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Literature summary for 3.1.11.3 extracted from
- Digestion of individual DNA molecules by lambda-exonuclease at liquid-solid interface (2010), Analyst, 135, 1759-1764.
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | activates | Lambdavirus lambda |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lambdavirus lambda | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | for enzyme digestion of single DNA molecules, individual lambda-DNA molecules labeled with the fluorescent dye, YOYO-1, are stretched in a laminar flow stream and immobilized on a bare fused-silica prism surface based on hydrophobic and electrostatic interactions. Enzyme digestion is initiated by the influx of lambda-exonuclease enzyme via capillary force, method evaluation, overview. Progression of single-DNA digestion by lambda-exonuclease enzyme based on the decrease in the length of the DNA molecule at a dye:bp ratio of 1:5 | Lambdavirus lambda | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| lambda-exonuclease | - |
Lambdavirus lambda |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Lambdavirus lambda |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.4 | - |
assay at | Lambdavirus lambda |
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