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Literature summary for 3.1.11.3 extracted from

  • Kerr, C.; Sadowski, P.D.
    Gene 6 exonuclease of bacteriophage T7. I. Purification and properties of the enzyme (1972), J. Biol. Chem., 247, 305-310.
    View publication on PubMed
Search for more literature for 3.1.11.3

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol required Escherichia coli
dithiothreitol required Escherichia phage T7
KCl stimulates at 20 mM Escherichia coli
KCl stimulates at 20 mM Escherichia phage T7

General Stability

General Stability Organism
stability enhanced by dithiothreitol Escherichia coli
stability enhanced by dithiothreitol Escherichia phage T7

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Escherichia coli
Mg2+ required Escherichia phage T7
Mg2+ optimal concentration 5 mM Escherichia coli
Mg2+ optimal concentration 5 mM Escherichia phage T7
Mn2+ divalent cation: Mn2+ or Mg2+ required Escherichia coli
Mn2+ divalent cation: Mn2+ or Mg2+ required Escherichia phage T7
Mn2+ optimal concentration 1 mM Escherichia coli
Mn2+ optimal concentration 1 mM Escherichia phage T7

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
double-stranded DNA + H2O Escherichia coli
-
 single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
 ?
double-stranded DNA + H2O Escherichia phage T7
-
 single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 Escherichia coli infected with phage lambda only
-
Escherichia phage T7
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Escherichia coli
partial Escherichia phage T7

Storage Stability

Storage Stability Organism
-20°C, 40% glycerol, 1mg/ml bovine serum albumin, 6 months, 0% loss of activity Escherichia coli
-20°C, 40% glycerol, 1mg/ml bovine serum albumin, 6 months, 0% loss of activity Escherichia phage T7

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
double-stranded DNA + H2O
-
 Escherichia coli single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
 ?
double-stranded DNA + H2O
-
 Escherichia phage T7 single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
 ?
double-stranded DNA + H2O degradation also at single-stranded breaks within DNA-duplex Escherichia coli single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
 ?
double-stranded DNA + H2O degradation also at single-stranded breaks within DNA-duplex Escherichia phage T7 single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
 ?
double-stranded DNA + H2O degrades double-stranded native DNA Escherichia coli single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
 ?
double-stranded DNA + H2O degrades double-stranded native DNA Escherichia phage T7 single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
 ?