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Information on EC 2.1.1.60 - calmodulin-lysine N-methyltransferase
for references in articles please use BRENDA:EC2.1.1.60
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EC Tree 2 Transferases
2.1 Transferring one-carbon groups
2.1.1 Methyltransferases
2.1.1.60 calmodulin-lysine N-methyltransferase
IUBMB Comments
One of a group of enzymes methylating proteins; see also the family of histone-lysine N-methyltransferases (previously EC 2.1.1.43) and EC 2.1.1.59 [cytochrome c]-lysine N-methyltransferase.
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
- 2.1.1.60
- calmodulins
-
trimethylated
-
unmethylated
-
calmodulin-dependent
-
facial
- calmidazolium
-
panic
-
phobias
-
hypotonia
-
dysmorphic
- trimethyllysine
- paramecium
-
cystinuria
-
calcium-depleted
-
calmodulin-binding
-
dsm-based
-
calmodulin-sepharose
Reaction Schemes
showSynonyms
cam kmt, calmodulin n-methyltransferase, calmodulin methyltransferase, camkmt, calmodulin-lysine n-methyltransferase, s-adenosyl-l-methionine:calmodulin-l-lysine n6-methyltransferase, calmodulin lysine methyltransferase, calmodulin lysine methyl transferase, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
calmodulin lysine methyltransferase
calmodulin lysine N-methyltransferase
-
-
-
-
calmodulin methyltransferase
calmodulin N-methyltransferase
calmodulin-lysine N-methyltransferase
CaM KMT
CaMKMT
methyltransferase, calmodulin (lysine)
-
-
-
-
S-adenosylmethionine:calmodulin N-methyltransferase (lysine)
-
-
-
-
calmodulin N-methyltransferase
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + calmodulin L-lysine = S-adenosyl-L-homocysteine + calmodulin N6-methyl-L-lysine
S-adenosyl-L-methionine + calmodulin L-lysine = S-adenosyl-L-homocysteine + calmodulin N6-methyl-L-lysine
Bi Bi sequential mechanism
-
S-adenosyl-L-methionine + calmodulin L-lysine = S-adenosyl-L-homocysteine + calmodulin N6-methyl-L-lysine
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
methyl group transfer
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
KEGG
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:calmodulin-L-lysine N6-methyltransferase
One of a group of enzymes methylating proteins; see also the family of histone-lysine N-methyltransferases (previously EC 2.1.1.43) and EC 2.1.1.59 [cytochrome c]-lysine N-methyltransferase.
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CAS REGISTRY NUMBER
COMMENTARY
75603-20-2
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + calmodulin L-lysine
S-adenosyl-L-homocysteine + calmodulin N6-methyl-L-lysine
S-adenosyl-L-methionine + calmodulin L-lysine-115
S-adenosyl-L-homocysteine + calmodulin N6-methyl-L-lysine-115
S-adenosyl-L-methionine + mushroom demethylcalmodulin
?
-
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + Octopus calmodulin L-lysine
S-adenosyl-L-homocysteine + Octopus calmodulin N6-methyl-L-lysine
-
Substrates: -
Products: -
Products: -
r
S-adenosyl-L-methionine + VU-1 calmodulin L-lysine
S-adenosyl-L-homocysteine + VU-1 calmodulin N6-methyl-L-lysine
-
Substrates: -
Products: -
Products: -
r
S-adenosyl-L-methionine + calmodulin L-lysine
S-adenosyl-L-homocysteine + calmodulin N6-methyl-L-lysine
-
Substrates: the enzyme transfers three methyl groups to a highly conserved lysyl residue at position 115 in calmodulin
Products: -
Products: -
?
S-adenosyl-L-methionine + calmodulin L-lysine
S-adenosyl-L-homocysteine + calmodulin N6-methyl-L-lysine
-
Substrates: calmodulin is methylated at Lys315
Products: -
Products: -
?
S-adenosyl-L-methionine + calmodulin L-lysine
S-adenosyl-L-homocysteine + calmodulin N6-methyl-L-lysine
-
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + calmodulin L-lysine
S-adenosyl-L-homocysteine + calmodulin N6-methyl-L-lysine
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + calmodulin L-lysine
S-adenosyl-L-homocysteine + calmodulin N6-methyl-L-lysine
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + calmodulin L-lysine
S-adenosyl-L-homocysteine + calmodulin N6-methyl-L-lysine
-
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + calmodulin L-lysine
S-adenosyl-L-homocysteine + calmodulin N6-methyl-L-lysine
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + calmodulin L-lysine
S-adenosyl-L-homocysteine + calmodulin N6-methyl-L-lysine
-
Substrates: Pisum sativum calmodulin
Products: -
Products: -
?
S-adenosyl-L-methionine + calmodulin L-lysine
S-adenosyl-L-homocysteine + calmodulin N6-methyl-L-lysine
-
Substrates: posttranslational modification of calmodulin
Products: -
Products: -
?
S-adenosyl-L-methionine + calmodulin L-lysine
S-adenosyl-L-homocysteine + calmodulin N6-methyl-L-lysine
-
Substrates: -
Products: trimethyllysine calmodulin, major product, + dimethyllysine + monomethyllysine
Products: trimethyllysine calmodulin, major product, + dimethyllysine + monomethyllysine
?
S-adenosyl-L-methionine + calmodulin L-lysine
S-adenosyl-L-homocysteine + calmodulin N6-methyl-L-lysine
-
Substrates: highly specific
Products: -
Products: -
?
S-adenosyl-L-methionine + calmodulin L-lysine
S-adenosyl-L-homocysteine + calmodulin N6-methyl-L-lysine
-
Substrates: highly specific
Products: 3 mol of methyl per mol of calmodulin are incorporated into lysine 115 of des(methyl) calmodulin from Dictyostelium discoideum resulting in formation of 1 mol of trimethyllysine at the site normally methylated
Products: 3 mol of methyl per mol of calmodulin are incorporated into lysine 115 of des(methyl) calmodulin from Dictyostelium discoideum resulting in formation of 1 mol of trimethyllysine at the site normally methylated
?
S-adenosyl-L-methionine + calmodulin L-lysine
S-adenosyl-L-homocysteine + calmodulin N6-methyl-L-lysine
-
Substrates: highly specific
Products: trimethyllysine calmodulin, major product, + dimethyllysine + monomethyllysine
Products: trimethyllysine calmodulin, major product, + dimethyllysine + monomethyllysine
?
S-adenosyl-L-methionine + calmodulin L-lysine
S-adenosyl-L-homocysteine + calmodulin N6-methyl-L-lysine
-
Substrates: Dictyostelium discoideum calmodulin
Products: 3 mol of methyl per mol of calmodulin are incorporated into lysine 115 of des(methyl) calmodulin from Dictyostelium discoideum resulting in formation of 1 mol of trimethyllysine at the site normally methylated
Products: 3 mol of methyl per mol of calmodulin are incorporated into lysine 115 of des(methyl) calmodulin from Dictyostelium discoideum resulting in formation of 1 mol of trimethyllysine at the site normally methylated
?
S-adenosyl-L-methionine + calmodulin L-lysine
S-adenosyl-L-homocysteine + calmodulin N6-methyl-L-lysine
-
Substrates: octopus calmodulin
Products: enzyme methylates a specific lysine residue of octopus calmodulin
Products: enzyme methylates a specific lysine residue of octopus calmodulin
?
S-adenosyl-L-methionine + calmodulin L-lysine
S-adenosyl-L-homocysteine + calmodulin N6-methyl-L-lysine
-
Substrates: octopus calmodulin
Products: formation of epsilon-N-mono-, epsilon-N-di- and epsilon-N-trimethyllysine, the labelled N-methyllysine lies in the 107-126 peptide
Products: formation of epsilon-N-mono-, epsilon-N-di- and epsilon-N-trimethyllysine, the labelled N-methyllysine lies in the 107-126 peptide
?
S-adenosyl-L-methionine + calmodulin L-lysine
S-adenosyl-L-homocysteine + calmodulin N6-methyl-L-lysine
-
Substrates: mushroom demthylcalmodulin
Products: trimethyllysine calmodulin, major product, + dimethyllysine + monomethyllysine
Products: trimethyllysine calmodulin, major product, + dimethyllysine + monomethyllysine
?
S-adenosyl-L-methionine + calmodulin L-lysine
S-adenosyl-L-homocysteine + calmodulin N6-methyl-L-lysine
-
Substrates: posttranslational modification of calmodulin
Products: -
Products: -
?
S-adenosyl-L-methionine + calmodulin L-lysine-115
S-adenosyl-L-homocysteine + calmodulin N6-methyl-L-lysine-115
-
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + calmodulin L-lysine-115
S-adenosyl-L-homocysteine + calmodulin N6-methyl-L-lysine-115
-
Substrates: -
Products: -
Products: -
?
additional information
?
-
-
Substrates: structural requirements of calmodulin
Products: -
Products: -
?
additional information
?
-
-
Substrates: troponin C has low activity at very high troponin concentration
Products: -
Products: -
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + calmodulin L-lysine
S-adenosyl-L-homocysteine + calmodulin N6-methyl-L-lysine
S-adenosyl-L-methionine + calmodulin L-lysine
S-adenosyl-L-homocysteine + calmodulin N6-methyl-L-lysine
-
Substrates: the enzyme transfers three methyl groups to a highly conserved lysyl residue at position 115 in calmodulin
Products: -
Products: -
?
S-adenosyl-L-methionine + calmodulin L-lysine
S-adenosyl-L-homocysteine + calmodulin N6-methyl-L-lysine
-
Substrates: calmodulin is methylated at Lys315
Products: -
Products: -
?
S-adenosyl-L-methionine + calmodulin L-lysine
S-adenosyl-L-homocysteine + calmodulin N6-methyl-L-lysine
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + calmodulin L-lysine
S-adenosyl-L-homocysteine + calmodulin N6-methyl-L-lysine
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + calmodulin L-lysine
S-adenosyl-L-homocysteine + calmodulin N6-methyl-L-lysine
-
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + calmodulin L-lysine
S-adenosyl-L-homocysteine + calmodulin N6-methyl-L-lysine
-
Substrates: posttranslational modification of calmodulin
Products: -
Products: -
?
S-adenosyl-L-methionine + calmodulin L-lysine
S-adenosyl-L-homocysteine + calmodulin N6-methyl-L-lysine
-
Substrates: posttranslational modification of calmodulin
Products: -
Products: -
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ca2+
Mg2+
Mn2+
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
-
not inhibitory: sinefungin, tubercidin, calmodulin antagonist W7
-
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
[Thr136]calmodulin
-
defective in Ca-binding, cannot be methylated by the enzyme
-
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DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Adenocarcinoma, Mucinous
A Novel CAMKMT Exon3-ALK Exon20 Fusion Variant was Identified in a Primary Pulmonary Mucinous Adenocarcinoma.
Intellectual Disability
Calmodulin Methyltransferase Is Required for Growth, Muscle Strength, Somatosensory Development and Brain Function.
Muscle Weakness
Calmodulin Methyltransferase Is Required for Growth, Muscle Strength, Somatosensory Development and Brain Function.
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
highest transcript expression is revealed in the root tip followed by flower and new leaf, while, the least expression is found in the old leaf
brenda
highest transcript expression is revealed in the root tip followed by flower and new leaf, while, the least expression is found in the old leaf. Strong OsCaM KMT promoter expression in midribs, stomata, and tip of leaf tissues
brenda
highest transcript expression is revealed in the root tip followed by flower and new leaf, while, the least expression is found in the old leaf. Strong OsCaM KMT promoter expression in central cylinder as well as tip parts of the primary root and lateral roots
brenda
additional information
-
found at high levels in tissues with high levels of calmodulin, at low levels in tissues with low levels of calmodulin like liver, heart, skeletal muscle
brenda
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Highest Expressing Human Cell Lines
Cell Line LinksPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
metabolism
the enzyme is the major methyltransferase of calmodulin in human cells and a Hsp90 client protein
physiological function
malfunction
enzyme-deficient mice have deficits in motor learning, complex coordination and learning of aversive stimuli
malfunction
the absence of enzyme causes accumulation of hypomethylated calmodulin in 2p21 deletion syndrome patients
physiological function
the enzyme is essential for normal body growth and somatosensory development, as well as for the proper functioning of the adult mouse brain
physiological function
the enzyme plays an anti-inflammatory role through restraining caspase-11 non-canonical inflammasome in macrophages
Show AA Sequence and Transmembrane Helices (1959 entries)
Please use the AA Sequence and Transmembrane Helices Search for a specific query.
Please use the AA Sequence and Transmembrane Helices Search for a specific query.
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PDB
SCOP
CATH
UNIPROT
ORGANISM
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
36000
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Arabidopsis thaliana OX-1 and OX-7 strains in root tips, floral buds, stamens, apical meristems, and germinating seeds
-
expressed in Escherichia coli
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
highest transcript expression is revealed in the root tip followed by flower and new leaf, while, the least expression is found in the old leaf
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Sitaramayya, A.; Wright, L.S.; Siegel, F.L.
Enzymatic methylation of calmodulin in rat brain cytosol
J. Biol. Chem.
255
8894-8900
1980
Rattus norvegicus
brenda
Murtaugh, T.J.; Rowe, P.M.; Vincent, P.L.; Wright, L.S.; Siegel, F.L.
Posttranslational modification of calmodulin
Methods Enzymol.
102
158-170
1983
Rattus norvegicus
brenda
Rowe, P.M.; Wright, L.S.; Siegel, F.L.
Calmodulin N-methyltransferase. Partial purification and characterization
J. Biol. Chem.
261
7060-7069
1986
Rattus norvegicus
brenda
Oh, S.H.; Roberts, D.M.
Analysis of the state of posttranslational calmodulin methylation in developing pea plants
Plant Physiol.
93
880-887
1990
Ovis aries
brenda
Morino, H.; Kawamoto, T.; Miyake, M.; Kakimoto, Y.
Purification and properties of calmodulin-lysine N-methyltransferase from rat brain cytosol
J. Neurochem.
48
1201-1208
1987
Rattus norvegicus
brenda
Han, C.H.; Roberts, D.M.
Altered methylation substrate kinetics and calcium binding of a calmodulin with a Val136->Thr substitution
Eur. J. Biochem.
244
904-912
1997
Escherichia coli
brenda
Pech, L.L.; Nelson, D.L.
Purification and characterization of calmodulin (lysine 115) N-methyltransferase from Paramecium tetraurelia
Biochim. Biophys. Acta
1199
183-194
1994
Paramecium tetraurelia
brenda
Wright, L.S.; Bertics, P.J.; Siegel, F.L.
Calmodulin N-methyltransferase. Kinetics, mechanism, and inhibitors
J. Biol. Chem.
271
12737-12743
1996
Escherichia coli, Rattus norvegicus
brenda
Cobb, J.A.; Roberts, D.M.
Structural requirements for N-trimethylation of lysine 115 of calmodulin
J. Biol. Chem.
275
18969-18975
2000
Escherichia coli
brenda
Magnani, R.; Dirk, L.M.; Trievel, R.C.; Houtz, R.L.
Calmodulin methyltransferase is an evolutionarily conserved enzyme that trimethylates Lys-115 in calmodulin
Nat. Commun.
1
43
2010
Homo sapiens, Rattus norvegicus
brenda
Banerjee, J.; Magnani, R.; Nair, M.; Dirk, L.M.; DeBolt, S.; Maiti, I.B.; Houtz, R.L.
Calmodulin-mediated signal transduction pathways in Arabidopsis are fine-tuned by methylation
Plant Cell
25
4493-4511
2013
Arabidopsis thaliana
brenda
Haziza, S.; Magnani, R.; Lan, D.; Keinan, O.; Saada, A.; Hershkovitz, E.; Yanay, N.; Cohen, Y.; Nevo, Y.; Houtz, R.L.; Sheffield, V.C.; Golan, H.; Parvari, R.
Calmodulin methyltransferase is required for growth, muscle strength, somatosensory development and brain function
PLoS Genet.
11
e1005388
2015
Mus musculus (Q3U2J5), Mus musculus
brenda
Magen, S.; Magnani, R.; Haziza, S.; Hershkovitz, E.; Houtz, R.; Cambi, F.; Parvari, R.
Human calmodulin methyltransferase: expression, activity on calmodulin, and Hsp90 dependence
PLoS ONE
7
e52425
2012
Homo sapiens (Q7Z624), Homo sapiens, Mus musculus (Q3U2J5), Mus musculus
brenda
Magnani, R.; Chaffin, B.; Dick, E.; Bricken, M.L.; Houtz, R.L.; Bradley, L.H.
Utilization of a calmodulin lysine methyltransferase co-expression system for the generation of a combinatorial library of post-translationally modified proteins
Protein Expr. Purif.
86
83-88
2012
Homo sapiens (Q7Z624)
brenda
Serre, N.B.C.; Sarthou, M.; Gigarel, O.; Figuet, S.; Corso, M.; Choulet, J.; Rofidal, V.; Alban, C.; Santoni, V.; Bourguignon, J.; Verbruggen, N.; Ravanel, S.
Protein lysine methylation contributes to modulating the response of sensitive and tolerant Arabidopsis species to cadmium stress
Plant Cell Environ.
43
760-774
2020
Arabidopsis thaliana
brenda
Cho, H.J.; Lee, D.J.; Yi, Y.S.
Anti-inflammatory activity of calmodulin-lysine N-methyltransferase through suppressing the caspase-11 non-canonical inflammasome
Immunobiology
228
152758
2023
Mus musculus (Q3U2J5)
brenda
Nandi, D.; Gain, H.; Paul, S.; Mitra, A.; Banerjee, J.
Bioinformatics analyses of rice calmodulin-lysine N-methyltransferases gene (OsCaM KMT) and characterization of its promoter from Oryza sativa L. sub sp. indica through transgenic approach
South Afr. J. Bot.
150
873-885
2022
Oryza sativa Japonica Group (Q10P78)
-
brenda
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