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Literature summary for 3.1.11.3 extracted from

  • Little, J.W.
    Lambda exonuclease (1981), Gene Amplif. Anal., 2, 135-145.
    View publication on PubMed
Search for more literature for 3.1.11.3

Application

Application Comment Organism
analysis use as reagent restriction mapping and substrate preparation Escherichia coli
analysis use as reagent restriction mapping and substrate preparation Lambdavirus lambda
analysis for studies of genetic recombination Escherichia coli
analysis for studies of genetic recombination Lambdavirus lambda
analysis use as reagent for determination of secondary structures at DNA termini Escherichia coli
analysis use as reagent for determination of secondary structures at DNA termini Lambdavirus lambda

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
double-stranded DNA + H2O Escherichia coli
-
 single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
 ?
double-stranded DNA + H2O Lambdavirus lambda
-
 single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 Escherichia coli infected with phage lambda only
-
Lambdavirus lambda
-
 multiple mutant phage strain SG5519
-
Lambdavirus lambda
-
 redA and redB mutants
-
Lambdavirus lambda
-
 defective lambda mutant T11
-

Purification (Commentary)

Purification (Comment) Organism
lambda mutant T11 overexpress the enzyme Escherichia coli
lambda mutant T11 overexpress the enzyme Lambdavirus lambda
multiple mutant phage strain SG5519 as source of the enzyme Escherichia coli
multiple mutant phage strain SG5519 as source of the enzyme Lambdavirus lambda

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
double-stranded DNA + H2O
-
 Escherichia coli single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
 ?
double-stranded DNA + H2O
-
 Lambdavirus lambda single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
 ?
double-stranded DNA + H2O exonucleolytic cleavage in the 5'- to 3'-direction to yield 5'-phosphomononucleotides Escherichia coli single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
 ?
double-stranded DNA + H2O exonucleolytic cleavage in the 5'- to 3'-direction to yield 5'-phosphomononucleotides Lambdavirus lambda single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
 ?
double-stranded DNA + H2O possess "red" recombination system Escherichia coli single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
 ?
double-stranded DNA + H2O possess "red" recombination system Lambdavirus lambda single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
 ?
double-stranded DNA + H2O high processivity Escherichia coli single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
 ?
double-stranded DNA + H2O high processivity Lambdavirus lambda single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
 ?
double-stranded DNA + H2O degrades native DNA 100 times faster than long single-stranded DNA or oligonucleotides Escherichia coli single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
 ?
double-stranded DNA + H2O degrades native DNA 100 times faster than long single-stranded DNA or oligonucleotides Lambdavirus lambda single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
 ?
double-stranded DNA + H2O shows strong preference for termini bearing 5'-phosphoryl groups Escherichia coli single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
 ?
double-stranded DNA + H2O shows strong preference for termini bearing 5'-phosphoryl groups Lambdavirus lambda single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
 ?
double-stranded DNA + H2O no attack at single-stranded breaks within DNA-duplex Escherichia coli single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
 ?
double-stranded DNA + H2O no attack at single-stranded breaks within DNA-duplex Lambdavirus lambda single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
 ?
double-stranded DNA + H2O degrades double-stranded native DNA Escherichia coli single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
 ?
double-stranded DNA + H2O degrades double-stranded native DNA Lambdavirus lambda single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
 ?
double-stranded DNA + H2O preference for a 5'-phosphoryl group over a 5'-hydroxyl group Escherichia coli single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
 ?
double-stranded DNA + H2O preference for a 5'-phosphoryl group over a 5'-hydroxyl group Lambdavirus lambda single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
 ?
oligonucleotides + H2O strong preference for relatively short chain oligonucleotides Escherichia coli 5'-phosphomononucleotides
-
 ?
oligonucleotides + H2O strong preference for relatively short chain oligonucleotides Lambdavirus lambda 5'-phosphomononucleotides
-
 ?
oligonucleotides + H2O low rate Escherichia coli 5'-phosphomononucleotides
-
 ?
oligonucleotides + H2O low rate Lambdavirus lambda 5'-phosphomononucleotides
-
 ?