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Literature summary for 3.1.11.3 extracted from

  • Cui, L.; Li, Y.; Lu, M.; Tang, B.; Zhang, C.Y.
    An ultrasensitive electrochemical biosensor for polynucleotide kinase assay based on gold nanoparticle-mediated lambda exonuclease cleavage-induced signal amplification (2018), Biosens. Bioelectron., 99, 1-7 .
    View publication on PubMed

Application

Application Comment Organism
analysis an ultrasensitive electrochemical biosensor for polynucleotide kinase assay based on gold nanoparticle-mediated lambda exonuclease cleavage-induced signal amplification is constructed. The presence of polynucleotide kinase (PNK) induces the phosphorylation of the strand 2-strand 1 hybrid and the subsequent cleavage of double-stranded DNA (dsDNA) by lambda exonuclease, resulting in the release of AuNP-strand 2 conjugates and [Ru(NH3)6]3+ from the gold electrode surface and consequently the decrease of electrochemical signal. The PNK activity can be simply monitored by the measurement of [Ru(NH3)6]3+ peak current signal. This assay is very sensitive with a very low detection limit and exhibits a large dynamic range from 0.001 to 10 U/ml. The method can be used to screen the PNK inhibitors, and it shows excellent performance in real sample analysis, thus holding great potential for further applications in biological researches and clinic diagnosis Lambdavirus lambda

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Lambdavirus lambda

Organism

Organism UniProt Comment Textmining
Lambdavirus lambda P03697
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information an ultrasensitive electrochemical biosensor for polynucleotide kinase assay based on gold nanoparticle-mediated lambda exonuclease cleavage-induced signal amplification is constructed. The presence of polynucleotide kinase (PNK) induces the phosphorylation of the strand 2-strand 1 hybrid and the subsequent cleavage of double-stranded DNA (dsDNA) by lambda exonuclease, resulting in the release of AuNP-strand 2 conjugates and [Ru(NH3)6]3+ from the gold electrode surface and consequently the decrease of electrochemical signal Lambdavirus lambda ?
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Synonyms

Synonyms Comment Organism
lambda exonuclease
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Lambdavirus lambda

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
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assay at Lambdavirus lambda

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.4
-
assay at Lambdavirus lambda

Cofactor

Cofactor Comment Organism Structure
ATP
-
Lambdavirus lambda