleolytic cleavage in the 5'- to 3'-direction to yield nucleoside 5'-phosphates
Synonyms
exonuclease, lambda exonuclease, lambda-exonuclease, 5'exonuclease, lambda exo, lambda-exo, phage lambda-induced exonuclease, 
more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
5'exonuclease
-
-
-
-
EC 3.1.4.28
-
-
formerly
-
exonuclease
-
-
-
-
lambda exonuclease
lambda exonuclease
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
exonucleolytic cleavage in the 5'- to 3'-direction to yield nucleoside 5'-phosphates
-
-
-
-
exonucleolytic cleavage in the 5'- to 3'-direction to yield nucleoside 5'-phosphates
lambda exonuclease uses a classic two-metal mechanism
Lambdavirus lambda
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of phosphoric ester
-
-
-
-
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CAS REGISTRY NUMBER
COMMENTARY
37288-28-1
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
GTTTTAAATTATGGAGTATGTCAVTGTGGAGACGAGAGTAAG + H2O
?
Lambdavirus lambda
a target sequence
-
-
?
GTTTTAAATTATGGAGTATGTGAVTGTGGAGACGAGAGTAAG + H2O
?
Lambdavirus lambda
a target sequence
-
-
?
GTTTTAAATTATGGAGTATGTGTCTGTGCAVACGAGAGTAAG + H2O
?
Lambdavirus lambda
a target sequence
-
-
?
GTTTTAAATTATGGAGTATGTGTCTGTGGAVACGAGAGTAAG + H2O
?
Lambdavirus lambda
a target sequence
-
-
?
GTTTTAAATTATGGAGTATGTGTCTGTGVAGACGAGAGTAAG + H2O
?
Lambdavirus lambda
a target sequence
-
-
?
PO4-TAT(-FAM)CCACAGACACATACTCCA-BHQ1 + H2O
?
Lambdavirus lambda
a probe sequence
-
-
?
PO4-TCTCCACAYT(-FAM)CACATACTCCA-BHQ1 + H2O
?
Lambdavirus lambda
a probe sequence, Y = tetrahydrofuran abasic site
-
-
?
PO4-TUT(-FAM)ACACAGACACATACTCCA-BHQ1 + H2O
?
Lambdavirus lambda
a probe sequence
-
-
?
PO4-TUT(-FAM)TCACAGACACATACTCCA-BHQ1 + H2O
?
Lambdavirus lambda
a probe sequence
-
-
?
PO4-TXT(-FAM)TCACAGACACATACTCCA-BHQ1 + H2O
?
Lambdavirus lambda
a probe sequence, X = natural abasic site generated by UDG
-
-
?
PO4-TYT(-FAM)ACACAGACACATACTCCA-BHQ1 + H2O
?
Lambdavirus lambda
a probe sequence, Y = tetrahydrofuran abasic site
-
-
?
PO4-TYT(-FAM)CCACAGACACATACTCCA-BHQ1 + H2O
?
Lambdavirus lambda
a probe sequence, Y = tetrahydrofuran abasic site
-
-
?
PO4-TYT(-FAM)TCACAGACACATACTCCA-BHQ1 + H2O
?
Lambdavirus lambda
a probe sequence, Y = tetrahydrofuran abasic site
-
-
?
PO4-TYTCCACAGACACAT(-FAM)ACTCCATAATTTAA-BHQ1 + H2O
?
Lambdavirus lambda
a probe sequence, Y = tetrahydrofuran abasic site
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
-
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
exonucleolytic cleavage in the 5'- to 3'-direction to yield 5'-phosphomononucleotides
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
degradation also at single-stranded breaks within DNA-duplex
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
possess "red" recombination system
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
high processivity
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
no activity on denatured DNA or RNA
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
degrades native DNA 100 times faster than long single-stranded DNA or oligonucleotides
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
shows strong preference for termini bearing 5'-phosphoryl groups
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
no attack at single-stranded breaks within DNA-duplex
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
degrades double-stranded native DNA
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
preference for a 5'-phosphoryl group over a 5'-hydroxyl group
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
lambda exonuclease is unable to initiate DNA digestion at nicks or gaps
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
-
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
degradation also at single-stranded breaks within DNA-duplex
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
degrades double-stranded native DNA
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
Lambdavirus lambda
-
-
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
Lambdavirus lambda
-
exonucleolytic cleavage in the 5'- to 3'-direction to yield 5'-phosphomononucleotides
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
Lambdavirus lambda
-
possess "red" recombination system
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
Lambdavirus lambda
-
high processivity
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
Lambdavirus lambda
-
no activity on denatured DNA or RNA
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
Lambdavirus lambda
-
degrades native DNA 100 times faster than long single-stranded DNA or oligonucleotides
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
Lambdavirus lambda
-
shows strong preference for termini bearing 5'-phosphoryl groups
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
Lambdavirus lambda
-
no attack at single-stranded breaks within DNA-duplex
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
Lambdavirus lambda
-
degrades double-stranded native DNA
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
Lambdavirus lambda
-
preference for a 5'-phosphoryl group over a 5'-hydroxyl group
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
Lambdavirus lambda phage 80
-
-
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
-
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
no activity on denatured DNA or RNA
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
degrades double-stranded native DNA
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
-
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
activity depends on lenght of double-stranded DNA
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-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
high processivity
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
degrades double-stranded native DNA
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
lambda exonuclease selectively digests the phosphorylated undesired strand of double-stranded DNA and produces single-stranded DNA in high yield and purity
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
sequence-dependent rate and pausing of individual lambda exonuclease enzymes during the digestion of a DNA sequence containing 120 CGG repeats. GC-rich repetitive sequences provide a significant barrier to lambda exonuclease activity by increasing the probability of pausing in addition to reducing the average digestion rate
-
-
?
oligonucleotides + H2O
5'-phosphomononucleotides
-
strong preference for relatively short chain oligonucleotides
-
-
?
oligonucleotides + H2O
5'-phosphomononucleotides
Lambdavirus lambda
-
strong preference for relatively short chain oligonucleotides
-
-
?
single-stranded DNA + H2O
5'-phosphomononucleotides
-
mechanism for stimulation of recombination
-
-
?
single-stranded DNA + H2O
5'-phosphomononucleotides
-
degrades the 5'-terminated strand at a single-stranded branch
-
-
?
single-stranded DNA + H2O
5'-phosphomononucleotides
-
rate of digestion and binding is inversely related to the chain lenght
-
-
?
single-stranded DNA + H2O
5'-phosphomononucleotides
-
digestion not processively
-
-
?
single-stranded DNA + H2O
5'-phosphomononucleotides
-
at single-stranded DNA branches
-
-
?
single-stranded DNA + H2O
5'-phosphomononucleotides
Lambdavirus lambda
-
mechanism for stimulation of recombination
-
-
?
single-stranded DNA + H2O
5'-phosphomononucleotides
Lambdavirus lambda
-
degrades the 5'-terminated strand at a single-stranded branch
-
-
?
single-stranded DNA + H2O
5'-phosphomononucleotides
Lambdavirus lambda
-
rate of digestion and binding is inversely related to the chain lenght
-
-
?
single-stranded DNA + H2O
5'-phosphomononucleotides
Lambdavirus lambda
-
digestion not processively
-
-
?
single-stranded DNA + H2O
5'-phosphomononucleotides
Lambdavirus lambda
-
binds to circular or linear single-stranded DNA
-
-
?
single-stranded DNA + H2O
5'-phosphomononucleotides
Lambdavirus lambda
-
at single-stranded DNA branches
-
-
?
single-stranded DNA + H2O
5'-phosphomononucleotides
-
degrades ssDNA at higher enzyme concentrations
-
-
?
additional information
?
-
Lambdavirus lambda
-
for enzyme digestion of single DNA molecules, individual lambda-DNA molecules labeled with the fluorescent dye, YOYO-1, are stretched in a laminar flow stream and immobilized on a bare fused-silica prism surface based on hydrophobic and electrostatic interactions. Enzyme digestion is initiated by the influx of lambda-exonuclease enzyme via capillary force, method evaluation, overview. Progression of single-DNA digestion by lambda-exonuclease enzyme based on the decrease in the length of the DNA molecule at a dye:bp ratio of 1:5
-
-
?
additional information
?
-
Lambdavirus lambda
-
the enzyme unwinds the DNA prior to cleavage, such that two nucleotides of the 5'-ended strand insert into the active site of one subunit of the trimer, while the 3'-ended strand passes through the central channel to emerge out the back of the trimer. Unwinding of the DNA is facilitated by several apolar residues, including Leu78, that wedge into the base pairs at the single/double-strand junction to form favorable hydrophobic interactions. The terminal 5' phosphate of the DNA binds to a positively charged pocket buried at the end of the active site, while the scissile phosphate bridges two active site Mg2+ ions, mechanism of progressivity, structure-activity analysis, overview
-
-
?
additional information
?
-
Lambdavirus lambda
G-quadruplexes are resistant to lambda-exo digestion
-
-
?
additional information
?
-
Lambdavirus lambda
an ultrasensitive electrochemical biosensor for polynucleotide kinase assay based on gold nanoparticle-mediated lambda exonuclease cleavage-induced signal amplification is constructed. The presence of polynucleotide kinase (PNK) induces the phosphorylation of the strand 2-strand 1 hybrid and the subsequent cleavage of double-stranded DNA (dsDNA) by lambda exonuclease, resulting in the release of AuNP-strand 2 conjugates and [Ru(NH3)6]3+ from the gold electrode surface and consequently the decrease of electrochemical signal
-
-
?
additional information
?
-
Lambdavirus lambda
analysis of the ability of enzyme lambda-exo to efficiently digest G4 structures by digesting a plasmid derived from the human MYC locus (from MCF-7 breast cancer cells) that contains a well-characterized G4 motif, overview. Genome-wide biases of lambda-exo are profiled by sequencing the DNA remaining after lambda-exo digestion of sonicated genomic DNA (gDNA) from nonreplicating G0 MCF7 cells (LexoG0). The enzyme does not efficiently digest G-quadruplex (G4) structures in a plasmid. And enzyme lambda-exo digestion of nonreplicating genomic DNA (LexoG0) enriches GC-rich DNA and G4 motifs genome-wide
-
-
?
additional information
?
-
Lambdavirus lambda
sensitive discrimination of stable mismatched base pairs by an abasic site modified fluorescent probe and lambda exonuclease, substrate is target ssDNA with diverse DNA sequences, overview. Substrate specificity for base positions, discrimination factors of C:C type mismatched probe/target duplex, discrimination factors (DF) for duplexes containing different types of mismatched base pairs at position +I by using the abasic site modified probe in the absence and presence of different blocker strands, tested blocker sequences for the targets and type of mismatched base pair, overview
-
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
additional information
?
-
Lambdavirus lambda
G-quadruplexes are resistant to lambda-exo digestion
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
-
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
-
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
Lambdavirus lambda
-
-
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
Lambdavirus lambda phage 80
-
-
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
-
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
-
-
-
?
single-stranded DNA + H2O
5'-phosphomononucleotides
-
at single-stranded DNA branches
-
-
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Mg2+
Mn2+
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
9.2 - 9.5
9.4
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7 - 10.2
7.5
9.6
7 - 10.2
-
pH 7.0: 16%, 8.0: 35%, pH 10.0: 35%, 10.2: 8% of maximal activity
7 - 10.2
Lambdavirus lambda
-
pH 7.0: 16%, 8.0: 35%, pH 10.0: 35%, 10.2: 8% of maximal activity
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Lambdavirus lambda