Application | Comment | Organism |
---|---|---|
analysis | an ultrasensitive electrochemical biosensor for polynucleotide kinase assay based on gold nanoparticle-mediated lambda exonuclease cleavage-induced signal amplification is constructed. The presence of polynucleotide kinase (PNK) induces the phosphorylation of the strand 2-strand 1 hybrid and the subsequent cleavage of double-stranded DNA (dsDNA) by lambda exonuclease, resulting in the release of AuNP-strand 2 conjugates and [Ru(NH3)6]3+ from the gold electrode surface and consequently the decrease of electrochemical signal. The PNK activity can be simply monitored by the measurement of [Ru(NH3)6]3+ peak current signal. This assay is very sensitive with a very low detection limit and exhibits a large dynamic range from 0.001 to 10 U/ml. The method can be used to screen the PNK inhibitors, and it shows excellent performance in real sample analysis, thus holding great potential for further applications in biological researches and clinic diagnosis | Lambdavirus lambda |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Lambdavirus lambda |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lambdavirus lambda | P03697 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | an ultrasensitive electrochemical biosensor for polynucleotide kinase assay based on gold nanoparticle-mediated lambda exonuclease cleavage-induced signal amplification is constructed. The presence of polynucleotide kinase (PNK) induces the phosphorylation of the strand 2-strand 1 hybrid and the subsequent cleavage of double-stranded DNA (dsDNA) by lambda exonuclease, resulting in the release of AuNP-strand 2 conjugates and [Ru(NH3)6]3+ from the gold electrode surface and consequently the decrease of electrochemical signal | Lambdavirus lambda | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
lambda exonuclease | - |
Lambdavirus lambda |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Lambdavirus lambda |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.4 | - |
assay at | Lambdavirus lambda |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Lambdavirus lambda |